TY  - JOUR
AU  - Vranković, Jelena
AU  - Zivić, Miroslav
AU  - Radojević, Andelina
AU  - Perić-Mataruga, Vesna
AU  - Todorović, Dajana
AU  - Marković, Zoran
AU  - Živić, Ivana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4663
AB  - The current study reports a combined seasonal monitoring of the Crnica River watercourse using physicochemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs
EP  - 95
SP  - 84
VL  - 163
DO  - 10.1016/j.ecoenv.2018.07.061
ER  - 

@article{
author = "Vranković, Jelena and Zivić, Miroslav and Radojević, Andelina and Perić-Mataruga, Vesna and Todorović, Dajana and Marković, Zoran and Živić, Ivana",
year = "2018",
abstract = "The current study reports a combined seasonal monitoring of the Crnica River watercourse using physicochemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs",
pages = "95-84",
volume = "163",
doi = "10.1016/j.ecoenv.2018.07.061"
}

Vranković, J., Zivić, M., Radojević, A., Perić-Mataruga, V., Todorović, D., Marković, Z.,& Živić, I.. (2018). Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 163, 84-95.
https://doi.org/10.1016/j.ecoenv.2018.07.061

Vranković J, Zivić M, Radojević A, Perić-Mataruga V, Todorović D, Marković Z, Živić I. Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety. 2018;163:84-95.
doi:10.1016/j.ecoenv.2018.07.061 .

Vranković, Jelena, Zivić, Miroslav, Radojević, Andelina, Perić-Mataruga, Vesna, Todorović, Dajana, Marković, Zoran, Živić, Ivana, "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs" in Ecotoxicology and Environmental Safety, 163 (2018):84-95,
https://doi.org/10.1016/j.ecoenv.2018.07.061 . .

TY  - JOUR
AU  - Božanić, Milenka L.J.
AU  - Todorović, Dajana D.
AU  - Zivić, Miroslav Z.
AU  - Perić-Mataruga, Vesna
AU  - Marković, Zoran
AU  - Živić, Ivana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4713
AB  - The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1 - control, and SK2, SK3, and SK4 - 30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapez River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3-), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3-. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.
PB  - Edp Sciences S A, Les Ulis Cedex A
T2  - Knowledge and Management of Aquatic Ecosystems
T1  - Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)
IS  - 419
DO  - 10.1051/kmae/2018036
ER  - 

@article{
author = "Božanić, Milenka L.J. and Todorović, Dajana D. and Zivić, Miroslav Z. and Perić-Mataruga, Vesna and Marković, Zoran and Živić, Ivana",
year = "2018",
abstract = "The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1 - control, and SK2, SK3, and SK4 - 30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapez River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3-), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3-. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.",
publisher = "Edp Sciences S A, Les Ulis Cedex A",
journal = "Knowledge and Management of Aquatic Ecosystems",
title = "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)",
number = "419",
doi = "10.1051/kmae/2018036"
}

Božanić, M. L.J., Todorović, D. D., Zivić, M. Z., Perić-Mataruga, V., Marković, Z.,& Živić, I.. (2018). Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems
Edp Sciences S A, Les Ulis Cedex A.(419).
https://doi.org/10.1051/kmae/2018036

Božanić ML, Todorović DD, Zivić MZ, Perić-Mataruga V, Marković Z, Živić I. Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems. 2018;(419).
doi:10.1051/kmae/2018036 .

Božanić, Milenka L.J., Todorović, Dajana D., Zivić, Miroslav Z., Perić-Mataruga, Vesna, Marković, Zoran, Živić, Ivana, "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)" in Knowledge and Management of Aquatic Ecosystems, no. 419 (2018),
https://doi.org/10.1051/kmae/2018036 . .