@conference{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Lazić, Vesna and Spirović Trifunović, Bojana and Miletić, Srđan and Spasić, Snežana and Jakovljević, Dragica",
year = "2022",
abstract = "Excessive production of reactive oxygen species (ROS) often results in irreversible cell
damage and leads to a number of disorders such as hypertension, heart disease, diabetes,
inflammation, premature aging and cancer. Considering the importance of diet in prevention
of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant
activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural
source of functional food ingredients. Nowadays functional food products based on the
lignicolous mushroom species are increasingly available on the market. Their powders and
extracts are consumed as dietary supplements in the form of capsules or tablets and as
additives in the formulation of healthier food products.
Antioxidant capacity of F. pinicola methanol extract was evaluated in vitro by the inhibition
of lipid peroxidation (LPx) in the linoleic acid model system. Results were normalized and
expressed as EC50 values (μg/mL). The Folin-Ciocalteu reaction adapted for a 96-well
microplate reader was used to determine the total phenol content (TPC) and result was
expressed as mg of gallic acid equivalent (GAE) per g of dry weight (DW) of extract.
Phenolic profile of the extract was analyzed by the liquid chromatography coupled with
tandem mass spectrometry (LC-MS/MS). Based on the analysis, TPC of the methanol extract
was reported to be 133.11 mg GAE/g of DW. Among hydroxybenzoic acids, the most
abundant was gallic acid, represented by 951.12 μg/g of DW. Also, presence of ellagic,
protocatechuic, p-hydroxybenzoic and syringic acid was observed. Among hydroxycinnamic
acids, chlorogenic acid was the most prevalent, with 0.76 μg/g of DW. With regard to
antioxidant activity and inhibition of LPx, EC50 value for F. pinicola methanol extract was
20.0 μg/mL.
The results of the present study suggest that methanol extract of the wild lignicolus
mushroom F. pinicola acts as natural antioxidant in the prevention of LPx. This mushroom
may be a good source for the development of antioxidant additives.",
publisher = "МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA",
journal = "The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses",
title = "Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6550"
}
