TY  - JOUR
AU  - Molerović, Nikola
AU  - Rašković, Božidar
AU  - Djedović, Radica
AU  - Ostojić-Andrić, Dušica
AU  - Marković, Zoran
AU  - Marić, Saša
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5196
AB  - Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock.
AB  - U cilju zaštite ţivotne sredine i genetiĉkih resursa potoĉne pastrmke (Salmo trutta) u radu je ispitivana genetiĉka struktura ove vrste na ribnjaku ―Braduljica‖ u blizini Ivanjice. DNK je izolovana iz odseĉaka peraja 10 jedinki, a nakon toga molekularne PCR-RFLP metode su korišćene za razlikovanje izmeĊu atlantske i dunavske linije na bazi kontrolnog regiona mitohondrijalne DNK (KR mtDNK) i jedarnog gena za laktat dehidrogenazu (LDH nDNK). Na osnovu fenotipskih karakteristika procenjeno je da je pet jedinki pripadalo alohtonoj atlantskoj liniji, a preostalih pet autohtonoj dunavskoj liniji potoĉne pastrmke. MeĊutim, rezultati molekularnih analiza pokazali su prisustvo visokog procenta alohtonih gena u analiziranom uzorku, što potvrĊuje hibridizaciju izmeĊu ove dve linije. TakoĊe, rezultati ukazuju na to da selekcija na osnovu fenotipa nije adekvatna. Kako bi ribnjak nastavio da se bavi gajenjem potoĉne pastrmke za potrebe poribljavanja okolnih reka, neophodno je da se eliminišu jedinke atlantske linije iz matiĉnog fonda.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia
T1  - Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka "Braduljica", Srbija
EP  - 299
IS  - 3
SP  - 289
VL  - 35
DO  - 10.2298/BAH1903289M
ER  - 

@article{
author = "Molerović, Nikola and Rašković, Božidar and Djedović, Radica and Ostojić-Andrić, Dušica and Marković, Zoran and Marić, Saša",
year = "2019",
abstract = "Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock., U cilju zaštite ţivotne sredine i genetiĉkih resursa potoĉne pastrmke (Salmo trutta) u radu je ispitivana genetiĉka struktura ove vrste na ribnjaku ―Braduljica‖ u blizini Ivanjice. DNK je izolovana iz odseĉaka peraja 10 jedinki, a nakon toga molekularne PCR-RFLP metode su korišćene za razlikovanje izmeĊu atlantske i dunavske linije na bazi kontrolnog regiona mitohondrijalne DNK (KR mtDNK) i jedarnog gena za laktat dehidrogenazu (LDH nDNK). Na osnovu fenotipskih karakteristika procenjeno je da je pet jedinki pripadalo alohtonoj atlantskoj liniji, a preostalih pet autohtonoj dunavskoj liniji potoĉne pastrmke. MeĊutim, rezultati molekularnih analiza pokazali su prisustvo visokog procenta alohtonih gena u analiziranom uzorku, što potvrĊuje hibridizaciju izmeĊu ove dve linije. TakoĊe, rezultati ukazuju na to da selekcija na osnovu fenotipa nije adekvatna. Kako bi ribnjak nastavio da se bavi gajenjem potoĉne pastrmke za potrebe poribljavanja okolnih reka, neophodno je da se eliminišu jedinke atlantske linije iz matiĉnog fonda.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia, Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka "Braduljica", Srbija",
pages = "299-289",
number = "3",
volume = "35",
doi = "10.2298/BAH1903289M"
}

Molerović, N., Rašković, B., Djedović, R., Ostojić-Andrić, D., Marković, Z.,& Marić, S.. (2019). Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(3), 289-299.
https://doi.org/10.2298/BAH1903289M

Molerović N, Rašković B, Djedović R, Ostojić-Andrić D, Marković Z, Marić S. Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia. in Biotechnology in Animal Husbandry. 2019;35(3):289-299.
doi:10.2298/BAH1903289M .

Molerović, Nikola, Rašković, Božidar, Djedović, Radica, Ostojić-Andrić, Dušica, Marković, Zoran, Marić, Saša, "Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia" in Biotechnology in Animal Husbandry, 35, no. 3 (2019):289-299,
https://doi.org/10.2298/BAH1903289M . .