TY  - JOUR
AU  - Puvača, Nikola
AU  - Ljubojević Pelić, Dragana
AU  - Pelić, Miloš
AU  - Bursić, Vojislava
AU  - Tufarelli, Vincenzo
AU  - Piemontese, Luca
AU  - Vuković, Gorica
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6270
AB  - The aim of this research was to investigate the effects of biofilm on antibiotic resistance of the bacterial isolates present in fish meat and to assess the risk of antibiotic residues for public health. Common carp, silver carp and grass carp fishes were purchased from retail stores for an in vitro biofilm investigation and a drug-resistant pattern determination. In all samples, up to 104 CFU/g of bacteria, such as Escherichia coli, Aeromonas hydrophila, Shewanella putrefaciens, Vibrio spp. and Staphylococcus spp., were observed. Isolates from the samples and their biofilms were subjected to an antibiogram assay using antibiotics such as amoxicillin, ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin, streptomycin, tetracycline and trimethoprim. Obtained results showed that some of the isolates were sensitive to antibiotics and some were resistant. Results of LC-MS/MS analysis showed that antibiotics residues were present in fish samples in the range between 4.9 and 199.4 µg/kg, with a total sum of 417.1 µg/kg. Estimated daily intake (EDI) was established to be 0.274 μg/kg of body weight/day for men and 0.332 μg/kg of body weight/day for women, with an acceptable daily intake (ADI) of 8.5 and 7.0 µg/kg of body weight/day for men and women, respectively. The results of the present study, therefore, highlight the safe consumption of fresh fish. © 2023 by the authors.
T2  - Antibiotics
T2  - Antibiotics
T1  - Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health
IS  - 1
VL  - 12
DO  - 10.3390/antibiotics12010143
ER  - 

@article{
author = "Puvača, Nikola and Ljubojević Pelić, Dragana and Pelić, Miloš and Bursić, Vojislava and Tufarelli, Vincenzo and Piemontese, Luca and Vuković, Gorica",
year = "2023",
abstract = "The aim of this research was to investigate the effects of biofilm on antibiotic resistance of the bacterial isolates present in fish meat and to assess the risk of antibiotic residues for public health. Common carp, silver carp and grass carp fishes were purchased from retail stores for an in vitro biofilm investigation and a drug-resistant pattern determination. In all samples, up to 104 CFU/g of bacteria, such as Escherichia coli, Aeromonas hydrophila, Shewanella putrefaciens, Vibrio spp. and Staphylococcus spp., were observed. Isolates from the samples and their biofilms were subjected to an antibiogram assay using antibiotics such as amoxicillin, ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin, streptomycin, tetracycline and trimethoprim. Obtained results showed that some of the isolates were sensitive to antibiotics and some were resistant. Results of LC-MS/MS analysis showed that antibiotics residues were present in fish samples in the range between 4.9 and 199.4 µg/kg, with a total sum of 417.1 µg/kg. Estimated daily intake (EDI) was established to be 0.274 μg/kg of body weight/day for men and 0.332 μg/kg of body weight/day for women, with an acceptable daily intake (ADI) of 8.5 and 7.0 µg/kg of body weight/day for men and women, respectively. The results of the present study, therefore, highlight the safe consumption of fresh fish. © 2023 by the authors.",
journal = "Antibiotics, Antibiotics",
title = "Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health",
number = "1",
volume = "12",
doi = "10.3390/antibiotics12010143"
}

Puvača, N., Ljubojević Pelić, D., Pelić, M., Bursić, V., Tufarelli, V., Piemontese, L.,& Vuković, G.. (2023). Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health. in Antibiotics, 12(1).
https://doi.org/10.3390/antibiotics12010143

Puvača N, Ljubojević Pelić D, Pelić M, Bursić V, Tufarelli V, Piemontese L, Vuković G. Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health. in Antibiotics. 2023;12(1).
doi:10.3390/antibiotics12010143 .

Puvača, Nikola, Ljubojević Pelić, Dragana, Pelić, Miloš, Bursić, Vojislava, Tufarelli, Vincenzo, Piemontese, Luca, Vuković, Gorica, "Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health" in Antibiotics, 12, no. 1 (2023),
https://doi.org/10.3390/antibiotics12010143 . .

TY  - JOUR
AU  - Roljević Nikolić, Svetlana
AU  - Dolijanović, Željko
AU  - Zecevic, Veselinka
AU  - Puvača, Nikola
AU  - Oljača, Snežana
AU  - Šeremešić, Srđan
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6090
AB  - The aim of the study was to examine the impact of microbiological and organic fertilizers on morphological and productive characteristics of triticale during a three-year period (2009/10–2011/12). A two-factorial field experiment was arranged using a randomized block design with four replications. The object of the study was the triticale winter cultivar Odisej, and the following treatments were applied: a control variant without fertilization, microbiological fertilizer “Slavol” (Agrounik Serbia) 5.0 l ha-1, organic fertilizer “Biohumus Royal offert” (Altamed RS) 3.0 t ha-1 + microbiological fertilizer “Slavol” (Agrounik Serbia) 5.0 l ha-1. The results showed that the expression of the characteristics was significantly affected by the environment. The lowest values were obtained in the first year when the most unfavourable meteorological conditions were observed. The application of microbiological fertilizer had no impact on the stem length and grain weight per spike, but it significantly increased the number of fertile spikelets (3.7%), spike length (7.7%) and grain yield (18.6%). The combined application of fertilizers provided better results for all the examined characteristics, while in comparison with the control, the differences ranged from 4.3% for the number of fertile spikelets to 46.5% for grain yield. The strongest correlation was determined between the spike length and the number of fertile spikelets (r = 0.939**). The obtained results lead to the conclusion that under variable environmental conditions, the application of fertilizers has a significant impact on morphological and productive characteristics of triticale, and consequently on the stability of this crop production in the organic farming system.
AB  - Cilj istraživanja bio je ispitivanje uticaja biohumusa i biofertilizatora na morfološke i produktivne osobine tritikalea u trogodišnjem periodu (2009/102011/12). Poljski ogled je postavljen kao dvofaktorijalni, po metodi blok sistema sa slučajnim rasporedom tretmana u četiri ponavljanja. Predmet ispitivanja bila je ozima sorta tritikalea, Odisej, a ispitivan je uticaj sledećih tretmana: kontrola bez đubrenja, biofertilizator (5,0 l ha-1), biohumus (3,0 t ha-1) + biofertilizator  (5,0 l ha-1). Rezultati su pokazali da spoljašnja sredina ima značajan uticaj na ekspresiju ispitivanih osobina. Najmanje vrednosti dobijene su u prvoj godini, koja je imala i najnepovoljnije meteorološke uslove. Đubrenje je imalo statistički značajan uticaj na većinu ispitivanih osobina. Primena biofertilizatora nije uticala na dužinu stabla i masu zrna u klasu, ali je značajno povećala broj plodnih klasića (3,7%), dužinu klasa (7,7%) i prinos zrna (18,6%). Kombinovanom primenom đubriva postignuti su bolji rezultati za sve ispitivane osobine, a razlike u odnosu na kontrolu bez đubrenja kretale su se u nivou od 4,3% za broj plodnih klasića do 46,5% kod prinosa zrna. Najjača korelaciona povezanost ustanovljena je između dužine klasa i broja plodnih klasića (r = 0,939**). Dobijeni rezultati upućuju na zaključak da, u promenljivim uslovima spoljašnje sredine, primena dobro izbalansiranih formula organskih i mikrobioloških đubriva ima značajan uticaj na morfološke i produktivne osobine tritikalea, a samim tim na stabilnost proizvodnje ovog useva u sistemu organskog gajenja.
PB  - Beograd : Univerzitet u Beogradu, Poljoprivredni fakultet
T2  - Journal of Agricultural Sciences
T1  - Effects of organic and microbiological fertilizers on morphological and productive characteristics of triticale in the organic farming system
T1  - Uticaj organskog i mikrobiološkog đubriva na morfološke i produktivne osobine tritikalea u sistemu organske proizvodnje
EP  - 28
IS  - 1
SP  - 13
VL  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rivec_481
ER  - 

@article{
author = "Roljević Nikolić, Svetlana and Dolijanović, Željko and Zecevic, Veselinka and Puvača, Nikola and Oljača, Snežana and Šeremešić, Srđan",
year = "2022",
abstract = "The aim of the study was to examine the impact of microbiological and organic fertilizers on morphological and productive characteristics of triticale during a three-year period (2009/10–2011/12). A two-factorial field experiment was arranged using a randomized block design with four replications. The object of the study was the triticale winter cultivar Odisej, and the following treatments were applied: a control variant without fertilization, microbiological fertilizer “Slavol” (Agrounik Serbia) 5.0 l ha-1, organic fertilizer “Biohumus Royal offert” (Altamed RS) 3.0 t ha-1 + microbiological fertilizer “Slavol” (Agrounik Serbia) 5.0 l ha-1. The results showed that the expression of the characteristics was significantly affected by the environment. The lowest values were obtained in the first year when the most unfavourable meteorological conditions were observed. The application of microbiological fertilizer had no impact on the stem length and grain weight per spike, but it significantly increased the number of fertile spikelets (3.7%), spike length (7.7%) and grain yield (18.6%). The combined application of fertilizers provided better results for all the examined characteristics, while in comparison with the control, the differences ranged from 4.3% for the number of fertile spikelets to 46.5% for grain yield. The strongest correlation was determined between the spike length and the number of fertile spikelets (r = 0.939**). The obtained results lead to the conclusion that under variable environmental conditions, the application of fertilizers has a significant impact on morphological and productive characteristics of triticale, and consequently on the stability of this crop production in the organic farming system., Cilj istraživanja bio je ispitivanje uticaja biohumusa i biofertilizatora na morfološke i produktivne osobine tritikalea u trogodišnjem periodu (2009/102011/12). Poljski ogled je postavljen kao dvofaktorijalni, po metodi blok sistema sa slučajnim rasporedom tretmana u četiri ponavljanja. Predmet ispitivanja bila je ozima sorta tritikalea, Odisej, a ispitivan je uticaj sledećih tretmana: kontrola bez đubrenja, biofertilizator (5,0 l ha-1), biohumus (3,0 t ha-1) + biofertilizator  (5,0 l ha-1). Rezultati su pokazali da spoljašnja sredina ima značajan uticaj na ekspresiju ispitivanih osobina. Najmanje vrednosti dobijene su u prvoj godini, koja je imala i najnepovoljnije meteorološke uslove. Đubrenje je imalo statistički značajan uticaj na većinu ispitivanih osobina. Primena biofertilizatora nije uticala na dužinu stabla i masu zrna u klasu, ali je značajno povećala broj plodnih klasića (3,7%), dužinu klasa (7,7%) i prinos zrna (18,6%). Kombinovanom primenom đubriva postignuti su bolji rezultati za sve ispitivane osobine, a razlike u odnosu na kontrolu bez đubrenja kretale su se u nivou od 4,3% za broj plodnih klasića do 46,5% kod prinosa zrna. Najjača korelaciona povezanost ustanovljena je između dužine klasa i broja plodnih klasića (r = 0,939**). Dobijeni rezultati upućuju na zaključak da, u promenljivim uslovima spoljašnje sredine, primena dobro izbalansiranih formula organskih i mikrobioloških đubriva ima značajan uticaj na morfološke i produktivne osobine tritikalea, a samim tim na stabilnost proizvodnje ovog useva u sistemu organskog gajenja.",
publisher = "Beograd : Univerzitet u Beogradu, Poljoprivredni fakultet",
journal = "Journal of Agricultural Sciences",
title = "Effects of organic and microbiological fertilizers on morphological and productive characteristics of triticale in the organic farming system, Uticaj organskog i mikrobiološkog đubriva na morfološke i produktivne osobine tritikalea u sistemu organske proizvodnje",
pages = "28-13",
number = "1",
volume = "67",
url = "https://hdl.handle.net/21.15107/rcub_rivec_481"
}

Roljević Nikolić, S., Dolijanović, Ž., Zecevic, V., Puvača, N., Oljača, S.,& Šeremešić, S.. (2022). Effects of organic and microbiological fertilizers on morphological and productive characteristics of triticale in the organic farming system. in Journal of Agricultural Sciences
Beograd : Univerzitet u Beogradu, Poljoprivredni fakultet., 67(1), 13-28.
https://hdl.handle.net/21.15107/rcub_rivec_481

Roljević Nikolić S, Dolijanović Ž, Zecevic V, Puvača N, Oljača S, Šeremešić S. Effects of organic and microbiological fertilizers on morphological and productive characteristics of triticale in the organic farming system. in Journal of Agricultural Sciences. 2022;67(1):13-28.
https://hdl.handle.net/21.15107/rcub_rivec_481 .

Roljević Nikolić, Svetlana, Dolijanović, Željko, Zecevic, Veselinka, Puvača, Nikola, Oljača, Snežana, Šeremešić, Srđan, "Effects of organic and microbiological fertilizers on morphological and productive characteristics of triticale in the organic farming system" in Journal of Agricultural Sciences, 67, no. 1 (2022):13-28,
https://hdl.handle.net/21.15107/rcub_rivec_481 .

TY  - JOUR
AU  - Puvača, Nikola
AU  - Tanasković, Snežana
AU  - Bursić, Vojislava
AU  - Petrović, Aleksandra
AU  - Merkuri, Jordan
AU  - Kika, Tana Shtylla
AU  - Marinković, Dušan
AU  - Vuković, Gorica
AU  - Cara, Magdalena
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5837
AB  - The aim of this research was the visual characterization and investigating the effects of Alternaria spp. contaminated wheat grains in the starter stage of broilers nutrition on productive parameters and oxidative stress. The research was divided into two phases. Bunches of wheat in post-harvest period of year 2020 was collected from a various locality in Serbia and Albania. In the first phase, collected samples were visual characterized by Alternaria spp. presence by color measurement methods. Gained results are conferred in the range of the color properties of grain color properties of Alternaria toxins. Wheat grain samples were significantly different (p < 0.05) in terms of all measured color parameters (L*, a*, b*). Classification of field fungi in analyzed wheat grain samples showed that the significant field fungi were Rhizopus spp., followed by Alternaria spp., and Fusarium spp. In the second phase, biological tests with chickens were carried out during the broiler chickens’ dietary starter period in the first 14th days of age. At the beginning of the experiment, a total of 180-day-old Ross 308 strain broilers were equally distributed into three dietary treatments, with four replicates each. Dietary treatments in the experiments were as follows: basal diet without visual contamination of Alternaria spp. with 25% wheat (A1), a basal diet with visual contamination of Alternaria spp. with 25% wheat from Serbia (A2), basal diet with visual contamination of Alternaria spp. with 25% wheat from Albania (A3). The trial with chickens lasted for 14 days. After the first experimental week, wheat infected with Alternaria spp. in treatment A2 and A3 expressed adverse effects. The highest body weight of chickens of 140.40 g was recorded in broilers on control treatment A1 with statistically significant differences (p < 0.05) compared to treatments A2 (137.32 g) and A3 (135.35 g). At the end of the second week of test period, a statistically significant (p < 0.05) difference in body weight of broiler chickens could be noticed. The highest body weight of 352.68 g was recorded in control treatment A1, with statistically significant differences compared to other Alternaria spp. treatments. The lowest body weight of chickens was recorded in treatment A3 (335.93 g). Results of feed consumption and feed conversion ratio showed some numerical differences between treatments but without any statistically significant differences (p > 0.05). Alternaria spp. contaminated diet increased glutathione (GSH), glutathione reductase (GR), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) and decreased peroxidase (POD) and superoxide dismutase (SOD) serum levels, respectively. Built on the achieved results, it can be concluded that the wheat contaminated with Alternaria spp. in broilers nutrition negatively affected growth, decreased oxidative protection and interrupted chicken welfare in the first period of life.
PB  - MDPI AG
T2  - Sustainability (Switzerland)
T1  - Optical characterization of alternaria spp. Contaminated wheat grain and its influence in early broilers nutrition on oxidative stress
IS  - 7
SP  - 4005
VL  - 13
DO  - 10.3390/su13074005
ER  - 

@article{
author = "Puvača, Nikola and Tanasković, Snežana and Bursić, Vojislava and Petrović, Aleksandra and Merkuri, Jordan and Kika, Tana Shtylla and Marinković, Dušan and Vuković, Gorica and Cara, Magdalena",
year = "2021",
abstract = "The aim of this research was the visual characterization and investigating the effects of Alternaria spp. contaminated wheat grains in the starter stage of broilers nutrition on productive parameters and oxidative stress. The research was divided into two phases. Bunches of wheat in post-harvest period of year 2020 was collected from a various locality in Serbia and Albania. In the first phase, collected samples were visual characterized by Alternaria spp. presence by color measurement methods. Gained results are conferred in the range of the color properties of grain color properties of Alternaria toxins. Wheat grain samples were significantly different (p < 0.05) in terms of all measured color parameters (L*, a*, b*). Classification of field fungi in analyzed wheat grain samples showed that the significant field fungi were Rhizopus spp., followed by Alternaria spp., and Fusarium spp. In the second phase, biological tests with chickens were carried out during the broiler chickens’ dietary starter period in the first 14th days of age. At the beginning of the experiment, a total of 180-day-old Ross 308 strain broilers were equally distributed into three dietary treatments, with four replicates each. Dietary treatments in the experiments were as follows: basal diet without visual contamination of Alternaria spp. with 25% wheat (A1), a basal diet with visual contamination of Alternaria spp. with 25% wheat from Serbia (A2), basal diet with visual contamination of Alternaria spp. with 25% wheat from Albania (A3). The trial with chickens lasted for 14 days. After the first experimental week, wheat infected with Alternaria spp. in treatment A2 and A3 expressed adverse effects. The highest body weight of chickens of 140.40 g was recorded in broilers on control treatment A1 with statistically significant differences (p < 0.05) compared to treatments A2 (137.32 g) and A3 (135.35 g). At the end of the second week of test period, a statistically significant (p < 0.05) difference in body weight of broiler chickens could be noticed. The highest body weight of 352.68 g was recorded in control treatment A1, with statistically significant differences compared to other Alternaria spp. treatments. The lowest body weight of chickens was recorded in treatment A3 (335.93 g). Results of feed consumption and feed conversion ratio showed some numerical differences between treatments but without any statistically significant differences (p > 0.05). Alternaria spp. contaminated diet increased glutathione (GSH), glutathione reductase (GR), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) and decreased peroxidase (POD) and superoxide dismutase (SOD) serum levels, respectively. Built on the achieved results, it can be concluded that the wheat contaminated with Alternaria spp. in broilers nutrition negatively affected growth, decreased oxidative protection and interrupted chicken welfare in the first period of life.",
publisher = "MDPI AG",
journal = "Sustainability (Switzerland)",
title = "Optical characterization of alternaria spp. Contaminated wheat grain and its influence in early broilers nutrition on oxidative stress",
number = "7",
pages = "4005",
volume = "13",
doi = "10.3390/su13074005"
}

Puvača, N., Tanasković, S., Bursić, V., Petrović, A., Merkuri, J., Kika, T. S., Marinković, D., Vuković, G.,& Cara, M.. (2021). Optical characterization of alternaria spp. Contaminated wheat grain and its influence in early broilers nutrition on oxidative stress. in Sustainability (Switzerland)
MDPI AG., 13(7), 4005.
https://doi.org/10.3390/su13074005

Puvača N, Tanasković S, Bursić V, Petrović A, Merkuri J, Kika TS, Marinković D, Vuković G, Cara M. Optical characterization of alternaria spp. Contaminated wheat grain and its influence in early broilers nutrition on oxidative stress. in Sustainability (Switzerland). 2021;13(7):4005.
doi:10.3390/su13074005 .

Puvača, Nikola, Tanasković, Snežana, Bursić, Vojislava, Petrović, Aleksandra, Merkuri, Jordan, Kika, Tana Shtylla, Marinković, Dušan, Vuković, Gorica, Cara, Magdalena, "Optical characterization of alternaria spp. Contaminated wheat grain and its influence in early broilers nutrition on oxidative stress" in Sustainability (Switzerland), 13, no. 7 (2021):4005,
https://doi.org/10.3390/su13074005 . .