@conference{
author = "Vuković, Sandra and Moravčević, Đorđe and Jelačić, Slavica and Vujošević, Ana and Pećinar, Ilinka and Kilibarda, Sofija and Sudimac, Maja and Kostić, Aleksandar Ž.",
year = "2023",
abstract = "Myristica fragrans (fam. Myristicaceae) – the source of the spice nutmeg, is evergreen tree
widespread in warm and humid tropical climate. Indonesia is the largest nutmeg world
producer. Nutmeg seeds are valuable due many uses: in cooking (spice in sweet or salt dishes),
traditional medicine (diseases of the nervous, digestive and dentistry), additive in the products
of the pharmaceutical and perfumery industry. Nutmeg seed is a good source of primary
(carbohydrates, lipids, proteins, vitamins) and secondary metabolites (terpenes, phenolics,
organic acids). The main components are lipids (30-40%) and essential oil (10-15%). Scientific
sources indicate on many pharmacological effects of nutmeg: antidepressant, hepatoprotective,
antioxidant, anticancer, etc. Climatic conditions have significant impact on flavor and
therapeutic effect of nutmeg. In this research the content of total phenolics (TPC), total
flavonoids (TFC), total hydroxycinnamic acid derivatives (THCAs) and total carotenoids
(TCC) was determined by spectrophotometrically methods, in order to evaluate the potential
healing effect of nutmeg. Tested extracts were prepared from dried and ground nutmeg seed
commercially purchased, using two solvents (80% acetone and warm water - 50°C) and two
extraction techniques (solvent extraction (SE) and ultrasound assisted extraction (USE)). The
obtained results indicate a rich content of carotenoids and phenolics in acetone extracts.
Precisely, the TCC was 49.94 ± 0.70 μg/g in extract prepared by SE and 53.01 ± 1.28 μg/g in
extract prepared by UAE. The TPC in acetone extracts prepared by SE and UAE was similar,
28.93 ± 0.18 mg GAE/g of DW and 28.78 ± 0.07 mg GAE/g of DW, respectively, which is
statistically significantly higher than the TPC achieved in water extracts (2.97 ± 0.14 mg GAE/g
of DW for SE extract and 3.00 ± 0.21 mg GAE/g of DW for UAE extract). The TFC and THACs
achieved in acetone extracts was statistically significantly higher than TFC and THACs
observed in water extracts, where in the case of both solvents, the extraction technique does not
influence statistically significantly on the TF and THACs content. The TFC and THACs in
acetone extracts prepared by SE was 1.50 ± 0.06 mg CE/g of DW and 6.13 ± 0.08 mg CGAE/g
of DW, while acetone extracts prepared by UAE contained TF 1.59 ± 0.00 mg CE/g of DW and
THACs 5.96 ± 0.27 mg CGAE/g of DW, respectively.",
journal = "V. International Agricultural, Biological & Life Science Conference",
title = "Phytochemical properties of nutmeg (Myristica fragrans)",
pages = "260-260",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6841"
}