TY  - JOUR
AU  - Pruesse, Ulf
AU  - Bilancetti, Luca
AU  - Bucko, Marek
AU  - Bugarski, Branko
AU  - Bukowski, Jozef
AU  - Gemeiner, Peter
AU  - Lewinska, Dorota
AU  - Manojlović, Verica
AU  - Massart, Benjamin
AU  - Nastruzzi, Claudio
AU  - Nedović, Viktor
AU  - Poncelet, Denis
AU  - Siebenhaar, Swen
AU  - Tobler, Lucien
AU  - Tosi, Azzurra
AU  - Vikartovska, Alica
AU  - Vorlop, Klaus-Dieter
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1813
AB  - This paper describes the results of the round robin experiment "Bead production technologies" carried out during the COST 840 action "Bioencapsulation Innovation and Technologies" within the 5th Framework Program of the European Community. In this round robin experiment, calcium alginate hydrogel beads with the diameter of (800 +/- 100) mu m were produced by the most common bead production technologies using 0.5-4 mass % sodium alginate solutions as starting material. Dynamic viscosity of the alginate solutions ranged from less than 50 mPa s up to more than 10000 mPa s. With the coaxial air-flow and electrostatic enhanced dropping technologies as well as with the JetCutter technology in the soft-landing mode, beads were produced from all alginate solutions, whereas the vibration technology was not capable to process the high-viscosity 3 % and 4 % alginate solutions. Spherical beads were generated by the electrostatic and the JetCutter technologies. Slightly deformed beads were obtained from high-viscosity alginate solutions using the coaxial airflow and from the 0.5 % and 2 % alginate solutions using the vibration technology. The rate of bead production using the JetCutter was about 10 times higher than with the vibration technology and more than 10000 times higher than with the coaxial air-flow and electrostatic technology.
PB  - Springer International Publishing Ag, Cham
T2  - Chemical Papers
T1  - Comparison of different technologies for alginate beads production
EP  - 374
IS  - 4
SP  - 364
VL  - 62
DO  - 10.2478/s11696-008-0035-x
ER  - 

@article{
author = "Pruesse, Ulf and Bilancetti, Luca and Bucko, Marek and Bugarski, Branko and Bukowski, Jozef and Gemeiner, Peter and Lewinska, Dorota and Manojlović, Verica and Massart, Benjamin and Nastruzzi, Claudio and Nedović, Viktor and Poncelet, Denis and Siebenhaar, Swen and Tobler, Lucien and Tosi, Azzurra and Vikartovska, Alica and Vorlop, Klaus-Dieter",
year = "2008",
abstract = "This paper describes the results of the round robin experiment "Bead production technologies" carried out during the COST 840 action "Bioencapsulation Innovation and Technologies" within the 5th Framework Program of the European Community. In this round robin experiment, calcium alginate hydrogel beads with the diameter of (800 +/- 100) mu m were produced by the most common bead production technologies using 0.5-4 mass % sodium alginate solutions as starting material. Dynamic viscosity of the alginate solutions ranged from less than 50 mPa s up to more than 10000 mPa s. With the coaxial air-flow and electrostatic enhanced dropping technologies as well as with the JetCutter technology in the soft-landing mode, beads were produced from all alginate solutions, whereas the vibration technology was not capable to process the high-viscosity 3 % and 4 % alginate solutions. Spherical beads were generated by the electrostatic and the JetCutter technologies. Slightly deformed beads were obtained from high-viscosity alginate solutions using the coaxial airflow and from the 0.5 % and 2 % alginate solutions using the vibration technology. The rate of bead production using the JetCutter was about 10 times higher than with the vibration technology and more than 10000 times higher than with the coaxial air-flow and electrostatic technology.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Chemical Papers",
title = "Comparison of different technologies for alginate beads production",
pages = "374-364",
number = "4",
volume = "62",
doi = "10.2478/s11696-008-0035-x"
}

Pruesse, U., Bilancetti, L., Bucko, M., Bugarski, B., Bukowski, J., Gemeiner, P., Lewinska, D., Manojlović, V., Massart, B., Nastruzzi, C., Nedović, V., Poncelet, D., Siebenhaar, S., Tobler, L., Tosi, A., Vikartovska, A.,& Vorlop, K.. (2008). Comparison of different technologies for alginate beads production. in Chemical Papers
Springer International Publishing Ag, Cham., 62(4), 364-374.
https://doi.org/10.2478/s11696-008-0035-x

Pruesse U, Bilancetti L, Bucko M, Bugarski B, Bukowski J, Gemeiner P, Lewinska D, Manojlović V, Massart B, Nastruzzi C, Nedović V, Poncelet D, Siebenhaar S, Tobler L, Tosi A, Vikartovska A, Vorlop K. Comparison of different technologies for alginate beads production. in Chemical Papers. 2008;62(4):364-374.
doi:10.2478/s11696-008-0035-x .

Pruesse, Ulf, Bilancetti, Luca, Bucko, Marek, Bugarski, Branko, Bukowski, Jozef, Gemeiner, Peter, Lewinska, Dorota, Manojlović, Verica, Massart, Benjamin, Nastruzzi, Claudio, Nedović, Viktor, Poncelet, Denis, Siebenhaar, Swen, Tobler, Lucien, Tosi, Azzurra, Vikartovska, Alica, Vorlop, Klaus-Dieter, "Comparison of different technologies for alginate beads production" in Chemical Papers, 62, no. 4 (2008):364-374,
https://doi.org/10.2478/s11696-008-0035-x . .

TY  - CONF
AU  - Nedović, Viktor
AU  - Obradović, B
AU  - Poncelet, Denis
AU  - Goosen, MFA
AU  - Leskošek-Čukalović, Ida
AU  - Bugarski, Branko
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/506
AB  - This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.
PB  - Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig
C3  - Practical Aspects of Encapsulation Technologies
T1  - Cell immobilisation by electrostatic droplet generation
EP  - 17
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_506
ER  - 

@conference{
author = "Nedović, Viktor and Obradović, B and Poncelet, Denis and Goosen, MFA and Leskošek-Čukalović, Ida and Bugarski, Branko",
year = "2002",
abstract = "This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.",
publisher = "Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig",
journal = "Practical Aspects of Encapsulation Technologies",
title = "Cell immobilisation by electrostatic droplet generation",
pages = "17-11",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_506"
}

Nedović, V., Obradović, B., Poncelet, D., Goosen, M., Leskošek-Čukalović, I.,& Bugarski, B.. (2002). Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies
Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig., 11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506

Nedović V, Obradović B, Poncelet D, Goosen M, Leskošek-Čukalović I, Bugarski B. Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies. 2002;:11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506 .

Nedović, Viktor, Obradović, B, Poncelet, Denis, Goosen, MFA, Leskošek-Čukalović, Ida, Bugarski, Branko, "Cell immobilisation by electrostatic droplet generation" in Practical Aspects of Encapsulation Technologies (2002):11-17,
https://hdl.handle.net/21.15107/rcub_agrospace_506 .