TY  - JOUR
AU  - Vasić, Tanja
AU  - Vojinović, Uroš
AU  - Zujović, Suzana
AU  - Krnjaja, Vesna
AU  - Zivković, Sanja
AU  - Marković, Jordan
AU  - Stević, Milan
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4954
AB  - Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
T1  - In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum
EP  - 971
IS  - 12
SP  - 964
VL  - 54
DO  - 10.1080/03601234.2019.1653735
ER  - 

@article{
author = "Vasić, Tanja and Vojinović, Uroš and Zujović, Suzana and Krnjaja, Vesna and Zivković, Sanja and Marković, Jordan and Stević, Milan",
year = "2019",
abstract = "Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was",
title = "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum",
pages = "971-964",
number = "12",
volume = "54",
doi = "10.1080/03601234.2019.1653735"
}

Vasić, T., Vojinović, U., Zujović, S., Krnjaja, V., Zivković, S., Marković, J.,& Stević, M.. (2019). In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
Taylor & Francis Inc, Philadelphia., 54(12), 964-971.
https://doi.org/10.1080/03601234.2019.1653735

Vasić T, Vojinović U, Zujović S, Krnjaja V, Zivković S, Marković J, Stević M. In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2019;54(12):964-971.
doi:10.1080/03601234.2019.1653735 .

Vasić, Tanja, Vojinović, Uroš, Zujović, Suzana, Krnjaja, Vesna, Zivković, Sanja, Marković, Jordan, Stević, Milan, "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 54, no. 12 (2019):964-971,
https://doi.org/10.1080/03601234.2019.1653735 . .

TY  - JOUR
AU  - Zivković, Sanja
AU  - Vasić, Tanja
AU  - Ivanović, Milan
AU  - Jevremović, Darko
AU  - Marković, Jordan
AU  - Trkulja, Vojislav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4958
AB  - During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Eutypa lata on grapevine in Serbia
EP  - 483
IS  - 5
SP  - 479
VL  - 126
DO  - 10.1007/s41348-019-00238-4
ER  - 

@article{
author = "Zivković, Sanja and Vasić, Tanja and Ivanović, Milan and Jevremović, Darko and Marković, Jordan and Trkulja, Vojislav",
year = "2019",
abstract = "During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Eutypa lata on grapevine in Serbia",
pages = "483-479",
number = "5",
volume = "126",
doi = "10.1007/s41348-019-00238-4"
}

Zivković, S., Vasić, T., Ivanović, M., Jevremović, D., Marković, J.,& Trkulja, V.. (2019). Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection
Springer Heidelberg, Heidelberg., 126(5), 479-483.
https://doi.org/10.1007/s41348-019-00238-4

Zivković S, Vasić T, Ivanović M, Jevremović D, Marković J, Trkulja V. Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection. 2019;126(5):479-483.
doi:10.1007/s41348-019-00238-4 .

Zivković, Sanja, Vasić, Tanja, Ivanović, Milan, Jevremović, Darko, Marković, Jordan, Trkulja, Vojislav, "Morphological and molecular identification of Eutypa lata on grapevine in Serbia" in Journal of Plant Diseases and Protection, 126, no. 5 (2019):479-483,
https://doi.org/10.1007/s41348-019-00238-4 . .

TY  - JOUR
AU  - Blagojević, Milomir
AU  - Djordjević, Nenad
AU  - Dinić, Bora
AU  - Vasić, Tanja
AU  - Milenković, Jasmina
AU  - Petrović, Mirjana
AU  - Marković, Jordan
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4423
AB  - This research was conducted to determine the effect of seed rates in mixtures of pea + oat on the green forage and silage protein fractions evaluated by Cornell net carbohydrate and protein system (CNCPS). Experiment was established in autumn of 2012, on October the 20th and plant samples were taken in spring 2013 at forming the first pods on 2/3 plants of pea at Institute for forage crops, Krusevac, Republic of Serbia, using five different mixture rates of pea and oat crops (100% pea + 0% oat; 0% pea + 100% oat; 25% pea + 75% oat; 50% pea + 50% oat and 75% pea + 25% oat). After harvesting pea: oat mixtures were treated with bacterial inoculant and ensiled in anaerobic jars for 45 days. Green forages and silage samples were analyzed for DM (dry matter), CP (crude protein), primary protein fractions-TP (true protein), NPN (non protein nitrogen), IP (insoluble protein), SolP (soluble protein), NDICP (neutral detergent insoluble crude protein) and ADICP (acid detergent insoluble crude protein) and protein fractions by CNCPS. An analysis of variance found statistically significant differences among mixture rates for all variables, except IP and SolP. Silage from monoculture pea had the highest NPN (696.2 g kg(-1) CP) and SolP (713.8 g kg(-1) CP), followed by the mixture of pea with oat 75:25 (662.5 and 653.4 g kg(-1) CP, respectively). Analyzing the CNCPS protein fractions of pea: oat silages it was found that silage from pea monoculture contained the highest PA fraction (non protein nitrogen, immediately degraded in the rumen) and that PA fraction increased with increasing pea ratios in silages, which was a direct reflection of their high NPN and SolP in green forages and in silages. Because of those facts 25:50 and 50:50 pea: oat mixtures silages could be recommended for ruminant feeding. The investigated bacterial inoculant can increase the TP content, as well as PB 1 (true protein rapidly degraded in the rumen) and PB 3 (slowly degraded True Protein in the rumen, because it is associated with the cell wall) fractions.
PB  - Ankara Univ, Fac Agr, Diskapi
T2  - Journal of Agricultural Sciences-Tarim Bilimleri Dergisi
T1  - Determination of Green Forage and Silage Protein Degradability of Some Pea (Pisum sativum L.) plus Oat (Avena sativa L.) Mixtures Grown in Serbia
EP  - 422
IS  - 4
SP  - 415
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4423
ER  - 

@article{
author = "Blagojević, Milomir and Djordjević, Nenad and Dinić, Bora and Vasić, Tanja and Milenković, Jasmina and Petrović, Mirjana and Marković, Jordan",
year = "2017",
abstract = "This research was conducted to determine the effect of seed rates in mixtures of pea + oat on the green forage and silage protein fractions evaluated by Cornell net carbohydrate and protein system (CNCPS). Experiment was established in autumn of 2012, on October the 20th and plant samples were taken in spring 2013 at forming the first pods on 2/3 plants of pea at Institute for forage crops, Krusevac, Republic of Serbia, using five different mixture rates of pea and oat crops (100% pea + 0% oat; 0% pea + 100% oat; 25% pea + 75% oat; 50% pea + 50% oat and 75% pea + 25% oat). After harvesting pea: oat mixtures were treated with bacterial inoculant and ensiled in anaerobic jars for 45 days. Green forages and silage samples were analyzed for DM (dry matter), CP (crude protein), primary protein fractions-TP (true protein), NPN (non protein nitrogen), IP (insoluble protein), SolP (soluble protein), NDICP (neutral detergent insoluble crude protein) and ADICP (acid detergent insoluble crude protein) and protein fractions by CNCPS. An analysis of variance found statistically significant differences among mixture rates for all variables, except IP and SolP. Silage from monoculture pea had the highest NPN (696.2 g kg(-1) CP) and SolP (713.8 g kg(-1) CP), followed by the mixture of pea with oat 75:25 (662.5 and 653.4 g kg(-1) CP, respectively). Analyzing the CNCPS protein fractions of pea: oat silages it was found that silage from pea monoculture contained the highest PA fraction (non protein nitrogen, immediately degraded in the rumen) and that PA fraction increased with increasing pea ratios in silages, which was a direct reflection of their high NPN and SolP in green forages and in silages. Because of those facts 25:50 and 50:50 pea: oat mixtures silages could be recommended for ruminant feeding. The investigated bacterial inoculant can increase the TP content, as well as PB 1 (true protein rapidly degraded in the rumen) and PB 3 (slowly degraded True Protein in the rumen, because it is associated with the cell wall) fractions.",
publisher = "Ankara Univ, Fac Agr, Diskapi",
journal = "Journal of Agricultural Sciences-Tarim Bilimleri Dergisi",
title = "Determination of Green Forage and Silage Protein Degradability of Some Pea (Pisum sativum L.) plus Oat (Avena sativa L.) Mixtures Grown in Serbia",
pages = "422-415",
number = "4",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4423"
}

Blagojević, M., Djordjević, N., Dinić, B., Vasić, T., Milenković, J., Petrović, M.,& Marković, J.. (2017). Determination of Green Forage and Silage Protein Degradability of Some Pea (Pisum sativum L.) plus Oat (Avena sativa L.) Mixtures Grown in Serbia. in Journal of Agricultural Sciences-Tarim Bilimleri Dergisi
Ankara Univ, Fac Agr, Diskapi., 23(4), 415-422.
https://hdl.handle.net/21.15107/rcub_agrospace_4423

Blagojević M, Djordjević N, Dinić B, Vasić T, Milenković J, Petrović M, Marković J. Determination of Green Forage and Silage Protein Degradability of Some Pea (Pisum sativum L.) plus Oat (Avena sativa L.) Mixtures Grown in Serbia. in Journal of Agricultural Sciences-Tarim Bilimleri Dergisi. 2017;23(4):415-422.
https://hdl.handle.net/21.15107/rcub_agrospace_4423 .

Blagojević, Milomir, Djordjević, Nenad, Dinić, Bora, Vasić, Tanja, Milenković, Jasmina, Petrović, Mirjana, Marković, Jordan, "Determination of Green Forage and Silage Protein Degradability of Some Pea (Pisum sativum L.) plus Oat (Avena sativa L.) Mixtures Grown in Serbia" in Journal of Agricultural Sciences-Tarim Bilimleri Dergisi, 23, no. 4 (2017):415-422,
https://hdl.handle.net/21.15107/rcub_agrospace_4423 .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, Tanja
AU  - Vasić, Tanja
AU  - Obradović, Ana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3769
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
EP  - 256
SP  - 251
VL  - 67
DO  - 10.1016/j.cropro.2014.11.001
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Petrović, Tanja and Vasić, Tanja and Obradović, Ana",
year = "2015",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
pages = "256-251",
volume = "67",
doi = "10.1016/j.cropro.2014.11.001"
}

Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Petrović, T., Vasić, T.,& Obradović, A.. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001

Krnjaja V, Mandić V, Lević J, Stanković S, Petrović T, Vasić T, Obradović A. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. in Crop Protection. 2015;67:251-256.
doi:10.1016/j.cropro.2014.11.001 .

Krnjaja, Vesna, Mandić, Violeta, Lević, Jelena, Stanković, Slavica, Petrović, Tanja, Vasić, Tanja, Obradović, Ana, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" in Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 . .