Hamidović, Saud

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  • Hamidović, Saud (13)

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POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY

Hamidović, Saud; Ibraković, Vanesa; Duvnjak, Amerisa; Opačin, Edna; Karličić, Vera; Lalević, Blažo

(The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022, 2023)

TY  - CONF
AU  - Hamidović, Saud
AU  - Ibraković, Vanesa
AU  - Duvnjak, Amerisa
AU  - Opačin, Edna
AU  - Karličić, Vera
AU  - Lalević, Blažo
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6533
AB  - One of the major public health concerns is food contamination by pathogenic bacteria. Fresh food
products can be contaminated during each phase of food production. Many factors are involved in the
survival and growth of pathogenic bacteria, such as environmental and agricultural practices. Several
methods are proposed for pathogen removal from fresh food products; one of them is the application
of essential oils. Thus, the aim of this research was to determine the effects of caraway, coriander, and
thyme essential oils on E. coli and Salmonella spp. growth. Mueller-Hinton agar, previously
inoculated by overnight bacterial cultures, was used for the estimation of particular effects. Sterile
filter paper discs impregnated with essential oil were placed on the surface of the agar. After
incubation, the inhibition zone was measured. The olive oil macerate was used as a control. The results
showed that the highest impact was noticed in treatment with caraway essential oil, while thyme
essential oil showed the lowest inhibition zone. The obtained results confirm the potential of natural
products in the suppression of pathogen growth.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022
C3  - Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
T1  - POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6533
ER  - 
@conference{
author = "Hamidović, Saud and Ibraković, Vanesa and Duvnjak, Amerisa and Opačin, Edna and Karličić, Vera and Lalević, Blažo",
year = "2023",
abstract = "One of the major public health concerns is food contamination by pathogenic bacteria. Fresh food
products can be contaminated during each phase of food production. Many factors are involved in the
survival and growth of pathogenic bacteria, such as environmental and agricultural practices. Several
methods are proposed for pathogen removal from fresh food products; one of them is the application
of essential oils. Thus, the aim of this research was to determine the effects of caraway, coriander, and
thyme essential oils on E. coli and Salmonella spp. growth. Mueller-Hinton agar, previously
inoculated by overnight bacterial cultures, was used for the estimation of particular effects. Sterile
filter paper discs impregnated with essential oil were placed on the surface of the agar. After
incubation, the inhibition zone was measured. The olive oil macerate was used as a control. The results
showed that the highest impact was noticed in treatment with caraway essential oil, while thyme
essential oil showed the lowest inhibition zone. The obtained results confirm the potential of natural
products in the suppression of pathogen growth.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022",
journal = "Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection",
title = "POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6533"
}
Hamidović, S., Ibraković, V., Duvnjak, A., Opačin, E., Karličić, V.,& Lalević, B.. (2023). POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY. in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022..
https://hdl.handle.net/21.15107/rcub_agrospace_6533
Hamidović S, Ibraković V, Duvnjak A, Opačin E, Karličić V, Lalević B. POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY. in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6533 .
Hamidović, Saud, Ibraković, Vanesa, Duvnjak, Amerisa, Opačin, Edna, Karličić, Vera, Lalević, Blažo, "POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY" in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6533 .

Response of microbial community composition in soils affected by coal mine exploitation

Hamidović, Saud; Gojgić-Cvijović, Gordana; Waisi, Hadi K.; Životić, Ljubomir; Janković-Šoja, Svjetlana; Raičević, Vera; Lalević, Blažo

(Springer, Dordrecht, 2020)

TY  - JOUR
AU  - Hamidović, Saud
AU  - Gojgić-Cvijović, Gordana
AU  - Waisi, Hadi K.
AU  - Životić, Ljubomir
AU  - Janković-Šoja, Svjetlana
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5380
AB  - Surface mining activities, despite their benefits, lead to the deterioration of local and regional environmental quality and play a role in global ecosystem pollution. This research aimed to estimate the culturable microbial population structure at five locations near the opencast coal mine "Kakanj" (Bosnia and Herzegovina) via agar plate and phospholipid fatty acids (PLFA) method and to establish its relationship to the physical and chemical properties of soil. Using the ICP-OES method, the heavy metal pollution of all examined locations (overburden, former grass yard, forest, arable soil, and greenhouse) was observed. Substantial variations among the sites regarding the most expressed indicators of heavy metal pollution were noted; Cr, Pb, Ni, and Cu content ranged from 63.17 to 524.47, 20.57 to 349.47, 139.13 to 2785.67, and 25.97 to 458.73 mg/kg, respectively. In the overburden sample, considerable low microbial activity was detected; the bacterial count was approximately 6- to 18-fold lower in comparison with the other samples. PLFA analysis showed the reduction of microbial diversity, reflected through the prevalence of normal and branched saturated fatty acids, their ratio (ranged from 0.92 to 7.13), and the absence of fungal marker 18:2 omega 6 fatty acid. The principal component analysis showed a strong negative impact of heavy metals Na and B on main microbial and PLFA profiles. In contrast, stock of main chemical parameters, including Ca, K, Fe, and pH, was positively correlated with the microbial community structure.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Response of microbial community composition in soils affected by coal mine exploitation
IS  - 6
VL  - 192
DO  - 10.1007/s10661-020-08305-2
ER  - 
@article{
author = "Hamidović, Saud and Gojgić-Cvijović, Gordana and Waisi, Hadi K. and Životić, Ljubomir and Janković-Šoja, Svjetlana and Raičević, Vera and Lalević, Blažo",
year = "2020",
abstract = "Surface mining activities, despite their benefits, lead to the deterioration of local and regional environmental quality and play a role in global ecosystem pollution. This research aimed to estimate the culturable microbial population structure at five locations near the opencast coal mine "Kakanj" (Bosnia and Herzegovina) via agar plate and phospholipid fatty acids (PLFA) method and to establish its relationship to the physical and chemical properties of soil. Using the ICP-OES method, the heavy metal pollution of all examined locations (overburden, former grass yard, forest, arable soil, and greenhouse) was observed. Substantial variations among the sites regarding the most expressed indicators of heavy metal pollution were noted; Cr, Pb, Ni, and Cu content ranged from 63.17 to 524.47, 20.57 to 349.47, 139.13 to 2785.67, and 25.97 to 458.73 mg/kg, respectively. In the overburden sample, considerable low microbial activity was detected; the bacterial count was approximately 6- to 18-fold lower in comparison with the other samples. PLFA analysis showed the reduction of microbial diversity, reflected through the prevalence of normal and branched saturated fatty acids, their ratio (ranged from 0.92 to 7.13), and the absence of fungal marker 18:2 omega 6 fatty acid. The principal component analysis showed a strong negative impact of heavy metals Na and B on main microbial and PLFA profiles. In contrast, stock of main chemical parameters, including Ca, K, Fe, and pH, was positively correlated with the microbial community structure.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Response of microbial community composition in soils affected by coal mine exploitation",
number = "6",
volume = "192",
doi = "10.1007/s10661-020-08305-2"
}
Hamidović, S., Gojgić-Cvijović, G., Waisi, H. K., Životić, L., Janković-Šoja, S., Raičević, V.,& Lalević, B.. (2020). Response of microbial community composition in soils affected by coal mine exploitation. in Environmental Monitoring and Assessment
Springer, Dordrecht., 192(6).
https://doi.org/10.1007/s10661-020-08305-2
Hamidović S, Gojgić-Cvijović G, Waisi HK, Životić L, Janković-Šoja S, Raičević V, Lalević B. Response of microbial community composition in soils affected by coal mine exploitation. in Environmental Monitoring and Assessment. 2020;192(6).
doi:10.1007/s10661-020-08305-2 .
Hamidović, Saud, Gojgić-Cvijović, Gordana, Waisi, Hadi K., Životić, Ljubomir, Janković-Šoja, Svjetlana, Raičević, Vera, Lalević, Blažo, "Response of microbial community composition in soils affected by coal mine exploitation" in Environmental Monitoring and Assessment, 192, no. 6 (2020),
https://doi.org/10.1007/s10661-020-08305-2 . .
9
3
10

Dynamics of microbial populations activities after the application of nicosulfuron

Hamidović, Saud; Lalević, Blažo; Borovac, B.; Kazlagić, A.; Haseljić, S.; Raičević, Vera; Djikić, M.

(Springer, 2020)

TY  - CONF
AU  - Hamidović, Saud
AU  - Lalević, Blažo
AU  - Borovac, B.
AU  - Kazlagić, A.
AU  - Haseljić, S.
AU  - Raičević, Vera
AU  - Djikić, M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5436
AB  - When a pesticide gets into soil, it takes part in a number of physical, chemical and biological processes that depend not only on the compound itself, but a number of other factors such as physical, chemical, and biological characteristics of soil and climatic factors. Microorganisms play an important role in pesticide degradation as they are able to utilize the biogenic elements from those compounds, as well as energy for their physiological processes. On the other hand, pesticides are more or less toxic substances that can have adverse effect on microbial populations and prevent their development, reduce their abundance, deplete their taxonomic complexity and create communities with a lower level of diversity and reduced physiological activity. The aim of this study was to analyze post nicosulfuron application dynamics of microbial populations’ activity in the soil. By standard methodology, soil pH value was determined as slightly acid to slightly alkaline, humus and available K content as moderate to high, and available P content as very low to moderate. Microbial abundance (total number of bacteria, number of ammonification bacteria, fungi and actinomycetes) was determined by agar plate method. The application of nicosulfuron in all samples and at both locations has resulted in increased number of bacteria, fungi and ammonification bacteria and reduction of the number of actinomycetes. The highest positive effect on the number of microorganisms was 15 days after treatment with nicosulfuron.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Dynamics of microbial populations activities after the application of nicosulfuron
EP  - 280
SP  - 275
VL  - 78
DO  - 10.1007/978-3-030-40049-1_35
ER  - 
@conference{
author = "Hamidović, Saud and Lalević, Blažo and Borovac, B. and Kazlagić, A. and Haseljić, S. and Raičević, Vera and Djikić, M.",
year = "2020",
abstract = "When a pesticide gets into soil, it takes part in a number of physical, chemical and biological processes that depend not only on the compound itself, but a number of other factors such as physical, chemical, and biological characteristics of soil and climatic factors. Microorganisms play an important role in pesticide degradation as they are able to utilize the biogenic elements from those compounds, as well as energy for their physiological processes. On the other hand, pesticides are more or less toxic substances that can have adverse effect on microbial populations and prevent their development, reduce their abundance, deplete their taxonomic complexity and create communities with a lower level of diversity and reduced physiological activity. The aim of this study was to analyze post nicosulfuron application dynamics of microbial populations’ activity in the soil. By standard methodology, soil pH value was determined as slightly acid to slightly alkaline, humus and available K content as moderate to high, and available P content as very low to moderate. Microbial abundance (total number of bacteria, number of ammonification bacteria, fungi and actinomycetes) was determined by agar plate method. The application of nicosulfuron in all samples and at both locations has resulted in increased number of bacteria, fungi and ammonification bacteria and reduction of the number of actinomycetes. The highest positive effect on the number of microorganisms was 15 days after treatment with nicosulfuron.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Dynamics of microbial populations activities after the application of nicosulfuron",
pages = "280-275",
volume = "78",
doi = "10.1007/978-3-030-40049-1_35"
}
Hamidović, S., Lalević, B., Borovac, B., Kazlagić, A., Haseljić, S., Raičević, V.,& Djikić, M.. (2020). Dynamics of microbial populations activities after the application of nicosulfuron. in IFMBE Proceedings
Springer., 78, 275-280.
https://doi.org/10.1007/978-3-030-40049-1_35
Hamidović S, Lalević B, Borovac B, Kazlagić A, Haseljić S, Raičević V, Djikić M. Dynamics of microbial populations activities after the application of nicosulfuron. in IFMBE Proceedings. 2020;78:275-280.
doi:10.1007/978-3-030-40049-1_35 .
Hamidović, Saud, Lalević, Blažo, Borovac, B., Kazlagić, A., Haseljić, S., Raičević, Vera, Djikić, M., "Dynamics of microbial populations activities after the application of nicosulfuron" in IFMBE Proceedings, 78 (2020):275-280,
https://doi.org/10.1007/978-3-030-40049-1_35 . .

Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons

Gavrić, Teofil; Gadžo, D.; Jurković, J.; Djikić, M.; Hadžić, D.; Lalević, Blažo; Hamidović, Saud

(Springer, 2020)

TY  - CONF
AU  - Gavrić, Teofil
AU  - Gadžo, D.
AU  - Jurković, J.
AU  - Djikić, M.
AU  - Hadžić, D.
AU  - Lalević, Blažo
AU  - Hamidović, Saud
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5432
AB  - Tartary buckwheat (Fagopyrum tataricum Gaertn) is an annual plant that is classified as a pseudocereal with high nutritional values. It is mainly cultivated in Asia, where grain and other parts of plants are used in traditional diet. In recent years, tartary buckwheat is grown on smaller areas and mostly in a mixture with common buckwheat in Bosnia and Herzegovina. The aim of this research was to determine the chemical composition and total phenols content of tartary buckwheat grown in different vegetation seasons. Field experiments were conducted in Donje Selo, near Ilijaš during 2011, 2012 and 2013 vegetation seasons. The results show that weather in some vegetation seasons have statistically significant effect on contents of protein and starch in kernel. The largest contents of protein were found in vegetation season with a lack of precipitation (2012 year), while the largest content of starch was recorded in years with relatively higher precipitation levels (2011 and 2013 years). Results show that total phenols content depended on the plant organ and vegetation season. The highest content was found in the flower (63.63 mg GAE g−1), while the smallest content was found in the kernel (5.03 mg GAE g−1).
PB  - Springer
C3  - IFMBE Proceedings
T1  - Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons
EP  - 68
SP  - 59
VL  - 78
DO  - 10.1007/978-3-030-40049-1_7
ER  - 
@conference{
author = "Gavrić, Teofil and Gadžo, D. and Jurković, J. and Djikić, M. and Hadžić, D. and Lalević, Blažo and Hamidović, Saud",
year = "2020",
abstract = "Tartary buckwheat (Fagopyrum tataricum Gaertn) is an annual plant that is classified as a pseudocereal with high nutritional values. It is mainly cultivated in Asia, where grain and other parts of plants are used in traditional diet. In recent years, tartary buckwheat is grown on smaller areas and mostly in a mixture with common buckwheat in Bosnia and Herzegovina. The aim of this research was to determine the chemical composition and total phenols content of tartary buckwheat grown in different vegetation seasons. Field experiments were conducted in Donje Selo, near Ilijaš during 2011, 2012 and 2013 vegetation seasons. The results show that weather in some vegetation seasons have statistically significant effect on contents of protein and starch in kernel. The largest contents of protein were found in vegetation season with a lack of precipitation (2012 year), while the largest content of starch was recorded in years with relatively higher precipitation levels (2011 and 2013 years). Results show that total phenols content depended on the plant organ and vegetation season. The highest content was found in the flower (63.63 mg GAE g−1), while the smallest content was found in the kernel (5.03 mg GAE g−1).",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons",
pages = "68-59",
volume = "78",
doi = "10.1007/978-3-030-40049-1_7"
}
Gavrić, T., Gadžo, D., Jurković, J., Djikić, M., Hadžić, D., Lalević, B.,& Hamidović, S.. (2020). Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons. in IFMBE Proceedings
Springer., 78, 59-68.
https://doi.org/10.1007/978-3-030-40049-1_7
Gavrić T, Gadžo D, Jurković J, Djikić M, Hadžić D, Lalević B, Hamidović S. Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons. in IFMBE Proceedings. 2020;78:59-68.
doi:10.1007/978-3-030-40049-1_7 .
Gavrić, Teofil, Gadžo, D., Jurković, J., Djikić, M., Hadžić, D., Lalević, Blažo, Hamidović, Saud, "Chemical composition and total phenols content of tartary buckwheat (fagopyrum tataricum gaertn) grown in different vegetation seasons" in IFMBE Proceedings, 78 (2020):59-68,
https://doi.org/10.1007/978-3-030-40049-1_7 . .
3
2

Survival of soil microbial population after glyphosate application

Lalević, Blažo; Hamidović, Saud; Gavrić, Teofil; Sunulahpašić, A.; Borovac, B.; Halilović, M.; Jusić, I.; Kazlagić, A.; Delić, M.

(Springer, 2020)

TY  - CONF
AU  - Lalević, Blažo
AU  - Hamidović, Saud
AU  - Gavrić, Teofil
AU  - Sunulahpašić, A.
AU  - Borovac, B.
AU  - Halilović, M.
AU  - Jusić, I.
AU  - Kazlagić, A.
AU  - Delić, M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5433
AB  - Modern agricultural production is often associated with application of agrochemicals. One of them is glyphosate, which has become one of the most widely used herbicides in weed control. However, the widespread use of glyphosate may influence the living organism’s activity. The aim of this paper was to estimate the soil microbial activity after glyphosate application. Glyphosate (Glifomark SG) was applied at the end of 2017 on two locations: Principovo polje, and Novi Travnik (Travnik municipality, Bosnia and Herzegovina), so that corn and raspberry residues can be removed. Soil sampling (0–10 and 10–30 cm) was conducted at the time of glyphosate application, also 15 and 45 days after application. On control soil glyphosate was not applied. Chemical soil analysis (pH, content of humus, available P and K) was performed using standard methodology, whilst microbial abundance (total number of bacteria, ammonification bacteria, fungi, actinomycetes and glyphosate-tolerant bacteria) was analyzed using agar plate method. The results of chemical soil analysis showed slightly acid to slightly alkaline pH value, moderate to high humus content, and low to high content of available P and K. In most of samples, bacterial number was reduced 15 days after glyphosate application, whilst at the end of experiment increase of bacterial abundance was recorded. Similar results were obtained for fungi. Rapid decrease of actinomycetes number was observed after application of glyphosate. Two bacterial strains (PP-23 and NT-11) were able to grow on mineral medium supplemented with glyphosate in concentrations of 1 and 2% (v/v), thus representing a promising candidates for bioremediation of soil contaminated with glyphosate.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Survival of soil microbial population after glyphosate application
EP  - 43
SP  - 36
VL  - 78
DO  - 10.1007/978-3-030-40049-1_4
ER  - 
@conference{
author = "Lalević, Blažo and Hamidović, Saud and Gavrić, Teofil and Sunulahpašić, A. and Borovac, B. and Halilović, M. and Jusić, I. and Kazlagić, A. and Delić, M.",
year = "2020",
abstract = "Modern agricultural production is often associated with application of agrochemicals. One of them is glyphosate, which has become one of the most widely used herbicides in weed control. However, the widespread use of glyphosate may influence the living organism’s activity. The aim of this paper was to estimate the soil microbial activity after glyphosate application. Glyphosate (Glifomark SG) was applied at the end of 2017 on two locations: Principovo polje, and Novi Travnik (Travnik municipality, Bosnia and Herzegovina), so that corn and raspberry residues can be removed. Soil sampling (0–10 and 10–30 cm) was conducted at the time of glyphosate application, also 15 and 45 days after application. On control soil glyphosate was not applied. Chemical soil analysis (pH, content of humus, available P and K) was performed using standard methodology, whilst microbial abundance (total number of bacteria, ammonification bacteria, fungi, actinomycetes and glyphosate-tolerant bacteria) was analyzed using agar plate method. The results of chemical soil analysis showed slightly acid to slightly alkaline pH value, moderate to high humus content, and low to high content of available P and K. In most of samples, bacterial number was reduced 15 days after glyphosate application, whilst at the end of experiment increase of bacterial abundance was recorded. Similar results were obtained for fungi. Rapid decrease of actinomycetes number was observed after application of glyphosate. Two bacterial strains (PP-23 and NT-11) were able to grow on mineral medium supplemented with glyphosate in concentrations of 1 and 2% (v/v), thus representing a promising candidates for bioremediation of soil contaminated with glyphosate.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Survival of soil microbial population after glyphosate application",
pages = "43-36",
volume = "78",
doi = "10.1007/978-3-030-40049-1_4"
}
Lalević, B., Hamidović, S., Gavrić, T., Sunulahpašić, A., Borovac, B., Halilović, M., Jusić, I., Kazlagić, A.,& Delić, M.. (2020). Survival of soil microbial population after glyphosate application. in IFMBE Proceedings
Springer., 78, 36-43.
https://doi.org/10.1007/978-3-030-40049-1_4
Lalević B, Hamidović S, Gavrić T, Sunulahpašić A, Borovac B, Halilović M, Jusić I, Kazlagić A, Delić M. Survival of soil microbial population after glyphosate application. in IFMBE Proceedings. 2020;78:36-43.
doi:10.1007/978-3-030-40049-1_4 .
Lalević, Blažo, Hamidović, Saud, Gavrić, Teofil, Sunulahpašić, A., Borovac, B., Halilović, M., Jusić, I., Kazlagić, A., Delić, M., "Survival of soil microbial population after glyphosate application" in IFMBE Proceedings, 78 (2020):36-43,
https://doi.org/10.1007/978-3-030-40049-1_4 . .
1

Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil

Ilić, Dora S.; Dimkić, Ivica; Waisi, Hadi K.; Gkorezis, Panagiotis M.; Hamidović, Saud; Raičević, Vera; Lalević, Blažo

(Savez hemijskih inženjera, Beograd, 2019)

TY  - JOUR
AU  - Ilić, Dora S.
AU  - Dimkić, Ivica
AU  - Waisi, Hadi K.
AU  - Gkorezis, Panagiotis M.
AU  - Hamidović, Saud
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4922
AB  - Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil
EP  - 258
IS  - 3
SP  - 247
VL  - 25
DO  - 10.2298/CICEQ180607003I
ER  - 
@article{
author = "Ilić, Dora S. and Dimkić, Ivica and Waisi, Hadi K. and Gkorezis, Panagiotis M. and Hamidović, Saud and Raičević, Vera and Lalević, Blažo",
year = "2019",
abstract = "Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil",
pages = "258-247",
number = "3",
volume = "25",
doi = "10.2298/CICEQ180607003I"
}
Ilić, D. S., Dimkić, I., Waisi, H. K., Gkorezis, P. M., Hamidović, S., Raičević, V.,& Lalević, B.. (2019). Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 25(3), 247-258.
https://doi.org/10.2298/CICEQ180607003I
Ilić DS, Dimkić I, Waisi HK, Gkorezis PM, Hamidović S, Raičević V, Lalević B. Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2019;25(3):247-258.
doi:10.2298/CICEQ180607003I .
Ilić, Dora S., Dimkić, Ivica, Waisi, Hadi K., Gkorezis, Panagiotis M., Hamidović, Saud, Raičević, Vera, Lalević, Blažo, "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 25, no. 3 (2019):247-258,
https://doi.org/10.2298/CICEQ180607003I . .
3
1
4

Antimicrobial activity of three essential oils against several human pathogens

Dzaferović, A.; Gavrić, Teofil; Nazari, S.H.; Stojanova, M.; Bezdrob, M.; Lalević, Blažo; Hamidović, Saud

(Dorma Journals, 2019)

TY  - JOUR
AU  - Dzaferović, A.
AU  - Gavrić, Teofil
AU  - Nazari, S.H.
AU  - Stojanova, M.
AU  - Bezdrob, M.
AU  - Lalević, Blažo
AU  - Hamidović, Saud
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5168
AB  - Essential oils are products derived from various parts of plants. These products have therapeutic, pharmacological and antimicrobial properties against human pathogens. In this paper, the impact of clove, spruce and pine essential oils against Escherichia coli, Salmonella spp. and Staphylococcus aureus was determined using disc diffusion method. Liquid bacterial inoculum was streaked onto Muller-Hinton agar using the sterile swab. Antimicrobial activity was estimated by measurement of inhibition zone around the discs previously impregnated by essential oil and placed onto agar. Results showed that the antimicrobial activity of essential oils depended on the type of oil and bacterial species. The significantly highest diameter of inhibition zone against tested bacteria was recorded using clove essential oil, whilst the lowest diameter was noticed using pine essential oil. Clove oil was most effective against Staphylococcus aureus, whilst spruce and pine oil against Salmonella spp. compared to other bacteria. Strong positive correlation between the effect of the spruce oil against Escherichia coli and Staphylococcus aureus, spruce and with clove oil against Staphylococcus aureus was recorded. The results of this research indicate the possible application of essential oil of clove against human pathogens.
PB  - Dorma Journals
T2  - Journal of Environmental Treatment Techniques
T1  - Antimicrobial activity of three essential oils against several human pathogens
EP  - 505
IS  - 3
SP  - 501
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5168
ER  - 
@article{
author = "Dzaferović, A. and Gavrić, Teofil and Nazari, S.H. and Stojanova, M. and Bezdrob, M. and Lalević, Blažo and Hamidović, Saud",
year = "2019",
abstract = "Essential oils are products derived from various parts of plants. These products have therapeutic, pharmacological and antimicrobial properties against human pathogens. In this paper, the impact of clove, spruce and pine essential oils against Escherichia coli, Salmonella spp. and Staphylococcus aureus was determined using disc diffusion method. Liquid bacterial inoculum was streaked onto Muller-Hinton agar using the sterile swab. Antimicrobial activity was estimated by measurement of inhibition zone around the discs previously impregnated by essential oil and placed onto agar. Results showed that the antimicrobial activity of essential oils depended on the type of oil and bacterial species. The significantly highest diameter of inhibition zone against tested bacteria was recorded using clove essential oil, whilst the lowest diameter was noticed using pine essential oil. Clove oil was most effective against Staphylococcus aureus, whilst spruce and pine oil against Salmonella spp. compared to other bacteria. Strong positive correlation between the effect of the spruce oil against Escherichia coli and Staphylococcus aureus, spruce and with clove oil against Staphylococcus aureus was recorded. The results of this research indicate the possible application of essential oil of clove against human pathogens.",
publisher = "Dorma Journals",
journal = "Journal of Environmental Treatment Techniques",
title = "Antimicrobial activity of three essential oils against several human pathogens",
pages = "505-501",
number = "3",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5168"
}
Dzaferović, A., Gavrić, T., Nazari, S.H., Stojanova, M., Bezdrob, M., Lalević, B.,& Hamidović, S.. (2019). Antimicrobial activity of three essential oils against several human pathogens. in Journal of Environmental Treatment Techniques
Dorma Journals., 7(3), 501-505.
https://hdl.handle.net/21.15107/rcub_agrospace_5168
Dzaferović A, Gavrić T, Nazari S, Stojanova M, Bezdrob M, Lalević B, Hamidović S. Antimicrobial activity of three essential oils against several human pathogens. in Journal of Environmental Treatment Techniques. 2019;7(3):501-505.
https://hdl.handle.net/21.15107/rcub_agrospace_5168 .
Dzaferović, A., Gavrić, Teofil, Nazari, S.H., Stojanova, M., Bezdrob, M., Lalević, Blažo, Hamidović, Saud, "Antimicrobial activity of three essential oils against several human pathogens" in Journal of Environmental Treatment Techniques, 7, no. 3 (2019):501-505,
https://hdl.handle.net/21.15107/rcub_agrospace_5168 .
4

Assessment of microbial diversity of soil exposed to nicosulfuron

Sunulahpašić, Amer; Hamidović, Saud; Mitrić, Siniša; Gavrić, Teofil; Haseljić, Sanel; Lalević, Blažo

(Inženjersko društvo za koroziju, Beograd, 2019)

TY  - JOUR
AU  - Sunulahpašić, Amer
AU  - Hamidović, Saud
AU  - Mitrić, Siniša
AU  - Gavrić, Teofil
AU  - Haseljić, Sanel
AU  - Lalević, Blažo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5223
AB  - Nicosulfuron belongs to the sulfonylurea pesticides, which are widely used for weeds control. Except of benefits in plant production, long-term application of nicosulfuron may have toxic effect for living organisms, including microorganisms. The aim of this paper was to determined impact of nicosulfuron on microbial diversity of soil. Sampling of soil (0-20 and 20-40 cm) treated with nicosulfuron at village Trenica (Novi Travnik municipality, Bosnia and Herzegovina) was performed in autumn 2017. Determination of microbial diversity (total number of bacteria, ammonification bacteria, fungi and actinomycetes) was performed using standard methodology, whilst nicosulfuron-tolerant bacteria were isolated using enrichment method. Soil without nicosulfuron application was used as a control. The results showed that bacteria were most abundant microbial population. In all experiments, reduction of microbial diversity in nicosulfuron-treated soil compared to untreated was observed. This reduction was most expressed in fungal number, which is reduced from 38 to 60% compared to control. Several nicosulfuron-tolerant isolates were isolated by enrichment method. By microscopic observation and using API test kits and APIWEB database, isolates 17cs, and 22wl and 5 wl were identified as Pseudomonas fluorescens, and Bacillus subtilis, respectively. These bacterial isolates could be applied in remediation of environments polluted by nicosulfuron.
AB  - Nikosulfuron pripada grupi pesticide sulfonil urea, koji imaju široku primenu u uništavanju korova. Iako je njegova primena korisna sa aspekta uspešne biljne proizvodnje, njegova višegodišnja upotreba može imati toksične efekte za živi svet, uključujući i mikroorganizme. Cilj ovog rada bio je ispitivanje uticaja nikosulfurona na mikrobni diverzitet zemljišta. Uzorkovanje zemljišta (0-20 i 20-40 cm) tretiranog nikosulfuronom na području sela Trenica (Opština Novi Travnik, Bosna i Hercegovina) obavljeno je u jesen 2017. godine. Određivanje mikrobnog diverziteta (ukupnog broja bakterija, amonifikatora, gljiva I aktinomiceta) izvršeno je korišćenjem standardnih metoda, dok su bakterije tolerantne na prisustvo nikosulfurona određene metodom obogaćenja. Kontrolu je predstavljalo zemljište koje nije tretirano nikosulfuronom. Rezultati ukazuju da su bakterije bile najbrojnija populacija mikroorganizama. U svim varijantama ogleda konstatovana je redukcija mikrobnog diverziteta u zemljištu koje je tretirano nikosulfuronom u odnosu na kontrolni uzorak. Stepen redukcije bio je najveći kod gljiva, čija je brojnost redukovana za 38-60% u odnosu na kontrolu. Nekoliko izolata bakterija tolerantnih na prisustvo nikosulfurona je izolovano iz uzoraka zemljišta metodom obogaćenja. Mikroskopskim ispitivanjem I pomoću API i APIWEB metode, izolati 17cs, odnosno 22wl i 5wl, su identifikovani kao Pseudomonas fluorescens, odnosno Bacillus subtilis. Ovi bakterijski izolati bi mogli da imaju potencijalnu primenu u remedijaciji ekosistema kontaminiranih nikosulfuronom.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Assessment of microbial diversity of soil exposed to nicosulfuron
T1  - Ispitivanje mikrobnog diverziteta zemljišta tretiranog nikosulfuronom
EP  - 156
IS  - 2
SP  - 152
VL  - 60
DO  - 10.5937/zasmat1902152S
ER  - 
@article{
author = "Sunulahpašić, Amer and Hamidović, Saud and Mitrić, Siniša and Gavrić, Teofil and Haseljić, Sanel and Lalević, Blažo",
year = "2019",
abstract = "Nicosulfuron belongs to the sulfonylurea pesticides, which are widely used for weeds control. Except of benefits in plant production, long-term application of nicosulfuron may have toxic effect for living organisms, including microorganisms. The aim of this paper was to determined impact of nicosulfuron on microbial diversity of soil. Sampling of soil (0-20 and 20-40 cm) treated with nicosulfuron at village Trenica (Novi Travnik municipality, Bosnia and Herzegovina) was performed in autumn 2017. Determination of microbial diversity (total number of bacteria, ammonification bacteria, fungi and actinomycetes) was performed using standard methodology, whilst nicosulfuron-tolerant bacteria were isolated using enrichment method. Soil without nicosulfuron application was used as a control. The results showed that bacteria were most abundant microbial population. In all experiments, reduction of microbial diversity in nicosulfuron-treated soil compared to untreated was observed. This reduction was most expressed in fungal number, which is reduced from 38 to 60% compared to control. Several nicosulfuron-tolerant isolates were isolated by enrichment method. By microscopic observation and using API test kits and APIWEB database, isolates 17cs, and 22wl and 5 wl were identified as Pseudomonas fluorescens, and Bacillus subtilis, respectively. These bacterial isolates could be applied in remediation of environments polluted by nicosulfuron., Nikosulfuron pripada grupi pesticide sulfonil urea, koji imaju široku primenu u uništavanju korova. Iako je njegova primena korisna sa aspekta uspešne biljne proizvodnje, njegova višegodišnja upotreba može imati toksične efekte za živi svet, uključujući i mikroorganizme. Cilj ovog rada bio je ispitivanje uticaja nikosulfurona na mikrobni diverzitet zemljišta. Uzorkovanje zemljišta (0-20 i 20-40 cm) tretiranog nikosulfuronom na području sela Trenica (Opština Novi Travnik, Bosna i Hercegovina) obavljeno je u jesen 2017. godine. Određivanje mikrobnog diverziteta (ukupnog broja bakterija, amonifikatora, gljiva I aktinomiceta) izvršeno je korišćenjem standardnih metoda, dok su bakterije tolerantne na prisustvo nikosulfurona određene metodom obogaćenja. Kontrolu je predstavljalo zemljište koje nije tretirano nikosulfuronom. Rezultati ukazuju da su bakterije bile najbrojnija populacija mikroorganizama. U svim varijantama ogleda konstatovana je redukcija mikrobnog diverziteta u zemljištu koje je tretirano nikosulfuronom u odnosu na kontrolni uzorak. Stepen redukcije bio je najveći kod gljiva, čija je brojnost redukovana za 38-60% u odnosu na kontrolu. Nekoliko izolata bakterija tolerantnih na prisustvo nikosulfurona je izolovano iz uzoraka zemljišta metodom obogaćenja. Mikroskopskim ispitivanjem I pomoću API i APIWEB metode, izolati 17cs, odnosno 22wl i 5wl, su identifikovani kao Pseudomonas fluorescens, odnosno Bacillus subtilis. Ovi bakterijski izolati bi mogli da imaju potencijalnu primenu u remedijaciji ekosistema kontaminiranih nikosulfuronom.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Assessment of microbial diversity of soil exposed to nicosulfuron, Ispitivanje mikrobnog diverziteta zemljišta tretiranog nikosulfuronom",
pages = "156-152",
number = "2",
volume = "60",
doi = "10.5937/zasmat1902152S"
}
Sunulahpašić, A., Hamidović, S., Mitrić, S., Gavrić, T., Haseljić, S.,& Lalević, B.. (2019). Assessment of microbial diversity of soil exposed to nicosulfuron. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 60(2), 152-156.
https://doi.org/10.5937/zasmat1902152S
Sunulahpašić A, Hamidović S, Mitrić S, Gavrić T, Haseljić S, Lalević B. Assessment of microbial diversity of soil exposed to nicosulfuron. in Zaštita materijala. 2019;60(2):152-156.
doi:10.5937/zasmat1902152S .
Sunulahpašić, Amer, Hamidović, Saud, Mitrić, Siniša, Gavrić, Teofil, Haseljić, Sanel, Lalević, Blažo, "Assessment of microbial diversity of soil exposed to nicosulfuron" in Zaštita materijala, 60, no. 2 (2019):152-156,
https://doi.org/10.5937/zasmat1902152S . .

Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting

Gavrić, Teofil; Jurković, J.; Hamidović, Saud; Haseljić, S.; Lalević, Blažo; Čorbo, A.; Bezdrob, M.

(Vasile Goldis Western University of Arad, 2018)

TY  - JOUR
AU  - Gavrić, Teofil
AU  - Jurković, J.
AU  - Hamidović, Saud
AU  - Haseljić, S.
AU  - Lalević, Blažo
AU  - Čorbo, A.
AU  - Bezdrob, M.
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4800
AB  - Basil (Occimum basilicum L.) is an annual plant from Lamiaceae family. It is used as a spice, aromatic and medicinal plant. The yield and quality of basil depends on variety of basil, growing technology and environmental factors such as: Temperature, rainfall, photoperiod, relative humidity and irradiance. The aim of this study was to determine the yield and contents of some bioactive phytochemical components in basil (Ocimum basilicum var. genovese), depending on time of cutting. Field experiments were conducted in Butmir, near Sarajevo. In this research yield of fresh and dry mass, contents of essential oil, total phenols, total flavonoids and antioxidant activity were determined. Experimental results suggested that the time of cutting has a significant impact on yield and bioactive components of basil. The yield of fresh mass ranged from 182.13 g (second cut) to 283.16 g per plant (first cut). Contents of bioactive components were also significantly depending on the time of cutting. High total phenols content, total flavonoids and antioxidant activity in basil were recorded in the second cut.
PB  - Vasile Goldis Western University of Arad
T2  - Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
T1  - Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting
EP  - 197
IS  - 4
SP  - 192
VL  - 28
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4800
ER  - 
@article{
author = "Gavrić, Teofil and Jurković, J. and Hamidović, Saud and Haseljić, S. and Lalević, Blažo and Čorbo, A. and Bezdrob, M.",
year = "2018",
abstract = "Basil (Occimum basilicum L.) is an annual plant from Lamiaceae family. It is used as a spice, aromatic and medicinal plant. The yield and quality of basil depends on variety of basil, growing technology and environmental factors such as: Temperature, rainfall, photoperiod, relative humidity and irradiance. The aim of this study was to determine the yield and contents of some bioactive phytochemical components in basil (Ocimum basilicum var. genovese), depending on time of cutting. Field experiments were conducted in Butmir, near Sarajevo. In this research yield of fresh and dry mass, contents of essential oil, total phenols, total flavonoids and antioxidant activity were determined. Experimental results suggested that the time of cutting has a significant impact on yield and bioactive components of basil. The yield of fresh mass ranged from 182.13 g (second cut) to 283.16 g per plant (first cut). Contents of bioactive components were also significantly depending on the time of cutting. High total phenols content, total flavonoids and antioxidant activity in basil were recorded in the second cut.",
publisher = "Vasile Goldis Western University of Arad",
journal = "Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii",
title = "Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting",
pages = "197-192",
number = "4",
volume = "28",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4800"
}
Gavrić, T., Jurković, J., Hamidović, S., Haseljić, S., Lalević, B., Čorbo, A.,& Bezdrob, M.. (2018). Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Vasile Goldis Western University of Arad., 28(4), 192-197.
https://hdl.handle.net/21.15107/rcub_agrospace_4800
Gavrić T, Jurković J, Hamidović S, Haseljić S, Lalević B, Čorbo A, Bezdrob M. Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii. 2018;28(4):192-197.
https://hdl.handle.net/21.15107/rcub_agrospace_4800 .
Gavrić, Teofil, Jurković, J., Hamidović, Saud, Haseljić, S., Lalević, Blažo, Čorbo, A., Bezdrob, M., "Yield and contents of some bioactive components of basil (Ocimum basilicum l.) depending on time of cutting" in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii, 28, no. 4 (2018):192-197,
https://hdl.handle.net/21.15107/rcub_agrospace_4800 .
7

Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study

Hamidović, Saud; Teodorović, Smilja; Lalević, Blažo; Jovičić-Petrović, Jelena; Jović, Jelena; Kiković, Dragan; Raičević, Vera

(Hard, Olsztyn 5, 2016)

TY  - JOUR
AU  - Hamidović, Saud
AU  - Teodorović, Smilja
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Jović, Jelena
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4103
AB  - Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.
PB  - Hard, Olsztyn 5
T2  - Polish Journal of Environmental Studies
T1  - Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study
EP  - 119
IS  - 1
SP  - 113
VL  - 25
DO  - 10.15244/pjoes/59465
ER  - 
@article{
author = "Hamidović, Saud and Teodorović, Smilja and Lalević, Blažo and Jovičić-Petrović, Jelena and Jović, Jelena and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.",
publisher = "Hard, Olsztyn 5",
journal = "Polish Journal of Environmental Studies",
title = "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study",
pages = "119-113",
number = "1",
volume = "25",
doi = "10.15244/pjoes/59465"
}
Hamidović, S., Teodorović, S., Lalević, B., Jovičić-Petrović, J., Jović, J., Kiković, D.,& Raičević, V.. (2016). Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies
Hard, Olsztyn 5., 25(1), 113-119.
https://doi.org/10.15244/pjoes/59465
Hamidović S, Teodorović S, Lalević B, Jovičić-Petrović J, Jović J, Kiković D, Raičević V. Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies. 2016;25(1):113-119.
doi:10.15244/pjoes/59465 .
Hamidović, Saud, Teodorović, Smilja, Lalević, Blažo, Jovičić-Petrović, Jelena, Jović, Jelena, Kiković, Dragan, Raičević, Vera, "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study" in Polish Journal of Environmental Studies, 25, no. 1 (2016):113-119,
https://doi.org/10.15244/pjoes/59465 . .
2
2
3

Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina)

Hamidović, Saud; Čolo, Josip; Kiković, Dragan; Krivošej, Zoran; Lalević, Blažo; Milinković, Mira

(Inženjersko društvo za koroziju, Beograd, 2013)

TY  - JOUR
AU  - Hamidović, Saud
AU  - Čolo, Josip
AU  - Kiković, Dragan
AU  - Krivošej, Zoran
AU  - Lalević, Blažo
AU  - Milinković, Mira
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3263
AB  - Ecoremediation is one of reclamation methods, using plant-microbes-based technologies, and its application is suitable for mining activities-degraded ecosystems. The aim of this work was to estimate plant and microbial diversity on coal mine field 'Kakanj ' (Bosnia and Herzegovina). The results show the domination of Asteraceae species. In most of samples, bacterial number was higher in rhizosphere comparing to the surrounding bulk soil, while the abundance of fungi was higher comparing to the number of actinomycetes. Lowest microbial activity was detected in waste material. Determination of plant and microbial diversity provide valuable information for restoration of mining activities-degraded environments.
AB  - Ekoremedijacija je jedna od metoda za obnavljanje ekosistema i efikasna je za područja pod eksploatacijom uglja. Cilj ovog rada je ispitivanje biljno-mikrobnog diverziteta na području rudnika mrkog uglja 'Kakanj' (Bosna i Hercegovina). Determinacija biljnih vrsta obavljena je pomoću ključa za identifikaciju, dok su mikrobiološke osobine zemljišta i otpadnog materijala nastalog pranjem uglja ispitane standardnim metodama. Rezultati ukazuju na dominaciju biljaka iz familije Asteraceae. U najvećem broju uzoraka brojnost bakterija bila je veća u zoni rizosfere u odnosu na okolno zemljište. Zastupljenost gljiva bila je veća u poređenju sa brojem aktinomiceta. Najslabija mikrobiološka aktivnost zabeležena je u uzorcima otpadnog materijala. Determinacija biljnog i mikrobnog diverziteta obezbeđuje informacije značajne za obnavljanje ekosistema narušenog eksploatacijom uglja.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina)
T1  - Biljni i mikrobni diverzitet zemljišta pod eksploatacijom uglja u rudniku 'Kakanj' (Bosna i Hercegovina)
EP  - 408
IS  - 4
SP  - 403
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3263
ER  - 
@article{
author = "Hamidović, Saud and Čolo, Josip and Kiković, Dragan and Krivošej, Zoran and Lalević, Blažo and Milinković, Mira",
year = "2013",
abstract = "Ecoremediation is one of reclamation methods, using plant-microbes-based technologies, and its application is suitable for mining activities-degraded ecosystems. The aim of this work was to estimate plant and microbial diversity on coal mine field 'Kakanj ' (Bosnia and Herzegovina). The results show the domination of Asteraceae species. In most of samples, bacterial number was higher in rhizosphere comparing to the surrounding bulk soil, while the abundance of fungi was higher comparing to the number of actinomycetes. Lowest microbial activity was detected in waste material. Determination of plant and microbial diversity provide valuable information for restoration of mining activities-degraded environments., Ekoremedijacija je jedna od metoda za obnavljanje ekosistema i efikasna je za područja pod eksploatacijom uglja. Cilj ovog rada je ispitivanje biljno-mikrobnog diverziteta na području rudnika mrkog uglja 'Kakanj' (Bosna i Hercegovina). Determinacija biljnih vrsta obavljena je pomoću ključa za identifikaciju, dok su mikrobiološke osobine zemljišta i otpadnog materijala nastalog pranjem uglja ispitane standardnim metodama. Rezultati ukazuju na dominaciju biljaka iz familije Asteraceae. U najvećem broju uzoraka brojnost bakterija bila je veća u zoni rizosfere u odnosu na okolno zemljište. Zastupljenost gljiva bila je veća u poređenju sa brojem aktinomiceta. Najslabija mikrobiološka aktivnost zabeležena je u uzorcima otpadnog materijala. Determinacija biljnog i mikrobnog diverziteta obezbeđuje informacije značajne za obnavljanje ekosistema narušenog eksploatacijom uglja.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina), Biljni i mikrobni diverzitet zemljišta pod eksploatacijom uglja u rudniku 'Kakanj' (Bosna i Hercegovina)",
pages = "408-403",
number = "4",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3263"
}
Hamidović, S., Čolo, J., Kiković, D., Krivošej, Z., Lalević, B.,& Milinković, M.. (2013). Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina). in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 54(4), 403-408.
https://hdl.handle.net/21.15107/rcub_agrospace_3263
Hamidović S, Čolo J, Kiković D, Krivošej Z, Lalević B, Milinković M. Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina). in Zaštita materijala. 2013;54(4):403-408.
https://hdl.handle.net/21.15107/rcub_agrospace_3263 .
Hamidović, Saud, Čolo, Josip, Kiković, Dragan, Krivošej, Zoran, Lalević, Blažo, Milinković, Mira, "Plant and microbial diversity in coal mine-affected soil in 'Kakanj' (Bosnia and Herzegovina)" in Zaštita materijala, 54, no. 4 (2013):403-408,
https://hdl.handle.net/21.15107/rcub_agrospace_3263 .

Capability of Kocuria sp.: In MTBE biodegradation

Lalević, Blažo; Raičević, Vera; Kiković, Dragan; Spasojević, Ivana; Hamidović, Saud; Atanasković, Iva

(Unija bioloških naučnih društava Jugoslavije, Beograd, 2012)

TY  - JOUR
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kiković, Dragan
AU  - Spasojević, Ivana
AU  - Hamidović, Saud
AU  - Atanasković, Iva
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2879
AB  - Methyl tert-butyl ether (MTBE) is one of most commonly used oxygenates added to replace toxic compounds from gasoline and to reduce air pollutants emission. Due to intensive use and persistence, MTBE has become one of the most important environment pollutants. Presented research shows the capability of bacterial isolate Kocuria sp. 27/1 of biodegradation of MTBE as a additional source of carbon and energy. The obtained results shows that Kocuria sp. 27/1 was capable of utilizing of different initial concentrations of MTBE added as additional carbon and energy source. After 86 days of incubation on rotary shaker at 110 rpm and 27 °C, degradation rates of initial 25; 125 and 250 μg concentrations MTBE x ml-1 were 32; 28 and 62%, respectively. Highest degradation rate at all examinated concentrations was noticed in the beginning of incubation period. During the incubation increasing of bacterial number in all treatments was noticed, but this increasing was very slow, probably because of intermedier(s) during degradation and recalcitrance of C-atom. This research confirms the potential use of Kocuria sp. 27/1 in bioremediation of MTBE-contaminated environments.
AB  - Metil tercijarni butil etar (MTBE) je jedan od najčešće korišćenih oksigenata koji je dodat benzinu u cilju zamene toksičnih komponenti i redukcije aerozagađenja. Zbog intenzivne upotrebe i otpornosti, MTBE je postao jedan od najvažnijih polutanata u čovekovoj sredini. Ova istraživanja pokazala su potencijal bakterijskog izolata Kocuria sp. 27/1 u biodegradaciji MTBE-a kao dounskog izvora ugljenika i energije. Dobijeni rezultati pokazuju da je Kocuria sp. 27/1 bila sposobna da koristi različite početne koncentracije MTBE-a dodatog kao dopunski izvor ugljenika i energije. Posle 86 dana inkubacije u orbitalnom šejkeru pri 110 rpm i 27 °C, stepen degradacije početnih koncentracija MTBE-a (25, 125 i 250 μg x ml-1) iznosio je između 28 i 62%. Najveći stepen degradacije u svim ispitivanim koncentracijama zabeležen je na početku inkubacionog perioda. Tokom inkubacije konstatovan je porast broja bakterija, ali je ovaj porast bio veoma slab, što je verovatno povezano sa nastankom intermedijera tokom degradacije i otpornošću C atoma. Ova istraživanja potvrđuju potencijalnu primenu bakterije Kocuria sp. 27/1 u bioremedijaciji ekosistema kontaminiranih MTBE-om.
PB  - Unija bioloških naučnih društava Jugoslavije, Beograd
T2  - Zemljište i biljka
T1  - Capability of Kocuria sp.: In MTBE biodegradation
T1  - Sposobnost Kocuria sp. - u biodegradaciji MTBE-a
EP  - 105
IS  - 2
SP  - 99
VL  - 61
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2879
ER  - 
@article{
author = "Lalević, Blažo and Raičević, Vera and Kiković, Dragan and Spasojević, Ivana and Hamidović, Saud and Atanasković, Iva",
year = "2012",
abstract = "Methyl tert-butyl ether (MTBE) is one of most commonly used oxygenates added to replace toxic compounds from gasoline and to reduce air pollutants emission. Due to intensive use and persistence, MTBE has become one of the most important environment pollutants. Presented research shows the capability of bacterial isolate Kocuria sp. 27/1 of biodegradation of MTBE as a additional source of carbon and energy. The obtained results shows that Kocuria sp. 27/1 was capable of utilizing of different initial concentrations of MTBE added as additional carbon and energy source. After 86 days of incubation on rotary shaker at 110 rpm and 27 °C, degradation rates of initial 25; 125 and 250 μg concentrations MTBE x ml-1 were 32; 28 and 62%, respectively. Highest degradation rate at all examinated concentrations was noticed in the beginning of incubation period. During the incubation increasing of bacterial number in all treatments was noticed, but this increasing was very slow, probably because of intermedier(s) during degradation and recalcitrance of C-atom. This research confirms the potential use of Kocuria sp. 27/1 in bioremediation of MTBE-contaminated environments., Metil tercijarni butil etar (MTBE) je jedan od najčešće korišćenih oksigenata koji je dodat benzinu u cilju zamene toksičnih komponenti i redukcije aerozagađenja. Zbog intenzivne upotrebe i otpornosti, MTBE je postao jedan od najvažnijih polutanata u čovekovoj sredini. Ova istraživanja pokazala su potencijal bakterijskog izolata Kocuria sp. 27/1 u biodegradaciji MTBE-a kao dounskog izvora ugljenika i energije. Dobijeni rezultati pokazuju da je Kocuria sp. 27/1 bila sposobna da koristi različite početne koncentracije MTBE-a dodatog kao dopunski izvor ugljenika i energije. Posle 86 dana inkubacije u orbitalnom šejkeru pri 110 rpm i 27 °C, stepen degradacije početnih koncentracija MTBE-a (25, 125 i 250 μg x ml-1) iznosio je između 28 i 62%. Najveći stepen degradacije u svim ispitivanim koncentracijama zabeležen je na početku inkubacionog perioda. Tokom inkubacije konstatovan je porast broja bakterija, ali je ovaj porast bio veoma slab, što je verovatno povezano sa nastankom intermedijera tokom degradacije i otpornošću C atoma. Ova istraživanja potvrđuju potencijalnu primenu bakterije Kocuria sp. 27/1 u bioremedijaciji ekosistema kontaminiranih MTBE-om.",
publisher = "Unija bioloških naučnih društava Jugoslavije, Beograd",
journal = "Zemljište i biljka",
title = "Capability of Kocuria sp.: In MTBE biodegradation, Sposobnost Kocuria sp. - u biodegradaciji MTBE-a",
pages = "105-99",
number = "2",
volume = "61",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2879"
}
Lalević, B., Raičević, V., Kiković, D., Spasojević, I., Hamidović, S.,& Atanasković, I.. (2012). Capability of Kocuria sp.: In MTBE biodegradation. in Zemljište i biljka
Unija bioloških naučnih društava Jugoslavije, Beograd., 61(2), 99-105.
https://hdl.handle.net/21.15107/rcub_agrospace_2879
Lalević B, Raičević V, Kiković D, Spasojević I, Hamidović S, Atanasković I. Capability of Kocuria sp.: In MTBE biodegradation. in Zemljište i biljka. 2012;61(2):99-105.
https://hdl.handle.net/21.15107/rcub_agrospace_2879 .
Lalević, Blažo, Raičević, Vera, Kiković, Dragan, Spasojević, Ivana, Hamidović, Saud, Atanasković, Iva, "Capability of Kocuria sp.: In MTBE biodegradation" in Zemljište i biljka, 61, no. 2 (2012):99-105,
https://hdl.handle.net/21.15107/rcub_agrospace_2879 .

Biodegradation of methyl-tert-butyl ether by Kocuria sp.

Lalević, Blažo; Jović, Jelena; Raičević, Vera; Kljujev, Igor; Kiković, Dragan; Hamidović, Saud

(Savez hemijskih inženjera, Beograd, 2012)

TY  - JOUR
AU  - Lalević, Blažo
AU  - Jović, Jelena
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Kiković, Dragan
AU  - Hamidović, Saud
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2922
AB  - Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE.
AB  - Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Biodegradation of methyl-tert-butyl ether by Kocuria sp.
T1  - Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.
EP  - 722
IS  - 5
SP  - 717
VL  - 66
DO  - 10.2298/HEMIND120110019L
ER  - 
@article{
author = "Lalević, Blažo and Jović, Jelena and Raičević, Vera and Kljujev, Igor and Kiković, Dragan and Hamidović, Saud",
year = "2012",
abstract = "Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE., Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Biodegradation of methyl-tert-butyl ether by Kocuria sp., Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.",
pages = "722-717",
number = "5",
volume = "66",
doi = "10.2298/HEMIND120110019L"
}
Lalević, B., Jović, J., Raičević, V., Kljujev, I., Kiković, D.,& Hamidović, S.. (2012). Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 66(5), 717-722.
https://doi.org/10.2298/HEMIND120110019L
Lalević B, Jović J, Raičević V, Kljujev I, Kiković D, Hamidović S. Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija. 2012;66(5):717-722.
doi:10.2298/HEMIND120110019L .
Lalević, Blažo, Jović, Jelena, Raičević, Vera, Kljujev, Igor, Kiković, Dragan, Hamidović, Saud, "Biodegradation of methyl-tert-butyl ether by Kocuria sp." in Hemijska industrija, 66, no. 5 (2012):717-722,
https://doi.org/10.2298/HEMIND120110019L . .
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