TY  - CONF
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Lazić, Vesna
AU  - Spirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Spasić, Snežana
AU  - Jakovljević, Dragica
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6550
AB  - Excessive production of reactive oxygen species (ROS) often results in irreversible cell
damage and leads to a number of disorders such as hypertension, heart disease, diabetes,
inflammation, premature aging and cancer. Considering the importance of diet in prevention
of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant
activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural
source of functional food ingredients. Nowadays functional food products based on the
lignicolous mushroom species are increasingly available on the market. Their powders and
extracts are consumed as dietary supplements in the form of capsules or tablets and as
additives in the formulation of healthier food products.
Antioxidant capacity of F. pinicola methanol extract was evaluated in vitro by the inhibition
of lipid peroxidation (LPx) in the linoleic acid model system. Results were normalized and
expressed as EC50 values (μg/mL). The Folin-Ciocalteu reaction adapted for a 96-well
microplate reader was used to determine the total phenol content (TPC) and result was
expressed as mg of gallic acid equivalent (GAE) per g of dry weight (DW) of extract.
Phenolic profile of the extract was analyzed by the liquid chromatography coupled with
tandem mass spectrometry (LC-MS/MS). Based on the analysis, TPC of the methanol extract
was reported to be 133.11 mg GAE/g of DW. Among hydroxybenzoic acids, the most
abundant was gallic acid, represented by 951.12 μg/g of DW. Also, presence of ellagic,
protocatechuic, p-hydroxybenzoic and syringic acid was observed. Among hydroxycinnamic
acids, chlorogenic acid was the most prevalent, with 0.76 μg/g of DW. With regard to
antioxidant activity and inhibition of LPx, EC50 value for F. pinicola methanol extract was
20.0 μg/mL.
The results of the present study suggest that methanol extract of the wild lignicolus
mushroom F. pinicola acts as natural antioxidant in the prevention of LPx. This mushroom
may be a good source for the development of antioxidant additives.
PB  - МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA
C3  - The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses
T1  - Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6550
ER  - 

@conference{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Lazić, Vesna and Spirović Trifunović, Bojana and Miletić, Srđan and Spasić, Snežana and Jakovljević, Dragica",
year = "2022",
abstract = "Excessive production of reactive oxygen species (ROS) often results in irreversible cell
damage and leads to a number of disorders such as hypertension, heart disease, diabetes,
inflammation, premature aging and cancer. Considering the importance of diet in prevention
of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant
activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural
source of functional food ingredients. Nowadays functional food products based on the
lignicolous mushroom species are increasingly available on the market. Their powders and
extracts are consumed as dietary supplements in the form of capsules or tablets and as
additives in the formulation of healthier food products.
Antioxidant capacity of F. pinicola methanol extract was evaluated in vitro by the inhibition
of lipid peroxidation (LPx) in the linoleic acid model system. Results were normalized and
expressed as EC50 values (μg/mL). The Folin-Ciocalteu reaction adapted for a 96-well
microplate reader was used to determine the total phenol content (TPC) and result was
expressed as mg of gallic acid equivalent (GAE) per g of dry weight (DW) of extract.
Phenolic profile of the extract was analyzed by the liquid chromatography coupled with
tandem mass spectrometry (LC-MS/MS). Based on the analysis, TPC of the methanol extract
was reported to be 133.11 mg GAE/g of DW. Among hydroxybenzoic acids, the most
abundant was gallic acid, represented by 951.12 μg/g of DW. Also, presence of ellagic,
protocatechuic, p-hydroxybenzoic and syringic acid was observed. Among hydroxycinnamic
acids, chlorogenic acid was the most prevalent, with 0.76 μg/g of DW. With regard to
antioxidant activity and inhibition of LPx, EC50 value for F. pinicola methanol extract was
20.0 μg/mL.
The results of the present study suggest that methanol extract of the wild lignicolus
mushroom F. pinicola acts as natural antioxidant in the prevention of LPx. This mushroom
may be a good source for the development of antioxidant additives.",
publisher = "МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA",
journal = "The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses",
title = "Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6550"
}

Kozarski, M., Klaus, A., Vunduk, J., Lazić, V., Spirović Trifunović, B., Miletić, S., Spasić, S.,& Jakovljević, D.. (2022). Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation. in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses
МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA..
https://hdl.handle.net/21.15107/rcub_agrospace_6550

Kozarski M, Klaus A, Vunduk J, Lazić V, Spirović Trifunović B, Miletić S, Spasić S, Jakovljević D. Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation. in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6550 .

Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Lazić, Vesna, Spirović Trifunović, Bojana, Miletić, Srđan, Spasić, Snežana, Jakovljević, Dragica, "Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation" in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6550 .

TY  - JOUR
AU  - Udovički, Božidar
AU  - Tomić, Nikola
AU  - Spirović Trifunović, Bojana
AU  - Despotović, Saša
AU  - Jovanović, Jelena
AU  - Jacxsens, Liesbeth
AU  - Rajković, Andreja
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5826
AB  - Classified as Group 1 carcinogens aflatoxins (AFs) are of major concern for food safety and public health. Within the AFs group, aflatoxin B1 (AFB1) occurs at the highest levels in different food products and is considered as the most potent representative. A total of 463 samples of products susceptible to contamination with AFB1 and commonly consumed in Serbia were collected and analyzed. Consumption surveys were performed using a food frequency questionnaire (FFQ) and 24-h recall-based method. Monte Carlo simulation of 100,000 iterations was performed to estimate the intake of AFB1 through each food category, while a simple distribution approach was performed to estimate overall exposure of tested population groups. Risk characterization was performed by calculation of the Margin of Exposure (MOE) and by calculation of the number of possible hepatocellular carcinoma (HCC) cases. The highest exposure was observed in children with the mean overall estimated daily intake (EDI) in the range of 0.79–1.10 and 1.20–1.66 ng kg−1 bw day−1, followed by adolescents 0.56–0.81 and 0.94–1.32 ng kg−1 bw day−1, adult females 0.52–0.72 and 0.56–0.76 ng kg−1 bw day−1 and adult males with the mean overall EDI in the range of 0.39–0.56 and 0.47–0.66 ng kg−1 bw day−1 depending on consumption survey method and for the lower (LB) and upper bound (UB) scenario. MOE values that resulted from the mean values of the overall EDI of AFB1 were low, indicating high concern for all population groups. Based on obtained mean values of the overall EDI higher estimates of possible HCC cases caused by exposure to AFB1 were in the range of 0.01–0.02 cases/year/105 individuals, depending on the population group.
PB  - Elsevier Ltd
T2  - Food and Chemical Toxicology
T1  - Risk assessment of dietary exposure to aflatoxin B1 in Serbia
SP  - 112116
VL  - 151
DO  - 10.1016/j.fct.2021.112116
ER  - 

@article{
author = "Udovički, Božidar and Tomić, Nikola and Spirović Trifunović, Bojana and Despotović, Saša and Jovanović, Jelena and Jacxsens, Liesbeth and Rajković, Andreja",
year = "2021",
abstract = "Classified as Group 1 carcinogens aflatoxins (AFs) are of major concern for food safety and public health. Within the AFs group, aflatoxin B1 (AFB1) occurs at the highest levels in different food products and is considered as the most potent representative. A total of 463 samples of products susceptible to contamination with AFB1 and commonly consumed in Serbia were collected and analyzed. Consumption surveys were performed using a food frequency questionnaire (FFQ) and 24-h recall-based method. Monte Carlo simulation of 100,000 iterations was performed to estimate the intake of AFB1 through each food category, while a simple distribution approach was performed to estimate overall exposure of tested population groups. Risk characterization was performed by calculation of the Margin of Exposure (MOE) and by calculation of the number of possible hepatocellular carcinoma (HCC) cases. The highest exposure was observed in children with the mean overall estimated daily intake (EDI) in the range of 0.79–1.10 and 1.20–1.66 ng kg−1 bw day−1, followed by adolescents 0.56–0.81 and 0.94–1.32 ng kg−1 bw day−1, adult females 0.52–0.72 and 0.56–0.76 ng kg−1 bw day−1 and adult males with the mean overall EDI in the range of 0.39–0.56 and 0.47–0.66 ng kg−1 bw day−1 depending on consumption survey method and for the lower (LB) and upper bound (UB) scenario. MOE values that resulted from the mean values of the overall EDI of AFB1 were low, indicating high concern for all population groups. Based on obtained mean values of the overall EDI higher estimates of possible HCC cases caused by exposure to AFB1 were in the range of 0.01–0.02 cases/year/105 individuals, depending on the population group.",
publisher = "Elsevier Ltd",
journal = "Food and Chemical Toxicology",
title = "Risk assessment of dietary exposure to aflatoxin B1 in Serbia",
pages = "112116",
volume = "151",
doi = "10.1016/j.fct.2021.112116"
}

Udovički, B., Tomić, N., Spirović Trifunović, B., Despotović, S., Jovanović, J., Jacxsens, L.,& Rajković, A.. (2021). Risk assessment of dietary exposure to aflatoxin B1 in Serbia. in Food and Chemical Toxicology
Elsevier Ltd., 151, 112116.
https://doi.org/10.1016/j.fct.2021.112116

Udovički B, Tomić N, Spirović Trifunović B, Despotović S, Jovanović J, Jacxsens L, Rajković A. Risk assessment of dietary exposure to aflatoxin B1 in Serbia. in Food and Chemical Toxicology. 2021;151:112116.
doi:10.1016/j.fct.2021.112116 .

Udovički, Božidar, Tomić, Nikola, Spirović Trifunović, Bojana, Despotović, Saša, Jovanović, Jelena, Jacxsens, Liesbeth, Rajković, Andreja, "Risk assessment of dietary exposure to aflatoxin B1 in Serbia" in Food and Chemical Toxicology, 151 (2021):112116,
https://doi.org/10.1016/j.fct.2021.112116 . .

TY  - DATA
AU  - Udovički, Božidar
AU  - Tomić, Nikola
AU  - Spirović Trifunović, Bojana
AU  - Despotović, Saša
AU  - Jovanović, Jelena
AU  - Jacxsens, Liesbeth
AU  - Rajković, Andreja
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5998
PB  - Elsevier Ltd
T2  - Food and Chemical Toxicology
T1  - Supplementary data for the article: Udovički, B.; Tomić, N.; Spirović Trifunović, B.; Despotović, S.; Jovanović, J.; Jacxsens, L.; Rajković, A. Risk Assessment of Dietary Exposure to Aflatoxin B1 in Serbia. Food and Chemical Toxicology 2021, 151, 112116. https://doi.org/10.1016/j.fct.2021.112116.
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5998
ER  - 

@misc{
author = "Udovički, Božidar and Tomić, Nikola and Spirović Trifunović, Bojana and Despotović, Saša and Jovanović, Jelena and Jacxsens, Liesbeth and Rajković, Andreja",
year = "2021",
publisher = "Elsevier Ltd",
journal = "Food and Chemical Toxicology",
title = "Supplementary data for the article: Udovički, B.; Tomić, N.; Spirović Trifunović, B.; Despotović, S.; Jovanović, J.; Jacxsens, L.; Rajković, A. Risk Assessment of Dietary Exposure to Aflatoxin B1 in Serbia. Food and Chemical Toxicology 2021, 151, 112116. https://doi.org/10.1016/j.fct.2021.112116.",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5998"
}

Udovički, B., Tomić, N., Spirović Trifunović, B., Despotović, S., Jovanović, J., Jacxsens, L.,& Rajković, A.. (2021). Supplementary data for the article: Udovički, B.; Tomić, N.; Spirović Trifunović, B.; Despotović, S.; Jovanović, J.; Jacxsens, L.; Rajković, A. Risk Assessment of Dietary Exposure to Aflatoxin B1 in Serbia. Food and Chemical Toxicology 2021, 151, 112116. https://doi.org/10.1016/j.fct.2021.112116.. in Food and Chemical Toxicology
Elsevier Ltd..
https://hdl.handle.net/21.15107/rcub_agrospace_5998

Udovički B, Tomić N, Spirović Trifunović B, Despotović S, Jovanović J, Jacxsens L, Rajković A. Supplementary data for the article: Udovički, B.; Tomić, N.; Spirović Trifunović, B.; Despotović, S.; Jovanović, J.; Jacxsens, L.; Rajković, A. Risk Assessment of Dietary Exposure to Aflatoxin B1 in Serbia. Food and Chemical Toxicology 2021, 151, 112116. https://doi.org/10.1016/j.fct.2021.112116.. in Food and Chemical Toxicology. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_5998 .

Udovički, Božidar, Tomić, Nikola, Spirović Trifunović, Bojana, Despotović, Saša, Jovanović, Jelena, Jacxsens, Liesbeth, Rajković, Andreja, "Supplementary data for the article: Udovički, B.; Tomić, N.; Spirović Trifunović, B.; Despotović, S.; Jovanović, J.; Jacxsens, L.; Rajković, A. Risk Assessment of Dietary Exposure to Aflatoxin B1 in Serbia. Food and Chemical Toxicology 2021, 151, 112116. https://doi.org/10.1016/j.fct.2021.112116." in Food and Chemical Toxicology (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_5998 .