TY  - JOUR
AU  - Madzovska-Malagurski, I.
AU  - Vukašinović-Sekulić, M.
AU  - Kostić, D.
AU  - Lević, Steva
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4181
AB  - The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.
PB  - IOP Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Towards antimicrobial yet bioactive Cu-alginate hydrogels
IS  - 3
VL  - 11
DO  - 10.1088/1748-6041/11/3/035015
ER  - 

@article{
author = "Madzovska-Malagurski, I. and Vukašinović-Sekulić, M. and Kostić, D. and Lević, Steva",
year = "2016",
abstract = "The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Towards antimicrobial yet bioactive Cu-alginate hydrogels",
number = "3",
volume = "11",
doi = "10.1088/1748-6041/11/3/035015"
}

Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D.,& Lević, S.. (2016). Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials
IOP Publishing Ltd, Bristol., 11(3).
https://doi.org/10.1088/1748-6041/11/3/035015

Madzovska-Malagurski I, Vukašinović-Sekulić M, Kostić D, Lević S. Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials. 2016;11(3).
doi:10.1088/1748-6041/11/3/035015 .

Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D., Lević, Steva, "Towards antimicrobial yet bioactive Cu-alginate hydrogels" in Biomedical Materials, 11, no. 3 (2016),
https://doi.org/10.1088/1748-6041/11/3/035015 . .