TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4826
AB  - The number of virus species infecting grapevine has increased continuously and more than 60 species are described to infect grapevine. The global expansion of the grape and wine industry has seen a parallel increase in the incidence and economic impact of grapevine leafroll disease (GLD). GLD can cause reduced plant vigor and longevity, and significant losses in both fruit yield and quality. Our knowledge of the nature of the disease is still quite limited due to a variety of challenges related to the complexity of this virus disease, the association of several genetically and serologically distinct GLRaVs, and contrasting symptoms in red- and white-berried cultivars. Within grapevine leafroll complex (Grapevine leafroll-associated viruses, GLRaVs) five different species are classified in the family Closteroviridae: one is classified in the genus Closterovirus (GLRaV-2), three in the genus Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, and his strains: GLRaV-4 strain 5, GLRaV-4 strain 6, GLRaV-4 strain 9, GLRaV-4 strain Pr, GLRaV-4 strain De and GLRaV-4 strain Car) and one in the genus Velarivirus (GLRaV-7). GLRaVs are transmitted by grafting and propagation material (rootstock and scion) and some species by mealybugs. Grapevine leafroll-associated viruses usually occur in a mixed infection, and are often present in a mixed infection with other grape viruses. Early research carried out in vineyards in Serbia determined the presence of GLRV-1, -2 and -3, with GLRaV-3 being the most prevalent. If spread of the viruses transsmited by vectors within plantings exists, roguing or removal of infected vines, mealybug control, and sanitation measures can reduce new infections.
AB  - Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Grapevine Leafroll complex
T1  - Kompleks virusa prouzrokovača uvijenosti lišća vinove loze
EP  - 690
IS  - 6
SP  - 681
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4826
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Krstić, Branka",
year = "2018",
abstract = "The number of virus species infecting grapevine has increased continuously and more than 60 species are described to infect grapevine. The global expansion of the grape and wine industry has seen a parallel increase in the incidence and economic impact of grapevine leafroll disease (GLD). GLD can cause reduced plant vigor and longevity, and significant losses in both fruit yield and quality. Our knowledge of the nature of the disease is still quite limited due to a variety of challenges related to the complexity of this virus disease, the association of several genetically and serologically distinct GLRaVs, and contrasting symptoms in red- and white-berried cultivars. Within grapevine leafroll complex (Grapevine leafroll-associated viruses, GLRaVs) five different species are classified in the family Closteroviridae: one is classified in the genus Closterovirus (GLRaV-2), three in the genus Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, and his strains: GLRaV-4 strain 5, GLRaV-4 strain 6, GLRaV-4 strain 9, GLRaV-4 strain Pr, GLRaV-4 strain De and GLRaV-4 strain Car) and one in the genus Velarivirus (GLRaV-7). GLRaVs are transmitted by grafting and propagation material (rootstock and scion) and some species by mealybugs. Grapevine leafroll-associated viruses usually occur in a mixed infection, and are often present in a mixed infection with other grape viruses. Early research carried out in vineyards in Serbia determined the presence of GLRV-1, -2 and -3, with GLRaV-3 being the most prevalent. If spread of the viruses transsmited by vectors within plantings exists, roguing or removal of infected vines, mealybug control, and sanitation measures can reduce new infections., Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Grapevine Leafroll complex, Kompleks virusa prouzrokovača uvijenosti lišća vinove loze",
pages = "690-681",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4826"
}

Ristić, D., Vučurović, I., Stanković, I., Vučurović, A., Zečević, K.,& Krstić, B.. (2018). Grapevine Leafroll complex. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 681-690.
https://hdl.handle.net/21.15107/rcub_agrospace_4826

Ristić D, Vučurović I, Stanković I, Vučurović A, Zečević K, Krstić B. Grapevine Leafroll complex. in Biljni lekar. 2018;46(6):681-690.
https://hdl.handle.net/21.15107/rcub_agrospace_4826 .

Ristić, Danijela, Vučurović, Ivan, Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Krstić, Branka, "Grapevine Leafroll complex" in Biljni lekar, 46, no. 6 (2018):681-690,
https://hdl.handle.net/21.15107/rcub_agrospace_4826 .

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Nikolić, Dušan
AU  - Radović, Nikola
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4603
AB  - Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
AB  - Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Incidence and distribution of leek yellow stripe virus in allium crops in Serbia
T1  - Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji
EP  - 155
IS  - 3-4
SP  - 145
VL  - 32
DO  - 10.2298/PIF1704145V
ER  - 

@article{
author = "Vučurović, Ivan and Nikolić, Dušan and Radović, Nikola and Vučurović, Ana and Ristić, Danijela and Krstić, Branka and Stanković, Ivana",
year = "2017",
abstract = "Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E., Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia, Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji",
pages = "155-145",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704145V"
}

Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B.,& Stanković, I.. (2017). Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 32(3-4), 145-155.
https://doi.org/10.2298/PIF1704145V

Vučurović I, Nikolić D, Radović N, Vučurović A, Ristić D, Krstić B, Stanković I. Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):145-155.
doi:10.2298/PIF1704145V .

Vučurović, Ivan, Nikolić, Dušan, Radović, Nikola, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, Stanković, Ivana, "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):145-155,
https://doi.org/10.2298/PIF1704145V . .