Dolovac, Nenad

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Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia

Gašić, Katarina; Gavrilović, Veljko; Dolovac, Nenad; Trkulja, Nenad; Živković, Svetlana; Ristić, Danijela; Obradović, Aleksa

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2014)

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3556
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 
@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}
Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G
Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .
Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .
12

First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia

Stevanović, M.; Stanković, Ivana; Vučurović, Ana; Dolovac, Nenad; Pfaf-Dolovac, Erika; Krstić, Branka; Bulajić, Aleksandra

(Amer Phytopathological Soc, St Paul, 2012)

TY  - JOUR
AU  - Stevanović, M.
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3019
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia
EP  - 912
IS  - 6
SP  - 912
VL  - 96
DO  - 10.1094/PDIS-02-12-0179-PDN
ER  - 
@article{
author = "Stevanović, M. and Stanković, Ivana and Vučurović, Ana and Dolovac, Nenad and Pfaf-Dolovac, Erika and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia",
pages = "912-912",
number = "6",
volume = "96",
doi = "10.1094/PDIS-02-12-0179-PDN"
}
Stevanović, M., Stanković, I., Vučurović, A., Dolovac, N., Pfaf-Dolovac, E., Krstić, B.,& Bulajić, A.. (2012). First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(6), 912-912.
https://doi.org/10.1094/PDIS-02-12-0179-PDN
Stevanović M, Stanković I, Vučurović A, Dolovac N, Pfaf-Dolovac E, Krstić B, Bulajić A. First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease. 2012;96(6):912-912.
doi:10.1094/PDIS-02-12-0179-PDN .
Stevanović, M., Stanković, Ivana, Vučurović, Ana, Dolovac, Nenad, Pfaf-Dolovac, Erika, Krstić, Branka, Bulajić, Aleksandra, "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia" in Plant Disease, 96, no. 6 (2012):912-912,
https://doi.org/10.1094/PDIS-02-12-0179-PDN . .
4

Influence of shading on the development of podosphaera leucotricha under field conditions

Miletić, Novica; Tamaš, Nenad; Vukša, Petar; Pfaf-Dolovac, Erika; Dolovac, Nenad

(2012)

TY  - JOUR
AU  - Miletić, Novica
AU  - Tamaš, Nenad
AU  - Vukša, Petar
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2825
AB  - The influence of shading on the incidence and development of P.leucotricha was examined at the locality of Belgrade, during 2008 and 2009. Investigation was carried out on two-year old apple nursery trees, cultivar Idared, sensitive to causal agent of apple powdery mildew. The experiment included three variants, two with different conditions of reduced light (24h and 48h shading, with 24h in the natural photoperiod) and the third variant, which included the cultivation of plants in natural photoperiod, without shading. Reduced light conditions were achieved by growing the plants under wooden experimental boxes. The boxes provided minimum differences in air temperature and RH values, inside and outside them. Inoculum was provided from two trees that were close to the experimental boxes, and the infection was carried out by direct application of conidia from infected young shoots and naturally, during growing period. Four assessments were made, three during shading period and one after removal of the experimental boxes. In both. years of investigation, the highest intensity of infection was manifested in the first variant (48h shading, 24h natural photoperiod), (14.0 - 100%), slightly lower in the second (24h shading, 24h natural photoperiod), (12.7 - 93.5%) and the lowest in variant of normal photoperiod, (0.1 - 16.6%). In shading variants, intensity of infection was rapidly increasing from the first to the third assessment, while in the fourth assessment, after the removal of boxes, it significantly decreased. The results of these studies indicate that light intensity significantly affects the appearance and development of P. leucotricha, and that apple powdery mildew develops intensively in the shade environmental conditions.
T2  - Bulgarian Journal of Agricultural Science
T1  - Influence of shading on the development of podosphaera leucotricha under field conditions
EP  - 184
IS  - 2
SP  - 178
VL  - 18
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2825
ER  - 
@article{
author = "Miletić, Novica and Tamaš, Nenad and Vukša, Petar and Pfaf-Dolovac, Erika and Dolovac, Nenad",
year = "2012",
abstract = "The influence of shading on the incidence and development of P.leucotricha was examined at the locality of Belgrade, during 2008 and 2009. Investigation was carried out on two-year old apple nursery trees, cultivar Idared, sensitive to causal agent of apple powdery mildew. The experiment included three variants, two with different conditions of reduced light (24h and 48h shading, with 24h in the natural photoperiod) and the third variant, which included the cultivation of plants in natural photoperiod, without shading. Reduced light conditions were achieved by growing the plants under wooden experimental boxes. The boxes provided minimum differences in air temperature and RH values, inside and outside them. Inoculum was provided from two trees that were close to the experimental boxes, and the infection was carried out by direct application of conidia from infected young shoots and naturally, during growing period. Four assessments were made, three during shading period and one after removal of the experimental boxes. In both. years of investigation, the highest intensity of infection was manifested in the first variant (48h shading, 24h natural photoperiod), (14.0 - 100%), slightly lower in the second (24h shading, 24h natural photoperiod), (12.7 - 93.5%) and the lowest in variant of normal photoperiod, (0.1 - 16.6%). In shading variants, intensity of infection was rapidly increasing from the first to the third assessment, while in the fourth assessment, after the removal of boxes, it significantly decreased. The results of these studies indicate that light intensity significantly affects the appearance and development of P. leucotricha, and that apple powdery mildew develops intensively in the shade environmental conditions.",
journal = "Bulgarian Journal of Agricultural Science",
title = "Influence of shading on the development of podosphaera leucotricha under field conditions",
pages = "184-178",
number = "2",
volume = "18",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2825"
}
Miletić, N., Tamaš, N., Vukša, P., Pfaf-Dolovac, E.,& Dolovac, N.. (2012). Influence of shading on the development of podosphaera leucotricha under field conditions. in Bulgarian Journal of Agricultural Science, 18(2), 178-184.
https://hdl.handle.net/21.15107/rcub_agrospace_2825
Miletić N, Tamaš N, Vukša P, Pfaf-Dolovac E, Dolovac N. Influence of shading on the development of podosphaera leucotricha under field conditions. in Bulgarian Journal of Agricultural Science. 2012;18(2):178-184.
https://hdl.handle.net/21.15107/rcub_agrospace_2825 .
Miletić, Novica, Tamaš, Nenad, Vukša, Petar, Pfaf-Dolovac, Erika, Dolovac, Nenad, "Influence of shading on the development of podosphaera leucotricha under field conditions" in Bulgarian Journal of Agricultural Science, 18, no. 2 (2012):178-184,
https://hdl.handle.net/21.15107/rcub_agrospace_2825 .
1
4

Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia

Dolovac, Nenad; Miletić, Novica; Trkulja, Nenad; Pfaf-Dolovac, Erika; Živković, Svetlana; Krstić, Branka; Bulajić, Aleksandra

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2011)

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2536
AB  - Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia.
AB  - Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia
T1  - Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 346
IS  - 4
SP  - 337
VL  - 26
DO  - 10.2298/PIF1104337D
ER  - 
@article{
author = "Dolovac, Nenad and Miletić, Novica and Trkulja, Nenad and Pfaf-Dolovac, Erika and Živković, Svetlana and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia., Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia, Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "346-337",
number = "4",
volume = "26",
doi = "10.2298/PIF1104337D"
}
Dolovac, N., Miletić, N., Trkulja, N., Pfaf-Dolovac, E., Živković, S., Krstić, B.,& Bulajić, A.. (2011). Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 337-346.
https://doi.org/10.2298/PIF1104337D
Dolovac N, Miletić N, Trkulja N, Pfaf-Dolovac E, Živković S, Krstić B, Bulajić A. Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina. 2011;26(4):337-346.
doi:10.2298/PIF1104337D .
Dolovac, Nenad, Miletić, Novica, Trkulja, Nenad, Pfaf-Dolovac, Erika, Živković, Svetlana, Krstić, Branka, Bulajić, Aleksandra, "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):337-346,
https://doi.org/10.2298/PIF1104337D . .

Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds

Poštić, Dobrivoj; Momirović, Nebojša; Broćić, Zoran; Dolijanović, Željko; Trkulja, Nenad; Dolovac, Nenad; Ivanović, Žarko

(Institut PKB Agroekonomik, Padinska skela, 2011)

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Momirović, Nebojša
AU  - Broćić, Zoran
AU  - Dolijanović, Željko
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Ivanović, Žarko
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2587
AB  - The aim of the study was to evaluate the quality of seeds of seven varieties of tomatoes. Average germination energy was 76%, while the average total germ inability was 85%. The lowest germination energy is achieved variety Merkati 65%, while the largest 85% reached variety Heinz 1370. Variety Heinz 1370 has achieved the highest total germinability of 93%. Health status of all investigated cultivars was correct and all values observed parameters the quality seeds are within the legally prescribed limits.
AB  - Cilj ispitivanja bio je ocena kvaliteta semena sedam sorti paradajza. Prosečna energija klijanja bila je 76%, dok je prosečna ukupna klijavost bila 85%. Najmanju energiju klijanja postigla je sorta Merkati 65%, dok je najveću postigla sorta Heinz 1370 85%. Sorta Heinz 1370 postigla je i najveću ukupnu klijavost od 93%. Zdravstveno stanje svih ispitivanih sorti bilo je ispravno, što zadovoljava zakonom propisane vrednosti.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds
T1  - Ocena kvaliteta semena paradajza (Lycopersicum esculentum L.)
EP  - 135
IS  - 1-2
SP  - 131
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2587
ER  - 
@article{
author = "Poštić, Dobrivoj and Momirović, Nebojša and Broćić, Zoran and Dolijanović, Željko and Trkulja, Nenad and Dolovac, Nenad and Ivanović, Žarko",
year = "2011",
abstract = "The aim of the study was to evaluate the quality of seeds of seven varieties of tomatoes. Average germination energy was 76%, while the average total germ inability was 85%. The lowest germination energy is achieved variety Merkati 65%, while the largest 85% reached variety Heinz 1370. Variety Heinz 1370 has achieved the highest total germinability of 93%. Health status of all investigated cultivars was correct and all values observed parameters the quality seeds are within the legally prescribed limits., Cilj ispitivanja bio je ocena kvaliteta semena sedam sorti paradajza. Prosečna energija klijanja bila je 76%, dok je prosečna ukupna klijavost bila 85%. Najmanju energiju klijanja postigla je sorta Merkati 65%, dok je najveću postigla sorta Heinz 1370 85%. Sorta Heinz 1370 postigla je i najveću ukupnu klijavost od 93%. Zdravstveno stanje svih ispitivanih sorti bilo je ispravno, što zadovoljava zakonom propisane vrednosti.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds, Ocena kvaliteta semena paradajza (Lycopersicum esculentum L.)",
pages = "135-131",
number = "1-2",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2587"
}
Poštić, D., Momirović, N., Broćić, Z., Dolijanović, Ž., Trkulja, N., Dolovac, N.,& Ivanović, Ž.. (2011). Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 17(1-2), 131-135.
https://hdl.handle.net/21.15107/rcub_agrospace_2587
Poštić D, Momirović N, Broćić Z, Dolijanović Ž, Trkulja N, Dolovac N, Ivanović Ž. Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2011;17(1-2):131-135.
https://hdl.handle.net/21.15107/rcub_agrospace_2587 .
Poštić, Dobrivoj, Momirović, Nebojša, Broćić, Zoran, Dolijanović, Željko, Trkulja, Nenad, Dolovac, Nenad, Ivanović, Žarko, "Evaluation of quality of tomato (Lycopersicum esculentum L.) seeds" in Zbornik naučnih radova Instituta PKB Agroekonomik, 17, no. 1-2 (2011):131-135,
https://hdl.handle.net/21.15107/rcub_agrospace_2587 .

Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha

Jankovics, Tuende; Dolovac, Nenad; Bulajić, Aleksandra; Krstić, Branka; Pascal, Thierry; Bardin, Marc; Nicot, Philippe C.; Kiss, Levente

(Amer Phytopathological Soc, St Paul, 2011)

TY  - JOUR
AU  - Jankovics, Tuende
AU  - Dolovac, Nenad
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Pascal, Thierry
AU  - Bardin, Marc
AU  - Nicot, Philippe C.
AU  - Kiss, Levente
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2709
AB  - Peach rusty spot, an economically important disease of peach (Prunus persica var. persica), appears as necrotic spots on fruit. The etiology of the disease is still not well understood, although it has long been suspected that the causal agent is the apple powdery mildew pathogen, Podosphaera leucotricha. This work confirmed this hypothesis based on cross-inoculation experiments and analysis of rDNA internal transcribed spacer sequences polymerase chain reaction amplified from rusty spot and peach powdery mildew lesions. Cross-inoculations of apple and peach leaves with P leucotricha and P. pannosa, the causal agent of peach powdery mildew, showed that (i) young peach fruit, up to 5 cm in diameter, developed symptoms typical of rusty spot following inoculation with P. leucotricha; (ii) leaves of 'Jonagold' apple seedlings developed powdery mildew infections when inoculated by touching young rusty spot lesions to their surfaces; (iii) P leucotricha sporulated on young peach fruit up to 5 cm in diameter; and (iv) peach leaves and young shoots were not susceptible to P leucotricha, whereas P. pannosa infected all the green parts of peach. A field experiment revealed that there was only a 2- to 3-week period of time during early peach fruit development when the epidermis was susceptible to P leucotricha. An outcome of this study is that now a clear distinction can be made between the symptoms caused by P pannosa and P leucotricha on peach.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha
EP  - 724
IS  - 6
SP  - 719
VL  - 95
DO  - 10.1094/PDIS-10-10-0711
ER  - 
@article{
author = "Jankovics, Tuende and Dolovac, Nenad and Bulajić, Aleksandra and Krstić, Branka and Pascal, Thierry and Bardin, Marc and Nicot, Philippe C. and Kiss, Levente",
year = "2011",
abstract = "Peach rusty spot, an economically important disease of peach (Prunus persica var. persica), appears as necrotic spots on fruit. The etiology of the disease is still not well understood, although it has long been suspected that the causal agent is the apple powdery mildew pathogen, Podosphaera leucotricha. This work confirmed this hypothesis based on cross-inoculation experiments and analysis of rDNA internal transcribed spacer sequences polymerase chain reaction amplified from rusty spot and peach powdery mildew lesions. Cross-inoculations of apple and peach leaves with P leucotricha and P. pannosa, the causal agent of peach powdery mildew, showed that (i) young peach fruit, up to 5 cm in diameter, developed symptoms typical of rusty spot following inoculation with P. leucotricha; (ii) leaves of 'Jonagold' apple seedlings developed powdery mildew infections when inoculated by touching young rusty spot lesions to their surfaces; (iii) P leucotricha sporulated on young peach fruit up to 5 cm in diameter; and (iv) peach leaves and young shoots were not susceptible to P leucotricha, whereas P. pannosa infected all the green parts of peach. A field experiment revealed that there was only a 2- to 3-week period of time during early peach fruit development when the epidermis was susceptible to P leucotricha. An outcome of this study is that now a clear distinction can be made between the symptoms caused by P pannosa and P leucotricha on peach.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha",
pages = "724-719",
number = "6",
volume = "95",
doi = "10.1094/PDIS-10-10-0711"
}
Jankovics, T., Dolovac, N., Bulajić, A., Krstić, B., Pascal, T., Bardin, M., Nicot, P. C.,& Kiss, L.. (2011). Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(6), 719-724.
https://doi.org/10.1094/PDIS-10-10-0711
Jankovics T, Dolovac N, Bulajić A, Krstić B, Pascal T, Bardin M, Nicot PC, Kiss L. Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha. in Plant Disease. 2011;95(6):719-724.
doi:10.1094/PDIS-10-10-0711 .
Jankovics, Tuende, Dolovac, Nenad, Bulajić, Aleksandra, Krstić, Branka, Pascal, Thierry, Bardin, Marc, Nicot, Philippe C., Kiss, Levente, "Peach Rusty Spot Is Caused by the Apple Powdery Mildew Fungus, Podosphaera leucotricha" in Plant Disease, 95, no. 6 (2011):719-724,
https://doi.org/10.1094/PDIS-10-10-0711 . .
6
8
8

Fungicide efficacy in peach rusty spot control in Serbia

Dolovac, Nenad; Miletić, Novica; Aleksić, Goran; Savić, Dušan; Živković, Svetlana; Trkulja, Nenad; Bulajić, Aleksandra

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2010)

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Aleksić, Goran
AU  - Savić, Dušan
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Bulajić, Aleksandra
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2250
AB  - Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures.
AB  - Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Fungicide efficacy in peach rusty spot control in Serbia
T1  - Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 249
IS  - 3
SP  - 241
VL  - 25
DO  - 10.2298/PIF1003241D
ER  - 
@article{
author = "Dolovac, Nenad and Miletić, Novica and Aleksić, Goran and Savić, Dušan and Živković, Svetlana and Trkulja, Nenad and Bulajić, Aleksandra",
year = "2010",
abstract = "Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures., Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Fungicide efficacy in peach rusty spot control in Serbia, Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "249-241",
number = "3",
volume = "25",
doi = "10.2298/PIF1003241D"
}
Dolovac, N., Miletić, N., Aleksić, G., Savić, D., Živković, S., Trkulja, N.,& Bulajić, A.. (2010). Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 241-249.
https://doi.org/10.2298/PIF1003241D
Dolovac N, Miletić N, Aleksić G, Savić D, Živković S, Trkulja N, Bulajić A. Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina. 2010;25(3):241-249.
doi:10.2298/PIF1003241D .
Dolovac, Nenad, Miletić, Novica, Aleksić, Goran, Savić, Dušan, Živković, Svetlana, Trkulja, Nenad, Bulajić, Aleksandra, "Fungicide efficacy in peach rusty spot control in Serbia" in Pesticidi i fitomedicina, 25, no. 3 (2010):241-249,
https://doi.org/10.2298/PIF1003241D . .
3