TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Mraković, Ana
AU  - Rakočević, Zlatko
AU  - Stoiljković, Milovan
AU  - Pavlović, Vladimir B.
AU  - Momić, Tatjana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0946672X23001621
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6427
AB  - BackgroundAlthough luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro.MethodsAuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH· and ABTS·+ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay.ResultsSpherical AuNPL, stable in aqueous solution up to six months at 4 °C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH· and ABTS·+ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.ConclusionSynthesized AuNPL showed selective cytotoxic activity against HeLa cells, while being nontoxic and cytoprotective against HaCaT cells. The observed findings encourage further investigation of AuNPL as a promising novel anticancer agent.
T2  - Journal of Trace Elements in Medicine and Biology
T2  - Journal of Trace Elements in Medicine and BiologyJournal of Trace Elements in Medicine and Biology
T1  - Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach
SP  - 127286
VL  - 80
DO  - 10.1016/j.jtemb.2023.127286
ER  - 

@article{
author = "Matić, Ivana Z. and Mraković, Ana and Rakočević, Zlatko and Stoiljković, Milovan and Pavlović, Vladimir B. and Momić, Tatjana",
year = "2023",
abstract = "BackgroundAlthough luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro.MethodsAuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH· and ABTS·+ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay.ResultsSpherical AuNPL, stable in aqueous solution up to six months at 4 °C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH· and ABTS·+ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.ConclusionSynthesized AuNPL showed selective cytotoxic activity against HeLa cells, while being nontoxic and cytoprotective against HaCaT cells. The observed findings encourage further investigation of AuNPL as a promising novel anticancer agent.",
journal = "Journal of Trace Elements in Medicine and Biology, Journal of Trace Elements in Medicine and BiologyJournal of Trace Elements in Medicine and Biology",
title = "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach",
pages = "127286",
volume = "80",
doi = "10.1016/j.jtemb.2023.127286"
}

Matić, I. Z., Mraković, A., Rakočević, Z., Stoiljković, M., Pavlović, V. B.,& Momić, T.. (2023). Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology, 80, 127286.
https://doi.org/10.1016/j.jtemb.2023.127286

Matić IZ, Mraković A, Rakočević Z, Stoiljković M, Pavlović VB, Momić T. Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology. 2023;80:127286.
doi:10.1016/j.jtemb.2023.127286 .

Matić, Ivana Z., Mraković, Ana, Rakočević, Zlatko, Stoiljković, Milovan, Pavlović, Vladimir B., Momić, Tatjana, "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach" in Journal of Trace Elements in Medicine and Biology, 80 (2023):127286,
https://doi.org/10.1016/j.jtemb.2023.127286 . .

TY  - CONF
AU  - Dragičević, Vesna
AU  - Simić, Milena
AU  - Mladenović Drinić, Snežana
AU  - Stoiljković, Milovan
AU  - Dolijanović, Željko
AU  - Šenk, Milena
AU  - Brankov, Milan
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6725
AB  - Zdravlje ljudi zavisi pre svega od kvaliteta hrane, odnosno poljoprivrednih proizvoda i načina njihove proizvodnje. Ukoliko gajene biljne vrste rastu na neplodnom zemljištu ili su u većem stepenu izložene stresnim uslovima, apsorbovaće i akumulirati manje minerala, naročito esencijalnih elemenata, kao što su cink, bakar, mangan, magnezijum, kalcijum, gvožđe pa čak i sumpor, što vodi njihovom nedostatku u ishrani i povećanoj mogućnosti pojave hroničnih bolesti kod ljudi. Sa druge strane, zemljišta se iscrpljuju zahvaljujući intenzivnoj poljoprivrednoj proizvodnji, jer biljke koriste sve potrebne elemente za sopstveni razvoj i formiranje prinosa, a ne samo azot, fosfor i kalijum koji se uglavnom unose sa đubrivima. Takođe, zemljišta se mogu nalaziti na matičnoj podlozi koja je siromašna ili ne sadrži pojedine esencijalne elemente.
Zahvaljujući sinergiji sa gajenim biljkama preko korena, mikroorganizmi iz bio-đubriva podržavaju stabilniji i bolji rast i razvoj, povećavaju iskorišćenje vode i hraniva od strane biljaka, rezultirajući većim i nutritivno bogatijim prinosom. Upotreba drugih mera gajenja, kao što su plodored, združivanje useva, kao i upotreba pokrovnih useva omogućava obogaćivanje zemljišta organskom materijom. Kroz recikliranje žetvenih ostataka smanjuju se gubici hraniva, a istovremeno povećava plodnost zemljišta, kao i visina i kvalitet ostvarenih prinosa gajenih biljaka.
PB  - Akademija inženjerskih nauka Srbije, Odeljenje biotehničkih nauka i Akademska misao, Beograd
C3  - Zbornik naučnog skupa”Nove tehnologije i prakse u poljoprivredi i šumarstvu”, 24. novembar, Poljoprivredni fakultet, Beograd
T1  - Uloga održivih sistema đubrenja u povećanju kvaliteta zemljišta i poljoprivrednih proizvoda
EP  - 156
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6725
ER  - 

@conference{
author = "Dragičević, Vesna and Simić, Milena and Mladenović Drinić, Snežana and Stoiljković, Milovan and Dolijanović, Željko and Šenk, Milena and Brankov, Milan",
year = "2022",
abstract = "Zdravlje ljudi zavisi pre svega od kvaliteta hrane, odnosno poljoprivrednih proizvoda i načina njihove proizvodnje. Ukoliko gajene biljne vrste rastu na neplodnom zemljištu ili su u većem stepenu izložene stresnim uslovima, apsorbovaće i akumulirati manje minerala, naročito esencijalnih elemenata, kao što su cink, bakar, mangan, magnezijum, kalcijum, gvožđe pa čak i sumpor, što vodi njihovom nedostatku u ishrani i povećanoj mogućnosti pojave hroničnih bolesti kod ljudi. Sa druge strane, zemljišta se iscrpljuju zahvaljujući intenzivnoj poljoprivrednoj proizvodnji, jer biljke koriste sve potrebne elemente za sopstveni razvoj i formiranje prinosa, a ne samo azot, fosfor i kalijum koji se uglavnom unose sa đubrivima. Takođe, zemljišta se mogu nalaziti na matičnoj podlozi koja je siromašna ili ne sadrži pojedine esencijalne elemente.
Zahvaljujući sinergiji sa gajenim biljkama preko korena, mikroorganizmi iz bio-đubriva podržavaju stabilniji i bolji rast i razvoj, povećavaju iskorišćenje vode i hraniva od strane biljaka, rezultirajući većim i nutritivno bogatijim prinosom. Upotreba drugih mera gajenja, kao što su plodored, združivanje useva, kao i upotreba pokrovnih useva omogućava obogaćivanje zemljišta organskom materijom. Kroz recikliranje žetvenih ostataka smanjuju se gubici hraniva, a istovremeno povećava plodnost zemljišta, kao i visina i kvalitet ostvarenih prinosa gajenih biljaka.",
publisher = "Akademija inženjerskih nauka Srbije, Odeljenje biotehničkih nauka i Akademska misao, Beograd",
journal = "Zbornik naučnog skupa”Nove tehnologije i prakse u poljoprivredi i šumarstvu”, 24. novembar, Poljoprivredni fakultet, Beograd",
title = "Uloga održivih sistema đubrenja u povećanju kvaliteta zemljišta i poljoprivrednih proizvoda",
pages = "156-155",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6725"
}

Dragičević, V., Simić, M., Mladenović Drinić, S., Stoiljković, M., Dolijanović, Ž., Šenk, M.,& Brankov, M.. (2022). Uloga održivih sistema đubrenja u povećanju kvaliteta zemljišta i poljoprivrednih proizvoda. in Zbornik naučnog skupa”Nove tehnologije i prakse u poljoprivredi i šumarstvu”, 24. novembar, Poljoprivredni fakultet, Beograd
Akademija inženjerskih nauka Srbije, Odeljenje biotehničkih nauka i Akademska misao, Beograd., 155-156.
https://hdl.handle.net/21.15107/rcub_agrospace_6725

Dragičević V, Simić M, Mladenović Drinić S, Stoiljković M, Dolijanović Ž, Šenk M, Brankov M. Uloga održivih sistema đubrenja u povećanju kvaliteta zemljišta i poljoprivrednih proizvoda. in Zbornik naučnog skupa”Nove tehnologije i prakse u poljoprivredi i šumarstvu”, 24. novembar, Poljoprivredni fakultet, Beograd. 2022;:155-156.
https://hdl.handle.net/21.15107/rcub_agrospace_6725 .

Dragičević, Vesna, Simić, Milena, Mladenović Drinić, Snežana, Stoiljković, Milovan, Dolijanović, Željko, Šenk, Milena, Brankov, Milan, "Uloga održivih sistema đubrenja u povećanju kvaliteta zemljišta i poljoprivrednih proizvoda" in Zbornik naučnog skupa”Nove tehnologije i prakse u poljoprivredi i šumarstvu”, 24. novembar, Poljoprivredni fakultet, Beograd (2022):155-156,
https://hdl.handle.net/21.15107/rcub_agrospace_6725 .

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Dolijanović, Željko
AU  - Janošević, Biljana
AU  - Brankov, Milan
AU  - Stoiljković, Milovan
AU  - Dodevska, Margarita S.
AU  - Simić, Milena
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5864
AB  - Cover crops (CC) are an important low-input strategy in sustainable agricultural sys-tems. The impact of different CC (common vetch, field pea, winter oats, fodder kale, common vetch + winter oats and field pea + winter oats), organic mulch, control treatment-fallow, and bio-fertilizer (BF) application, on yield and quality of sweet maize kernel was evaluated. CC biomass was higher in mixtures: field pea + winter oats, and common vetch + winter oats, as well as in fodder kale. Kernel yield and its chemical composition varied significantly by CC, BF, year, and their interaction. Organic mulch enhanced the concentration of sugars and glutathione in maize kernel. BF increased kernel yield, the concentration of sugars, vitamin C, Mg, Fe, Zn, and reduced phytate concentration. The highest Mg and Mn concentration in maize kernel was achieved with fodder kale, Zn concentration with common vetch + winter oats + BF, and Fe concentration with winter oats. The same treatments expressed the highest impact on variability in concentration of the phytate, phenolics, and yellow pigment, thus affecting further bio-availability of essential elements. Results indicate that in a semi-arid climate, under rain-fed conditions, CC such as fodder kale and winter oats + common vetch could enhance sweet maize productivity and kernel quality, serving as an important part of a sustainable cropping system, to facilitate food security.
PB  - MDPI AG
T2  - Agronomy
T1  - Enhanced Nutritional Quality of Sweet Maize Kernel in Response to Cover Crops and Bio-Fertilizer
IS  - 5
SP  - 981
VL  - 11
DO  - 10.3390/agronomy11050981
ER  - 

@article{
author = "Dragičević, Vesna and Dolijanović, Željko and Janošević, Biljana and Brankov, Milan and Stoiljković, Milovan and Dodevska, Margarita S. and Simić, Milena",
year = "2021",
abstract = "Cover crops (CC) are an important low-input strategy in sustainable agricultural sys-tems. The impact of different CC (common vetch, field pea, winter oats, fodder kale, common vetch + winter oats and field pea + winter oats), organic mulch, control treatment-fallow, and bio-fertilizer (BF) application, on yield and quality of sweet maize kernel was evaluated. CC biomass was higher in mixtures: field pea + winter oats, and common vetch + winter oats, as well as in fodder kale. Kernel yield and its chemical composition varied significantly by CC, BF, year, and their interaction. Organic mulch enhanced the concentration of sugars and glutathione in maize kernel. BF increased kernel yield, the concentration of sugars, vitamin C, Mg, Fe, Zn, and reduced phytate concentration. The highest Mg and Mn concentration in maize kernel was achieved with fodder kale, Zn concentration with common vetch + winter oats + BF, and Fe concentration with winter oats. The same treatments expressed the highest impact on variability in concentration of the phytate, phenolics, and yellow pigment, thus affecting further bio-availability of essential elements. Results indicate that in a semi-arid climate, under rain-fed conditions, CC such as fodder kale and winter oats + common vetch could enhance sweet maize productivity and kernel quality, serving as an important part of a sustainable cropping system, to facilitate food security.",
publisher = "MDPI AG",
journal = "Agronomy",
title = "Enhanced Nutritional Quality of Sweet Maize Kernel in Response to Cover Crops and Bio-Fertilizer",
number = "5",
pages = "981",
volume = "11",
doi = "10.3390/agronomy11050981"
}

Dragičević, V., Dolijanović, Ž., Janošević, B., Brankov, M., Stoiljković, M., Dodevska, M. S.,& Simić, M.. (2021). Enhanced Nutritional Quality of Sweet Maize Kernel in Response to Cover Crops and Bio-Fertilizer. in Agronomy
MDPI AG., 11(5), 981.
https://doi.org/10.3390/agronomy11050981

Dragičević V, Dolijanović Ž, Janošević B, Brankov M, Stoiljković M, Dodevska MS, Simić M. Enhanced Nutritional Quality of Sweet Maize Kernel in Response to Cover Crops and Bio-Fertilizer. in Agronomy. 2021;11(5):981.
doi:10.3390/agronomy11050981 .

Dragičević, Vesna, Dolijanović, Željko, Janošević, Biljana, Brankov, Milan, Stoiljković, Milovan, Dodevska, Margarita S., Simić, Milena, "Enhanced Nutritional Quality of Sweet Maize Kernel in Response to Cover Crops and Bio-Fertilizer" in Agronomy, 11, no. 5 (2021):981,
https://doi.org/10.3390/agronomy11050981 . .