TY  - JOUR
AU  - Pillai Balamurugan, Joshini
AU  - Supramani, Sugenendran
AU  - Usuldin, Siti Rokhiyah Ahmad
AU  - Ilham, Zul
AU  - Klaus, Anita
AU  - Kusaira Khairul Ikram, Nur
AU  - Ahmad, Rahayu
AU  - Wan-Mohtarach, Wan Abd Al Qadr Imad
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5943
AB  - A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (Knuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 °C, 25 °C and 30 °C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20°C–30 °C), agitation rate (100–200 rpm), glucose concentration (10 g/L–50 g/L) and initial pH (4–6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 °C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm−1, 1623 cm−1, 1073 cm−1, 890 cm−1) and NMR (δ 4.30, 4.80, 5.20 ppm) as endo-β-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-β-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.
PB  - Elsevier Ltd
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation
SP  - 102166
VL  - 37
DO  - 10.1016/j.bcab.2021.102166
ER  - 

@article{
author = "Pillai Balamurugan, Joshini and Supramani, Sugenendran and Usuldin, Siti Rokhiyah Ahmad and Ilham, Zul and Klaus, Anita and Kusaira Khairul Ikram, Nur and Ahmad, Rahayu and Wan-Mohtarach, Wan Abd Al Qadr Imad",
year = "2021",
abstract = "A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (Knuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 °C, 25 °C and 30 °C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20°C–30 °C), agitation rate (100–200 rpm), glucose concentration (10 g/L–50 g/L) and initial pH (4–6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 °C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm−1, 1623 cm−1, 1073 cm−1, 890 cm−1) and NMR (δ 4.30, 4.80, 5.20 ppm) as endo-β-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-β-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.",
publisher = "Elsevier Ltd",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation",
pages = "102166",
volume = "37",
doi = "10.1016/j.bcab.2021.102166"
}

Pillai Balamurugan, J., Supramani, S., Usuldin, S. R. A., Ilham, Z., Klaus, A., Kusaira Khairul Ikram, N., Ahmad, R.,& Wan-Mohtarach, W. A. A. Q. I.. (2021). Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology
Elsevier Ltd., 37, 102166.
https://doi.org/10.1016/j.bcab.2021.102166

Pillai Balamurugan J, Supramani S, Usuldin SRA, Ilham Z, Klaus A, Kusaira Khairul Ikram N, Ahmad R, Wan-Mohtarach WAAQI. Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2021;37:102166.
doi:10.1016/j.bcab.2021.102166 .

Pillai Balamurugan, Joshini, Supramani, Sugenendran, Usuldin, Siti Rokhiyah Ahmad, Ilham, Zul, Klaus, Anita, Kusaira Khairul Ikram, Nur, Ahmad, Rahayu, Wan-Mohtarach, Wan Abd Al Qadr Imad, "Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 37 (2021):102166,
https://doi.org/10.1016/j.bcab.2021.102166 . .

TY  - JOUR
AU  - Supramani, Sugenendran
AU  - Ahmad, Rahayu
AU  - Ilham, Zul
AU  - Annuar, Mohamad Suffian Mohamad
AU  - Klaus, Anita
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5018
AB  - Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.
PB  - Amer Inst Mathematical Sciences-Aims, Springfield
T2  - Aims Microbiology
T1  - Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology
EP  - 38
IS  - 1
SP  - 19
VL  - 5
DO  - 10.3934/microbiol.2019.1.19
ER  - 

@article{
author = "Supramani, Sugenendran and Ahmad, Rahayu and Ilham, Zul and Annuar, Mohamad Suffian Mohamad and Klaus, Anita and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.",
publisher = "Amer Inst Mathematical Sciences-Aims, Springfield",
journal = "Aims Microbiology",
title = "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology",
pages = "38-19",
number = "1",
volume = "5",
doi = "10.3934/microbiol.2019.1.19"
}

Supramani, S., Ahmad, R., Ilham, Z., Annuar, M. S. M., Klaus, A.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology
Amer Inst Mathematical Sciences-Aims, Springfield., 5(1), 19-38.
https://doi.org/10.3934/microbiol.2019.1.19

Supramani S, Ahmad R, Ilham Z, Annuar MSM, Klaus A, Wan-Mohtar WAAQI. Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology. 2019;5(1):19-38.
doi:10.3934/microbiol.2019.1.19 .

Supramani, Sugenendran, Ahmad, Rahayu, Ilham, Zul, Annuar, Mohamad Suffian Mohamad, Klaus, Anita, Wan-Mohtar, Wan Abd Al Qadr Imad, "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology" in Aims Microbiology, 5, no. 1 (2019):19-38,
https://doi.org/10.3934/microbiol.2019.1.19 . .

TY  - JOUR
AU  - Supramani, Sugenendran
AU  - Jailani, Nurulhuda
AU  - Ramarao, Kivaandra
AU  - Zain, Nurul Amalina Mohd
AU  - Klaus, Anita
AU  - Ahmad, Rahayu
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4959
AB  - The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 degrees C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day(-1)) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 mu m  lt  d  lt  40.67 mu m) were associated with high DCW while small dispersed pellets (diameter: 11.67 mu m  lt  d  lt  14.67 mu m) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 mu m  lt  d  lt  7.67 mu m) to RBF4 (16.67 mu m  lt  d  lt  18.33 mu m). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day(-1) to 0.108 g/L day(-1). In an FTIR analysis, crude EPS of EGP showed the presence of beta-glucan characteristics at 1075 cm(-1) and 891 cm(-1) wavelengths, similar with European G. lucidum BCCM 31549. In addition, H-1 NMR showed similar beta-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production.
PB  - Elsevier, Amsterdam
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production
VL  - 19
DO  - 10.1016/j.bcab.2019.101118
ER  - 

@article{
author = "Supramani, Sugenendran and Jailani, Nurulhuda and Ramarao, Kivaandra and Zain, Nurul Amalina Mohd and Klaus, Anita and Ahmad, Rahayu and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 degrees C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day(-1)) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 mu m  lt  d  lt  40.67 mu m) were associated with high DCW while small dispersed pellets (diameter: 11.67 mu m  lt  d  lt  14.67 mu m) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 mu m  lt  d  lt  7.67 mu m) to RBF4 (16.67 mu m  lt  d  lt  18.33 mu m). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day(-1) to 0.108 g/L day(-1). In an FTIR analysis, crude EPS of EGP showed the presence of beta-glucan characteristics at 1075 cm(-1) and 891 cm(-1) wavelengths, similar with European G. lucidum BCCM 31549. In addition, H-1 NMR showed similar beta-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production.",
publisher = "Elsevier, Amsterdam",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production",
volume = "19",
doi = "10.1016/j.bcab.2019.101118"
}

Supramani, S., Jailani, N., Ramarao, K., Zain, N. A. M., Klaus, A., Ahmad, R.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production. in Biocatalysis and Agricultural Biotechnology
Elsevier, Amsterdam., 19.
https://doi.org/10.1016/j.bcab.2019.101118

Supramani S, Jailani N, Ramarao K, Zain NAM, Klaus A, Ahmad R, Wan-Mohtar WAAQI. Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production. in Biocatalysis and Agricultural Biotechnology. 2019;19.
doi:10.1016/j.bcab.2019.101118 .

Supramani, Sugenendran, Jailani, Nurulhuda, Ramarao, Kivaandra, Zain, Nurul Amalina Mohd, Klaus, Anita, Ahmad, Rahayu, Wan-Mohtar, Wan Abd Al Qadr Imad, "Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production" in Biocatalysis and Agricultural Biotechnology, 19 (2019),
https://doi.org/10.1016/j.bcab.2019.101118 . .