TY  - JOUR
AU  - Vasić, Tanja
AU  - Vojinović, Uroš
AU  - Zujović, Suzana
AU  - Krnjaja, Vesna
AU  - Zivković, Sanja
AU  - Marković, Jordan
AU  - Stević, Milan
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4954
AB  - Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
T1  - In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum
EP  - 971
IS  - 12
SP  - 964
VL  - 54
DO  - 10.1080/03601234.2019.1653735
ER  - 

@article{
author = "Vasić, Tanja and Vojinović, Uroš and Zujović, Suzana and Krnjaja, Vesna and Zivković, Sanja and Marković, Jordan and Stević, Milan",
year = "2019",
abstract = "Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was",
title = "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum",
pages = "971-964",
number = "12",
volume = "54",
doi = "10.1080/03601234.2019.1653735"
}

Vasić, T., Vojinović, U., Zujović, S., Krnjaja, V., Zivković, S., Marković, J.,& Stević, M.. (2019). In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
Taylor & Francis Inc, Philadelphia., 54(12), 964-971.
https://doi.org/10.1080/03601234.2019.1653735

Vasić T, Vojinović U, Zujović S, Krnjaja V, Zivković S, Marković J, Stević M. In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2019;54(12):964-971.
doi:10.1080/03601234.2019.1653735 .

Vasić, Tanja, Vojinović, Uroš, Zujović, Suzana, Krnjaja, Vesna, Zivković, Sanja, Marković, Jordan, Stević, Milan, "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 54, no. 12 (2019):964-971,
https://doi.org/10.1080/03601234.2019.1653735 . .

TY  - JOUR
AU  - Zivković, Sanja
AU  - Vasić, Tanja
AU  - Ivanović, Milan
AU  - Jevremović, Darko
AU  - Marković, Jordan
AU  - Trkulja, Vojislav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4958
AB  - During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Eutypa lata on grapevine in Serbia
EP  - 483
IS  - 5
SP  - 479
VL  - 126
DO  - 10.1007/s41348-019-00238-4
ER  - 

@article{
author = "Zivković, Sanja and Vasić, Tanja and Ivanović, Milan and Jevremović, Darko and Marković, Jordan and Trkulja, Vojislav",
year = "2019",
abstract = "During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Eutypa lata on grapevine in Serbia",
pages = "483-479",
number = "5",
volume = "126",
doi = "10.1007/s41348-019-00238-4"
}

Zivković, S., Vasić, T., Ivanović, M., Jevremović, D., Marković, J.,& Trkulja, V.. (2019). Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection
Springer Heidelberg, Heidelberg., 126(5), 479-483.
https://doi.org/10.1007/s41348-019-00238-4

Zivković S, Vasić T, Ivanović M, Jevremović D, Marković J, Trkulja V. Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection. 2019;126(5):479-483.
doi:10.1007/s41348-019-00238-4 .

Zivković, Sanja, Vasić, Tanja, Ivanović, Milan, Jevremović, Darko, Marković, Jordan, Trkulja, Vojislav, "Morphological and molecular identification of Eutypa lata on grapevine in Serbia" in Journal of Plant Diseases and Protection, 126, no. 5 (2019):479-483,
https://doi.org/10.1007/s41348-019-00238-4 . .