TY  - JOUR
AU  - Savić, Milena D.
AU  - Klaus, Anita
AU  - Kozarski, Maja
AU  - Nikšić, Miomir
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2619
AB  - Shiitake (Lentinus edodes) belongs to medically important and delicious fungi. It is recognizable for its healing properties, excellent taste and rich aroma. According to the traditional Japanese and Chinese medicine, shiitake mushroom significantly increases the strength and vitality of the body. Shiitake contains immunostimulants, compounds that lower cholesterol, prevents clogging of blood vessels, regulates the pressure, balances blood sugar levels, regulates digestion, and improves the performance of respiratory organs by its antirheumatic and antiallergic activities. Shiitake is recommended to use as food, prevention and cure, usually in a form of a spice (dried and ground) or tea. It can be consumed fresh, too. The objective of this study was to test the effect of enrichment in selenium on antioxidant, reducing and free radical scavenging activity of water extracts from fruit body of Lentinus edodes. The fungus was enhanced by adding organic selenium, zinc (II) complex with the ligand 2.6-bis diacetylpyridine (selenosemicarbazon) and inorganic compounds (Na2SeO3) of selenium in nutritional substrate where the fungus was grown. The total selenium content in fruit body was around 50 ppm for the sample enriched with selenium originating from organic sources, and 80 ppm for the sample enriched with selenium from inorganic sources. Samples were prepared by extraction of fruiting bodies in heated water. The results indicated that water extracts of whole fruit bodies, from both control and mushrooms supplemented with selenium, had quite good antioxidant activity. However, there was no significant difference between the samples supplemented with selenium content and those that were not.
AB  - Shiitake (Lentinus edodes) pripada grupi medicinski značajnih i delikatesnih gljiva. Prepoznatljiva je po svojoj lekovitosti, izvanrednom ukusu i bogatoj aromi. Prema tradicionalnoj japanskoj i kineskoj medicini, gljiva shiitake značajno povećava snagu i vitalnost organizma. Shiitake sadrže imunostimulanse, sastojke koji snižavaju holesterol, sprečavaju začepljenje krvnih sudova, regulišu pritisak, uravnotežuju nivo šećera u krvi, regulišu probavu, poboljšavaju rad disajnih organa, deluju antireumatski i antialergijski. Preporuka je da se shiitake koriste kao ukusna hrana, preventiva i lek, najčešće kao začin (sušene i mlevene) ili čaj. Mogu se konzumirati i kao sveže pripremljene. Cilj rada bio je da se razjasni da li selen dodat u supstrat za gajenje gljive Lentinus edodes utiče na redukciona svojstva ekstrakta, antioksidativnu aktivnost ekstrakta, kao i procena reaktivnosti ekstrakata prema radikalskim vrstama. Gljiva je obogaćena selenom dodavanjem organskih, Zn (II) kompleks sa ligandom 2,6-diacetilpiridin bis (selenosemicarbazon), i neorganskih jedinjenja (Na2SeO3) selena u hranljivi supstrat na kojem je gljiva uzgajana. Ukupan sadržaj selena u plodonosnom telu kretao se oko 50 ppm za uzorak obogaćen selenom iz organskog izvora i 80 ppm za uzorak obogaćen selenom iz neorganskog izvora. Uzorci su pripremljeni ekstrakcijom plodonosnih tela u zagrejanoj vodi. Dobijeni rezultati ukazuju na to da vodeni ekstrakti celih gljiva, kako kontrolnih tako i sa dodatkom selena, imaju dobru antioksidativnu aktivnost. Međutim, nije primećena značajna razlika između uzoraka sa i bez sadržaja selena.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium
T1  - Antioksidativna aktivnost vodenog ekstrakta gljive Lentinus edodes obogaćene selenom
EP  - 314
IS  - 120
SP  - 307
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2619
ER  - 

@article{
author = "Savić, Milena D. and Klaus, Anita and Kozarski, Maja and Nikšić, Miomir",
year = "2011",
abstract = "Shiitake (Lentinus edodes) belongs to medically important and delicious fungi. It is recognizable for its healing properties, excellent taste and rich aroma. According to the traditional Japanese and Chinese medicine, shiitake mushroom significantly increases the strength and vitality of the body. Shiitake contains immunostimulants, compounds that lower cholesterol, prevents clogging of blood vessels, regulates the pressure, balances blood sugar levels, regulates digestion, and improves the performance of respiratory organs by its antirheumatic and antiallergic activities. Shiitake is recommended to use as food, prevention and cure, usually in a form of a spice (dried and ground) or tea. It can be consumed fresh, too. The objective of this study was to test the effect of enrichment in selenium on antioxidant, reducing and free radical scavenging activity of water extracts from fruit body of Lentinus edodes. The fungus was enhanced by adding organic selenium, zinc (II) complex with the ligand 2.6-bis diacetylpyridine (selenosemicarbazon) and inorganic compounds (Na2SeO3) of selenium in nutritional substrate where the fungus was grown. The total selenium content in fruit body was around 50 ppm for the sample enriched with selenium originating from organic sources, and 80 ppm for the sample enriched with selenium from inorganic sources. Samples were prepared by extraction of fruiting bodies in heated water. The results indicated that water extracts of whole fruit bodies, from both control and mushrooms supplemented with selenium, had quite good antioxidant activity. However, there was no significant difference between the samples supplemented with selenium content and those that were not., Shiitake (Lentinus edodes) pripada grupi medicinski značajnih i delikatesnih gljiva. Prepoznatljiva je po svojoj lekovitosti, izvanrednom ukusu i bogatoj aromi. Prema tradicionalnoj japanskoj i kineskoj medicini, gljiva shiitake značajno povećava snagu i vitalnost organizma. Shiitake sadrže imunostimulanse, sastojke koji snižavaju holesterol, sprečavaju začepljenje krvnih sudova, regulišu pritisak, uravnotežuju nivo šećera u krvi, regulišu probavu, poboljšavaju rad disajnih organa, deluju antireumatski i antialergijski. Preporuka je da se shiitake koriste kao ukusna hrana, preventiva i lek, najčešće kao začin (sušene i mlevene) ili čaj. Mogu se konzumirati i kao sveže pripremljene. Cilj rada bio je da se razjasni da li selen dodat u supstrat za gajenje gljive Lentinus edodes utiče na redukciona svojstva ekstrakta, antioksidativnu aktivnost ekstrakta, kao i procena reaktivnosti ekstrakata prema radikalskim vrstama. Gljiva je obogaćena selenom dodavanjem organskih, Zn (II) kompleks sa ligandom 2,6-diacetilpiridin bis (selenosemicarbazon), i neorganskih jedinjenja (Na2SeO3) selena u hranljivi supstrat na kojem je gljiva uzgajana. Ukupan sadržaj selena u plodonosnom telu kretao se oko 50 ppm za uzorak obogaćen selenom iz organskog izvora i 80 ppm za uzorak obogaćen selenom iz neorganskog izvora. Uzorci su pripremljeni ekstrakcijom plodonosnih tela u zagrejanoj vodi. Dobijeni rezultati ukazuju na to da vodeni ekstrakti celih gljiva, kako kontrolnih tako i sa dodatkom selena, imaju dobru antioksidativnu aktivnost. Međutim, nije primećena značajna razlika između uzoraka sa i bez sadržaja selena.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium, Antioksidativna aktivnost vodenog ekstrakta gljive Lentinus edodes obogaćene selenom",
pages = "314-307",
number = "120",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2619"
}

Savić, M. D., Klaus, A., Kozarski, M.,& Nikšić, M.. (2011). Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 307-314.
https://hdl.handle.net/21.15107/rcub_agrospace_2619

Savić MD, Klaus A, Kozarski M, Nikšić M. Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium. in Zbornik Matice srpske za prirodne nauke. 2011;(120):307-314.
https://hdl.handle.net/21.15107/rcub_agrospace_2619 .

Savić, Milena D., Klaus, Anita, Kozarski, Maja, Nikšić, Miomir, "Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):307-314,
https://hdl.handle.net/21.15107/rcub_agrospace_2619 .

TY  - JOUR
AU  - Savić, Milena D.
AU  - Petrović, Jelena
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Rajković, Miloš
AU  - Filipović, Nenad
AU  - Antić-Mladenović, Svetlana
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2040
AB  - Selenium is a trace mineral chemically related to sulfur and tellurium. In the body selenium combines with protein molecules to form selenoproteins and it is distributed in low concentrations and unequally in air, soil and water all over the world. Edible mushrooms are known to be selenium accumulators. Since mushrooms contain relatively high protein levels, and they can accumulate large amounts of selenium, it is reasonable to expect that selenium could be incorporated into proteins. The growth of mycelia and fruit body formation of different medicinal mushroom strains of Pleurotus ostreatus (Hk-35 and P70) over the wide range of concentrations of inorganic form of selenium were examined. Mushrooms were cultivated on agar base media and on substrates based on sawdust. Vegetative growths of mycelium were measured as colony diameter in pure cultures supplemented with inorganic form of Se supplements, prepared as Na2SeO4 and Na2SeO3 in concentrations of: 1, 10, 25, 50, 75, 100 and 150 mg/l. Inorganic form of Se supplements, showed stimulation effects (in concentration of 1-50 mg/l) and toxic effects in higher concentration. On the standard industrial sawdust based substrate, supplemented with 100 mg/kg Na2SeO4 and Na2SeO3, accumulation of Se in fruit bodies was determined by the method of flameless atomic absorption spectrophotometer. The readings were performed on Varian SpectrAA-10 spectrophotometer equipped with VGA-76. Se as Na2SeO4 and Na2SeO3 was effectively taken up from substrates and accumulated in fruit bodies. Mushrooms accumulated selenium between 120 and 250 mg/kg dry weight. In mushrooms cultivated without Se supplement, Se contents were only about 1 mg/kg and in substrate about 0.1 mg/kg.
AB  - Selen je esencijelni mikroelement, neophodan u malim količinama. Zemljište na teritoriji Evrope ima nizak sadržaj selena. Pečurke sadrže relativno visok nivo proteina, te stoga mogu da usvoje visoke koncentracije selena, koji se inkorporira u proteine formirajući selenoproteine. U radu je ispitivan uticaj većeg broja različitih koncentracija neorganskih jedinjenja selena na porast i formiranje plodonosnih tela medicinski značajne gljive Pleurotus ostreatus (komercijalni sojevi Hk-35 i R70). Porast micelijuma na sladnom agaru praćen je merenjem prečnika kolonije čiste kulture obogaćene neorganskim jedinjenjima selena. Korišćena neorganska jedinjenja selena, u obliku Na2SeO4 i Na2SeO3 (1-50 mg/l), stimulisala su porast micelijuma, dok su veće koncentracije pokazale različit toksičan efekat u zavisnosti od koncentracije dodavanog jedinjenja i soja gljive. Pečurke su zatim gajene na hranljivom supstratu obogaćenom neorganskim jedinjenjima selena koncentracije do 100 mg/kg. Sadržaj selena u plodonosnim telima određen je pomoću AAS (hidridni metod) nakon vlažne digestije i rezultati su očitani na VarianSpectarAA-10 spektrofotometru sa VGA-76 (pomoćni aparat za isparavanje) LSD testom. Analize su pokazale da su gljive upešno usvojile selen u plodonosnom telu. Totalni sadržaj selena u plodonosnom telu pečuraka zavisio je od ispitivanog soja i vrste dodavanog jedinjenja. Pleurotus ostreatus je bolje usvojila selen iz Na2SeO3 nego iz Na2SeO4. Koncentracija usvojenog selena se kretala između 120 i 250 mg/kg suve mase. U pečurkama koje su gajene na supstratu bez dodatka selena, sadržaj selena je iznosio svega oko 1 μg/g, dok se ova vrednost za čist supstrat kretala oko 0.1 mg/kg.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Growth and fruit body formation of Pleurotus ostreatus on media supplemented with inorganic selenium
T1  - Porast i formiranje plodonosnih tela gljive Pleurotus ostreatus na supstratu obogaćenom neorganskim jedinjenjima selena
EP  - 215
IS  - 116
SP  - 209
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2040
ER  - 

@article{
author = "Savić, Milena D. and Petrović, Jelena and Klaus, Anita and Nikšić, Miomir and Rajković, Miloš and Filipović, Nenad and Antić-Mladenović, Svetlana",
year = "2009",
abstract = "Selenium is a trace mineral chemically related to sulfur and tellurium. In the body selenium combines with protein molecules to form selenoproteins and it is distributed in low concentrations and unequally in air, soil and water all over the world. Edible mushrooms are known to be selenium accumulators. Since mushrooms contain relatively high protein levels, and they can accumulate large amounts of selenium, it is reasonable to expect that selenium could be incorporated into proteins. The growth of mycelia and fruit body formation of different medicinal mushroom strains of Pleurotus ostreatus (Hk-35 and P70) over the wide range of concentrations of inorganic form of selenium were examined. Mushrooms were cultivated on agar base media and on substrates based on sawdust. Vegetative growths of mycelium were measured as colony diameter in pure cultures supplemented with inorganic form of Se supplements, prepared as Na2SeO4 and Na2SeO3 in concentrations of: 1, 10, 25, 50, 75, 100 and 150 mg/l. Inorganic form of Se supplements, showed stimulation effects (in concentration of 1-50 mg/l) and toxic effects in higher concentration. On the standard industrial sawdust based substrate, supplemented with 100 mg/kg Na2SeO4 and Na2SeO3, accumulation of Se in fruit bodies was determined by the method of flameless atomic absorption spectrophotometer. The readings were performed on Varian SpectrAA-10 spectrophotometer equipped with VGA-76. Se as Na2SeO4 and Na2SeO3 was effectively taken up from substrates and accumulated in fruit bodies. Mushrooms accumulated selenium between 120 and 250 mg/kg dry weight. In mushrooms cultivated without Se supplement, Se contents were only about 1 mg/kg and in substrate about 0.1 mg/kg., Selen je esencijelni mikroelement, neophodan u malim količinama. Zemljište na teritoriji Evrope ima nizak sadržaj selena. Pečurke sadrže relativno visok nivo proteina, te stoga mogu da usvoje visoke koncentracije selena, koji se inkorporira u proteine formirajući selenoproteine. U radu je ispitivan uticaj većeg broja različitih koncentracija neorganskih jedinjenja selena na porast i formiranje plodonosnih tela medicinski značajne gljive Pleurotus ostreatus (komercijalni sojevi Hk-35 i R70). Porast micelijuma na sladnom agaru praćen je merenjem prečnika kolonije čiste kulture obogaćene neorganskim jedinjenjima selena. Korišćena neorganska jedinjenja selena, u obliku Na2SeO4 i Na2SeO3 (1-50 mg/l), stimulisala su porast micelijuma, dok su veće koncentracije pokazale različit toksičan efekat u zavisnosti od koncentracije dodavanog jedinjenja i soja gljive. Pečurke su zatim gajene na hranljivom supstratu obogaćenom neorganskim jedinjenjima selena koncentracije do 100 mg/kg. Sadržaj selena u plodonosnim telima određen je pomoću AAS (hidridni metod) nakon vlažne digestije i rezultati su očitani na VarianSpectarAA-10 spektrofotometru sa VGA-76 (pomoćni aparat za isparavanje) LSD testom. Analize su pokazale da su gljive upešno usvojile selen u plodonosnom telu. Totalni sadržaj selena u plodonosnom telu pečuraka zavisio je od ispitivanog soja i vrste dodavanog jedinjenja. Pleurotus ostreatus je bolje usvojila selen iz Na2SeO3 nego iz Na2SeO4. Koncentracija usvojenog selena se kretala između 120 i 250 mg/kg suve mase. U pečurkama koje su gajene na supstratu bez dodatka selena, sadržaj selena je iznosio svega oko 1 μg/g, dok se ova vrednost za čist supstrat kretala oko 0.1 mg/kg.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Growth and fruit body formation of Pleurotus ostreatus on media supplemented with inorganic selenium, Porast i formiranje plodonosnih tela gljive Pleurotus ostreatus na supstratu obogaćenom neorganskim jedinjenjima selena",
pages = "215-209",
number = "116",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2040"
}

Savić, M. D., Petrović, J., Klaus, A., Nikšić, M., Rajković, M., Filipović, N.,& Antić-Mladenović, S.. (2009). Growth and fruit body formation of Pleurotus ostreatus on media supplemented with inorganic selenium. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 209-215.
https://hdl.handle.net/21.15107/rcub_agrospace_2040

Savić MD, Petrović J, Klaus A, Nikšić M, Rajković M, Filipović N, Antić-Mladenović S. Growth and fruit body formation of Pleurotus ostreatus on media supplemented with inorganic selenium. in Zbornik Matice srpske za prirodne nauke. 2009;(116):209-215.
https://hdl.handle.net/21.15107/rcub_agrospace_2040 .

Savić, Milena D., Petrović, Jelena, Klaus, Anita, Nikšić, Miomir, Rajković, Miloš, Filipović, Nenad, Antić-Mladenović, Svetlana, "Growth and fruit body formation of Pleurotus ostreatus on media supplemented with inorganic selenium" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):209-215,
https://hdl.handle.net/21.15107/rcub_agrospace_2040 .