TY  - JOUR
AU  - Meland, Mekjell
AU  - Froynes, Oddmund
AU  - Fotirić-Akšić, Milica
AU  - Pojskić, Naris
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Gasi, Fuad
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5335
AB  - European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers
IS  - 2
VL  - 10
DO  - 10.3390/agronomy10020264
ER  - 

@article{
author = "Meland, Mekjell and Froynes, Oddmund and Fotirić-Akšić, Milica and Pojskić, Naris and Kalamujić-Stroil, Belma and Lasić, Lejla and Gasi, Fuad",
year = "2020",
abstract = "European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers",
number = "2",
volume = "10",
doi = "10.3390/agronomy10020264"
}

Meland, M., Froynes, O., Fotirić-Akšić, M., Pojskić, N., Kalamujić-Stroil, B., Lasić, L.,& Gasi, F.. (2020). Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel
MDPI, BASEL., 10(2).
https://doi.org/10.3390/agronomy10020264

Meland M, Froynes O, Fotirić-Akšić M, Pojskić N, Kalamujić-Stroil B, Lasić L, Gasi F. Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel. 2020;10(2).
doi:10.3390/agronomy10020264 .

Meland, Mekjell, Froynes, Oddmund, Fotirić-Akšić, Milica, Pojskić, Naris, Kalamujić-Stroil, Belma, Lasić, Lejla, Gasi, Fuad, "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers" in Agronomy-Basel, 10, no. 2 (2020),
https://doi.org/10.3390/agronomy10020264 . .

TY  - JOUR
AU  - Gasi, Fuad
AU  - Froynes, Oddmund
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Pojskić, Naris
AU  - Fotirić-Akšić, Milica
AU  - Meland, Mekjell
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5291
AB  - The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'
IS  - 9
VL  - 10
DO  - 10.3390/agronomy10091372
ER  - 

@article{
author = "Gasi, Fuad and Froynes, Oddmund and Kalamujić-Stroil, Belma and Lasić, Lejla and Pojskić, Naris and Fotirić-Akšić, Milica and Meland, Mekjell",
year = "2020",
abstract = "The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'",
number = "9",
volume = "10",
doi = "10.3390/agronomy10091372"
}

Gasi, F., Froynes, O., Kalamujić-Stroil, B., Lasić, L., Pojskić, N., Fotirić-Akšić, M.,& Meland, M.. (2020). S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel
MDPI, BASEL., 10(9).
https://doi.org/10.3390/agronomy10091372

Gasi F, Froynes O, Kalamujić-Stroil B, Lasić L, Pojskić N, Fotirić-Akšić M, Meland M. S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel. 2020;10(9).
doi:10.3390/agronomy10091372 .

Gasi, Fuad, Froynes, Oddmund, Kalamujić-Stroil, Belma, Lasić, Lejla, Pojskić, Naris, Fotirić-Akšić, Milica, Meland, Mekjell, "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'" in Agronomy-Basel, 10, no. 9 (2020),
https://doi.org/10.3390/agronomy10091372 . .