TY  - JOUR
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Nastasijević, Branislav
AU  - Antić-Mladenović, Svetlana
AU  - Vasić, Vesna
AU  - Matković, Ana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5022
AB  - Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m(-2)) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m(-2)), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m(-2) and 4.85 kg m(-2) of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models
EP  - 244
SP  - 236
VL  - 132
DO  - 10.1016/j.indcrop.2019.02.027
ER  - 

@article{
author = "Marković, Tatjana and Radanović, Dragoja and Nastasijević, Branislav and Antić-Mladenović, Svetlana and Vasić, Vesna and Matković, Ana",
year = "2019",
abstract = "Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m(-2)) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m(-2)), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m(-2) and 4.85 kg m(-2) of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models",
pages = "244-236",
volume = "132",
doi = "10.1016/j.indcrop.2019.02.027"
}

Marković, T., Radanović, D., Nastasijević, B., Antić-Mladenović, S., Vasić, V.,& Matković, A.. (2019). Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 132, 236-244.
https://doi.org/10.1016/j.indcrop.2019.02.027

Marković T, Radanović D, Nastasijević B, Antić-Mladenović S, Vasić V, Matković A. Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models. in Industrial Crops and Products. 2019;132:236-244.
doi:10.1016/j.indcrop.2019.02.027 .

Marković, Tatjana, Radanović, Dragoja, Nastasijević, Branislav, Antić-Mladenović, Svetlana, Vasić, Vesna, Matković, Ana, "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models" in Industrial Crops and Products, 132 (2019):236-244,
https://doi.org/10.1016/j.indcrop.2019.02.027 . .

TY  - JOUR
AU  - Čakar, Uroš D.
AU  - Grozdanić, Nada
AU  - Petrović, Aleksandar
AU  - Pejin, Boris
AU  - Nastasijević, Branislav
AU  - Marković, Bojan
AU  - Djordjević, Brizita
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4429
AB  - BACKGROUND: Fruit wines are well known for their profound health-promoting properties including both enzyme activations and inhibitions. They may act preventive in regard to diabetes melitus and other chronic diseases. Objectives: Potential alpha-glucosidase inhibitory activity of fruit wines made from blueberry, black chokeberry, blackberry, raspberry and sour cherry was the subject of this study. Method: In order to increase the alcohol content due to enriched extraction of total phenolics, sugar was added in the fruit pomace of the half of the examined fruit wine samples. Results: Compared with acarbose used as a positive control (IC50 = 73.78 mu g/mL), all fruit wine samples exhibited higher alpha-glucosidase inhibitory activity. Indeed, blueberry wine samples stood out, both prepared with IC50 = 24.14 mu g/mL, lyophilised extract yield 3.23% and without IC50 = 46.39 mu g/mL, lyophilised extract yield 2.89% and with addition of sugar before fermentation. Chlorogenic acid predominantly contributed to alpha-glucosidase inhibitory activity of the blueberry, black chokeberry and sour cherry wine samples. However, ellagic acid, a potent alpha-glucosidase inhibitor possessing a planar structure, only slightly affected the activity of the blueberry wine samples, due to the lower concentration. In addition to this, molecular docking study of chlorogenic acid pointed out the importance of binding energy (-8.5 kcal/mol) for the inhibition of the enzyme. Conclusion: In summary, fruit wines made from blueberry should be primarily taken into consideration as a medicinal food targeting diabetes mellitus type 2 in the early stage, if additional studies would confirm their therapeutic potential for the control of postprandial hyperglycemia.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Pharmaceutical Biotechnology
T1  - Fruit Wines Inhibitory Activity Against alpha-Glucosidase
EP  - 1272
IS  - 15
SP  - 1264
VL  - 18
DO  - 10.2174/1389201019666180410112439
ER  - 

@article{
author = "Čakar, Uroš D. and Grozdanić, Nada and Petrović, Aleksandar and Pejin, Boris and Nastasijević, Branislav and Marković, Bojan and Djordjević, Brizita",
year = "2017",
abstract = "BACKGROUND: Fruit wines are well known for their profound health-promoting properties including both enzyme activations and inhibitions. They may act preventive in regard to diabetes melitus and other chronic diseases. Objectives: Potential alpha-glucosidase inhibitory activity of fruit wines made from blueberry, black chokeberry, blackberry, raspberry and sour cherry was the subject of this study. Method: In order to increase the alcohol content due to enriched extraction of total phenolics, sugar was added in the fruit pomace of the half of the examined fruit wine samples. Results: Compared with acarbose used as a positive control (IC50 = 73.78 mu g/mL), all fruit wine samples exhibited higher alpha-glucosidase inhibitory activity. Indeed, blueberry wine samples stood out, both prepared with IC50 = 24.14 mu g/mL, lyophilised extract yield 3.23% and without IC50 = 46.39 mu g/mL, lyophilised extract yield 2.89% and with addition of sugar before fermentation. Chlorogenic acid predominantly contributed to alpha-glucosidase inhibitory activity of the blueberry, black chokeberry and sour cherry wine samples. However, ellagic acid, a potent alpha-glucosidase inhibitor possessing a planar structure, only slightly affected the activity of the blueberry wine samples, due to the lower concentration. In addition to this, molecular docking study of chlorogenic acid pointed out the importance of binding energy (-8.5 kcal/mol) for the inhibition of the enzyme. Conclusion: In summary, fruit wines made from blueberry should be primarily taken into consideration as a medicinal food targeting diabetes mellitus type 2 in the early stage, if additional studies would confirm their therapeutic potential for the control of postprandial hyperglycemia.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Pharmaceutical Biotechnology",
title = "Fruit Wines Inhibitory Activity Against alpha-Glucosidase",
pages = "1272-1264",
number = "15",
volume = "18",
doi = "10.2174/1389201019666180410112439"
}

Čakar, U. D., Grozdanić, N., Petrović, A., Pejin, B., Nastasijević, B., Marković, B.,& Djordjević, B.. (2017). Fruit Wines Inhibitory Activity Against alpha-Glucosidase. in Current Pharmaceutical Biotechnology
Bentham Science Publ Ltd, Sharjah., 18(15), 1264-1272.
https://doi.org/10.2174/1389201019666180410112439

Čakar UD, Grozdanić N, Petrović A, Pejin B, Nastasijević B, Marković B, Djordjević B. Fruit Wines Inhibitory Activity Against alpha-Glucosidase. in Current Pharmaceutical Biotechnology. 2017;18(15):1264-1272.
doi:10.2174/1389201019666180410112439 .

Čakar, Uroš D., Grozdanić, Nada, Petrović, Aleksandar, Pejin, Boris, Nastasijević, Branislav, Marković, Bojan, Djordjević, Brizita, "Fruit Wines Inhibitory Activity Against alpha-Glucosidase" in Current Pharmaceutical Biotechnology, 18, no. 15 (2017):1264-1272,
https://doi.org/10.2174/1389201019666180410112439 . .