TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Poleksić, Vesna
AU  - Rašković, Božidar
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5859
AB  - Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Science and Pollution Research
T1  - Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment
DO  - 10.1007/s11356-021-14526-w
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Poleksić, Vesna and Rašković, Božidar",
year = "2021",
abstract = "Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Science and Pollution Research",
title = "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment",
doi = "10.1007/s11356-021-14526-w"
}

Nikolić, D., Skorić, S., Poleksić, V.,& Rašković, B.. (2021). Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s11356-021-14526-w

Nikolić D, Skorić S, Poleksić V, Rašković B. Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research. 2021;.
doi:10.1007/s11356-021-14526-w .

Nikolić, Dušan, Skorić, Stefan, Poleksić, Vesna, Rašković, Božidar, "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment" in Environmental Science and Pollution Research (2021),
https://doi.org/10.1007/s11356-021-14526-w . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Rašković, Božidar
AU  - Lenhardt, Mirjana
AU  - Krpo-Cetković, Jasmina
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5326
AB  - This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)
VL  - 244
DO  - 10.1016/j.chemosphere.2019.125503
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Rašković, Božidar and Lenhardt, Mirjana and Krpo-Cetković, Jasmina",
year = "2020",
abstract = "This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)",
volume = "244",
doi = "10.1016/j.chemosphere.2019.125503"
}

Nikolić, D., Skorić, S., Rašković, B., Lenhardt, M.,& Krpo-Cetković, J.. (2020). Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 244.
https://doi.org/10.1016/j.chemosphere.2019.125503

Nikolić D, Skorić S, Rašković B, Lenhardt M, Krpo-Cetković J. Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere. 2020;244.
doi:10.1016/j.chemosphere.2019.125503 .

Nikolić, Dušan, Skorić, Stefan, Rašković, Božidar, Lenhardt, Mirjana, Krpo-Cetković, Jasmina, "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)" in Chemosphere, 244 (2020),
https://doi.org/10.1016/j.chemosphere.2019.125503 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Skorić, Stefan
AU  - Jovicić, Katarina
AU  - Spasić, Sladana
AU  - Hegedis, Aleksandar
AU  - Vasić, Nebojša
AU  - Lenhardt, Mirjana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4745
AB  - Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia
EP  - 247
SP  - 238
VL  - 153
DO  - 10.1016/j.ecoenv.2018.01.058
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Skorić, Stefan and Jovicić, Katarina and Spasić, Sladana and Hegedis, Aleksandar and Vasić, Nebojša and Lenhardt, Mirjana",
year = "2018",
abstract = "Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia",
pages = "247-238",
volume = "153",
doi = "10.1016/j.ecoenv.2018.01.058"
}

Rašković, B., Poleksić, V., Skorić, S., Jovicić, K., Spasić, S., Hegedis, A., Vasić, N.,& Lenhardt, M.. (2018). Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 153, 238-247.
https://doi.org/10.1016/j.ecoenv.2018.01.058

Rašković B, Poleksić V, Skorić S, Jovicić K, Spasić S, Hegedis A, Vasić N, Lenhardt M. Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety. 2018;153:238-247.
doi:10.1016/j.ecoenv.2018.01.058 .

Rašković, Božidar, Poleksić, Vesna, Skorić, Stefan, Jovicić, Katarina, Spasić, Sladana, Hegedis, Aleksandar, Vasić, Nebojša, Lenhardt, Mirjana, "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia" in Ecotoxicology and Environmental Safety, 153 (2018):238-247,
https://doi.org/10.1016/j.ecoenv.2018.01.058 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Visnjić-Jeftić, Zeljka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Djikanović, Vesna
AU  - Jarić, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3690
AB  - The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.
PB  - Wiley, Hoboken
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution
EP  - 1161
IS  - 10
SP  - 1153
VL  - 30
DO  - 10.1002/tox.21988
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Visnjić-Jeftić, Zeljka and Skorić, Stefan and Gačić, Zoran and Djikanović, Vesna and Jarić, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.",
publisher = "Wiley, Hoboken",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution",
pages = "1161-1153",
number = "10",
volume = "30",
doi = "10.1002/tox.21988"
}

Rašković, B., Poleksić, V., Visnjić-Jeftić, Z., Skorić, S., Gačić, Z., Djikanović, V., Jarić, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology
Wiley, Hoboken., 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988

Rašković B, Poleksić V, Visnjić-Jeftić Z, Skorić S, Gačić Z, Djikanović V, Jarić I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .

Rašković, Božidar, Poleksić, Vesna, Visnjić-Jeftić, Zeljka, Skorić, Stefan, Gačić, Zoran, Djikanović, Vesna, Jarić, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .

TY  - JOUR
AU  - Skorić, Stefan
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3021
AB  - Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.
PB  - Springer, Dordrecht
T2  - Aquaculture International
T1  - Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices
EP  - 535
IS  - 3
SP  - 525
VL  - 20
DO  - 10.1007/s10499-011-9483-3
ER  - 

@article{
author = "Skorić, Stefan and Rašković, Božidar and Poleksić, Vesna and Gačić, Zoran and Lenhardt, Mirjana",
year = "2012",
abstract = "Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.",
publisher = "Springer, Dordrecht",
journal = "Aquaculture International",
title = "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices",
pages = "535-525",
number = "3",
volume = "20",
doi = "10.1007/s10499-011-9483-3"
}

Skorić, S., Rašković, B., Poleksić, V., Gačić, Z.,& Lenhardt, M.. (2012). Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International
Springer, Dordrecht., 20(3), 525-535.
https://doi.org/10.1007/s10499-011-9483-3

Skorić S, Rašković B, Poleksić V, Gačić Z, Lenhardt M. Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International. 2012;20(3):525-535.
doi:10.1007/s10499-011-9483-3 .

Skorić, Stefan, Rašković, Božidar, Poleksić, Vesna, Gačić, Zoran, Lenhardt, Mirjana, "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices" in Aquaculture International, 20, no. 3 (2012):525-535,
https://doi.org/10.1007/s10499-011-9483-3 . .