TY  - JOUR
AU  - Meland, Mekjell
AU  - Froynes, Oddmund
AU  - Fotirić-Akšić, Milica
AU  - Pojskić, Naris
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Gasi, Fuad
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5335
AB  - European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers
IS  - 2
VL  - 10
DO  - 10.3390/agronomy10020264
ER  - 

@article{
author = "Meland, Mekjell and Froynes, Oddmund and Fotirić-Akšić, Milica and Pojskić, Naris and Kalamujić-Stroil, Belma and Lasić, Lejla and Gasi, Fuad",
year = "2020",
abstract = "European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers",
number = "2",
volume = "10",
doi = "10.3390/agronomy10020264"
}

Meland, M., Froynes, O., Fotirić-Akšić, M., Pojskić, N., Kalamujić-Stroil, B., Lasić, L.,& Gasi, F.. (2020). Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel
MDPI, BASEL., 10(2).
https://doi.org/10.3390/agronomy10020264

Meland M, Froynes O, Fotirić-Akšić M, Pojskić N, Kalamujić-Stroil B, Lasić L, Gasi F. Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel. 2020;10(2).
doi:10.3390/agronomy10020264 .

Meland, Mekjell, Froynes, Oddmund, Fotirić-Akšić, Milica, Pojskić, Naris, Kalamujić-Stroil, Belma, Lasić, Lejla, Gasi, Fuad, "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers" in Agronomy-Basel, 10, no. 2 (2020),
https://doi.org/10.3390/agronomy10020264 . .

TY  - JOUR
AU  - Gasi, Fuad
AU  - Froynes, Oddmund
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Pojskić, Naris
AU  - Fotirić-Akšić, Milica
AU  - Meland, Mekjell
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5291
AB  - The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'
IS  - 9
VL  - 10
DO  - 10.3390/agronomy10091372
ER  - 

@article{
author = "Gasi, Fuad and Froynes, Oddmund and Kalamujić-Stroil, Belma and Lasić, Lejla and Pojskić, Naris and Fotirić-Akšić, Milica and Meland, Mekjell",
year = "2020",
abstract = "The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'",
number = "9",
volume = "10",
doi = "10.3390/agronomy10091372"
}

Gasi, F., Froynes, O., Kalamujić-Stroil, B., Lasić, L., Pojskić, N., Fotirić-Akšić, M.,& Meland, M.. (2020). S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel
MDPI, BASEL., 10(9).
https://doi.org/10.3390/agronomy10091372

Gasi F, Froynes O, Kalamujić-Stroil B, Lasić L, Pojskić N, Fotirić-Akšić M, Meland M. S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel. 2020;10(9).
doi:10.3390/agronomy10091372 .

Gasi, Fuad, Froynes, Oddmund, Kalamujić-Stroil, Belma, Lasić, Lejla, Pojskić, Naris, Fotirić-Akšić, Milica, Meland, Mekjell, "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'" in Agronomy-Basel, 10, no. 9 (2020),
https://doi.org/10.3390/agronomy10091372 . .

TY  - JOUR
AU  - Gaši, Fuad
AU  - Žulj-Mihaljević, Maja
AU  - Šimon, Silvio
AU  - Grahić, Jasmin
AU  - Pojskić, Naris
AU  - Kurtović, Mirsad
AU  - Nikolić, Dragan
AU  - Pejić, Ivan
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3098
AB  - In order to identify possible mislabeling of the apple accession maintained ex situ in Srebrenik and to gain insight into the genetic structure of the conserved germplasm, 14 accessions from the collection were genotyped using 10 SSR (Simple Sequence Repeats) markers. Obtained SSR profiles were then added to an existing database constructed for previously characterized 24 traditional and 13 international, reference apple cultivars maintained at the same collection. Bayesian analysis implemented in the STRUCTURE program grouped 42 out of 51 analyzed apple accessions (38 traditional and 13 international) into three RPPs (reconstructed panmictic populations) with probability of membership qI higher than 75%. Almost all international, reference cultivars grouped in RPP3, whereas traditional B&H cultivars from the Srebrenik collection grouped in all three RPPs. Large and significant differentiations between all three individual RPPs were detected through the analyses of molecular variance and confirmed with FCA (factorial correspondence analyses). NJ cluster analysis, based on the Bruvo genetic distance, revealed that out of 38 traditional B&H apple cultivars, analyzed in the study, 'Ljepocvjetka', 'Bobovec' and 'Bobovec J' grouped closest to the international reference cultivars. Available date indicates that unlike a large number of B&H apple cultivars which were introduced during the reign of the Ottoman Empire, 'Ljepocvjetka' and 'Bobovec' were probably introduced at a later date. Cluster analyses also enabled the detection of one synonym and three homonyms within the collection. In four cases, previously conducted identification based on phenotypic analyses was confirmed by genetic analyses. Results of the structure analyses indicate a heterogeneous genetic structure of the analyzed accessions. This characteristic of the B&H apple germplasm could be useful for future breeding programs.
AB  - U cilju sticanja uvida u genetsku strukturu germplazme jabuke održavane ex situ u Srebreniku, 14 aksešna iz date kolekcije genotipizirano je korišćenjem 10 SSR markera. Dobijeni SSR profili dodati su već postojećoj bazi podataka sačinjenoj od prethodno karakteriziranih 24 tradicionalnih i 13 internacionalnih, referentnih sorti koji se održavaju u istoj kolekciji. Bayesian analiza sprovedena u STUCTURE računarskom programu grupisala je 42 od 51 analiziranog aksešna jabuke (38 tradicionalnih i 13 internacionalnih) u tri RPP-a (rekonstruisane panmiktičke populacije) sa verovatnoćom članstva ql veće od 75%. Gotovo sve internacionalne, referentne sorte grupisale su se u RPP3, dok se tradicionalne BiH sorte iz Srebrenika mogu naći u sva tri RPP-a. Visoka i statistički značajna diferencijacija uočena je između pojedinačnih RPP-ova i dodatno potvrđena sa FCA (faktorska korespondentna analiza). NJ klaster analiza, bazirana na Bruvo genetskoj udaljenosti, otkrila je da od 38 ispitivanih tradicionalnih BiH sorti jabuke, 'Ljepocvjetka', 'Bobovec' i 'Bobovec J.' se grupišu najbliže internacionalnim, referentnim sortama. Dostupni podaci ukazuju da za razliku od velikog broja BiH sorti jabuke koje su introdukovane tokom vladavine Osmanskog carstva, 'Ljepocvjetka' i 'Bobovec' su verovatno introdukovane na ove prostore nešto kasnije. Klaster analiza je takođe omogućila detekciju jednog sinonima i tri homonima unutar kolekcije. U četiri slučaja, prethodno sprovedena identifikacija na osnovu fenotipa je potvrđena genetskim analizama. Rezultati analize genetske strukture ukazuju na heterogenu strukturu analiziranih aksešna. Ova karakteristika BiH germplazme jabuke može biti interesantna za buduće oplemenjivačke programe.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers
T1  - Genetska struktura aksešna jabuke održavanih ex situ u Bosni i Herzegovini ispitana mikrosatelitskim markerima
EP  - 478
IS  - 2
SP  - 467
VL  - 45
DO  - 10.2298/GENSR1302467G
ER  - 

@article{
author = "Gaši, Fuad and Žulj-Mihaljević, Maja and Šimon, Silvio and Grahić, Jasmin and Pojskić, Naris and Kurtović, Mirsad and Nikolić, Dragan and Pejić, Ivan",
year = "2013",
abstract = "In order to identify possible mislabeling of the apple accession maintained ex situ in Srebrenik and to gain insight into the genetic structure of the conserved germplasm, 14 accessions from the collection were genotyped using 10 SSR (Simple Sequence Repeats) markers. Obtained SSR profiles were then added to an existing database constructed for previously characterized 24 traditional and 13 international, reference apple cultivars maintained at the same collection. Bayesian analysis implemented in the STRUCTURE program grouped 42 out of 51 analyzed apple accessions (38 traditional and 13 international) into three RPPs (reconstructed panmictic populations) with probability of membership qI higher than 75%. Almost all international, reference cultivars grouped in RPP3, whereas traditional B&H cultivars from the Srebrenik collection grouped in all three RPPs. Large and significant differentiations between all three individual RPPs were detected through the analyses of molecular variance and confirmed with FCA (factorial correspondence analyses). NJ cluster analysis, based on the Bruvo genetic distance, revealed that out of 38 traditional B&H apple cultivars, analyzed in the study, 'Ljepocvjetka', 'Bobovec' and 'Bobovec J' grouped closest to the international reference cultivars. Available date indicates that unlike a large number of B&H apple cultivars which were introduced during the reign of the Ottoman Empire, 'Ljepocvjetka' and 'Bobovec' were probably introduced at a later date. Cluster analyses also enabled the detection of one synonym and three homonyms within the collection. In four cases, previously conducted identification based on phenotypic analyses was confirmed by genetic analyses. Results of the structure analyses indicate a heterogeneous genetic structure of the analyzed accessions. This characteristic of the B&H apple germplasm could be useful for future breeding programs., U cilju sticanja uvida u genetsku strukturu germplazme jabuke održavane ex situ u Srebreniku, 14 aksešna iz date kolekcije genotipizirano je korišćenjem 10 SSR markera. Dobijeni SSR profili dodati su već postojećoj bazi podataka sačinjenoj od prethodno karakteriziranih 24 tradicionalnih i 13 internacionalnih, referentnih sorti koji se održavaju u istoj kolekciji. Bayesian analiza sprovedena u STUCTURE računarskom programu grupisala je 42 od 51 analiziranog aksešna jabuke (38 tradicionalnih i 13 internacionalnih) u tri RPP-a (rekonstruisane panmiktičke populacije) sa verovatnoćom članstva ql veće od 75%. Gotovo sve internacionalne, referentne sorte grupisale su se u RPP3, dok se tradicionalne BiH sorte iz Srebrenika mogu naći u sva tri RPP-a. Visoka i statistički značajna diferencijacija uočena je između pojedinačnih RPP-ova i dodatno potvrđena sa FCA (faktorska korespondentna analiza). NJ klaster analiza, bazirana na Bruvo genetskoj udaljenosti, otkrila je da od 38 ispitivanih tradicionalnih BiH sorti jabuke, 'Ljepocvjetka', 'Bobovec' i 'Bobovec J.' se grupišu najbliže internacionalnim, referentnim sortama. Dostupni podaci ukazuju da za razliku od velikog broja BiH sorti jabuke koje su introdukovane tokom vladavine Osmanskog carstva, 'Ljepocvjetka' i 'Bobovec' su verovatno introdukovane na ove prostore nešto kasnije. Klaster analiza je takođe omogućila detekciju jednog sinonima i tri homonima unutar kolekcije. U četiri slučaja, prethodno sprovedena identifikacija na osnovu fenotipa je potvrđena genetskim analizama. Rezultati analize genetske strukture ukazuju na heterogenu strukturu analiziranih aksešna. Ova karakteristika BiH germplazme jabuke može biti interesantna za buduće oplemenjivačke programe.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers, Genetska struktura aksešna jabuke održavanih ex situ u Bosni i Herzegovini ispitana mikrosatelitskim markerima",
pages = "478-467",
number = "2",
volume = "45",
doi = "10.2298/GENSR1302467G"
}

Gaši, F., Žulj-Mihaljević, M., Šimon, S., Grahić, J., Pojskić, N., Kurtović, M., Nikolić, D.,& Pejić, I.. (2013). Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers. in Genetika
Društvo genetičara Srbije, Beograd., 45(2), 467-478.
https://doi.org/10.2298/GENSR1302467G

Gaši F, Žulj-Mihaljević M, Šimon S, Grahić J, Pojskić N, Kurtović M, Nikolić D, Pejić I. Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers. in Genetika. 2013;45(2):467-478.
doi:10.2298/GENSR1302467G .

Gaši, Fuad, Žulj-Mihaljević, Maja, Šimon, Silvio, Grahić, Jasmin, Pojskić, Naris, Kurtović, Mirsad, Nikolić, Dragan, Pejić, Ivan, "Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers" in Genetika, 45, no. 2 (2013):467-478,
https://doi.org/10.2298/GENSR1302467G . .