TY  - JOUR
AU  - Matović, Kazimir
AU  - Misić, Dušan
AU  - Karabasil, Nedjeljko
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Jevtić, Goran
AU  - Ćirić, Jelena
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5064
AB  - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Clostridium botulinum spores in European honey bees from Serbia
EP  - 426
IS  - 3
SP  - 420
VL  - 58
DO  - 10.1080/00218839.2018.1560654
ER  - 

@article{
author = "Matović, Kazimir and Misić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena",
year = "2019",
abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Clostridium botulinum spores in European honey bees from Serbia",
pages = "426-420",
number = "3",
volume = "58",
doi = "10.1080/00218839.2018.1560654"
}

Matović, K., Misić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 58(3), 420-426.
https://doi.org/10.1080/00218839.2018.1560654

Matović K, Misić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426.
doi:10.1080/00218839.2018.1560654 .

Matović, Kazimir, Misić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426,
https://doi.org/10.1080/00218839.2018.1560654 . .

TY  - CONF
AU  - Matović, Kazimir
AU  - Baltić, Milan Ž.
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Nenadić, Dragan
AU  - Vasković, Nikola
AU  - Jevtić, Goran
AU  - Misić, Dušan
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3737
AB  - The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.
PB  - Elsevier Science Bv, Amsterdam
C3  - 58th International Meat Industry Conference (Meatcon2015)
T1  - The investigation of the presence of Clostridium botulinum spores in honey in Serbia
EP  - 183
SP  - 180
VL  - 5
DO  - 10.1016/j.profoo.2015.09.051
ER  - 

@conference{
author = "Matović, Kazimir and Baltić, Milan Ž. and Nedić, Nebojša and Dmitrić, Marko and Nenadić, Dragan and Vasković, Nikola and Jevtić, Goran and Misić, Dušan",
year = "2015",
abstract = "The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "58th International Meat Industry Conference (Meatcon2015)",
title = "The investigation of the presence of Clostridium botulinum spores in honey in Serbia",
pages = "183-180",
volume = "5",
doi = "10.1016/j.profoo.2015.09.051"
}

Matović, K., Baltić, M. Ž., Nedić, N., Dmitrić, M., Nenadić, D., Vasković, N., Jevtić, G.,& Misić, D.. (2015). The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (Meatcon2015)
Elsevier Science Bv, Amsterdam., 5, 180-183.
https://doi.org/10.1016/j.profoo.2015.09.051

Matović K, Baltić MŽ, Nedić N, Dmitrić M, Nenadić D, Vasković N, Jevtić G, Misić D. The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (Meatcon2015). 2015;5:180-183.
doi:10.1016/j.profoo.2015.09.051 .

Matović, Kazimir, Baltić, Milan Ž., Nedić, Nebojša, Dmitrić, Marko, Nenadić, Dragan, Vasković, Nikola, Jevtić, Goran, Misić, Dušan, "The investigation of the presence of Clostridium botulinum spores in honey in Serbia" in 58th International Meat Industry Conference (Meatcon2015), 5 (2015):180-183,
https://doi.org/10.1016/j.profoo.2015.09.051 . .