TY  - JOUR
AU  - Pillai Balamurugan, Joshini
AU  - Supramani, Sugenendran
AU  - Usuldin, Siti Rokhiyah Ahmad
AU  - Ilham, Zul
AU  - Klaus, Anita
AU  - Kusaira Khairul Ikram, Nur
AU  - Ahmad, Rahayu
AU  - Wan-Mohtarach, Wan Abd Al Qadr Imad
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5943
AB  - A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (Knuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 °C, 25 °C and 30 °C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20°C–30 °C), agitation rate (100–200 rpm), glucose concentration (10 g/L–50 g/L) and initial pH (4–6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 °C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm−1, 1623 cm−1, 1073 cm−1, 890 cm−1) and NMR (δ 4.30, 4.80, 5.20 ppm) as endo-β-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-β-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.
PB  - Elsevier Ltd
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation
SP  - 102166
VL  - 37
DO  - 10.1016/j.bcab.2021.102166
ER  - 

@article{
author = "Pillai Balamurugan, Joshini and Supramani, Sugenendran and Usuldin, Siti Rokhiyah Ahmad and Ilham, Zul and Klaus, Anita and Kusaira Khairul Ikram, Nur and Ahmad, Rahayu and Wan-Mohtarach, Wan Abd Al Qadr Imad",
year = "2021",
abstract = "A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (Knuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 °C, 25 °C and 30 °C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20°C–30 °C), agitation rate (100–200 rpm), glucose concentration (10 g/L–50 g/L) and initial pH (4–6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 °C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm−1, 1623 cm−1, 1073 cm−1, 890 cm−1) and NMR (δ 4.30, 4.80, 5.20 ppm) as endo-β-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-β-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.",
publisher = "Elsevier Ltd",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation",
pages = "102166",
volume = "37",
doi = "10.1016/j.bcab.2021.102166"
}

Pillai Balamurugan, J., Supramani, S., Usuldin, S. R. A., Ilham, Z., Klaus, A., Kusaira Khairul Ikram, N., Ahmad, R.,& Wan-Mohtarach, W. A. A. Q. I.. (2021). Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology
Elsevier Ltd., 37, 102166.
https://doi.org/10.1016/j.bcab.2021.102166

Pillai Balamurugan J, Supramani S, Usuldin SRA, Ilham Z, Klaus A, Kusaira Khairul Ikram N, Ahmad R, Wan-Mohtarach WAAQI. Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2021;37:102166.
doi:10.1016/j.bcab.2021.102166 .

Pillai Balamurugan, Joshini, Supramani, Sugenendran, Usuldin, Siti Rokhiyah Ahmad, Ilham, Zul, Klaus, Anita, Kusaira Khairul Ikram, Nur, Ahmad, Rahayu, Wan-Mohtarach, Wan Abd Al Qadr Imad, "Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 37 (2021):102166,
https://doi.org/10.1016/j.bcab.2021.102166 . .

TY  - JOUR
AU  - Hassan, Nurul Amirah
AU  - Supramani, Sugenendran
AU  - Sohedein, Mohamad Nor Azzimi
AU  - Usuldin, Siti Rokhiyah Ahmad
AU  - Klaus, Anita
AU  - Ilham, Zul
AU  - Chen, Wei-Hsin
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5007
AB  - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
PB  - Elsevier, Amsterdam
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation
VL  - 21
DO  - 10.1016/j.bcab.2019.101305
ER  - 

@article{
author = "Hassan, Nurul Amirah and Supramani, Sugenendran and Sohedein, Mohamad Nor Azzimi and Usuldin, Siti Rokhiyah Ahmad and Klaus, Anita and Ilham, Zul and Chen, Wei-Hsin and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.",
publisher = "Elsevier, Amsterdam",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation",
volume = "21",
doi = "10.1016/j.bcab.2019.101305"
}

Hassan, N. A., Supramani, S., Sohedein, M. N. A., Usuldin, S. R. A., Klaus, A., Ilham, Z., Chen, W.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology
Elsevier, Amsterdam., 21.
https://doi.org/10.1016/j.bcab.2019.101305

Hassan NA, Supramani S, Sohedein MNA, Usuldin SRA, Klaus A, Ilham Z, Chen W, Wan-Mohtar WAAQI. Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2019;21.
doi:10.1016/j.bcab.2019.101305 .

Hassan, Nurul Amirah, Supramani, Sugenendran, Sohedein, Mohamad Nor Azzimi, Usuldin, Siti Rokhiyah Ahmad, Klaus, Anita, Ilham, Zul, Chen, Wei-Hsin, Wan-Mohtar, Wan Abd Al Qadr Imad, "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 21 (2019),
https://doi.org/10.1016/j.bcab.2019.101305 . .