TY  - JOUR
AU  - Krivokapić, Marija
AU  - Gavrilović, Veljko
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Fira, Djordje
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4901
AB  - The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
AB  - Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia
T1  - Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji
EP  - 184
IS  - 3-4
SP  - 175
VL  - 33
DO  - 10.2298/PIF1804175K
ER  - 

@article{
author = "Krivokapić, Marija and Gavrilović, Veljko and Ivanović, Milan and Kuzmanović, Nemanja and Fira, Djordje and Obradović, Aleksa and Gašić, Katarina",
year = "2018",
abstract = "The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation., Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia, Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji",
pages = "184-175",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804175K"
}

Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, D., Obradović, A.,& Gašić, K.. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(3-4), 175-184.
https://doi.org/10.2298/PIF1804175K

Krivokapić M, Gavrilović V, Ivanović M, Kuzmanović N, Fira D, Obradović A, Gašić K. Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina. 2018;33(3-4):175-184.
doi:10.2298/PIF1804175K .

Krivokapić, Marija, Gavrilović, Veljko, Ivanović, Milan, Kuzmanović, Nemanja, Fira, Djordje, Obradović, Aleksa, Gašić, Katarina, "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):175-184,
https://doi.org/10.2298/PIF1804175K . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Momirović, Nebojša
AU  - Stanisavljević, Rade
AU  - Štrbanović, Ratibor
AU  - Gavrilović, Veljko
AU  - Aleksić, Goran
AU  - Djukanović, Lana
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3624
AB  - Examination is seed quality of perennial ryegrass (Lolium perenne), italian ryegrass (Lolium multiflorum) and red fescue (Festuca rubra) during the five years 2010 - 2014. The average established seed purity was over 98.40%, with varying by year from 96.7% to 99.2%. The greatest part of the impurities was inert matter, which are varied over the years in the range from 0.0% to 2.30%. Presence of weed seeds by year varied in the range from 0.0% to 0.50%. In the seeds of the tested grasses dominate the weeds from the family Asteraceae, Caryophillaceae, Chenopodiaceae and Polygonaceae. The most common weeds in the investigated grasses were Amaranthus retroflexus and Chenopodium album. Participation of other species by year ranged from 0.0% to 1.8%. Seed germination varied from 75 to 88%, the average was 83%. The number of abnormal seedlings was 4%, while the average number of ungerminated seeds was 13%.
AB  - Ispitivanjem semena engleskog ljulja (Lolium perenne), italijanskog ljulja (Lolium multi­florum) i crvenog vijuka (Festuca rubra) u toku pet godina (2010 - 2014. godina) utvrđene su vrlo visoke vrednosti svih značajnih pokazatelja kvaliteta semena. Prosečna utvrđena čistoća semena bila je preko 98,40%, sa variranjem po godinama od 96,7 % do 99,2 %. Pri tome najveći deo primesa činile su inertne materije, koje su varirale po godinama u intervalu od 0,0 % do 2,30 %. Prisustvo semena korova po godinama je variralo u intervalu od 0,0 % do 0,50 %. U semenu ispitivanih trava dominiraju korovi iz familija Asteraceae, Caryophillaceae, Chenopodiaceae i Polygonaceae. Najzastupljeniji korovi u ispitivanim travama su Amaranthus retroflexus (obični štir) i Chenopodium album (obična pepeljuga). Učešće drugih vrsta po godinama kretalo se u intervalu od 0,0 % do 1,8 %. Klijavost semena varirala je od 75 do 88%, prosečno je iznosila 83%. Broj nenormalnih klijanaca bio je 4%, dok je prosečan broj neklijalog semena bio 13%.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Examination quality seed perennial ryegrass, Italian ryegrass and red fescue
T1  - Ispitivanje kvaliteta semena engleskog ljulja, italijanskog ljulja i crvenog vijuka
EP  - 76
IS  - 2
SP  - 70
VL  - 65
DO  - 10.5937/zasbilj1402070P
ER  - 

@article{
author = "Poštić, Dobrivoj and Momirović, Nebojša and Stanisavljević, Rade and Štrbanović, Ratibor and Gavrilović, Veljko and Aleksić, Goran and Djukanović, Lana",
year = "2014",
abstract = "Examination is seed quality of perennial ryegrass (Lolium perenne), italian ryegrass (Lolium multiflorum) and red fescue (Festuca rubra) during the five years 2010 - 2014. The average established seed purity was over 98.40%, with varying by year from 96.7% to 99.2%. The greatest part of the impurities was inert matter, which are varied over the years in the range from 0.0% to 2.30%. Presence of weed seeds by year varied in the range from 0.0% to 0.50%. In the seeds of the tested grasses dominate the weeds from the family Asteraceae, Caryophillaceae, Chenopodiaceae and Polygonaceae. The most common weeds in the investigated grasses were Amaranthus retroflexus and Chenopodium album. Participation of other species by year ranged from 0.0% to 1.8%. Seed germination varied from 75 to 88%, the average was 83%. The number of abnormal seedlings was 4%, while the average number of ungerminated seeds was 13%., Ispitivanjem semena engleskog ljulja (Lolium perenne), italijanskog ljulja (Lolium multi­florum) i crvenog vijuka (Festuca rubra) u toku pet godina (2010 - 2014. godina) utvrđene su vrlo visoke vrednosti svih značajnih pokazatelja kvaliteta semena. Prosečna utvrđena čistoća semena bila je preko 98,40%, sa variranjem po godinama od 96,7 % do 99,2 %. Pri tome najveći deo primesa činile su inertne materije, koje su varirale po godinama u intervalu od 0,0 % do 2,30 %. Prisustvo semena korova po godinama je variralo u intervalu od 0,0 % do 0,50 %. U semenu ispitivanih trava dominiraju korovi iz familija Asteraceae, Caryophillaceae, Chenopodiaceae i Polygonaceae. Najzastupljeniji korovi u ispitivanim travama su Amaranthus retroflexus (obični štir) i Chenopodium album (obična pepeljuga). Učešće drugih vrsta po godinama kretalo se u intervalu od 0,0 % do 1,8 %. Klijavost semena varirala je od 75 do 88%, prosečno je iznosila 83%. Broj nenormalnih klijanaca bio je 4%, dok je prosečan broj neklijalog semena bio 13%.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Examination quality seed perennial ryegrass, Italian ryegrass and red fescue, Ispitivanje kvaliteta semena engleskog ljulja, italijanskog ljulja i crvenog vijuka",
pages = "76-70",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402070P"
}

Poštić, D., Momirović, N., Stanisavljević, R., Štrbanović, R., Gavrilović, V., Aleksić, G.,& Djukanović, L.. (2014). Examination quality seed perennial ryegrass, Italian ryegrass and red fescue. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(2), 70-76.
https://doi.org/10.5937/zasbilj1402070P

Poštić D, Momirović N, Stanisavljević R, Štrbanović R, Gavrilović V, Aleksić G, Djukanović L. Examination quality seed perennial ryegrass, Italian ryegrass and red fescue. in Zaštita bilja. 2014;65(2):70-76.
doi:10.5937/zasbilj1402070P .

Poštić, Dobrivoj, Momirović, Nebojša, Stanisavljević, Rade, Štrbanović, Ratibor, Gavrilović, Veljko, Aleksić, Goran, Djukanović, Lana, "Examination quality seed perennial ryegrass, Italian ryegrass and red fescue" in Zaštita bilja, 65, no. 2 (2014):70-76,
https://doi.org/10.5937/zasbilj1402070P . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3556
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Gašić, K.
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Obradović, Aleksa
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3130
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Broccoli Soft Rot Caused by Pectobacterium carotovorum subsp carotovorum in Serbia.
EP  - 1504
IS  - 11
SP  - 1504
VL  - 97
DO  - 10.1094/PDIS-03-13-0258-PDN
ER  - 

@article{
author = "Gašić, K. and Gavrilović, Veljko and Ivanović, Žarko and Obradović, Aleksa",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Broccoli Soft Rot Caused by Pectobacterium carotovorum subsp carotovorum in Serbia.",
pages = "1504-1504",
number = "11",
volume = "97",
doi = "10.1094/PDIS-03-13-0258-PDN"
}

Gašić, K., Gavrilović, V., Ivanović, Ž.,& Obradović, A.. (2013). First Report of Broccoli Soft Rot Caused by Pectobacterium carotovorum subsp carotovorum in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(11), 1504-1504.
https://doi.org/10.1094/PDIS-03-13-0258-PDN

Gašić K, Gavrilović V, Ivanović Ž, Obradović A. First Report of Broccoli Soft Rot Caused by Pectobacterium carotovorum subsp carotovorum in Serbia.. in Plant Disease. 2013;97(11):1504-1504.
doi:10.1094/PDIS-03-13-0258-PDN .

Gašić, K., Gavrilović, Veljko, Ivanović, Žarko, Obradović, Aleksa, "First Report of Broccoli Soft Rot Caused by Pectobacterium carotovorum subsp carotovorum in Serbia." in Plant Disease, 97, no. 11 (2013):1504-1504,
https://doi.org/10.1094/PDIS-03-13-0258-PDN . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Momirović, Nebojša
AU  - Dolijanović, Željko
AU  - Broćić, Zoran
AU  - Štrbanović, Ratibor
AU  - Popović, Tatjana
AU  - Gavrilović, Veljko
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2482
AB  - The aim of this study was to determine the impact of the size of tubers originating form different altitudes, on the yield of most commonly grown potato cultivars Cleopatra, Jaerla, Desiree and Kennebec in western Serbia (Macva). Potato seed-producing crop, from which the planting material came from, was grown during 2006 at two sites with different altitude: a plain region 72m a.s.l. and a mountainous region 1100m a.s.l. Evaluating the presence of the two most widespread potato virus (PYV) and (PLRV) in our conditions was performed by ELISA test. The investigation was carried out by planting the following mass of planted material: 50 ± 5g, 70 ± 5g, 90 ± 5g and 110 ± 5g in 2007 year. Higher yields of potato tubers in moist conditions in western Serbia (Macva) was achieved by planting larger mass of tubers originating from the lower altitude and shorter growing season varieties.
AB  - Cilj rada je bio da se utvrdi uticaj porekla sadnog materijala i veličine semenske krtole na produktivne osobine četiri najčešće gajene sorte krompira Cleopatra, Jaerla, Desiree i Kennebec u uslovima zapadne Srbije (Mačve). Proizvodnja sadnog materijala krompira izvedena je 2006. godine u dva lokaliteta različite nadmorske visine (nv): ravničarskom 72m i planinskom regionu 1100m (nv). Ocena prisustva dva i najraširenija virusa krompira u našim uslovima (PYV) i (PLRV) izvršeno je ELISA testom. Istraživanja su izvedena sadnjom semenskih krtola mase 50 ± 5g, 70 ± 5g, 90 ± 5g i 110 ± 5g tokom 2007. godine. Najveći ukupan prinos krtola od 36,10 t/ha־¹ ostvarila je rana sorta Cleopatra na varijantama gde je sađena najkrupnija frakcije (110g) poreklo sa planinskog područja. Veći prinosi krtola krompira u uslovima prirodnog vodnog režima zapadne Srbije (Mačve) postižu se sadnjom zdravih semenskih krtola veće mase poreklom sa ravničarskog regiona i sorti kraće vegetacije.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - The effect of the size of potato tubers originating from different altitudes on the productive properties of potato
T1  - Uticaj porekla sadnog materijala i veličine semenske krtole na produktivne osobine krompira
EP  - 146
IS  - 2
SP  - 135
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2482
ER  - 

@article{
author = "Poštić, Dobrivoj and Momirović, Nebojša and Dolijanović, Željko and Broćić, Zoran and Štrbanović, Ratibor and Popović, Tatjana and Gavrilović, Veljko",
year = "2011",
abstract = "The aim of this study was to determine the impact of the size of tubers originating form different altitudes, on the yield of most commonly grown potato cultivars Cleopatra, Jaerla, Desiree and Kennebec in western Serbia (Macva). Potato seed-producing crop, from which the planting material came from, was grown during 2006 at two sites with different altitude: a plain region 72m a.s.l. and a mountainous region 1100m a.s.l. Evaluating the presence of the two most widespread potato virus (PYV) and (PLRV) in our conditions was performed by ELISA test. The investigation was carried out by planting the following mass of planted material: 50 ± 5g, 70 ± 5g, 90 ± 5g and 110 ± 5g in 2007 year. Higher yields of potato tubers in moist conditions in western Serbia (Macva) was achieved by planting larger mass of tubers originating from the lower altitude and shorter growing season varieties., Cilj rada je bio da se utvrdi uticaj porekla sadnog materijala i veličine semenske krtole na produktivne osobine četiri najčešće gajene sorte krompira Cleopatra, Jaerla, Desiree i Kennebec u uslovima zapadne Srbije (Mačve). Proizvodnja sadnog materijala krompira izvedena je 2006. godine u dva lokaliteta različite nadmorske visine (nv): ravničarskom 72m i planinskom regionu 1100m (nv). Ocena prisustva dva i najraširenija virusa krompira u našim uslovima (PYV) i (PLRV) izvršeno je ELISA testom. Istraživanja su izvedena sadnjom semenskih krtola mase 50 ± 5g, 70 ± 5g, 90 ± 5g i 110 ± 5g tokom 2007. godine. Najveći ukupan prinos krtola od 36,10 t/ha־¹ ostvarila je rana sorta Cleopatra na varijantama gde je sađena najkrupnija frakcije (110g) poreklo sa planinskog područja. Veći prinosi krtola krompira u uslovima prirodnog vodnog režima zapadne Srbije (Mačve) postižu se sadnjom zdravih semenskih krtola veće mase poreklom sa ravničarskog regiona i sorti kraće vegetacije.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "The effect of the size of potato tubers originating from different altitudes on the productive properties of potato, Uticaj porekla sadnog materijala i veličine semenske krtole na produktivne osobine krompira",
pages = "146-135",
number = "2",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2482"
}

Poštić, D., Momirović, N., Dolijanović, Ž., Broćić, Z., Štrbanović, R., Popović, T.,& Gavrilović, V.. (2011). The effect of the size of potato tubers originating from different altitudes on the productive properties of potato. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 62(2), 135-146.
https://hdl.handle.net/21.15107/rcub_agrospace_2482

Poštić D, Momirović N, Dolijanović Ž, Broćić Z, Štrbanović R, Popović T, Gavrilović V. The effect of the size of potato tubers originating from different altitudes on the productive properties of potato. in Zaštita bilja. 2011;62(2):135-146.
https://hdl.handle.net/21.15107/rcub_agrospace_2482 .

Poštić, Dobrivoj, Momirović, Nebojša, Dolijanović, Željko, Broćić, Zoran, Štrbanović, Ratibor, Popović, Tatjana, Gavrilović, Veljko, "The effect of the size of potato tubers originating from different altitudes on the productive properties of potato" in Zaštita bilja, 62, no. 2 (2011):135-146,
https://hdl.handle.net/21.15107/rcub_agrospace_2482 .

TY  - CONF
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Obradović, Aleksa
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
AU  - Gavrilović, Veljko
AU  - Balaž, Jelica
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2441
AB  - A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.
C3  - Acta Horticulturae
T1  - Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG
EP  - 72
SP  - 65
VL  - 896
DO  - 10.17660/actahortic.2011.896.6
ER  - 

@conference{
author = "Ivanović, Milan and Gašić, K. and Obradović, Aleksa and Dickstein, Ellen R. and Jones, Jeffrey B. and Gavrilović, Veljko and Balaž, Jelica",
year = "2011",
abstract = "A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.",
journal = "Acta Horticulturae",
title = "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG",
pages = "72-65",
volume = "896",
doi = "10.17660/actahortic.2011.896.6"
}

Ivanović, M., Gašić, K., Obradović, A., Dickstein, E. R., Jones, J. B., Gavrilović, V.,& Balaž, J.. (2011). Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in Acta Horticulturae, 896, 65-72.
https://doi.org/10.17660/actahortic.2011.896.6

Ivanović M, Gašić K, Obradović A, Dickstein ER, Jones JB, Gavrilović V, Balaž J. Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in Acta Horticulturae. 2011;896:65-72.
doi:10.17660/actahortic.2011.896.6 .

Ivanović, Milan, Gašić, K., Obradović, Aleksa, Dickstein, Ellen R., Jones, Jeffrey B., Gavrilović, Veljko, Balaž, Jelica, "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG" in Acta Horticulturae, 896 (2011):65-72,
https://doi.org/10.17660/actahortic.2011.896.6 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Obradović, Aleksa
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1954
AB  - Bacterial strains were isolated from above- and underground parts of diseased calla plants originating from different localities in Serbia and one locality in Montenegro. They were characterized by studying their pathogenic, cultural, biochemical and physiological characteristics. All investigated strains caused soft rot of calla leaf stalks, potato slices and aloe leaves, and induced hypersensitive reaction on tobacco. Bacteriological properties of the strains indicated that symptoms on calla plants were caused by Gram-negative, nonfluorescent, oxidase negative, catalase positive and facultatively anaerobic bacterium belonging to the genus Pectobacterium. The investigated strains grew at 37ºC and in 5% NaCl, utilised lactose and trechalose, and produced neither indol nor lecitinase. These results, as well as the characteristic growth on Logan's differential medium indicated that soft rot of tuber and stem base of calla plants was caused by Pectobacterium carotovorum subsp. carotovorum. This is the first report of this pathogen affecting calla plants in Serbia.
AB  - Proučene su patogene, odgajivačke i biohemijsko-fiziološke odlike sojeva izolovanih iz nadzemnih i podzemnih delova obolelih biljaka kale gajene u različitim lokalitetima u Srbiji i jednom lokalitetu u Crnoj Gori. Svi proučavani sojevi ispoljili su izraženu pektolitičku aktivnost prouzrokujući vlažnu trulež lisnih drški kale, kriški krompira, liski aloje ili sanseverije, a prouzrokovali su i hipersenzitivnu reakciju duvana. Proučavanjem bakterioloških karakteristika utvrđeno je da promene na biljkama kale prouzrokuju Gram-negativni, nefluorescentni, oksidaza-negativni, katalaza-pozitivni i fakultativno-anaerobni sojevi bakterije, koja prema navedenim karakteristikama pripada rodu Pectobacterium. Proučavani sojevi se razvijaju pri 37ºC i u prisustvu 5% NaCl, razlažu laktozu i trehalozu, ne stvaraju indol i lecitinazu. Navedeni rezultati, kao i karakterističan razvoj na Loganovoj diferencijalnoj podlozi ukazuju da je vlažnu trulež korena i prizemnog dela biljaka kale prouzrokovala bakterija Pectobacterium carotovorum subsp. carotovorum. Ovo je prvo saopštenje o pojavi P. c. ssp. carotovorum kao patogena kale u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. Carotovorum: The causal agent of calla soft rot in Serbia and Montenegro
T1  - Pectobacterium carotovorum subsp. Carotovorum - prouzrokovač vlažne truleži biljaka kale u Srbiji i Crnoj Gori
EP  - 293
IS  - 4
SP  - 287
VL  - 24
DO  - 10.2298/PIF0904287I
ER  - 

@article{
author = "Ivanović, Milan and Gašić, Katarina and Gavrilović, Veljko and Obradović, Aleksa",
year = "2009",
abstract = "Bacterial strains were isolated from above- and underground parts of diseased calla plants originating from different localities in Serbia and one locality in Montenegro. They were characterized by studying their pathogenic, cultural, biochemical and physiological characteristics. All investigated strains caused soft rot of calla leaf stalks, potato slices and aloe leaves, and induced hypersensitive reaction on tobacco. Bacteriological properties of the strains indicated that symptoms on calla plants were caused by Gram-negative, nonfluorescent, oxidase negative, catalase positive and facultatively anaerobic bacterium belonging to the genus Pectobacterium. The investigated strains grew at 37ºC and in 5% NaCl, utilised lactose and trechalose, and produced neither indol nor lecitinase. These results, as well as the characteristic growth on Logan's differential medium indicated that soft rot of tuber and stem base of calla plants was caused by Pectobacterium carotovorum subsp. carotovorum. This is the first report of this pathogen affecting calla plants in Serbia., Proučene su patogene, odgajivačke i biohemijsko-fiziološke odlike sojeva izolovanih iz nadzemnih i podzemnih delova obolelih biljaka kale gajene u različitim lokalitetima u Srbiji i jednom lokalitetu u Crnoj Gori. Svi proučavani sojevi ispoljili su izraženu pektolitičku aktivnost prouzrokujući vlažnu trulež lisnih drški kale, kriški krompira, liski aloje ili sanseverije, a prouzrokovali su i hipersenzitivnu reakciju duvana. Proučavanjem bakterioloških karakteristika utvrđeno je da promene na biljkama kale prouzrokuju Gram-negativni, nefluorescentni, oksidaza-negativni, katalaza-pozitivni i fakultativno-anaerobni sojevi bakterije, koja prema navedenim karakteristikama pripada rodu Pectobacterium. Proučavani sojevi se razvijaju pri 37ºC i u prisustvu 5% NaCl, razlažu laktozu i trehalozu, ne stvaraju indol i lecitinazu. Navedeni rezultati, kao i karakterističan razvoj na Loganovoj diferencijalnoj podlozi ukazuju da je vlažnu trulež korena i prizemnog dela biljaka kale prouzrokovala bakterija Pectobacterium carotovorum subsp. carotovorum. Ovo je prvo saopštenje o pojavi P. c. ssp. carotovorum kao patogena kale u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. Carotovorum: The causal agent of calla soft rot in Serbia and Montenegro, Pectobacterium carotovorum subsp. Carotovorum - prouzrokovač vlažne truleži biljaka kale u Srbiji i Crnoj Gori",
pages = "293-287",
number = "4",
volume = "24",
doi = "10.2298/PIF0904287I"
}

Ivanović, M., Gašić, K., Gavrilović, V.,& Obradović, A.. (2009). Pectobacterium carotovorum subsp. Carotovorum: The causal agent of calla soft rot in Serbia and Montenegro. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(4), 287-293.
https://doi.org/10.2298/PIF0904287I

Ivanović M, Gašić K, Gavrilović V, Obradović A. Pectobacterium carotovorum subsp. Carotovorum: The causal agent of calla soft rot in Serbia and Montenegro. in Pesticidi i fitomedicina. 2009;24(4):287-293.
doi:10.2298/PIF0904287I .

Ivanović, Milan, Gašić, Katarina, Gavrilović, Veljko, Obradović, Aleksa, "Pectobacterium carotovorum subsp. Carotovorum: The causal agent of calla soft rot in Serbia and Montenegro" in Pesticidi i fitomedicina, 24, no. 4 (2009):287-293,
https://doi.org/10.2298/PIF0904287I . .

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Obradović, Aleksa
AU  - Milijašević, Svetlana
AU  - Zivković, S.
AU  - Arsenijević, M.
AU  - Vojinović, M.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1784
AB  - In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - Proceedings of the Eleventh International Workshop on Fire Blight
T1  - Sorbus sp - New host of Erwinia amylovora in Serbia
EP  - +
SP  - 351
VL  - 793
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1784
ER  - 

@conference{
author = "Gavrilović, Veljko and Obradović, Aleksa and Milijašević, Svetlana and Zivković, S. and Arsenijević, M. and Vojinović, M.",
year = "2008",
abstract = "In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Proceedings of the Eleventh International Workshop on Fire Blight",
title = "Sorbus sp - New host of Erwinia amylovora in Serbia",
pages = "+-351",
volume = "793",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1784"
}

Gavrilović, V., Obradović, A., Milijašević, S., Zivković, S., Arsenijević, M.,& Vojinović, M.. (2008). Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 793, 351-+.
https://hdl.handle.net/21.15107/rcub_agrospace_1784

Gavrilović V, Obradović A, Milijašević S, Zivković S, Arsenijević M, Vojinović M. Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight. 2008;793:351-+.
https://hdl.handle.net/21.15107/rcub_agrospace_1784 .

Gavrilović, Veljko, Obradović, Aleksa, Milijašević, Svetlana, Zivković, S., Arsenijević, M., Vojinović, M., "Sorbus sp - New host of Erwinia amylovora in Serbia" in Proceedings of the Eleventh International Workshop on Fire Blight, 793 (2008):351-+,
https://hdl.handle.net/21.15107/rcub_agrospace_1784 .

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Živković, S.
AU  - Obradović, Aleksa
AU  - Milijašević, Svetlana
AU  - Arsenijević, M.
AU  - Vojinović, M.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1633
AB  - In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanomeles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25°C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.
PB  - 11th International Workshop on Fire Blight
C3  - Acta Horticulturae
T1  - Sorbus sp. - New host of Erwinia amylovora in Serbia
EP  - 356
SP  - 351
VL  - 793
DO  - 10.17660/actahortic.2008.793.52
ER  - 

@conference{
author = "Gavrilović, Veljko and Živković, S. and Obradović, Aleksa and Milijašević, Svetlana and Arsenijević, M. and Vojinović, M.",
year = "2008",
abstract = "In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanomeles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25°C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.",
publisher = "11th International Workshop on Fire Blight",
journal = "Acta Horticulturae",
title = "Sorbus sp. - New host of Erwinia amylovora in Serbia",
pages = "356-351",
volume = "793",
doi = "10.17660/actahortic.2008.793.52"
}

Gavrilović, V., Živković, S., Obradović, A., Milijašević, S., Arsenijević, M.,& Vojinović, M.. (2008). Sorbus sp. - New host of Erwinia amylovora in Serbia. in Acta Horticulturae
11th International Workshop on Fire Blight., 793, 351-356.
https://doi.org/10.17660/actahortic.2008.793.52

Gavrilović V, Živković S, Obradović A, Milijašević S, Arsenijević M, Vojinović M. Sorbus sp. - New host of Erwinia amylovora in Serbia. in Acta Horticulturae. 2008;793:351-356.
doi:10.17660/actahortic.2008.793.52 .

Gavrilović, Veljko, Živković, S., Obradović, Aleksa, Milijašević, Svetlana, Arsenijević, M., Vojinović, M., "Sorbus sp. - New host of Erwinia amylovora in Serbia" in Acta Horticulturae, 793 (2008):351-356,
https://doi.org/10.17660/actahortic.2008.793.52 . .

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Obradović, Aleksa
AU  - Arsenijević, M.
PY  - 2001
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/333
PB  - Springer, Dordrecht
C3  - Plant Pathogenic Bacteria
T1  - Bacterial soft rot of carrot, parsley and celery
EP  - 271
SP  - 269
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_333
ER  - 

@conference{
author = "Gavrilović, Veljko and Obradović, Aleksa and Arsenijević, M.",
year = "2001",
publisher = "Springer, Dordrecht",
journal = "Plant Pathogenic Bacteria",
title = "Bacterial soft rot of carrot, parsley and celery",
pages = "271-269",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_333"
}

Gavrilović, V., Obradović, A.,& Arsenijević, M.. (2001). Bacterial soft rot of carrot, parsley and celery. in Plant Pathogenic Bacteria
Springer, Dordrecht., 269-271.
https://hdl.handle.net/21.15107/rcub_agrospace_333

Gavrilović V, Obradović A, Arsenijević M. Bacterial soft rot of carrot, parsley and celery. in Plant Pathogenic Bacteria. 2001;:269-271.
https://hdl.handle.net/21.15107/rcub_agrospace_333 .

Gavrilović, Veljko, Obradović, Aleksa, Arsenijević, M., "Bacterial soft rot of carrot, parsley and celery" in Plant Pathogenic Bacteria (2001):269-271,
https://hdl.handle.net/21.15107/rcub_agrospace_333 .