TY  - JOUR
AU  - Vasilijević, Bojana
AU  - Mitić-Culafić, Dragana
AU  - Đekić, Ilija
AU  - Marković, Tatjana
AU  - Knežević-Vukčević, Jelena
AU  - Tomašević, Igor
AU  - Velebit, Branko
AU  - Nikolić, Biljana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5112
AB  - Juniperus communis and Satureja montana essential oils (EOs) were chemically characterized and their activity against Listeria monocytogenes was evaluated. Antilisterial effect was determined in vitro and in situ, in red winemarinated beef, where the effect against common meat contaminants (aerobic heterotrophic mesophyll bacteria, Enterobacteriaceae and lactic acid bacteria) was also monitored. Microdilution assay on L. monocytogenes (strain ATCC19111 and three primoisolates) showed that minimal inhibitory concentrations (MICs) varied in the range 0.5-1% for both EOs. Effect of EOs mixtures was monitored in checkerboard assay revealing that all types of interactions including synergism were obtained only for isolates. In vitro time kill assay confirmed the mode of interactions: synergism for isolate from beef carcass (LMB), and its absence for ATCC19111 strain. The analysis of concentration dependent growth rates showed that estimated curve MIC values were notably lower than in microdilution assay (0.03-0.14%). In situ time kill assay against inoculated L. monocytogenes (ATCC19111 and LMB strains) and pre-existing meat spoiling contaminants was performed on marinated beef using EOs concentrations that were confirmed to be sensory acceptable. Basic red wine marinades and the ones containing each EO or their combination remarkably decreased the counts of all monitored groups comparing to saline control. The most pronounced effect was obtained with the marinade containing EOs mixture. For all monitored bacteria, the bactericidal effect during marination was followed by bacteriostatic effect during subsequent meat storage. Our results demonstrate for the first time the in situ efficacy of J. communis EO against meat contaminants and provide additional data on the preservation potential of S. montana EO.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Antibacterial effect of Juniperus communis and Satureja montana essential oils against Listeria monocytogenes in vitro and in wine marinated beef
EP  - 256
SP  - 247
VL  - 100
DO  - 10.1016/j.foodcont.2019.01.025
ER  - 

@article{
author = "Vasilijević, Bojana and Mitić-Culafić, Dragana and Đekić, Ilija and Marković, Tatjana and Knežević-Vukčević, Jelena and Tomašević, Igor and Velebit, Branko and Nikolić, Biljana",
year = "2019",
abstract = "Juniperus communis and Satureja montana essential oils (EOs) were chemically characterized and their activity against Listeria monocytogenes was evaluated. Antilisterial effect was determined in vitro and in situ, in red winemarinated beef, where the effect against common meat contaminants (aerobic heterotrophic mesophyll bacteria, Enterobacteriaceae and lactic acid bacteria) was also monitored. Microdilution assay on L. monocytogenes (strain ATCC19111 and three primoisolates) showed that minimal inhibitory concentrations (MICs) varied in the range 0.5-1% for both EOs. Effect of EOs mixtures was monitored in checkerboard assay revealing that all types of interactions including synergism were obtained only for isolates. In vitro time kill assay confirmed the mode of interactions: synergism for isolate from beef carcass (LMB), and its absence for ATCC19111 strain. The analysis of concentration dependent growth rates showed that estimated curve MIC values were notably lower than in microdilution assay (0.03-0.14%). In situ time kill assay against inoculated L. monocytogenes (ATCC19111 and LMB strains) and pre-existing meat spoiling contaminants was performed on marinated beef using EOs concentrations that were confirmed to be sensory acceptable. Basic red wine marinades and the ones containing each EO or their combination remarkably decreased the counts of all monitored groups comparing to saline control. The most pronounced effect was obtained with the marinade containing EOs mixture. For all monitored bacteria, the bactericidal effect during marination was followed by bacteriostatic effect during subsequent meat storage. Our results demonstrate for the first time the in situ efficacy of J. communis EO against meat contaminants and provide additional data on the preservation potential of S. montana EO.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Antibacterial effect of Juniperus communis and Satureja montana essential oils against Listeria monocytogenes in vitro and in wine marinated beef",
pages = "256-247",
volume = "100",
doi = "10.1016/j.foodcont.2019.01.025"
}

Vasilijević, B., Mitić-Culafić, D., Đekić, I., Marković, T., Knežević-Vukčević, J., Tomašević, I., Velebit, B.,& Nikolić, B.. (2019). Antibacterial effect of Juniperus communis and Satureja montana essential oils against Listeria monocytogenes in vitro and in wine marinated beef. in Food Control
Elsevier Sci Ltd, Oxford., 100, 247-256.
https://doi.org/10.1016/j.foodcont.2019.01.025

Vasilijević B, Mitić-Culafić D, Đekić I, Marković T, Knežević-Vukčević J, Tomašević I, Velebit B, Nikolić B. Antibacterial effect of Juniperus communis and Satureja montana essential oils against Listeria monocytogenes in vitro and in wine marinated beef. in Food Control. 2019;100:247-256.
doi:10.1016/j.foodcont.2019.01.025 .

Vasilijević, Bojana, Mitić-Culafić, Dragana, Đekić, Ilija, Marković, Tatjana, Knežević-Vukčević, Jelena, Tomašević, Igor, Velebit, Branko, Nikolić, Biljana, "Antibacterial effect of Juniperus communis and Satureja montana essential oils against Listeria monocytogenes in vitro and in wine marinated beef" in Food Control, 100 (2019):247-256,
https://doi.org/10.1016/j.foodcont.2019.01.025 . .

TY  - JOUR
AU  - Bosnjak-Neumuller, Jasna
AU  - Djelić, Ninoslav
AU  - Radaković, Milena
AU  - Kolarević, Stoimir
AU  - Mitić-Culafić, Dragana
AU  - Dajić-Stevanović, Zora
AU  - Vuković-Gačić, Branka
AU  - Knežević-Vukčević, Jelena
AU  - Stanimirović, Zoran
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4409
AB  - There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro
EP  - 397
IS  - 2
SP  - 387
VL  - 49
DO  - 10.2298/GENSR1702387B
ER  - 

@article{
author = "Bosnjak-Neumuller, Jasna and Djelić, Ninoslav and Radaković, Milena and Kolarević, Stoimir and Mitić-Culafić, Dragana and Dajić-Stevanović, Zora and Vuković-Gačić, Branka and Knežević-Vukčević, Jelena and Stanimirović, Zoran",
year = "2017",
abstract = "There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro",
pages = "397-387",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702387B"
}

Bosnjak-Neumuller, J., Djelić, N., Radaković, M., Kolarević, S., Mitić-Culafić, D., Dajić-Stevanović, Z., Vuković-Gačić, B., Knežević-Vukčević, J.,& Stanimirović, Z.. (2017). Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 387-397.
https://doi.org/10.2298/GENSR1702387B

Bosnjak-Neumuller J, Djelić N, Radaković M, Kolarević S, Mitić-Culafić D, Dajić-Stevanović Z, Vuković-Gačić B, Knežević-Vukčević J, Stanimirović Z. Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro. in Genetika. 2017;49(2):387-397.
doi:10.2298/GENSR1702387B .

Bosnjak-Neumuller, Jasna, Djelić, Ninoslav, Radaković, Milena, Kolarević, Stoimir, Mitić-Culafić, Dragana, Dajić-Stevanović, Zora, Vuković-Gačić, Branka, Knežević-Vukčević, Jelena, Stanimirović, Zoran, "Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro" in Genetika, 49, no. 2 (2017):387-397,
https://doi.org/10.2298/GENSR1702387B . .