TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Mutavdžić, Dragosav
AU  - Srećković, Tatjana
AU  - Veljović Jovanović, Sonja
AU  - Prokić, Ljiljana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5959
AB  - Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.
PB  - MDPI
T2  - Plants
T1  - Differential response of two tomato genotypes, wild type cv. Ailsa craig and its aba-deficient mutant flacca to short-termed drought cycles
IS  - 11
SP  - 2308
VL  - 10
DO  - 10.3390/plants10112308
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Nikolić, Nenad and Mutavdžić, Dragosav and Srećković, Tatjana and Veljović Jovanović, Sonja and Prokić, Ljiljana",
year = "2021",
abstract = "Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.",
publisher = "MDPI",
journal = "Plants",
title = "Differential response of two tomato genotypes, wild type cv. Ailsa craig and its aba-deficient mutant flacca to short-termed drought cycles",
number = "11",
pages = "2308",
volume = "10",
doi = "10.3390/plants10112308"
}

Živanović, B., Milić Komić, S., Nikolić, N., Mutavdžić, D., Srećković, T., Veljović Jovanović, S.,& Prokić, L.. (2021). Differential response of two tomato genotypes, wild type cv. Ailsa craig and its aba-deficient mutant flacca to short-termed drought cycles. in Plants
MDPI., 10(11), 2308.
https://doi.org/10.3390/plants10112308

Živanović B, Milić Komić S, Nikolić N, Mutavdžić D, Srećković T, Veljović Jovanović S, Prokić L. Differential response of two tomato genotypes, wild type cv. Ailsa craig and its aba-deficient mutant flacca to short-termed drought cycles. in Plants. 2021;10(11):2308.
doi:10.3390/plants10112308 .

Živanović, Bojana, Milić Komić, Sonja, Nikolić, Nenad, Mutavdžić, Dragosav, Srećković, Tatjana, Veljović Jovanović, Sonja, Prokić, Ljiljana, "Differential response of two tomato genotypes, wild type cv. Ailsa craig and its aba-deficient mutant flacca to short-termed drought cycles" in Plants, 10, no. 11 (2021):2308,
https://doi.org/10.3390/plants10112308 . .

TY  - JOUR
AU  - Zivanović, Bojana
AU  - Milić-Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokić, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5284
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI, BASEL
T2  - Plants-Basel
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
VL  - 9
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Zivanović, Bojana and Milić-Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokić, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI, BASEL",
journal = "Plants-Basel",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
volume = "9",
doi = "10.3390/plants9091147"
}

Zivanović, B., Milić-Komić, S., Tosti, T., Vidović, M., Prokić, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel
MDPI, BASEL., 9(9).
https://doi.org/10.3390/plants9091147

Zivanović B, Milić-Komić S, Tosti T, Vidović M, Prokić L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel. 2020;9(9).
doi:10.3390/plants9091147 .

Zivanović, Bojana, Milić-Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokić, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants-Basel, 9, no. 9 (2020),
https://doi.org/10.3390/plants9091147 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Prokić, Ljiljana
AU  - Milić-Komić, Sonja
AU  - Zivanović, Bojana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4068
AB  - In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat
EP  - 39
SP  - 25
VL  - 206
DO  - 10.1016/j.jplph.2016.07.017
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Prokić, Ljiljana and Milić-Komić, Sonja and Zivanović, Bojana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat",
pages = "39-25",
volume = "206",
doi = "10.1016/j.jplph.2016.07.017"
}

Vidović, M., Morina, F., Prokić, L., Milić-Komić, S., Zivanović, B.,& Veljović-Jovanović, S.. (2016). Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 206, 25-39.
https://doi.org/10.1016/j.jplph.2016.07.017

Vidović M, Morina F, Prokić L, Milić-Komić S, Zivanović B, Veljović-Jovanović S. Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology. 2016;206:25-39.
doi:10.1016/j.jplph.2016.07.017 .

Vidović, Marija, Morina, Filis, Prokić, Ljiljana, Milić-Komić, Sonja, Zivanović, Bojana, Veljović-Jovanović, Sonja, "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat" in Journal of Plant Physiology, 206 (2016):25-39,
https://doi.org/10.1016/j.jplph.2016.07.017 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić-Komić, Sonja
AU  - Vuleta, A.
AU  - Zechmann, B.
AU  - Prokić, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4032
AB  - Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate-glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.
PB  - Wiley, Hoboken
T2  - Plant Biology
T1  - Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants
EP  - 680
IS  - 4
SP  - 669
VL  - 18
DO  - 10.1111/plb.12429
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić-Komić, Sonja and Vuleta, A. and Zechmann, B. and Prokić, Ljiljana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate-glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.",
publisher = "Wiley, Hoboken",
journal = "Plant Biology",
title = "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants",
pages = "680-669",
number = "4",
volume = "18",
doi = "10.1111/plb.12429"
}

Vidović, M., Morina, F., Milić-Komić, S., Vuleta, A., Zechmann, B., Prokić, L.,& Veljović-Jovanović, S.. (2016). Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology
Wiley, Hoboken., 18(4), 669-680.
https://doi.org/10.1111/plb.12429

Vidović M, Morina F, Milić-Komić S, Vuleta A, Zechmann B, Prokić L, Veljović-Jovanović S. Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology. 2016;18(4):669-680.
doi:10.1111/plb.12429 .

Vidović, Marija, Morina, Filis, Milić-Komić, Sonja, Vuleta, A., Zechmann, B., Prokić, Ljiljana, Veljović-Jovanović, Sonja, "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants" in Plant Biology, 18, no. 4 (2016):669-680,
https://doi.org/10.1111/plb.12429 . .