TY  - CONF
AU  - Nedović, Viktor
AU  - Obradović, B
AU  - Poncelet, Denis
AU  - Goosen, MFA
AU  - Leskošek-Čukalović, Ida
AU  - Bugarski, Branko
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/506
AB  - This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.
PB  - Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig
C3  - Practical Aspects of Encapsulation Technologies
T1  - Cell immobilisation by electrostatic droplet generation
EP  - 17
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_506
ER  - 

@conference{
author = "Nedović, Viktor and Obradović, B and Poncelet, Denis and Goosen, MFA and Leskošek-Čukalović, Ida and Bugarski, Branko",
year = "2002",
abstract = "This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.",
publisher = "Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig",
journal = "Practical Aspects of Encapsulation Technologies",
title = "Cell immobilisation by electrostatic droplet generation",
pages = "17-11",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_506"
}

Nedović, V., Obradović, B., Poncelet, D., Goosen, M., Leskošek-Čukalović, I.,& Bugarski, B.. (2002). Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies
Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig., 11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506

Nedović V, Obradović B, Poncelet D, Goosen M, Leskošek-Čukalović I, Bugarski B. Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies. 2002;:11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506 .

Nedović, Viktor, Obradović, B, Poncelet, Denis, Goosen, MFA, Leskošek-Čukalović, Ida, Bugarski, Branko, "Cell immobilisation by electrostatic droplet generation" in Practical Aspects of Encapsulation Technologies (2002):11-17,
https://hdl.handle.net/21.15107/rcub_agrospace_506 .

TY  - JOUR
AU  - Pjanović, Rada
AU  - Goosen, MFA
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2000
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/234
AB  - The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.
PB  - Edizioni Minerva Medica, Turin
T2  - Minerva Biotecnologica
T1  - Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory
EP  - 248
IS  - 4
SP  - 241
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_234
ER  - 

@article{
author = "Pjanović, Rada and Goosen, MFA and Nedović, Viktor and Bugarski, Branko",
year = "2000",
abstract = "The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.",
publisher = "Edizioni Minerva Medica, Turin",
journal = "Minerva Biotecnologica",
title = "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory",
pages = "248-241",
number = "4",
volume = "12",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_234"
}

Pjanović, R., Goosen, M., Nedović, V.,& Bugarski, B.. (2000). Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica
Edizioni Minerva Medica, Turin., 12(4), 241-248.
https://hdl.handle.net/21.15107/rcub_agrospace_234

Pjanović R, Goosen M, Nedović V, Bugarski B. Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica. 2000;12(4):241-248.
https://hdl.handle.net/21.15107/rcub_agrospace_234 .

Pjanović, Rada, Goosen, MFA, Nedović, Viktor, Bugarski, Branko, "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory" in Minerva Biotecnologica, 12, no. 4 (2000):241-248,
https://hdl.handle.net/21.15107/rcub_agrospace_234 .