TY  - JOUR
AU  - Anicić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Pećinar, Ilinka
AU  - Brkusanin, Miloš
AU  - Nestorović-Zivković, Jasmina
AU  - Dmitrović, Slavica
AU  - Dajić-Stevanović, Zora
AU  - Schulz, Hartwig
AU  - Misić, Danijela
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4731
AB  - Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklic & Milojevic, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrISI. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants
EP  - 358
SP  - 347
VL  - 117
DO  - 10.1016/j.indcrop.2018.03.019
ER  - 

@article{
author = "Anicić, Neda and Matekalo, Dragana and Skorić, Marijana and Pećinar, Ilinka and Brkusanin, Miloš and Nestorović-Zivković, Jasmina and Dmitrović, Slavica and Dajić-Stevanović, Zora and Schulz, Hartwig and Misić, Danijela",
year = "2018",
abstract = "Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklic & Milojevic, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrISI. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants",
pages = "358-347",
volume = "117",
doi = "10.1016/j.indcrop.2018.03.019"
}

Anicić, N., Matekalo, D., Skorić, M., Pećinar, I., Brkusanin, M., Nestorović-Zivković, J., Dmitrović, S., Dajić-Stevanović, Z., Schulz, H.,& Misić, D.. (2018). Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 117, 347-358.
https://doi.org/10.1016/j.indcrop.2018.03.019

Anicić N, Matekalo D, Skorić M, Pećinar I, Brkusanin M, Nestorović-Zivković J, Dmitrović S, Dajić-Stevanović Z, Schulz H, Misić D. Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products. 2018;117:347-358.
doi:10.1016/j.indcrop.2018.03.019 .

Anicić, Neda, Matekalo, Dragana, Skorić, Marijana, Pećinar, Ilinka, Brkusanin, Miloš, Nestorović-Zivković, Jasmina, Dmitrović, Slavica, Dajić-Stevanović, Zora, Schulz, Hartwig, Misić, Danijela, "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants" in Industrial Crops and Products, 117 (2018):347-358,
https://doi.org/10.1016/j.indcrop.2018.03.019 . .