TY  - JOUR
AU  - Jevremović, Darko
AU  - Vasić, Tanja
AU  - Živković, Sanja
AU  - Vasilijević, Bojana
AU  - Marić, Miloš
AU  - Vojvodić, Mira
AU  - Bulajić, Aleksandra
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6082
AB  - Highbush blueberry (Vaccinium corymbosum L.) is becoming an important berry fruit in Serbian agriculture, and the areas under blueberries are rapidly expanding. Since 2011, blueberry bushes with the symptoms of twig dieback and shoot canker were noticed in four important producing regions in the country. To identify the causal agent, 40 symptomatic bushes from four districts were sampled and analyzed. Based on morphological characteristics, all analyzed isolates were preliminary identified as Neopestalotiopsis clavispora. Selected isolates were further characterized by sequence analysis of the rDNA internal transcribed spacer region, translation elongation factor 1-alpha, and β-tubulin (TUB). Sequence and phylogenetic analysis confirmed that all isolates belong to N. clavispora. The pathogenicity tests on blueberry ‘Elliot’ confirmed that tested isolates were pathogenic. The detection of N. clavispora, a causal agent of twig dieback on blueberries in Serbia, is the first report of this pathogen in the country.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Journal of Plant Diseases and Protection
T1  - Neopestalotiopsis clavispora: a causal agent of twig dieback on highbush blueberries in Serbia
DO  - 10.1007/s41348-022-00610-x
ER  - 

@article{
author = "Jevremović, Darko and Vasić, Tanja and Živković, Sanja and Vasilijević, Bojana and Marić, Miloš and Vojvodić, Mira and Bulajić, Aleksandra",
year = "2022",
abstract = "Highbush blueberry (Vaccinium corymbosum L.) is becoming an important berry fruit in Serbian agriculture, and the areas under blueberries are rapidly expanding. Since 2011, blueberry bushes with the symptoms of twig dieback and shoot canker were noticed in four important producing regions in the country. To identify the causal agent, 40 symptomatic bushes from four districts were sampled and analyzed. Based on morphological characteristics, all analyzed isolates were preliminary identified as Neopestalotiopsis clavispora. Selected isolates were further characterized by sequence analysis of the rDNA internal transcribed spacer region, translation elongation factor 1-alpha, and β-tubulin (TUB). Sequence and phylogenetic analysis confirmed that all isolates belong to N. clavispora. The pathogenicity tests on blueberry ‘Elliot’ confirmed that tested isolates were pathogenic. The detection of N. clavispora, a causal agent of twig dieback on blueberries in Serbia, is the first report of this pathogen in the country.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Journal of Plant Diseases and Protection",
title = "Neopestalotiopsis clavispora: a causal agent of twig dieback on highbush blueberries in Serbia",
doi = "10.1007/s41348-022-00610-x"
}

Jevremović, D., Vasić, T., Živković, S., Vasilijević, B., Marić, M., Vojvodić, M.,& Bulajić, A.. (2022). Neopestalotiopsis clavispora: a causal agent of twig dieback on highbush blueberries in Serbia. in Journal of Plant Diseases and Protection
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s41348-022-00610-x

Jevremović D, Vasić T, Živković S, Vasilijević B, Marić M, Vojvodić M, Bulajić A. Neopestalotiopsis clavispora: a causal agent of twig dieback on highbush blueberries in Serbia. in Journal of Plant Diseases and Protection. 2022;.
doi:10.1007/s41348-022-00610-x .

Jevremović, Darko, Vasić, Tanja, Živković, Sanja, Vasilijević, Bojana, Marić, Miloš, Vojvodić, Mira, Bulajić, Aleksandra, "Neopestalotiopsis clavispora: a causal agent of twig dieback on highbush blueberries in Serbia" in Journal of Plant Diseases and Protection (2022),
https://doi.org/10.1007/s41348-022-00610-x . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5227
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Stevanović, Milos
AU  - Ristić, Danijela
AU  - Zivković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5044
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Milos and Ristić, Danijela and Zivković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Zivković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Zivković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Milos, Ristić, Danijela, Zivković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Vojvodić, Mira
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5213
AB  - Fungal diseases of cucurbits are caused by various fungi and fungus-like organisms which are frequent limiting factor in the production of cucurbit crops all over the world. Mycoses of cucurbits are usually categorized into three overlapping groups, disease of subterranean parts, diseases of aerial parts and fruit rots. Although each group comprises numerous, nonrelated fungal species with different taxonomic position, they share several epidemiological features demanding similar control strategies which can be successfully applied. Diseases of subterranean organs of cucurbits are caused by several soil-borne fungi and fungus-like organisms. The most frequent diseases of this group are seed rot and seedling root rot (caused by Pythium spp.), seedling damping-off and root and crown rot (Phytophthora spp., Rhizoctonia spp., Sclerotinia sclerotiorum), fusarium wilt (Fusarium spp.), verticilium wilt (Verticillium albo-atrum i V. dahliae) and other. The most common diseases of aerial parts, also known as diseases of cucurbits leaves are alternaria leaf spot and leaf blight (caused by Alternaria cucumerina, A. alternata), gummy stem blight (Didymella bryoniae), anthracnose (Colletotrichum orbiculare), downy mildew (Pseudoperonospora cubensis), powdery mildew (Podosphaera xanthii and Golovinomyces cichoracearum), cercospora leaf spot (Cercospora citrullina) and others. Diseases of cucurbit fruits are economically very important and are caused either as fi nal stage of several diseases of leaves or by fruit infection and colonization by soil-borne fungi. The most common causing agents worldwide are Phytophthora capsici, several Fusarium spp., Alternaria alternata, Rhizoctonia solani, Didymella bryoniae, Sclerotium rolfsii and others. Managing strategies for successful control of cucurbit diseases are based to target disease prevention, on one hand, and to slow down disease spreading. Starting the production with pathogenfree seed is crucial fi rst step in disease prevention coupled with growing disease resistant cultivars and using wide crop rotation schedule. The disease spread can be slowed by direct application of chemical or biological fungicide when necessary.
AB  - Mikoze vrežastih kultura mogu biti izazvane raznorodnim vrstama gljiva i pseudogljiva i čest su ograničavajući faktor u proizvodnji u mnogim delovima sveta. Uobičajeno je da se svrstavaju u tri osnovne grupe, bolesti podzemnih organa, bolesti nadzemnih organa i bolesti ploda pre i posle berbe. U svakoj od ovih grupa gljive i pseudogljive prouzrokovači su brojni i taksonomski veoma udaljeni, ali među njima postoje izvesne sličnosti u epidemiologiji zbog čega se za suzbijanje mogu primeniti neke relativno slične strategije. Više vrsta gljiva i pseudogljiva koje se održavaju u zemljištu (eng. soilborne fungi) obično su prouzrokovači bolesti podzemnih organa vrežastih kultura. Uobičajene bolesti podzemnih organa su: rano propadanje tokom klijanja i nicanja (prouzrokovači vrste roda Pythium spp.), propadanje ili topljenje sejanaca (Phytophthora spp., Rhizoctonia spp., Sclerotinia sclerotiorum), fuzariozno uvenuće i sušenje biljaka (Fusarium spp.), verticiliozna uvelost (Verticillium alboatrum i V. dahliae) i druge. Bolesti lista vrežastih kultura javljaju se ili isključivo na listovima ili tu započinju razvoj, a potom napadaju i vrežu i ostale biljne organe. Među značajnije bolesti lista vrežastih kultura ubrajaju se alternarijska pegavost lista (prouzrokovači Alternaria cucumerina, A. alternata), gumozno sušenje stabla (Didymella bryoniae), antraknoza (Colletotrichum orbiculare), plamenjača (Pseudoperonospora cubensis), pepelnica (Podosphaera xanthii i Golovinomyces cichoracearum), pegavost lista (Cercospora citrullina) i druge. Bolesti ploda vrežastih kultura su ekonomski veoma značajne i nastaju usled zaraze listova i stabla ili gljiva i pseudogljiva poreklom iz zemljišta sa kojima plod može biti u direktnom kontaktu. Najčešći prouzrokovači su Phytophthora capsici, vekoliko vrsta Fusarium spp., Alternaria alternata, Rhizoctonia solani, Didymella bryoniae, Sclerotium rolfsii i druge. Efi kasna kontrola bolesti vrežastih kultura zasniva se pre svega na preventivnim i agrotehničkim merama koje sprečavaju nastanak ili usporavaju širenje bolesti. Setva zdravog semena i gajenje otpornih genotipova i plodored, svakako su najznačajnije, kao i direktna primena hemijskih ili bioloških fungicida.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Fungal diseases of cucurbits
T1  - Gljive i pseudogljive prouzrokovači bolesti vrežastih kultura
EP  - 430
IS  - 6
SP  - 418
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5213
ER  - 

@article{
author = "Bulajić, Aleksandra and Vojvodić, Mira",
year = "2019",
abstract = "Fungal diseases of cucurbits are caused by various fungi and fungus-like organisms which are frequent limiting factor in the production of cucurbit crops all over the world. Mycoses of cucurbits are usually categorized into three overlapping groups, disease of subterranean parts, diseases of aerial parts and fruit rots. Although each group comprises numerous, nonrelated fungal species with different taxonomic position, they share several epidemiological features demanding similar control strategies which can be successfully applied. Diseases of subterranean organs of cucurbits are caused by several soil-borne fungi and fungus-like organisms. The most frequent diseases of this group are seed rot and seedling root rot (caused by Pythium spp.), seedling damping-off and root and crown rot (Phytophthora spp., Rhizoctonia spp., Sclerotinia sclerotiorum), fusarium wilt (Fusarium spp.), verticilium wilt (Verticillium albo-atrum i V. dahliae) and other. The most common diseases of aerial parts, also known as diseases of cucurbits leaves are alternaria leaf spot and leaf blight (caused by Alternaria cucumerina, A. alternata), gummy stem blight (Didymella bryoniae), anthracnose (Colletotrichum orbiculare), downy mildew (Pseudoperonospora cubensis), powdery mildew (Podosphaera xanthii and Golovinomyces cichoracearum), cercospora leaf spot (Cercospora citrullina) and others. Diseases of cucurbit fruits are economically very important and are caused either as fi nal stage of several diseases of leaves or by fruit infection and colonization by soil-borne fungi. The most common causing agents worldwide are Phytophthora capsici, several Fusarium spp., Alternaria alternata, Rhizoctonia solani, Didymella bryoniae, Sclerotium rolfsii and others. Managing strategies for successful control of cucurbit diseases are based to target disease prevention, on one hand, and to slow down disease spreading. Starting the production with pathogenfree seed is crucial fi rst step in disease prevention coupled with growing disease resistant cultivars and using wide crop rotation schedule. The disease spread can be slowed by direct application of chemical or biological fungicide when necessary., Mikoze vrežastih kultura mogu biti izazvane raznorodnim vrstama gljiva i pseudogljiva i čest su ograničavajući faktor u proizvodnji u mnogim delovima sveta. Uobičajeno je da se svrstavaju u tri osnovne grupe, bolesti podzemnih organa, bolesti nadzemnih organa i bolesti ploda pre i posle berbe. U svakoj od ovih grupa gljive i pseudogljive prouzrokovači su brojni i taksonomski veoma udaljeni, ali među njima postoje izvesne sličnosti u epidemiologiji zbog čega se za suzbijanje mogu primeniti neke relativno slične strategije. Više vrsta gljiva i pseudogljiva koje se održavaju u zemljištu (eng. soilborne fungi) obično su prouzrokovači bolesti podzemnih organa vrežastih kultura. Uobičajene bolesti podzemnih organa su: rano propadanje tokom klijanja i nicanja (prouzrokovači vrste roda Pythium spp.), propadanje ili topljenje sejanaca (Phytophthora spp., Rhizoctonia spp., Sclerotinia sclerotiorum), fuzariozno uvenuće i sušenje biljaka (Fusarium spp.), verticiliozna uvelost (Verticillium alboatrum i V. dahliae) i druge. Bolesti lista vrežastih kultura javljaju se ili isključivo na listovima ili tu započinju razvoj, a potom napadaju i vrežu i ostale biljne organe. Među značajnije bolesti lista vrežastih kultura ubrajaju se alternarijska pegavost lista (prouzrokovači Alternaria cucumerina, A. alternata), gumozno sušenje stabla (Didymella bryoniae), antraknoza (Colletotrichum orbiculare), plamenjača (Pseudoperonospora cubensis), pepelnica (Podosphaera xanthii i Golovinomyces cichoracearum), pegavost lista (Cercospora citrullina) i druge. Bolesti ploda vrežastih kultura su ekonomski veoma značajne i nastaju usled zaraze listova i stabla ili gljiva i pseudogljiva poreklom iz zemljišta sa kojima plod može biti u direktnom kontaktu. Najčešći prouzrokovači su Phytophthora capsici, vekoliko vrsta Fusarium spp., Alternaria alternata, Rhizoctonia solani, Didymella bryoniae, Sclerotium rolfsii i druge. Efi kasna kontrola bolesti vrežastih kultura zasniva se pre svega na preventivnim i agrotehničkim merama koje sprečavaju nastanak ili usporavaju širenje bolesti. Setva zdravog semena i gajenje otpornih genotipova i plodored, svakako su najznačajnije, kao i direktna primena hemijskih ili bioloških fungicida.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Fungal diseases of cucurbits, Gljive i pseudogljive prouzrokovači bolesti vrežastih kultura",
pages = "430-418",
number = "6",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5213"
}

Bulajić, A.,& Vojvodić, M.. (2019). Fungal diseases of cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(6), 418-430.
https://hdl.handle.net/21.15107/rcub_agrospace_5213

Bulajić A, Vojvodić M. Fungal diseases of cucurbits. in Biljni lekar. 2019;47(6):418-430.
https://hdl.handle.net/21.15107/rcub_agrospace_5213 .

Bulajić, Aleksandra, Vojvodić, Mira, "Fungal diseases of cucurbits" in Biljni lekar, 47, no. 6 (2019):418-430,
https://hdl.handle.net/21.15107/rcub_agrospace_5213 .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4898
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Radović, Nikola
AU  - Zečević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4649
AB  - In a two-year survey (2011-2012), 3220 samples were collected and analyzed in order to determine the presence and distribution of viruses in tomato crops at 56 localities of 18 districts in Serbia. Out of 12 viruses tested, Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) were detected in 42.1, 40, 11, 8.6, 2.3 and 1.3% of the total tested samples, respectively. The results revealed that CMV was prevalent in 2011 and PVY in 2012. CMV and PVY, apart from being predominant, were also the most widespread viruses. In general, single infections were the most frequent type of infection. Additionally, the most common mixed infections were double infections and the most prevalent combination was CMV and PVY. In 2011, the incidence of diseases and the percentage of all infection types were significantly higher than in 2012. Furthermore, in 2011, regardless of total single infections being prevalent compared to mixed infections, two prevailing viruses were commonly detected in mixed infections. The additional molecular testing of ELISA-negative samples using virus specific primers did not reveal the presence of Pepino mosaic virus (PepMV), Tomato yellow leaf curl virus (TYLC), Tomato infections chlorosis virus (TICV) and Tomato chlorosis virus (ToCV).
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Viruses affecting tomato crops in Serbia
EP  - 235
IS  - 1
SP  - 225
VL  - 152
DO  - 10.1007/s10658-018-1467-y
ER  - 

@article{
author = "Nikolić, Dušan and Vučurović, Ana and Stanković, Ivana and Radović, Nikola and Zečević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2018",
abstract = "In a two-year survey (2011-2012), 3220 samples were collected and analyzed in order to determine the presence and distribution of viruses in tomato crops at 56 localities of 18 districts in Serbia. Out of 12 viruses tested, Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) were detected in 42.1, 40, 11, 8.6, 2.3 and 1.3% of the total tested samples, respectively. The results revealed that CMV was prevalent in 2011 and PVY in 2012. CMV and PVY, apart from being predominant, were also the most widespread viruses. In general, single infections were the most frequent type of infection. Additionally, the most common mixed infections were double infections and the most prevalent combination was CMV and PVY. In 2011, the incidence of diseases and the percentage of all infection types were significantly higher than in 2012. Furthermore, in 2011, regardless of total single infections being prevalent compared to mixed infections, two prevailing viruses were commonly detected in mixed infections. The additional molecular testing of ELISA-negative samples using virus specific primers did not reveal the presence of Pepino mosaic virus (PepMV), Tomato yellow leaf curl virus (TYLC), Tomato infections chlorosis virus (TICV) and Tomato chlorosis virus (ToCV).",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Viruses affecting tomato crops in Serbia",
pages = "235-225",
number = "1",
volume = "152",
doi = "10.1007/s10658-018-1467-y"
}

Nikolić, D., Vučurović, A., Stanković, I., Radović, N., Zečević, K., Bulajić, A.,& Krstić, B.. (2018). Viruses affecting tomato crops in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 152(1), 225-235.
https://doi.org/10.1007/s10658-018-1467-y

Nikolić D, Vučurović A, Stanković I, Radović N, Zečević K, Bulajić A, Krstić B. Viruses affecting tomato crops in Serbia. in European Journal of Plant Pathology. 2018;152(1):225-235.
doi:10.1007/s10658-018-1467-y .

Nikolić, Dušan, Vučurović, Ana, Stanković, Ivana, Radović, Nikola, Zečević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "Viruses affecting tomato crops in Serbia" in European Journal of Plant Pathology, 152, no. 1 (2018):225-235,
https://doi.org/10.1007/s10658-018-1467-y . .

TY  - JOUR
AU  - Živković, Sanja
AU  - Bulajić, Aleksandra
AU  - Vasić, Tanja
AU  - Ivanović, Milan
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4840
AB  - The eutypa dieback of grapevine (Vitis vinifera L.), caused by phytopathogenic fungus Eutypa lata, is one of the most severe diseases of trunk and other woody tissues of grapevine in the world. This disease, also known as the grapevine eutyposis, significantly reduces yield and quality of grapes, but primarily reduces the life span of the vines, causing partial or complete dying of grapevine. During 2004-2012, symptoms of dieback of grapevine were observed in Serbia and E. lata presence was confirmed by conventional and molecular identification methods. Symptoms first appear in the form of tiny, chlorotic and necrotic spots on the periphery of the leaf, followed by deformation of the leaves and the appearance of shortened shoots, often with the so-called zigzag growth of internodes. Subsequently, partial or complete dying of the grapevine occurs. The causal agent of eutypa dieback is E. lata - a vascular pathogen from the Diatrypaceae family. The infection occurs when ascospores reach fresh wounds formed by pruning, and in the presence of water droplets penetrate into the vascular tissue. Wounds are particularly sensitive immediately after pruning, although infections can occur seven weeks after the pruning. Eutyposis disease control requires integration of several different control measures. When pruning the grapevine, large scale wounding should be avoided. Pruned parts from diseased plants should be taken out of the vineyard and burned. If the symptoms are present, but the disease has not spread to the whole vine, the infected tissue should be pruned off together with at least 10 cm of healthy tissue. Fresh cuts should be preferably coated with fungicides such as thiophanate-methyl, pyraclostrobin or tebuconazole. In USA, paste with 5% (w/w) boric acid is successfully used for the coating of wounds. It is especially important to protect cuts during the restoration of grapevines or grafting grapevine on the two-year-old and the older grapevine, as well as in the large cuts inflicted in the main trunk. In the USA (California), it is recommended to conduct pruning as late as possible, just before bud swelling. Preventive measures include selection of less sensitive varieties and removal of potential disease hosts near the vineyard. Breeding forms of grapevine with lower stems and mixed pruning are also recommended.
AB  - Eutipozno odumiranje čokota vinove loze (Vitis vinifera L.), kojе prouzokuje fitopatogena gljiva Eutypa lata, predstavlja jednu od najštetnijih bolesti debla i ostalih drvenastih tkiva vinove loze u svetu. Ovо oboljenje, poznato i kao eutipoza vinove loze, značajno smanjuje prinos i kvalitet grožđa, ali pre svega skraćuje životni vek vinove loze, izazivajući delimično ili potpuno odumiranje čokota. U peroiodu od 2004 do 2012. godine, na teritoriji Srbije, uočeni su simptomi odumiranja čokota vinove loze, a prisustvo E. lata potvrđeno primenom konvencionalnih i molekularnih metoda. Simptomi se najpre ispoljavaju u vidu sitnih, hlorotičnih i nekrotičnih pega po obodu lista, praćeni deformacijom listova i pojavom skraćenih lastara, često sa takozvanim cik-cak porastom internodija. Vremenom dolazi do delimičnog ili potpunog odumiranja čokota vinove loze. Prouzrokovač eutipoznog odumiranja vinove loze je gljiva E. lata - vaskularni patogen iz familije Diatrypaceae. Infekcija nastaje kada askospore gljive dospeju na sveže preseke nastale rezidbom i u prisustvu vodenih kapi prodiru u vaskularno tkivo. Rane su posebno osetljive odmah nakon orezivanja, premda se infekcije mogu ostvariti i sedam nedelja nakon rezidbe. Mere zaštite od ove kompleksne bolesti zahtevaju integraciju više različitih mera borbe. Pri orezivanju vinove loze potrebno je izbegavati nanošenje velikih rana. Ukoliko se primete simptomi, ali se bolest nije proširila na ceo čokot, zaraženo tkivo treba odrezati zajedno sa najmanje 10 cm zdravog tkiva. Orezane delove sa obolelih biljaka bi trebalo izneti iz vinograda i spaliti. Sveže preseke poželjno je premazati fungicidima kao što su tiofanat - metil, piraklostrobin ili tebukonazol. Za premazivanje rana u SAD-a se uspešno koristi pasta sa 5% (w/w) bornom kiselinom. Posebno je važno zaštititi preseke pri obnavljaju čokota ili prekalemljivanju loze na dvogodišnjoj i starijoj lozi, kao i velike preseke na glavnom stablu. U SAD (Kaliforniji) preporučuje se što kasnija rezidba, ako je moguće pred samo bubrenje pupoljaka. Preventivne mere uključuju izbor manje osetljivih sorti i uklanjanje potencijalnih domaćina prouzrokovača u blizini vinograda. Za preventivu se preporučuje i izbor nekog od uzgojnih oblika koji podrazumevaju formiranje čokota sa niskim stablom - mešovita rezidba.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Eutypa dieback of grapevine
T1  - Eutipozno odumiranje čokota vinove loze
EP  - 658
IS  - 6
SP  - 647
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4840
ER  - 

@article{
author = "Živković, Sanja and Bulajić, Aleksandra and Vasić, Tanja and Ivanović, Milan",
year = "2018",
abstract = "The eutypa dieback of grapevine (Vitis vinifera L.), caused by phytopathogenic fungus Eutypa lata, is one of the most severe diseases of trunk and other woody tissues of grapevine in the world. This disease, also known as the grapevine eutyposis, significantly reduces yield and quality of grapes, but primarily reduces the life span of the vines, causing partial or complete dying of grapevine. During 2004-2012, symptoms of dieback of grapevine were observed in Serbia and E. lata presence was confirmed by conventional and molecular identification methods. Symptoms first appear in the form of tiny, chlorotic and necrotic spots on the periphery of the leaf, followed by deformation of the leaves and the appearance of shortened shoots, often with the so-called zigzag growth of internodes. Subsequently, partial or complete dying of the grapevine occurs. The causal agent of eutypa dieback is E. lata - a vascular pathogen from the Diatrypaceae family. The infection occurs when ascospores reach fresh wounds formed by pruning, and in the presence of water droplets penetrate into the vascular tissue. Wounds are particularly sensitive immediately after pruning, although infections can occur seven weeks after the pruning. Eutyposis disease control requires integration of several different control measures. When pruning the grapevine, large scale wounding should be avoided. Pruned parts from diseased plants should be taken out of the vineyard and burned. If the symptoms are present, but the disease has not spread to the whole vine, the infected tissue should be pruned off together with at least 10 cm of healthy tissue. Fresh cuts should be preferably coated with fungicides such as thiophanate-methyl, pyraclostrobin or tebuconazole. In USA, paste with 5% (w/w) boric acid is successfully used for the coating of wounds. It is especially important to protect cuts during the restoration of grapevines or grafting grapevine on the two-year-old and the older grapevine, as well as in the large cuts inflicted in the main trunk. In the USA (California), it is recommended to conduct pruning as late as possible, just before bud swelling. Preventive measures include selection of less sensitive varieties and removal of potential disease hosts near the vineyard. Breeding forms of grapevine with lower stems and mixed pruning are also recommended., Eutipozno odumiranje čokota vinove loze (Vitis vinifera L.), kojе prouzokuje fitopatogena gljiva Eutypa lata, predstavlja jednu od najštetnijih bolesti debla i ostalih drvenastih tkiva vinove loze u svetu. Ovо oboljenje, poznato i kao eutipoza vinove loze, značajno smanjuje prinos i kvalitet grožđa, ali pre svega skraćuje životni vek vinove loze, izazivajući delimično ili potpuno odumiranje čokota. U peroiodu od 2004 do 2012. godine, na teritoriji Srbije, uočeni su simptomi odumiranja čokota vinove loze, a prisustvo E. lata potvrđeno primenom konvencionalnih i molekularnih metoda. Simptomi se najpre ispoljavaju u vidu sitnih, hlorotičnih i nekrotičnih pega po obodu lista, praćeni deformacijom listova i pojavom skraćenih lastara, često sa takozvanim cik-cak porastom internodija. Vremenom dolazi do delimičnog ili potpunog odumiranja čokota vinove loze. Prouzrokovač eutipoznog odumiranja vinove loze je gljiva E. lata - vaskularni patogen iz familije Diatrypaceae. Infekcija nastaje kada askospore gljive dospeju na sveže preseke nastale rezidbom i u prisustvu vodenih kapi prodiru u vaskularno tkivo. Rane su posebno osetljive odmah nakon orezivanja, premda se infekcije mogu ostvariti i sedam nedelja nakon rezidbe. Mere zaštite od ove kompleksne bolesti zahtevaju integraciju više različitih mera borbe. Pri orezivanju vinove loze potrebno je izbegavati nanošenje velikih rana. Ukoliko se primete simptomi, ali se bolest nije proširila na ceo čokot, zaraženo tkivo treba odrezati zajedno sa najmanje 10 cm zdravog tkiva. Orezane delove sa obolelih biljaka bi trebalo izneti iz vinograda i spaliti. Sveže preseke poželjno je premazati fungicidima kao što su tiofanat - metil, piraklostrobin ili tebukonazol. Za premazivanje rana u SAD-a se uspešno koristi pasta sa 5% (w/w) bornom kiselinom. Posebno je važno zaštititi preseke pri obnavljaju čokota ili prekalemljivanju loze na dvogodišnjoj i starijoj lozi, kao i velike preseke na glavnom stablu. U SAD (Kaliforniji) preporučuje se što kasnija rezidba, ako je moguće pred samo bubrenje pupoljaka. Preventivne mere uključuju izbor manje osetljivih sorti i uklanjanje potencijalnih domaćina prouzrokovača u blizini vinograda. Za preventivu se preporučuje i izbor nekog od uzgojnih oblika koji podrazumevaju formiranje čokota sa niskim stablom - mešovita rezidba.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Eutypa dieback of grapevine, Eutipozno odumiranje čokota vinove loze",
pages = "658-647",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4840"
}

Živković, S., Bulajić, A., Vasić, T.,& Ivanović, M.. (2018). Eutypa dieback of grapevine. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 647-658.
https://hdl.handle.net/21.15107/rcub_agrospace_4840

Živković S, Bulajić A, Vasić T, Ivanović M. Eutypa dieback of grapevine. in Biljni lekar. 2018;46(6):647-658.
https://hdl.handle.net/21.15107/rcub_agrospace_4840 .

Živković, Sanja, Bulajić, Aleksandra, Vasić, Tanja, Ivanović, Milan, "Eutypa dieback of grapevine" in Biljni lekar, 46, no. 6 (2018):647-658,
https://hdl.handle.net/21.15107/rcub_agrospace_4840 .

TY  - CONF
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Milojević, Katarina
AU  - Krstić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4428
AB  - After its first detection in 2005, Alternaria petroselini, the causal agent of black leaf spot, has become one of the most important pathogens of parsley (Petroselinum crispum (Mill.) Nym.) in Serbia. During almost a decade, isolates of A. petroselini originating from parsley leaves, seed and infested soil were collected and identified on the bases of morphological, pathogenic and molecular features. All morphological features were consistent with those described previously, including dark brown colonies on potato dextrose agar (PDA) and subsurface microsclerotia production, and the presence of pigmented, broadly ellipsoidal, singly produced feodyctiosporic conidia. Pathogenicity and host range studies using spray inoculation of spore suspensions, revealed that isolates were pathogenic for parsley, parsnip, celery and coriander, weakly pathogenic for carrot, fennel and anise, and not pathogenic for onion, cabbage, pepper, tomato, and cucumber. Molecular detection utilizing polymerase chain reaction (PCR) and A. radicina specific primers failed to demonstrate a difference between A. petroselini and A. radicina, amplifying isolates belonging to both species. For further confirmation of Serbian A. petroselini isolates, sequencing of selected genomic fragments including ITS and mitochondrial small subunit (mtSSU) rDNA and major allergen Alt a1, b-tubulin, and translation elongation factor-1 alpha (EF1a) protein coding genes was performed. All obtained sequences were deposited in GenBank and BLAST search for the closest matching revealed several A. petroselini isolates from GenBank to share 99-100% nucleotide identity. Parsley is traditionally grown herb and vegetable plant in Serbia and A. petroselini causes considerable damage every year and occasionally very severe yield losses, resulting in significant economic impact, especially in parsley leaf production. Further investigation of the epidemiology and population structure of A. petroselini is needed in order to establish efficient control measures.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - International Symposium on Carrot and other Apiaceae
T1  - Alternaria petroselini pathogen of parsley in Serbia
EP  - 244
SP  - 237
VL  - 1153
DO  - 10.17660/ActaHortic.2017.1153.35
ER  - 

@conference{
author = "Bulajić, Aleksandra and Stanković, Ivana and Milojević, Katarina and Krstić, Branka",
year = "2017",
abstract = "After its first detection in 2005, Alternaria petroselini, the causal agent of black leaf spot, has become one of the most important pathogens of parsley (Petroselinum crispum (Mill.) Nym.) in Serbia. During almost a decade, isolates of A. petroselini originating from parsley leaves, seed and infested soil were collected and identified on the bases of morphological, pathogenic and molecular features. All morphological features were consistent with those described previously, including dark brown colonies on potato dextrose agar (PDA) and subsurface microsclerotia production, and the presence of pigmented, broadly ellipsoidal, singly produced feodyctiosporic conidia. Pathogenicity and host range studies using spray inoculation of spore suspensions, revealed that isolates were pathogenic for parsley, parsnip, celery and coriander, weakly pathogenic for carrot, fennel and anise, and not pathogenic for onion, cabbage, pepper, tomato, and cucumber. Molecular detection utilizing polymerase chain reaction (PCR) and A. radicina specific primers failed to demonstrate a difference between A. petroselini and A. radicina, amplifying isolates belonging to both species. For further confirmation of Serbian A. petroselini isolates, sequencing of selected genomic fragments including ITS and mitochondrial small subunit (mtSSU) rDNA and major allergen Alt a1, b-tubulin, and translation elongation factor-1 alpha (EF1a) protein coding genes was performed. All obtained sequences were deposited in GenBank and BLAST search for the closest matching revealed several A. petroselini isolates from GenBank to share 99-100% nucleotide identity. Parsley is traditionally grown herb and vegetable plant in Serbia and A. petroselini causes considerable damage every year and occasionally very severe yield losses, resulting in significant economic impact, especially in parsley leaf production. Further investigation of the epidemiology and population structure of A. petroselini is needed in order to establish efficient control measures.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "International Symposium on Carrot and other Apiaceae",
title = "Alternaria petroselini pathogen of parsley in Serbia",
pages = "244-237",
volume = "1153",
doi = "10.17660/ActaHortic.2017.1153.35"
}

Bulajić, A., Stanković, I., Milojević, K.,& Krstić, B.. (2017). Alternaria petroselini pathogen of parsley in Serbia. in International Symposium on Carrot and other Apiaceae
Int Soc Horticultural Science, Leuven 1., 1153, 237-244.
https://doi.org/10.17660/ActaHortic.2017.1153.35

Bulajić A, Stanković I, Milojević K, Krstić B. Alternaria petroselini pathogen of parsley in Serbia. in International Symposium on Carrot and other Apiaceae. 2017;1153:237-244.
doi:10.17660/ActaHortic.2017.1153.35 .

Bulajić, Aleksandra, Stanković, Ivana, Milojević, Katarina, Krstić, Branka, "Alternaria petroselini pathogen of parsley in Serbia" in International Symposium on Carrot and other Apiaceae, 1153 (2017):237-244,
https://doi.org/10.17660/ActaHortic.2017.1153.35 . .

TY  - CONF
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Nikolić, D.
AU  - Milojević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4434
AB  - Propolis, the mixture of the nectar of flowers collected by honeybees, showed antiviral activity against human and plant viruses. Since Zucchini yellow mosaic virus (ZYMV, Potyvirus, Potyviridae) is the most important pathogen of oilseed pumpkin (Cucurbita pepo var. styriaca) production worldwide, the aim of this study was to evaluate the effect of propolis extract on ZYMV infection in oilseed pumpkin. Propolis extracted by 95% ethanol was diluted in distilled water to concentration of 1, 2.5, 5 and 10%. Effects of different propolis concentration were evaluated in in vitro and in vivo experiments. In vitro, ZYMV inoculum was mixed with different propolis concentrations and oilseed pumpkin plants were inoculated immediately, 1 and 2 hours after mixing. In vivo, oilseed pumpkin plants were sprayed with different propolis concentrations before and after ZYMV inoculation immediately, 1 and 2 h later. In each experiment 10 oilseed pumpkin plants in one true leaf stage were inoculated. Plants inoculated with ZYMV and sprayed with propolis were used as a positive and negative control, respectively. Symptom appearance was checked 10 days post-inoculation. Only 5 and 10% propolis concentration showed effect in reducing symptoms of ZYMV infection, in all experiments. In vitro, number of symptomatic plants was reduced to 80 and 70% after one-hour period, and to 70 and 60% after two-hour period. In vivo, propolis concentrations of 5 and 10% sprayed before inoculation reduced number of symptomatic plants to 70 and 60% after one-hour period, and to 60 and 50% after two-hour period, while concentrations of 5 and 10% sprayed after inoculation, reduced number of symptomatic plants to 80% each after one-hour period, and to 70 and 80% after two-hour period. In this study propolis exhibited a positive effect on reduction of ZYMV infection despite the fact that plants were challenged with higher levels of the virus than it occurs naturally. Encouraging results of propolis capacity for direct virus control should be confirmed in the field under natural conditions.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III International Symposium on Organic Greenhouse Horticulture
T1  - Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin
EP  - 437
SP  - 431
VL  - 1164
DO  - 10.17660/ActaHortic.2017.1164.56
ER  - 

@conference{
author = "Vučurović, Ana and Stanković, Ivana and Nikolić, D. and Milojević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2017",
abstract = "Propolis, the mixture of the nectar of flowers collected by honeybees, showed antiviral activity against human and plant viruses. Since Zucchini yellow mosaic virus (ZYMV, Potyvirus, Potyviridae) is the most important pathogen of oilseed pumpkin (Cucurbita pepo var. styriaca) production worldwide, the aim of this study was to evaluate the effect of propolis extract on ZYMV infection in oilseed pumpkin. Propolis extracted by 95% ethanol was diluted in distilled water to concentration of 1, 2.5, 5 and 10%. Effects of different propolis concentration were evaluated in in vitro and in vivo experiments. In vitro, ZYMV inoculum was mixed with different propolis concentrations and oilseed pumpkin plants were inoculated immediately, 1 and 2 hours after mixing. In vivo, oilseed pumpkin plants were sprayed with different propolis concentrations before and after ZYMV inoculation immediately, 1 and 2 h later. In each experiment 10 oilseed pumpkin plants in one true leaf stage were inoculated. Plants inoculated with ZYMV and sprayed with propolis were used as a positive and negative control, respectively. Symptom appearance was checked 10 days post-inoculation. Only 5 and 10% propolis concentration showed effect in reducing symptoms of ZYMV infection, in all experiments. In vitro, number of symptomatic plants was reduced to 80 and 70% after one-hour period, and to 70 and 60% after two-hour period. In vivo, propolis concentrations of 5 and 10% sprayed before inoculation reduced number of symptomatic plants to 70 and 60% after one-hour period, and to 60 and 50% after two-hour period, while concentrations of 5 and 10% sprayed after inoculation, reduced number of symptomatic plants to 80% each after one-hour period, and to 70 and 80% after two-hour period. In this study propolis exhibited a positive effect on reduction of ZYMV infection despite the fact that plants were challenged with higher levels of the virus than it occurs naturally. Encouraging results of propolis capacity for direct virus control should be confirmed in the field under natural conditions.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III International Symposium on Organic Greenhouse Horticulture",
title = "Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin",
pages = "437-431",
volume = "1164",
doi = "10.17660/ActaHortic.2017.1164.56"
}

Vučurović, A., Stanković, I., Nikolić, D., Milojević, K., Bulajić, A.,& Krstić, B.. (2017). Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin. in III International Symposium on Organic Greenhouse Horticulture
Int Soc Horticultural Science, Leuven 1., 1164, 431-437.
https://doi.org/10.17660/ActaHortic.2017.1164.56

Vučurović A, Stanković I, Nikolić D, Milojević K, Bulajić A, Krstić B. Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin. in III International Symposium on Organic Greenhouse Horticulture. 2017;1164:431-437.
doi:10.17660/ActaHortic.2017.1164.56 .

Vučurović, Ana, Stanković, Ivana, Nikolić, D., Milojević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "Effect of propolis extract on Zucchini yellow mosaic virus inhibition in oilseed pumpkin" in III International Symposium on Organic Greenhouse Horticulture, 1164 (2017):431-437,
https://doi.org/10.17660/ActaHortic.2017.1164.56 . .

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Stevanović, Milan
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Delibašić, Goran
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4584
AB  - Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum.
AB  - Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia
T1  - Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji
EP  - 286
IS  - 3
SP  - 277
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4584
ER  - 

@article{
author = "Obradović, Ana and Stanković, Slavica and Stevanović, Milan and Krnjaja, Vesna and Bulajić, Aleksandra and Delibašić, Goran",
year = "2017",
abstract = "Species of the genus Fusarium are predominant pathogens in cereals worldwide, while F. graminearum is the most distributed among them. This species synthesises a broad spectrum of mycotoxins (fusariotoxins), among which, trichothecene type B (deoxynivalenol-DON) and zearalenone (ZEA) prevail. The isolates from the collection of the Laboratory of Phytopathology of the Maize Research Institute, Zemun Polje were used to study diversity of the F. graminearum species. Twelve selected isolates were collected in the 2005-2016 period from wheat and barley kernels in various locations in Serbia. The aim of this study was to observe variability of the F. graminearum species regarding isolate pathogenicity, mycelium growth, macroconidium size, as well as DON and ZEA production potential by the ELISA method. All the observed isolates indicated pathogenic potential in the field and expressed different viability ranging from 1.75 to 3.75. Besides different viability, microscopic and macroscopic properties of isolates also diverged. The isolates of F. graminearum produced average concentrations of DON amounting 119. 9 and 33.4 ug/g (isolates from wheat and barley, respectively), while the average concentration of ZEA was 40.14 ug/kg (isolates from wheat) and 31.25 ug/kg (isolates from barley). A correlation between production of DON and ZEA was not observed in the isolates of F. graminearum., Vrste roda Fusarium su najčešći patogeni na žitaricama širom sveta, a najzastupljenija među njima je vrsta F. graminearum. Ova vrsta sintetiše širok spektar mikotoksina (fuzariotoksina), među kojima su najzastupljeniji trihoteceni tipa B (deoksinivalenol-DON), a zatim zearalenoni (ZEA). Za proučavanje diverziteta vrste F. graminearum u okviru ovog rada korišćeni su izolati iz kolekcije Laboratorije za fitopatologiju Instituta za kukuruz “Zemun Polje”. Odabrano je 12 izolata koji su prikupljeni u periodu od 2005. do 2016. godine, poreklom sa zrna pšenice i ječma iz različitih lokaliteta na teritoriji Srbije. Cilj rada je bio da se ispita varijabilnost vrste F. graminearum u pogledu patogenosti izolata, porasta micelije, veličine makrokonidija kao i u potencijalu produkcije DON i ZEA pomoću ELISA metode. Svi ispitivani izolati su ispoljili patogenost u polju uz različit stepen agresivnosti koja je varirala od 1,75 do 3,75 (na skali 1-7). Pored varijabilnosti u agresivnosti izolati su bili divergentni i u mikroskopskim i makroskopskim karakteristikama. Izolati F. graminearum su produkovali prosečne koncentracije DON-a 119,9 ug/g (izolati sa pšenice) i 33,4 ug/g (izolati sa ječma), dok je prosečna koncentracija ZEA kod izolata sa pšenice bila 40,14 ug/kg, a kod izolata sa ječma 31,25 ug/kg. Nije uočena korelacija između produkcije DON i ZEA kod izolata F. graminearum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia, Varijabilnost vrste Fusarium graminearum patogena strnih žita u Srbiji",
pages = "286-277",
number = "3",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4584"
}

Obradović, A., Stanković, S., Stevanović, M., Krnjaja, V., Bulajić, A.,& Delibašić, G.. (2017). Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(3), 277-286.
https://hdl.handle.net/21.15107/rcub_agrospace_4584

Obradović A, Stanković S, Stevanović M, Krnjaja V, Bulajić A, Delibašić G. Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia. in Biljni lekar. 2017;45(3):277-286.
https://hdl.handle.net/21.15107/rcub_agrospace_4584 .

Obradović, Ana, Stanković, Slavica, Stevanović, Milan, Krnjaja, Vesna, Bulajić, Aleksandra, Delibašić, Goran, "Variability of the Fusarium graminearum species: Pathogens of small grains in Serbia" in Biljni lekar, 45, no. 3 (2017):277-286,
https://hdl.handle.net/21.15107/rcub_agrospace_4584 .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4166
AB  - In this paper we report on the isolation and identification of three filamentous fungi from grape marc, and antifungal effect of their cell-free culture filtrates on the growth of Botrytis cinerea, causal agent of gray mold. Grape marc is a waste material that has been used as soil amendment in sustainable agriculture. Isolates originating from grape marc were identified on the basis of morphological features and internal transcribed spacer rDNA or beta-tubulin gene sequencing. The presence of three different species, Penicillium paneum, Penicillium chrysogenum and Aspergillus fumigatus has been detected expressing different effect on the growth of B. cinerea. The effect of crude culture filtrates of selected fungi on B. cinerea growth was tested. Heat sensitivity of the established inhibition effect was examined by autoclaving the crude culture filtrate prior to testing. Additional aim was to determine whether antifungal effect was influenced by previous exposure to B. cinerea in dual liquid cultures. Crude culture filtrate of A. fumigatus K16/2 showed the lowest suppression of B. cinerea growth. A maximal percentage inhibition achieved within the study was 38.2%, 39.8% and 23.8 for crude filtrates of P. paneum K7/1, P. chrysogenum K11/1 and A. fumigatus K16/2, respectively. Presence of B. cinerea in dual liquid culture induced significant increase in antifungal capacity of the culture filtrates in comparison to pure culture filtrates of the chosen isolates. The antifungal activity of all of the isolates' culture filtrates retained after heat treatment suggesting the presence of some thermostable antifungal metabolites. The results indicate the complexity and specificity of the interaction between filamentous fungi and B. cinerea. Grape marc is a good source for isolation od B. cinerea fungal antagonists and their antifungal metabolites. Specificity of fungal-fungal interactions suggests that further research on the antagonistic mechanisms and factors affecting them should be studied separately for each pair of antagonists.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea
EP  - 48
IS  - 1
SP  - 37
VL  - 48
DO  - 10.2298/GENSR1601037J
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "In this paper we report on the isolation and identification of three filamentous fungi from grape marc, and antifungal effect of their cell-free culture filtrates on the growth of Botrytis cinerea, causal agent of gray mold. Grape marc is a waste material that has been used as soil amendment in sustainable agriculture. Isolates originating from grape marc were identified on the basis of morphological features and internal transcribed spacer rDNA or beta-tubulin gene sequencing. The presence of three different species, Penicillium paneum, Penicillium chrysogenum and Aspergillus fumigatus has been detected expressing different effect on the growth of B. cinerea. The effect of crude culture filtrates of selected fungi on B. cinerea growth was tested. Heat sensitivity of the established inhibition effect was examined by autoclaving the crude culture filtrate prior to testing. Additional aim was to determine whether antifungal effect was influenced by previous exposure to B. cinerea in dual liquid cultures. Crude culture filtrate of A. fumigatus K16/2 showed the lowest suppression of B. cinerea growth. A maximal percentage inhibition achieved within the study was 38.2%, 39.8% and 23.8 for crude filtrates of P. paneum K7/1, P. chrysogenum K11/1 and A. fumigatus K16/2, respectively. Presence of B. cinerea in dual liquid culture induced significant increase in antifungal capacity of the culture filtrates in comparison to pure culture filtrates of the chosen isolates. The antifungal activity of all of the isolates' culture filtrates retained after heat treatment suggesting the presence of some thermostable antifungal metabolites. The results indicate the complexity and specificity of the interaction between filamentous fungi and B. cinerea. Grape marc is a good source for isolation od B. cinerea fungal antagonists and their antifungal metabolites. Specificity of fungal-fungal interactions suggests that further research on the antagonistic mechanisms and factors affecting them should be studied separately for each pair of antagonists.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea",
pages = "48-37",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601037J"
}

Jovičić-Petrović, J., Stanković, I., Bulajić, A., Krstić, B., Kiković, D.,& Raičević, V.. (2016). Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 37-48.
https://doi.org/10.2298/GENSR1601037J

Jovičić-Petrović J, Stanković I, Bulajić A, Krstić B, Kiković D, Raičević V. Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea. in Genetika. 2016;48(1):37-48.
doi:10.2298/GENSR1601037J .

Jovičić-Petrović, Jelena, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, Kiković, Dragan, Raičević, Vera, "Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea" in Genetika, 48, no. 1 (2016):37-48,
https://doi.org/10.2298/GENSR1601037J . .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Jeremić, Sanja
AU  - Vucković, Ivan
AU  - Vojnović, Sandra
AU  - Bulajić, Aleksandra
AU  - Raičević, Vera
AU  - Nikodinović-Runić, Jasmina
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4175
AB  - Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum
EP  - 289
IS  - 2
SP  - 279
VL  - 68
DO  - 10.2298/ABS150602016J
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Jeremić, Sanja and Vucković, Ivan and Vojnović, Sandra and Bulajić, Aleksandra and Raičević, Vera and Nikodinović-Runić, Jasmina",
year = "2016",
abstract = "Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum",
pages = "289-279",
number = "2",
volume = "68",
doi = "10.2298/ABS150602016J"
}

Jovičić-Petrović, J., Jeremić, S., Vucković, I., Vojnović, S., Bulajić, A., Raičević, V.,& Nikodinović-Runić, J.. (2016). Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(2), 279-289.
https://doi.org/10.2298/ABS150602016J

Jovičić-Petrović J, Jeremić S, Vucković I, Vojnović S, Bulajić A, Raičević V, Nikodinović-Runić J. Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum. in Archives of Biological Sciences. 2016;68(2):279-289.
doi:10.2298/ABS150602016J .

Jovičić-Petrović, Jelena, Jeremić, Sanja, Vucković, Ivan, Vojnović, Sandra, Bulajić, Aleksandra, Raičević, Vera, Nikodinović-Runić, Jasmina, "Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum" in Archives of Biological Sciences, 68, no. 2 (2016):279-289,
https://doi.org/10.2298/ABS150602016J . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Radović, N.
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Nikolić, D.
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4015
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Infecting Wisteria sinensis in Serbia.
EP  - 1799
IS  - 8
SP  - 1799
VL  - 100
DO  - 10.1094/PDIS-01-16-0096-PDN
ER  - 

@article{
author = "Milojević, Katarina and Radović, N. and Stanković, Ivana and Vučurović, Ana and Nikolić, D. and Bulajić, Aleksandra and Krstić, Branka",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Infecting Wisteria sinensis in Serbia.",
pages = "1799-1799",
number = "8",
volume = "100",
doi = "10.1094/PDIS-01-16-0096-PDN"
}

Milojević, K., Radović, N., Stanković, I., Vučurović, A., Nikolić, D., Bulajić, A.,& Krstić, B.. (2016). First Report of Cucumber mosaic virus Infecting Wisteria sinensis in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(8), 1799-1799.
https://doi.org/10.1094/PDIS-01-16-0096-PDN

Milojević K, Radović N, Stanković I, Vučurović A, Nikolić D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus Infecting Wisteria sinensis in Serbia.. in Plant Disease. 2016;100(8):1799-1799.
doi:10.1094/PDIS-01-16-0096-PDN .

Milojević, Katarina, Radović, N., Stanković, Ivana, Vučurović, Ana, Nikolić, D., Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus Infecting Wisteria sinensis in Serbia." in Plant Disease, 100, no. 8 (2016):1799-1799,
https://doi.org/10.1094/PDIS-01-16-0096-PDN . .

TY  - JOUR
AU  - Vucurović, I.
AU  - Vučurović, Ana
AU  - Nikolić, D.
AU  - Bulajić, Aleksandra
AU  - Milošević, D.
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4149
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Leek yellow stripe virus in Leek in Serbia
EP  - 231
IS  - 1
SP  - 230
VL  - 100
DO  - 10.1094/PDIS-04-15-0432-PDN
ER  - 

@article{
author = "Vucurović, I. and Vučurović, Ana and Nikolić, D. and Bulajić, Aleksandra and Milošević, D. and Krstić, Branka and Stanković, Ivana",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Leek yellow stripe virus in Leek in Serbia",
pages = "231-230",
number = "1",
volume = "100",
doi = "10.1094/PDIS-04-15-0432-PDN"
}

Vucurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B.,& Stanković, I.. (2016). First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 230-231.
https://doi.org/10.1094/PDIS-04-15-0432-PDN

Vucurović I, Vučurović A, Nikolić D, Bulajić A, Milošević D, Krstić B, Stanković I. First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease. 2016;100(1):230-231.
doi:10.1094/PDIS-04-15-0432-PDN .

Vucurović, I., Vučurović, Ana, Nikolić, D., Bulajić, Aleksandra, Milošević, D., Krstić, Branka, Stanković, Ivana, "First Report of Leek yellow stripe virus in Leek in Serbia" in Plant Disease, 100, no. 1 (2016):230-231,
https://doi.org/10.1094/PDIS-04-15-0432-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Marjanović-Jeromela, Ana
AU  - Krstić, Branka
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4234
AB  - A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
AB  - Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia
T1  - Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji
EP  - 44
IS  - 1-2
SP  - 37
VL  - 31
DO  - 10.2298/PIF1602037M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Stanković, Ivana and Bulajić, Aleksandra and Marjanović-Jeromela, Ana and Krstić, Branka",
year = "2016",
abstract = "A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates., Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia, Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji",
pages = "44-37",
number = "1-2",
volume = "31",
doi = "10.2298/PIF1602037M"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Stanković, I., Bulajić, A., Marjanović-Jeromela, A.,& Krstić, B.. (2016). The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 31(1-2), 37-44.
https://doi.org/10.2298/PIF1602037M

Milošević D, Ignjatov M, Nikolić Z, Stanković I, Bulajić A, Marjanović-Jeromela A, Krstić B. The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina. 2016;31(1-2):37-44.
doi:10.2298/PIF1602037M .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Stanković, Ivana, Bulajić, Aleksandra, Marjanović-Jeromela, Ana, Krstić, Branka, "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia" in Pesticidi i fitomedicina, 31, no. 1-2 (2016):37-44,
https://doi.org/10.2298/PIF1602037M . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4269
AB  - Every year, leaf mosaic, yellow streaks, and mottling can be commonly observed in wheat fields worldwide. A number of different factors can cause these symptoms, including nitrogen deficiency, winter injury, and virus diseseas. Today, total number of 44 viruses has been aassociated with the crops and 43 viruses can infect wheat under the experimental conditions. Virus infections in wheat range from latent to lethal. Although not consistently present, diseased fields may yield 5 to 10% less grain. Viruses become economically important when they appear in epidemic proportions. One complex of viruses, Barley yellow dwarf virus (BYDV, Luteovirus, Luteoviridae) is now considered as possibly the most serious disease of wheat and other small grains worldwide. Two other widespread viruses are Wheat streak mosaic virus (WSMV, Tritimovirus, Potyviridae and Soil-borne wheat mosaic virus (SBWMV, Furovirus, Virgaviridae). However, there are additional mite-transmitted viruses, aphidtransmitted viruses, and soilborne viruses that may be important. The emphasis in this article will be on viruses infecting wheat in Serbia: BYDV, WSMV and Brome mosaic virus (BMV, Bromovirus, Bromoviridae). The most practical disease control strategies against wheat viruses are cultivar resistance to the virus and/or to the vector, chemical control of vectors, crop rotation and sanitations.
AB  - Svake godine u usevima pšenice širom sveta uobičajeno je da se uoče simptomi mozaika, žute crticavosti i šarenila lišća. Mnogobrojni faktori mogu da izazovu ovakvu diskoloraciju lišca, uključujući nedostatak azota, kalijuma ili gvožđa, oštećenja od mraza ili virusna oboljenja. Pšenica je prirodni domaćin 44 i eksperimentalni domaćin još 43 različita virusa. Virusi izazivaju na pšenici zaraze različitog intenziteta, od latentnih do letalnih zaraza. Iako nisu stalno prisutni u usevu, zaraze pojedinih godina mogu znatno da smanje prinos, od 5 do 10%. Virusi postaju ekonomski značajni patogeni pšenice kada se jave u epidemijskim razmerama. Najvažnija oboljenja pšenice i drugih žitarica izaziva jedan kompleks virusa, nazvan virus žute patuljavosti ječma (Barley yellow dwarf virus, BYDV, Luteovirus, Luteoviridae). Pored njega, najraširenijim se smatraju virus crticastog mozaika pšenice (Wheat streak mosaic virus, WSMV, Tritimovirus, Potyviridae) i virus mozaika pšenice koji se prenosi zemljišnim pseudogljivama (Soil-borne wheat mosaic virus, SBWMV, Furovirus, Virgaviridae). Pored njih, ekonomski značajnim virusima, smatraju se i drugi virusi koji se prenose grinjama, biljnim vašima ili zemljišnim pseudogljivama. U radu ce biti detaljno opisani virusi koji su prisutni na pšenici u Srbiji: BYDV, WSMV i virus mozaika bromusa (Brome mosaic virus, BMV, Bromovirus, Bromoviridae). Takođe, biće prodiskutovane i najbolje strategije kontrole, kao što su otpornost sorti prema virusima i/ili prema vektorima, hemijska kontrola vektora, plodored i sanitarne mere.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Economically important virus diseases of wheat
T1  - Ekonomski značajne viroze pšenice
EP  - 500
IS  - 5-6
SP  - 486
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4269
ER  - 

@article{
author = "Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra and Vučurović, Ana",
year = "2016",
abstract = "Every year, leaf mosaic, yellow streaks, and mottling can be commonly observed in wheat fields worldwide. A number of different factors can cause these symptoms, including nitrogen deficiency, winter injury, and virus diseseas. Today, total number of 44 viruses has been aassociated with the crops and 43 viruses can infect wheat under the experimental conditions. Virus infections in wheat range from latent to lethal. Although not consistently present, diseased fields may yield 5 to 10% less grain. Viruses become economically important when they appear in epidemic proportions. One complex of viruses, Barley yellow dwarf virus (BYDV, Luteovirus, Luteoviridae) is now considered as possibly the most serious disease of wheat and other small grains worldwide. Two other widespread viruses are Wheat streak mosaic virus (WSMV, Tritimovirus, Potyviridae and Soil-borne wheat mosaic virus (SBWMV, Furovirus, Virgaviridae). However, there are additional mite-transmitted viruses, aphidtransmitted viruses, and soilborne viruses that may be important. The emphasis in this article will be on viruses infecting wheat in Serbia: BYDV, WSMV and Brome mosaic virus (BMV, Bromovirus, Bromoviridae). The most practical disease control strategies against wheat viruses are cultivar resistance to the virus and/or to the vector, chemical control of vectors, crop rotation and sanitations., Svake godine u usevima pšenice širom sveta uobičajeno je da se uoče simptomi mozaika, žute crticavosti i šarenila lišća. Mnogobrojni faktori mogu da izazovu ovakvu diskoloraciju lišca, uključujući nedostatak azota, kalijuma ili gvožđa, oštećenja od mraza ili virusna oboljenja. Pšenica je prirodni domaćin 44 i eksperimentalni domaćin još 43 različita virusa. Virusi izazivaju na pšenici zaraze različitog intenziteta, od latentnih do letalnih zaraza. Iako nisu stalno prisutni u usevu, zaraze pojedinih godina mogu znatno da smanje prinos, od 5 do 10%. Virusi postaju ekonomski značajni patogeni pšenice kada se jave u epidemijskim razmerama. Najvažnija oboljenja pšenice i drugih žitarica izaziva jedan kompleks virusa, nazvan virus žute patuljavosti ječma (Barley yellow dwarf virus, BYDV, Luteovirus, Luteoviridae). Pored njega, najraširenijim se smatraju virus crticastog mozaika pšenice (Wheat streak mosaic virus, WSMV, Tritimovirus, Potyviridae) i virus mozaika pšenice koji se prenosi zemljišnim pseudogljivama (Soil-borne wheat mosaic virus, SBWMV, Furovirus, Virgaviridae). Pored njih, ekonomski značajnim virusima, smatraju se i drugi virusi koji se prenose grinjama, biljnim vašima ili zemljišnim pseudogljivama. U radu ce biti detaljno opisani virusi koji su prisutni na pšenici u Srbiji: BYDV, WSMV i virus mozaika bromusa (Brome mosaic virus, BMV, Bromovirus, Bromoviridae). Takođe, biće prodiskutovane i najbolje strategije kontrole, kao što su otpornost sorti prema virusima i/ili prema vektorima, hemijska kontrola vektora, plodored i sanitarne mere.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Economically important virus diseases of wheat, Ekonomski značajne viroze pšenice",
pages = "500-486",
number = "5-6",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4269"
}

Stanković, I., Krstić, B., Bulajić, A.,& Vučurović, A.. (2016). Economically important virus diseases of wheat. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(5-6), 486-500.
https://hdl.handle.net/21.15107/rcub_agrospace_4269

Stanković I, Krstić B, Bulajić A, Vučurović A. Economically important virus diseases of wheat. in Biljni lekar. 2016;44(5-6):486-500.
https://hdl.handle.net/21.15107/rcub_agrospace_4269 .

Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, Vučurović, Ana, "Economically important virus diseases of wheat" in Biljni lekar, 44, no. 5-6 (2016):486-500,
https://hdl.handle.net/21.15107/rcub_agrospace_4269 .

TY  - JOUR
AU  - Vrandečić, Karolina
AU  - Ćosić, Jasenka
AU  - Novoselović, D.
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3696
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Wheat spindle streak mosaic virus on Wheat in Croatia
EP  - 897
IS  - 6
SP  - 896
VL  - 99
DO  - 10.1094/PDIS-12-14-1288-PDN
ER  - 

@article{
author = "Vrandečić, Karolina and Ćosić, Jasenka and Novoselović, D. and Stanković, Ivana and Vučurović, Ana and Krstić, Branka and Bulajić, Aleksandra",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Wheat spindle streak mosaic virus on Wheat in Croatia",
pages = "897-896",
number = "6",
volume = "99",
doi = "10.1094/PDIS-12-14-1288-PDN"
}

Vrandečić, K., Ćosić, J., Novoselović, D., Stanković, I., Vučurović, A., Krstić, B.,& Bulajić, A.. (2015). First Report of Wheat spindle streak mosaic virus on Wheat in Croatia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(6), 896-897.
https://doi.org/10.1094/PDIS-12-14-1288-PDN

Vrandečić K, Ćosić J, Novoselović D, Stanković I, Vučurović A, Krstić B, Bulajić A. First Report of Wheat spindle streak mosaic virus on Wheat in Croatia. in Plant Disease. 2015;99(6):896-897.
doi:10.1094/PDIS-12-14-1288-PDN .

Vrandečić, Karolina, Ćosić, Jasenka, Novoselović, D., Stanković, Ivana, Vučurović, Ana, Krstić, Branka, Bulajić, Aleksandra, "First Report of Wheat spindle streak mosaic virus on Wheat in Croatia" in Plant Disease, 99, no. 6 (2015):896-897,
https://doi.org/10.1094/PDIS-12-14-1288-PDN . .

TY  - CONF
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Milojević, Katarina
AU  - Nikolić, D.M.
AU  - Teodorović, S.D.
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3673
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Alternaria species on carrot in Serbia
EP  - 21
IS  - 11
SP  - 21
VL  - 105
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3673
ER  - 

@conference{
author = "Bulajić, Aleksandra and Stanković, Ivana and Vučurović, Ana and Milojević, Katarina and Nikolić, D.M. and Teodorović, S.D. and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Alternaria species on carrot in Serbia",
pages = "21-21",
number = "11",
volume = "105",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3673"
}

Bulajić, A., Stanković, I., Vučurović, A., Milojević, K., Nikolić, D.M., Teodorović, S.D.,& Krstić, B.. (2015). Alternaria species on carrot in Serbia. in Phytopathology
Amer Phytopathological Soc, St Paul., 105(11), 21-21.
https://hdl.handle.net/21.15107/rcub_agrospace_3673

Bulajić A, Stanković I, Vučurović A, Milojević K, Nikolić D, Teodorović S, Krstić B. Alternaria species on carrot in Serbia. in Phytopathology. 2015;105(11):21-21.
https://hdl.handle.net/21.15107/rcub_agrospace_3673 .

Bulajić, Aleksandra, Stanković, Ivana, Vučurović, Ana, Milojević, Katarina, Nikolić, D.M., Teodorović, S.D., Krstić, Branka, "Alternaria species on carrot in Serbia" in Phytopathology, 105, no. 11 (2015):21-21,
https://hdl.handle.net/21.15107/rcub_agrospace_3673 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana R.
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3684
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana R. and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}

Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G. R.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M

Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović GR, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .

Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana R., Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Vucurović, I.
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Nikolić, D.
AU  - Teodorović, S.
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3699
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Garlic common latent virus Infecting Garlic in Serbia
EP  - 895
IS  - 6
SP  - 894
VL  - 99
DO  - 10.1094/PDIS-11-14-1229-PDN
ER  - 

@article{
author = "Vučurović, Ana and Vucurović, I. and Stanković, Ivana and Bulajić, Aleksandra and Nikolić, D. and Teodorović, S. and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Garlic common latent virus Infecting Garlic in Serbia",
pages = "895-894",
number = "6",
volume = "99",
doi = "10.1094/PDIS-11-14-1229-PDN"
}

Vučurović, A., Vucurović, I., Stanković, I., Bulajić, A., Nikolić, D., Teodorović, S.,& Krstić, B.. (2015). First Report of Garlic common latent virus Infecting Garlic in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(6), 894-895.
https://doi.org/10.1094/PDIS-11-14-1229-PDN

Vučurović A, Vucurović I, Stanković I, Bulajić A, Nikolić D, Teodorović S, Krstić B. First Report of Garlic common latent virus Infecting Garlic in Serbia. in Plant Disease. 2015;99(6):894-895.
doi:10.1094/PDIS-11-14-1229-PDN .

Vučurović, Ana, Vucurović, I., Stanković, Ivana, Bulajić, Aleksandra, Nikolić, D., Teodorović, S., Krstić, Branka, "First Report of Garlic common latent virus Infecting Garlic in Serbia" in Plant Disease, 99, no. 6 (2015):894-895,
https://doi.org/10.1094/PDIS-11-14-1229-PDN . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Milojević, Katarina
AU  - Vučurović, Ana
AU  - Nikolić, D.
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3714
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium Root Rot of Stored Carrot Caused by Fusarium avenaceum in Serbia.
EP  - 286
IS  - 2
SP  - 286
VL  - 99
DO  - 10.1094/PDIS-07-14-0724-PDN
ER  - 

@article{
author = "Stanković, Ivana and Milojević, Katarina and Vučurović, Ana and Nikolić, D. and Krstić, Branka and Bulajić, Aleksandra",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium Root Rot of Stored Carrot Caused by Fusarium avenaceum in Serbia.",
pages = "286-286",
number = "2",
volume = "99",
doi = "10.1094/PDIS-07-14-0724-PDN"
}

Stanković, I., Milojević, K., Vučurović, A., Nikolić, D., Krstić, B.,& Bulajić, A.. (2015). First Report of Fusarium Root Rot of Stored Carrot Caused by Fusarium avenaceum in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 286-286.
https://doi.org/10.1094/PDIS-07-14-0724-PDN

Stanković I, Milojević K, Vučurović A, Nikolić D, Krstić B, Bulajić A. First Report of Fusarium Root Rot of Stored Carrot Caused by Fusarium avenaceum in Serbia.. in Plant Disease. 2015;99(2):286-286.
doi:10.1094/PDIS-07-14-0724-PDN .

Stanković, Ivana, Milojević, Katarina, Vučurović, Ana, Nikolić, D., Krstić, Branka, Bulajić, Aleksandra, "First Report of Fusarium Root Rot of Stored Carrot Caused by Fusarium avenaceum in Serbia." in Plant Disease, 99, no. 2 (2015):286-286,
https://doi.org/10.1094/PDIS-07-14-0724-PDN . .

TY  - JOUR
AU  - Milošević, D.
AU  - Marjanović-Jeromela, Ana
AU  - Ignjatov, Maja
AU  - Jovicić, D.
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3726
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip yellows virus on Oilseed Rape in Serbia
EP  - 1869
IS  - 12
SP  - 1869
VL  - 99
DO  - 10.1094/PDIS-05-15-0503-PDN
ER  - 

@article{
author = "Milošević, D. and Marjanović-Jeromela, Ana and Ignjatov, Maja and Jovicić, D. and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip yellows virus on Oilseed Rape in Serbia",
pages = "1869-1869",
number = "12",
volume = "99",
doi = "10.1094/PDIS-05-15-0503-PDN"
}

Milošević, D., Marjanović-Jeromela, A., Ignjatov, M., Jovicić, D., Stanković, I., Bulajić, A.,& Krstić, B.. (2015). First Report of Turnip yellows virus on Oilseed Rape in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(12), 1869-1869.
https://doi.org/10.1094/PDIS-05-15-0503-PDN

Milošević D, Marjanović-Jeromela A, Ignjatov M, Jovicić D, Stanković I, Bulajić A, Krstić B. First Report of Turnip yellows virus on Oilseed Rape in Serbia. in Plant Disease. 2015;99(12):1869-1869.
doi:10.1094/PDIS-05-15-0503-PDN .

Milošević, D., Marjanović-Jeromela, Ana, Ignjatov, Maja, Jovicić, D., Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, "First Report of Turnip yellows virus on Oilseed Rape in Serbia" in Plant Disease, 99, no. 12 (2015):1869-1869,
https://doi.org/10.1094/PDIS-05-15-0503-PDN . .

TY  - JOUR
AU  - Hrustić, Jovana
AU  - Delibašić, Goran
AU  - Stanković, Ivana
AU  - Grahovac, Mila
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Tanović, Brankica
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3790
AB  - Brown rot is one of the most important pre- and postharvest fungal diseases of stone fruit worldwide. In Serbia, where production of stone fruit is economically important, Monilinia laxa and M. fructigena are widely distributed. In surveys from 2011 to 2013, 288 isolates of Monilinia spp. were collected from 131 localities in 16 districts and from six hosts in Serbia. Using multiplex polymerase chain reaction, phylogenetic analysis, and morphological characterization, three species of Monilinia were identified as the causal agents of brown rot of stone fruit: M. laxa (89% of isolates), M. fructigena (3%), and M. fructicola (8%). In 2011, M. fructicola was reported for the first time on stone fruit in Serbia, with only one isolate detected. More isolates of M. fructicola were detected in 2012 (2 isolates) and 2013 (20 isolates). The presence of M. fructicola, as well as its increased frequency of detection during the survey, may indicate a change in the population structure of these pathogens, which could have an important impact on brown rot disease management in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia
EP  - 717
IS  - 5
SP  - 709
VL  - 99
DO  - 10.1094/PDIS-07-14-0732-RE
ER  - 

@article{
author = "Hrustić, Jovana and Delibašić, Goran and Stanković, Ivana and Grahovac, Mila and Krstić, Branka and Bulajić, Aleksandra and Tanović, Brankica",
year = "2015",
abstract = "Brown rot is one of the most important pre- and postharvest fungal diseases of stone fruit worldwide. In Serbia, where production of stone fruit is economically important, Monilinia laxa and M. fructigena are widely distributed. In surveys from 2011 to 2013, 288 isolates of Monilinia spp. were collected from 131 localities in 16 districts and from six hosts in Serbia. Using multiplex polymerase chain reaction, phylogenetic analysis, and morphological characterization, three species of Monilinia were identified as the causal agents of brown rot of stone fruit: M. laxa (89% of isolates), M. fructigena (3%), and M. fructicola (8%). In 2011, M. fructicola was reported for the first time on stone fruit in Serbia, with only one isolate detected. More isolates of M. fructicola were detected in 2012 (2 isolates) and 2013 (20 isolates). The presence of M. fructicola, as well as its increased frequency of detection during the survey, may indicate a change in the population structure of these pathogens, which could have an important impact on brown rot disease management in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia",
pages = "717-709",
number = "5",
volume = "99",
doi = "10.1094/PDIS-07-14-0732-RE"
}

Hrustić, J., Delibašić, G., Stanković, I., Grahovac, M., Krstić, B., Bulajić, A.,& Tanović, B.. (2015). Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 709-717.
https://doi.org/10.1094/PDIS-07-14-0732-RE

Hrustić J, Delibašić G, Stanković I, Grahovac M, Krstić B, Bulajić A, Tanović B. Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia. in Plant Disease. 2015;99(5):709-717.
doi:10.1094/PDIS-07-14-0732-RE .

Hrustić, Jovana, Delibašić, Goran, Stanković, Ivana, Grahovac, Mila, Krstić, Branka, Bulajić, Aleksandra, Tanović, Brankica, "Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia" in Plant Disease, 99, no. 5 (2015):709-717,
https://doi.org/10.1094/PDIS-07-14-0732-RE . .

TY  - CONF
AU  - Krstić, Branka
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Milojević, Katarina
AU  - Nikolić, D.M.
AU  - Teodorović, S.D.
AU  - Bulajić, Aleksandra
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3672
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Iris yellow spot virus epidemics in onion crops in Serbia
EP  - 76
IS  - 11
SP  - 76
VL  - 105
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3672
ER  - 

@conference{
author = "Krstić, Branka and Stanković, Ivana and Vučurović, Ana and Milojević, Katarina and Nikolić, D.M. and Teodorović, S.D. and Bulajić, Aleksandra",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Iris yellow spot virus epidemics in onion crops in Serbia",
pages = "76-76",
number = "11",
volume = "105",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3672"
}

Krstić, B., Stanković, I., Vučurović, A., Milojević, K., Nikolić, D.M., Teodorović, S.D.,& Bulajić, A.. (2015). Iris yellow spot virus epidemics in onion crops in Serbia. in Phytopathology
Amer Phytopathological Soc, St Paul., 105(11), 76-76.
https://hdl.handle.net/21.15107/rcub_agrospace_3672

Krstić B, Stanković I, Vučurović A, Milojević K, Nikolić D, Teodorović S, Bulajić A. Iris yellow spot virus epidemics in onion crops in Serbia. in Phytopathology. 2015;105(11):76-76.
https://hdl.handle.net/21.15107/rcub_agrospace_3672 .

Krstić, Branka, Stanković, Ivana, Vučurović, Ana, Milojević, Katarina, Nikolić, D.M., Teodorović, S.D., Bulajić, Aleksandra, "Iris yellow spot virus epidemics in onion crops in Serbia" in Phytopathology, 105, no. 11 (2015):76-76,
https://hdl.handle.net/21.15107/rcub_agrospace_3672 .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vrandečić, Karolina
AU  - Ćosić, Jasenka
AU  - Milojević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3630
AB  - A survey was conducted in 2012 and 2013 to detect the presence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. AMV was detected serologically in 62 samples collected at three different localities, and none of the samples tested positive for CMV. For further analyses, six selected samples of naturally infected lavandin plants originating from different localities were mechanically transmitted to test plants: Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana and Ocimum basilicum, confirming the infectious nature of the disease. Molecular detection was performed by amplification of a 751 bp fragment in all tested samples, using the specific primers CP AMV1/CP AMV2 that amplify the part of the coat protein (CP) gene and 3'-UTR. The RT-PCR products derived from the isolates 371-13 and 373-13 were sequenced (KJ504107 and KJ504108, respectively) and compared with the AMV sequences available in GenBank. CP sequence analysis, conducted using the MEGA5 software, revealed that the isolate 371-13 had the highest nucleotide identity of 99.5% (100% amino acid identity) with an isolate from Argentina originating from Medicago sativa (KC881010), while the sequence of isolate 373-13 had the highest identity with an Italian AMV isolate from Lavandula stoechas (FN667967) of 98.6% (99% amino acid identity). Phylogenetic analysis revealed the clustering of selected isolates into four molecular groups and the lavandin AMV isolates from Croatia grouped into two distinct groups, implying a significant variability within the AMV lavandin population.
AB  - Tokom 2012. i 2013. godine sprovedena su istraživanja u cilju utvrđivanja prisustva i rasprostranjenosti virusa mozaika lucerke (Alfalfa mosaic virus, AMV) u usevu lavande u kontinentalnom delu Hrvatske. Sa šest lokaliteta gajenja ukupno je sakupljeno 73 uzorka lavande koji su serološki testirani na prisustvo AMV i virusa mozaika krastavca (Cucumber mosaic virus, CMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo AMV dokazano je u 62 uzorka lavande sakupljena sa tri različita lokaliteta, dok prisustvo CMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka lavande poreklom iz različitih lokaliteta, koji su uspešno preneti mehaničkim inokulacijama na test biljke Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, i Ocimum basilicum, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 751 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CP AMV1/CP AMV2 koji omogućavaju umnožavanje dela gena za proteinski omotač i 3' neprepisujućeg regiona. U cilju dalje identifikacije, RT-PCR produkti izolata 371-13 i 373-13 su sekvencionisani (KJ504107 i KJ504108) i upoređeni sa AMV sekvencama dostupnim u GenBank bazi podataka. Analizom sekvenci gena za protein omotača, korišćenjem MEGA5 softverskog paketa, najviši stepen nukleotidne sličnosti od 99.7% (100% aminokiselinska sličnost) izolat 371-13 pokazao je sa izolatom AMV iz Argentine poreklom iz Medicago sativa (KC881010), dok sekvenca izolata 373-13 najveću sličnost od 98.6% (99% aminokiselinska sličnost) deli sa italijanskim izolatom AMV iz Lavandula stoechas (FN667967). Filogenetska analiza pokazala je grupisanje izolata u četiri molekularne grupe, a izolati AMV iz lavande iz Hrvatske grupišu se u dve odvojene grupe, ukazujući na postojanje značajne varijabilnosti u populaciji AMV poreklom iz lavande.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The spreading of alfalfa mosaic virus in lavandin in Croatia
T1  - Širenje virusa mozaika lucerke u usevu lavande u Hrvatskoj
EP  - 122
IS  - 2
SP  - 115
VL  - 29
DO  - 10.2298/PIF1402115S
ER  - 

@article{
author = "Stanković, Ivana and Vrandečić, Karolina and Ćosić, Jasenka and Milojević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2014",
abstract = "A survey was conducted in 2012 and 2013 to detect the presence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. AMV was detected serologically in 62 samples collected at three different localities, and none of the samples tested positive for CMV. For further analyses, six selected samples of naturally infected lavandin plants originating from different localities were mechanically transmitted to test plants: Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana and Ocimum basilicum, confirming the infectious nature of the disease. Molecular detection was performed by amplification of a 751 bp fragment in all tested samples, using the specific primers CP AMV1/CP AMV2 that amplify the part of the coat protein (CP) gene and 3'-UTR. The RT-PCR products derived from the isolates 371-13 and 373-13 were sequenced (KJ504107 and KJ504108, respectively) and compared with the AMV sequences available in GenBank. CP sequence analysis, conducted using the MEGA5 software, revealed that the isolate 371-13 had the highest nucleotide identity of 99.5% (100% amino acid identity) with an isolate from Argentina originating from Medicago sativa (KC881010), while the sequence of isolate 373-13 had the highest identity with an Italian AMV isolate from Lavandula stoechas (FN667967) of 98.6% (99% amino acid identity). Phylogenetic analysis revealed the clustering of selected isolates into four molecular groups and the lavandin AMV isolates from Croatia grouped into two distinct groups, implying a significant variability within the AMV lavandin population., Tokom 2012. i 2013. godine sprovedena su istraživanja u cilju utvrđivanja prisustva i rasprostranjenosti virusa mozaika lucerke (Alfalfa mosaic virus, AMV) u usevu lavande u kontinentalnom delu Hrvatske. Sa šest lokaliteta gajenja ukupno je sakupljeno 73 uzorka lavande koji su serološki testirani na prisustvo AMV i virusa mozaika krastavca (Cucumber mosaic virus, CMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo AMV dokazano je u 62 uzorka lavande sakupljena sa tri različita lokaliteta, dok prisustvo CMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka lavande poreklom iz različitih lokaliteta, koji su uspešno preneti mehaničkim inokulacijama na test biljke Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, i Ocimum basilicum, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 751 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CP AMV1/CP AMV2 koji omogućavaju umnožavanje dela gena za proteinski omotač i 3' neprepisujućeg regiona. U cilju dalje identifikacije, RT-PCR produkti izolata 371-13 i 373-13 su sekvencionisani (KJ504107 i KJ504108) i upoređeni sa AMV sekvencama dostupnim u GenBank bazi podataka. Analizom sekvenci gena za protein omotača, korišćenjem MEGA5 softverskog paketa, najviši stepen nukleotidne sličnosti od 99.7% (100% aminokiselinska sličnost) izolat 371-13 pokazao je sa izolatom AMV iz Argentine poreklom iz Medicago sativa (KC881010), dok sekvenca izolata 373-13 najveću sličnost od 98.6% (99% aminokiselinska sličnost) deli sa italijanskim izolatom AMV iz Lavandula stoechas (FN667967). Filogenetska analiza pokazala je grupisanje izolata u četiri molekularne grupe, a izolati AMV iz lavande iz Hrvatske grupišu se u dve odvojene grupe, ukazujući na postojanje značajne varijabilnosti u populaciji AMV poreklom iz lavande.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The spreading of alfalfa mosaic virus in lavandin in Croatia, Širenje virusa mozaika lucerke u usevu lavande u Hrvatskoj",
pages = "122-115",
number = "2",
volume = "29",
doi = "10.2298/PIF1402115S"
}

Stanković, I., Vrandečić, K., Ćosić, J., Milojević, K., Bulajić, A.,& Krstić, B.. (2014). The spreading of alfalfa mosaic virus in lavandin in Croatia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(2), 115-122.
https://doi.org/10.2298/PIF1402115S

Stanković I, Vrandečić K, Ćosić J, Milojević K, Bulajić A, Krstić B. The spreading of alfalfa mosaic virus in lavandin in Croatia. in Pesticidi i fitomedicina. 2014;29(2):115-122.
doi:10.2298/PIF1402115S .

Stanković, Ivana, Vrandečić, Karolina, Ćosić, Jasenka, Milojević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "The spreading of alfalfa mosaic virus in lavandin in Croatia" in Pesticidi i fitomedicina, 29, no. 2 (2014):115-122,
https://doi.org/10.2298/PIF1402115S . .