TY  - JOUR
AU  - Živković, Ivana
AU  - Šavikin, Katarina
AU  - Živković, Jelena
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Lazić, Dejan
AU  - Radin, Dragoslava
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5834
AB  - Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.
PB  - Springer
T2  - Plant Foods for Human Nutrition
T1  - Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids
DO  - 10.1007/s11130-021-00895-7
ER  - 

@article{
author = "Živković, Ivana and Šavikin, Katarina and Živković, Jelena and Zdunić, Gordana and Janković, Teodora and Lazić, Dejan and Radin, Dragoslava",
year = "2021",
abstract = "Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.",
publisher = "Springer",
journal = "Plant Foods for Human Nutrition",
title = "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids",
doi = "10.1007/s11130-021-00895-7"
}

Živković, I., Šavikin, K., Živković, J., Zdunić, G., Janković, T., Lazić, D.,& Radin, D.. (2021). Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition
Springer..
https://doi.org/10.1007/s11130-021-00895-7

Živković I, Šavikin K, Živković J, Zdunić G, Janković T, Lazić D, Radin D. Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition. 2021;.
doi:10.1007/s11130-021-00895-7 .

Živković, Ivana, Šavikin, Katarina, Živković, Jelena, Zdunić, Gordana, Janković, Teodora, Lazić, Dejan, Radin, Dragoslava, "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids" in Plant Foods for Human Nutrition (2021),
https://doi.org/10.1007/s11130-021-00895-7 . .

TY  - JOUR
AU  - Živković, Ivana
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Živković, Jelena
AU  - Bigović, Dubravka
AU  - Menković, Nebojša
AU  - Radin, Dragoslava
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4828
AB  - Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Antiviral activity of medicinal plants extracts against foodborne norovirus
EP  - 34
IS  - 38
SP  - 31
DO  - 10.5937/leksir1838031Z
ER  - 

@article{
author = "Živković, Ivana and Šavikin, Katarina and Zdunić, Gordana and Živković, Jelena and Bigović, Dubravka and Menković, Nebojša and Radin, Dragoslava",
year = "2018",
abstract = "Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Antiviral activity of medicinal plants extracts against foodborne norovirus",
pages = "34-31",
number = "38",
doi = "10.5937/leksir1838031Z"
}

Živković, I., Šavikin, K., Zdunić, G., Živković, J., Bigović, D., Menković, N.,& Radin, D.. (2018). Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 31-34.
https://doi.org/10.5937/leksir1838031Z

Živković I, Šavikin K, Zdunić G, Živković J, Bigović D, Menković N, Radin D. Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine. 2018;(38):31-34.
doi:10.5937/leksir1838031Z .

Živković, Ivana, Šavikin, Katarina, Zdunić, Gordana, Živković, Jelena, Bigović, Dubravka, Menković, Nebojša, Radin, Dragoslava, "Antiviral activity of medicinal plants extracts against foodborne norovirus" in Lekovite sirovine, no. 38 (2018):31-34,
https://doi.org/10.5937/leksir1838031Z . .

TY  - JOUR
AU  - Djordjević, Boban
AU  - Savikin, Katarina
AU  - Djurović, Dejan
AU  - Veberić, Robert
AU  - Mikulić-Petkovsek, Maja
AU  - Zdunić, Gordana
AU  - Vulić, Todor
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6053
AB  - BACKGROUND: Changes of environmental factors, created under the influence of various shading nets, could significantly affect the biological characteristics of plants grown in such conditions as well as biosynthesis of primary metabolites and ascorbic acid. Five blackcurrant cultivars - 'Ben Sarek', 'Ben Nevis', 'Ben Lomond', 'Ometa' and 'aanska Crna' - were cultivated in the shade of two green polyethylene nets and exposed to direct sunlight during two experimental seasons. RESULTSIn the control treatment, all cultivars contained the highest amounts of soluble solid content and number of flower buds per shoot in both years of cultivation. The bushes exposed to direct sunlight had the highest sunburn damage to the berries and leaves, and loss of yield. The greatest yield loss caused by berry damage in the present study during the experimental period was in cultivar 'Ben Sarek': 9.0% in 2010 and 15.4% in 2011. Growing in the shade of light-green net contributed the highest content of ascorbic acid. Control bushes and those shaded by light-green net had significantly higher radical scavenging activity, with values between 1.15 and 1.22 mg mL(-1). CONCLUSIONPlants shaded by nets in both years of cultivation had lower damage to leaves and berries and percentage of loss of yield, and usage of the net would be economically advantageous to growers. Fruit of blackcurrant cultivars grown in shading conditions still represent a good source of valuable nutritive and biologically active compounds.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Biological and nutritional properties of blackcurrant berries (Ribes nigrum L.) under conditions of shading nets
EP  - 2423
IS  - 12
SP  - 2416
VL  - 95
DO  - 10.1002/jsfa.6962
ER  - 

@article{
author = "Djordjević, Boban and Savikin, Katarina and Djurović, Dejan and Veberić, Robert and Mikulić-Petkovsek, Maja and Zdunić, Gordana and Vulić, Todor",
year = "2015",
abstract = "BACKGROUND: Changes of environmental factors, created under the influence of various shading nets, could significantly affect the biological characteristics of plants grown in such conditions as well as biosynthesis of primary metabolites and ascorbic acid. Five blackcurrant cultivars - 'Ben Sarek', 'Ben Nevis', 'Ben Lomond', 'Ometa' and 'aanska Crna' - were cultivated in the shade of two green polyethylene nets and exposed to direct sunlight during two experimental seasons. RESULTSIn the control treatment, all cultivars contained the highest amounts of soluble solid content and number of flower buds per shoot in both years of cultivation. The bushes exposed to direct sunlight had the highest sunburn damage to the berries and leaves, and loss of yield. The greatest yield loss caused by berry damage in the present study during the experimental period was in cultivar 'Ben Sarek': 9.0% in 2010 and 15.4% in 2011. Growing in the shade of light-green net contributed the highest content of ascorbic acid. Control bushes and those shaded by light-green net had significantly higher radical scavenging activity, with values between 1.15 and 1.22 mg mL(-1). CONCLUSIONPlants shaded by nets in both years of cultivation had lower damage to leaves and berries and percentage of loss of yield, and usage of the net would be economically advantageous to growers. Fruit of blackcurrant cultivars grown in shading conditions still represent a good source of valuable nutritive and biologically active compounds.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Biological and nutritional properties of blackcurrant berries (Ribes nigrum L.) under conditions of shading nets",
pages = "2423-2416",
number = "12",
volume = "95",
doi = "10.1002/jsfa.6962"
}

Djordjević, B., Savikin, K., Djurović, D., Veberić, R., Mikulić-Petkovsek, M., Zdunić, G.,& Vulić, T.. (2015). Biological and nutritional properties of blackcurrant berries (Ribes nigrum L.) under conditions of shading nets. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 95(12), 2416-2423.
https://doi.org/10.1002/jsfa.6962

Djordjević B, Savikin K, Djurović D, Veberić R, Mikulić-Petkovsek M, Zdunić G, Vulić T. Biological and nutritional properties of blackcurrant berries (Ribes nigrum L.) under conditions of shading nets. in Journal of the Science of Food and Agriculture. 2015;95(12):2416-2423.
doi:10.1002/jsfa.6962 .

Djordjević, Boban, Savikin, Katarina, Djurović, Dejan, Veberić, Robert, Mikulić-Petkovsek, Maja, Zdunić, Gordana, Vulić, Todor, "Biological and nutritional properties of blackcurrant berries (Ribes nigrum L.) under conditions of shading nets" in Journal of the Science of Food and Agriculture, 95, no. 12 (2015):2416-2423,
https://doi.org/10.1002/jsfa.6962 . .