TY  - JOUR
AU  - Delahaut, Vyshal
AU  - Rašković, Božidar
AU  - Salvado, Marta Satorres
AU  - Bervoets, Lieven
AU  - Blust, Ronny
AU  - De Boeck, Gudrun
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5377
AB  - The individual toxicity and bioaccumulation of cadmium, copper and zinc for common carp juveniles was evaluated in a direct comparison in two experimental setups. First, fish were exposed for 10 days to different metal concentrations in order to link metal bioaccumulation to LC50 values (concentration lethal to 50% of the animals) and incipient lethal levels (ILL, concentration where 50% survives indefinitely). Accumulated metals showed a positive dose dependent uptake for cadmium and copper, but not for zinc. Toxicity was in the order cadmium>copper>zinc with 96h LC50 values for cadmium at 0.20 +/- 0.16 mu M, for copper at 0.77 +/- 0.03 mu M, and for zinc at 29.89 +/- 9.03 mu M respectively. For copper, the 96h exposure was sufficient to calculate the incipient lethal level and therefore 96h LC50 and ILL levels were the same, while for cadmium and zinc 5 to 6 days were needed to reach ILL resulting in slightly lower values at 0.16 mu M and 28.33 mu M respectively. Subsequently, a subacute exposure experiment was conducted, where carp juveniles were exposed to 2 equitoxic concentrations (10% and 50% of LC50 96 h) of the three metals for 1, 3 and 7 days. Again a significant dose-dependent increase in gill cadmium and copper, but not in zinc, was observed during the 7-day exposure. Copper clearly affected sodium levels in gill tissue, while zinc and cadmium did not significantly alter any of the gill electrolytes. The overall histopathological effects (e.g. hyperemia and hypertrophy) of the metal exposures were mild for most of the alterations. Our study showed that copper an cadmium (but not zinc) showed dose dependent metal accumulation, however this bioaccumulation was only correlated with mortality for cadmium. Metal specific alterations were reduced gill sodium levels in copper exposed fish and oedema of the primary epithelium which typically occurred in both levels of zinc exposure.
PB  - Public Library Science, San Francisco
T2  - Plos One
T1  - Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles
IS  - 4
VL  - 15
DO  - 10.1371/journal.pone.0220485
ER  - 

@article{
author = "Delahaut, Vyshal and Rašković, Božidar and Salvado, Marta Satorres and Bervoets, Lieven and Blust, Ronny and De Boeck, Gudrun",
year = "2020",
abstract = "The individual toxicity and bioaccumulation of cadmium, copper and zinc for common carp juveniles was evaluated in a direct comparison in two experimental setups. First, fish were exposed for 10 days to different metal concentrations in order to link metal bioaccumulation to LC50 values (concentration lethal to 50% of the animals) and incipient lethal levels (ILL, concentration where 50% survives indefinitely). Accumulated metals showed a positive dose dependent uptake for cadmium and copper, but not for zinc. Toxicity was in the order cadmium>copper>zinc with 96h LC50 values for cadmium at 0.20 +/- 0.16 mu M, for copper at 0.77 +/- 0.03 mu M, and for zinc at 29.89 +/- 9.03 mu M respectively. For copper, the 96h exposure was sufficient to calculate the incipient lethal level and therefore 96h LC50 and ILL levels were the same, while for cadmium and zinc 5 to 6 days were needed to reach ILL resulting in slightly lower values at 0.16 mu M and 28.33 mu M respectively. Subsequently, a subacute exposure experiment was conducted, where carp juveniles were exposed to 2 equitoxic concentrations (10% and 50% of LC50 96 h) of the three metals for 1, 3 and 7 days. Again a significant dose-dependent increase in gill cadmium and copper, but not in zinc, was observed during the 7-day exposure. Copper clearly affected sodium levels in gill tissue, while zinc and cadmium did not significantly alter any of the gill electrolytes. The overall histopathological effects (e.g. hyperemia and hypertrophy) of the metal exposures were mild for most of the alterations. Our study showed that copper an cadmium (but not zinc) showed dose dependent metal accumulation, however this bioaccumulation was only correlated with mortality for cadmium. Metal specific alterations were reduced gill sodium levels in copper exposed fish and oedema of the primary epithelium which typically occurred in both levels of zinc exposure.",
publisher = "Public Library Science, San Francisco",
journal = "Plos One",
title = "Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles",
number = "4",
volume = "15",
doi = "10.1371/journal.pone.0220485"
}

Delahaut, V., Rašković, B., Salvado, M. S., Bervoets, L., Blust, R.,& De Boeck, G.. (2020). Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles. in Plos One
Public Library Science, San Francisco., 15(4).
https://doi.org/10.1371/journal.pone.0220485

Delahaut V, Rašković B, Salvado MS, Bervoets L, Blust R, De Boeck G. Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles. in Plos One. 2020;15(4).
doi:10.1371/journal.pone.0220485 .

Delahaut, Vyshal, Rašković, Božidar, Salvado, Marta Satorres, Bervoets, Lieven, Blust, Ronny, De Boeck, Gudrun, "Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles" in Plos One, 15, no. 4 (2020),
https://doi.org/10.1371/journal.pone.0220485 . .

TY  - JOUR
AU  - Shrivastava, Jyotsna
AU  - Rašković, Božidar
AU  - Blust, Ronny
AU  - De Boeck, Gudrun
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4643
AB  - It has been suggested that induced swimming has the potential to improve the growth performance of fish. We tested this hypothesis by measuring growth, metabolic efficiency and physiological capacity of common carp (Cyprinus carpio). Fish were swum at different exercise regimes: 0.0 (control), 1.5 and 2.5 body lengths per second (BL/s) in 1600 L recirculating raceways for 4 weeks. The results showed a significant increase in weight gain, specific growth rate, improved feed conversion efficiency, and a higher hepatosomatic index for 2.5 BL/s exercised fish compared to control. Glycogen, protein and lipid energy stores in hepatic and muscular tissue showed limited differences among experimental groups. Likewise, plasma [Na+], [K+] and [Cl- ] remained stable at all swimming regimes. Expression of genes controlling energy metabolism and growth (IGF-I axis, cytochrome oxidase) and stress response (cortisol receptor, heat shock protein 70) revealed clear regulatory roles as the mRNA transcript levels of IGF-I and growth hormone receptors in hepatic tissue were up-regulated in fish exercised for 3-4 weeks at 2.5 BL/s. Oxygen consumption rate and swimming performance (U-crit) for each experimental group were evaluated in parallel in Blazka-type swim-tunnels (3.9 L) and showed no training effect while prolonged swimming at 1.5 and 2.5 BL/s facilitated ammonia excretion and prevented build-up of plasma ammonia. Overall, these data suggest that sustained exercise at 2.5 BL/s enhanced growth and physiological fitness without compromising energy metabolism or ion-regulation. Our study provides a prospective of implementing exercise as a tool to increase fish production efficiency in commercial aquaculture systems.
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
T1  - Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)
EP  - 48
SP  - 38
VL  - 226
DO  - 10.1016/j.cbpa.2018.08.007
ER  - 

@article{
author = "Shrivastava, Jyotsna and Rašković, Božidar and Blust, Ronny and De Boeck, Gudrun",
year = "2018",
abstract = "It has been suggested that induced swimming has the potential to improve the growth performance of fish. We tested this hypothesis by measuring growth, metabolic efficiency and physiological capacity of common carp (Cyprinus carpio). Fish were swum at different exercise regimes: 0.0 (control), 1.5 and 2.5 body lengths per second (BL/s) in 1600 L recirculating raceways for 4 weeks. The results showed a significant increase in weight gain, specific growth rate, improved feed conversion efficiency, and a higher hepatosomatic index for 2.5 BL/s exercised fish compared to control. Glycogen, protein and lipid energy stores in hepatic and muscular tissue showed limited differences among experimental groups. Likewise, plasma [Na+], [K+] and [Cl- ] remained stable at all swimming regimes. Expression of genes controlling energy metabolism and growth (IGF-I axis, cytochrome oxidase) and stress response (cortisol receptor, heat shock protein 70) revealed clear regulatory roles as the mRNA transcript levels of IGF-I and growth hormone receptors in hepatic tissue were up-regulated in fish exercised for 3-4 weeks at 2.5 BL/s. Oxygen consumption rate and swimming performance (U-crit) for each experimental group were evaluated in parallel in Blazka-type swim-tunnels (3.9 L) and showed no training effect while prolonged swimming at 1.5 and 2.5 BL/s facilitated ammonia excretion and prevented build-up of plasma ammonia. Overall, these data suggest that sustained exercise at 2.5 BL/s enhanced growth and physiological fitness without compromising energy metabolism or ion-regulation. Our study provides a prospective of implementing exercise as a tool to increase fish production efficiency in commercial aquaculture systems.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology",
title = "Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)",
pages = "48-38",
volume = "226",
doi = "10.1016/j.cbpa.2018.08.007"
}

Shrivastava, J., Rašković, B., Blust, R.,& De Boeck, G.. (2018). Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio). in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
Elsevier Science Inc, New York., 226, 38-48.
https://doi.org/10.1016/j.cbpa.2018.08.007

Shrivastava J, Rašković B, Blust R, De Boeck G. Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio). in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. 2018;226:38-48.
doi:10.1016/j.cbpa.2018.08.007 .

Shrivastava, Jyotsna, Rašković, Božidar, Blust, Ronny, De Boeck, Gudrun, "Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)" in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 226 (2018):38-48,
https://doi.org/10.1016/j.cbpa.2018.08.007 . .