TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5886
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI AG
T2  - Antioxidants
T1  - Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen
IS  - 7
SP  - 1091
VL  - 10
DO  - 10.3390/antiox10071091
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen",
number = "7",
pages = "1091",
volume = "10",
doi = "10.3390/antiox10071091"
}

Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants
MDPI AG., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Buechler, R.
AU  - Uzunov, A.
AU  - Kovacić, M.
AU  - Presern, J.
AU  - Pietropaoli, M.
AU  - Hatjina, F.
AU  - Pavlov, B.
AU  - Charistos, L.
AU  - Formato, G.
AU  - Galarza, E.
AU  - Gerula, D.
AU  - Gregorc, A.
AU  - Malagnini, V
AU  - Meixner, M.D.
AU  - Nedić, Nebojša
AU  - Puskadija, Z.
AU  - Rivera-Gomis, J.
AU  - Jenko, M.Rogelj
AU  - Skerl, M.I.Smodis
AU  - Vallon, J.
AU  - Vojt, Denis
AU  - Wilde, J.
AU  - Nanetti, A.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5274
AB  - Most Varroa induced colony losses occur during the autumn or winter season as a consequence of an elevated Varroa infestation level and an insufficient health status of the adult bees. Even with an initial low Varroa infestation in early spring, critical mite and virus infection levels can be reached before winter if colonies continuously rear brood throughout the whole season. To overcome this challenge, beekeepers can artificially interrupt brood production by suitable management procedures, depending on their type of beekeeping operation. To assess their efficacy, associated workload, and impact on colony development, different methods for brood interruption (queen caging with the combination of oxalic acid treatment, total brood removal, trapping comb technique) were tested during two seasons in 11 locations on 370 colonies in 10 European countries. A protocol was developed to standardize the methods' application across different environmental conditions. The efficacy of queen caging depended on the mode of oxalic acid application and ranged from 48.16% to 89.57% mite removal. The highest efficacies were achieved with trickling a 4.2% solution (89.57%) and with the sublimation of 2 g of oxalic acid (average of 88.25%) in the broodless period. The efficacy of the purely biotechnical, chemical-free trapping comb and brood removal methods did not differ significantly from the queen caging groups. We conclude that a proper application of one of the described brood interruption methods can significantly contribute to an efficient Varroa control and to the production of honey bee products meeting the highest quality and food-safety standards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Summer brood interruption as integrated management strategy for effective Varroa control in Europe
EP  - 773
IS  - 5
SP  - 764
VL  - 59
DO  - 10.1080/00218839.2020.1793278
ER  - 

@article{
author = "Buechler, R. and Uzunov, A. and Kovacić, M. and Presern, J. and Pietropaoli, M. and Hatjina, F. and Pavlov, B. and Charistos, L. and Formato, G. and Galarza, E. and Gerula, D. and Gregorc, A. and Malagnini, V and Meixner, M.D. and Nedić, Nebojša and Puskadija, Z. and Rivera-Gomis, J. and Jenko, M.Rogelj and Skerl, M.I.Smodis and Vallon, J. and Vojt, Denis and Wilde, J. and Nanetti, A.",
year = "2020",
abstract = "Most Varroa induced colony losses occur during the autumn or winter season as a consequence of an elevated Varroa infestation level and an insufficient health status of the adult bees. Even with an initial low Varroa infestation in early spring, critical mite and virus infection levels can be reached before winter if colonies continuously rear brood throughout the whole season. To overcome this challenge, beekeepers can artificially interrupt brood production by suitable management procedures, depending on their type of beekeeping operation. To assess their efficacy, associated workload, and impact on colony development, different methods for brood interruption (queen caging with the combination of oxalic acid treatment, total brood removal, trapping comb technique) were tested during two seasons in 11 locations on 370 colonies in 10 European countries. A protocol was developed to standardize the methods' application across different environmental conditions. The efficacy of queen caging depended on the mode of oxalic acid application and ranged from 48.16% to 89.57% mite removal. The highest efficacies were achieved with trickling a 4.2% solution (89.57%) and with the sublimation of 2 g of oxalic acid (average of 88.25%) in the broodless period. The efficacy of the purely biotechnical, chemical-free trapping comb and brood removal methods did not differ significantly from the queen caging groups. We conclude that a proper application of one of the described brood interruption methods can significantly contribute to an efficient Varroa control and to the production of honey bee products meeting the highest quality and food-safety standards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Summer brood interruption as integrated management strategy for effective Varroa control in Europe",
pages = "773-764",
number = "5",
volume = "59",
doi = "10.1080/00218839.2020.1793278"
}

Buechler, R., Uzunov, A., Kovacić, M., Presern, J., Pietropaoli, M., Hatjina, F., Pavlov, B., Charistos, L., Formato, G., Galarza, E., Gerula, D., Gregorc, A., Malagnini, V., Meixner, M.D., Nedić, N., Puskadija, Z., Rivera-Gomis, J., Jenko, M.Rogelj, Skerl, M.I.Smodis, Vallon, J., Vojt, D., Wilde, J.,& Nanetti, A.. (2020). Summer brood interruption as integrated management strategy for effective Varroa control in Europe. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 59(5), 764-773.
https://doi.org/10.1080/00218839.2020.1793278

Buechler R, Uzunov A, Kovacić M, Presern J, Pietropaoli M, Hatjina F, Pavlov B, Charistos L, Formato G, Galarza E, Gerula D, Gregorc A, Malagnini V, Meixner M, Nedić N, Puskadija Z, Rivera-Gomis J, Jenko M, Skerl M, Vallon J, Vojt D, Wilde J, Nanetti A. Summer brood interruption as integrated management strategy for effective Varroa control in Europe. in Journal of Apicultural Research. 2020;59(5):764-773.
doi:10.1080/00218839.2020.1793278 .

Buechler, R., Uzunov, A., Kovacić, M., Presern, J., Pietropaoli, M., Hatjina, F., Pavlov, B., Charistos, L., Formato, G., Galarza, E., Gerula, D., Gregorc, A., Malagnini, V, Meixner, M.D., Nedić, Nebojša, Puskadija, Z., Rivera-Gomis, J., Jenko, M.Rogelj, Skerl, M.I.Smodis, Vallon, J., Vojt, Denis, Wilde, J., Nanetti, A., "Summer brood interruption as integrated management strategy for effective Varroa control in Europe" in Journal of Apicultural Research, 59, no. 5 (2020):764-773,
https://doi.org/10.1080/00218839.2020.1793278 . .

TY  - JOUR
AU  - Jovetić, Milica S.
AU  - Redzepović, Azra S.
AU  - Nedić, Nebojša
AU  - Vojt, Denis
AU  - Durdić, Sladana Z.
AU  - Brčeski, Ilija
AU  - Milojković-Opsenica, Dušanka M.
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4627
AB  - To contribute to the development of urban beekeeping, we designed this study to obtain more information about the contamination of urban bee products with toxic metals, polycyclic aromatic hydrocarbons (PAHs), and pesticides. The samples of honey (N=23), pollen (N=13), and floral nectar (N=6) were collected from the experimental stationary apiary of the Belgrade University Faculty of Agriculture located in centre of Zemun (a municipality of the Belgrade metropolitan area) in 2015 and 2016. Metals (Pb, Cd, As, Cu, Zn, Fe, Mn, Ni, Cr, and Hg) were determined with inductively coupled plasma quadrupole mass spectrometry (ICP-QMS). Polycyclic aromatic hydrocarbons (PAHs) were analysed with high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Pesticides were analysed with gas chromatography - mass spectrometry (GC-MS). The honey samples were generally within the European and Serbian regulatory limits. The levels of all the 123 analysed pesticides were below the limit of quantification (LOQ). Regarding PAH levels in honey, the highest content was found for naphthalene. The elevated levels of Hg and Cr and of PAHs in the pollen samples indicated air pollution. Pesticide residues in pollen, however, were below the LOQ. In nectar, metal levels were relatively similar to those in honey. Our results suggest that the investigated urban honey meets the regulatory requirements for metals, PAHs, and pesticides and is therefore safe for consumption.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Urban honey - the aspects of its safety
EP  - 274
IS  - 3
SP  - 264
VL  - 69
DO  - 10.2478/aiht-2018-69-3126
ER  - 

@article{
author = "Jovetić, Milica S. and Redzepović, Azra S. and Nedić, Nebojša and Vojt, Denis and Durdić, Sladana Z. and Brčeski, Ilija and Milojković-Opsenica, Dušanka M.",
year = "2018",
abstract = "To contribute to the development of urban beekeeping, we designed this study to obtain more information about the contamination of urban bee products with toxic metals, polycyclic aromatic hydrocarbons (PAHs), and pesticides. The samples of honey (N=23), pollen (N=13), and floral nectar (N=6) were collected from the experimental stationary apiary of the Belgrade University Faculty of Agriculture located in centre of Zemun (a municipality of the Belgrade metropolitan area) in 2015 and 2016. Metals (Pb, Cd, As, Cu, Zn, Fe, Mn, Ni, Cr, and Hg) were determined with inductively coupled plasma quadrupole mass spectrometry (ICP-QMS). Polycyclic aromatic hydrocarbons (PAHs) were analysed with high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Pesticides were analysed with gas chromatography - mass spectrometry (GC-MS). The honey samples were generally within the European and Serbian regulatory limits. The levels of all the 123 analysed pesticides were below the limit of quantification (LOQ). Regarding PAH levels in honey, the highest content was found for naphthalene. The elevated levels of Hg and Cr and of PAHs in the pollen samples indicated air pollution. Pesticide residues in pollen, however, were below the LOQ. In nectar, metal levels were relatively similar to those in honey. Our results suggest that the investigated urban honey meets the regulatory requirements for metals, PAHs, and pesticides and is therefore safe for consumption.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Urban honey - the aspects of its safety",
pages = "274-264",
number = "3",
volume = "69",
doi = "10.2478/aiht-2018-69-3126"
}

Jovetić, M. S., Redzepović, A. S., Nedić, N., Vojt, D., Durdić, S. Z., Brčeski, I.,& Milojković-Opsenica, D. M.. (2018). Urban honey - the aspects of its safety. in Arhiv za higijenu rada i toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(3), 264-274.
https://doi.org/10.2478/aiht-2018-69-3126

Jovetić MS, Redzepović AS, Nedić N, Vojt D, Durdić SZ, Brčeski I, Milojković-Opsenica DM. Urban honey - the aspects of its safety. in Arhiv za higijenu rada i toksikologiju-Archives of Industrial Hygiene and Toxicology. 2018;69(3):264-274.
doi:10.2478/aiht-2018-69-3126 .

Jovetić, Milica S., Redzepović, Azra S., Nedić, Nebojša, Vojt, Denis, Durdić, Sladana Z., Brčeski, Ilija, Milojković-Opsenica, Dušanka M., "Urban honey - the aspects of its safety" in Arhiv za higijenu rada i toksikologiju-Archives of Industrial Hygiene and Toxicology, 69, no. 3 (2018):264-274,
https://doi.org/10.2478/aiht-2018-69-3126 . .