TY  - JOUR
AU  - Beatović, Damir
AU  - Krstić-Milošević, Dijana
AU  - Trifunović, Snežana
AU  - Siljegović, Jovana
AU  - Glamoclija, Jasmina
AU  - Ristić, Mihailo
AU  - Jelačić, Slavica
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3791
AB  - This study evaluated the chemical composition, antioxidant and antimicrobial activities of the essential oils of twelve Ocimum basilicum L. cultivars grown in Serbia. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The oil yields ranged from 0.65 to 1.90 %. A total of 75 compounds were identified as constituents of analyzed essential oils. GC/MS analyses revealed that a majority of the examined basil cultivars belonged to the "linalool chemotype". The results of the DPPH assay showed a very high antioxidant capacity of the basil oils, which was especially high for Blue Spice cultivar (IC50 = 0.03 mu g/mL). Significant antimicrobial activity was shown for all the tested oils. Essential oil of Compact cultivar was the most active against the bacterium Micrococcus flavus, with an MIC value of 0.009 mu g/mL while Osmin cultivar exhibited the strongest antifungal activity with MIC values ranging from 0.08-1.07 mu g/mL. All the examined essential oils showed a 10-to 100-fold greater ability to inhibit fungal growth compared to commercial antifungal agents.
PB  - ACG Publications, Gebze-Kocaeli
T2  - Records of Natural Products
T1  - Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia
EP  - 75
IS  - 1
SP  - 62
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1672
ER  - 

@article{
author = "Beatović, Damir and Krstić-Milošević, Dijana and Trifunović, Snežana and Siljegović, Jovana and Glamoclija, Jasmina and Ristić, Mihailo and Jelačić, Slavica",
year = "2015",
abstract = "This study evaluated the chemical composition, antioxidant and antimicrobial activities of the essential oils of twelve Ocimum basilicum L. cultivars grown in Serbia. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The oil yields ranged from 0.65 to 1.90 %. A total of 75 compounds were identified as constituents of analyzed essential oils. GC/MS analyses revealed that a majority of the examined basil cultivars belonged to the "linalool chemotype". The results of the DPPH assay showed a very high antioxidant capacity of the basil oils, which was especially high for Blue Spice cultivar (IC50 = 0.03 mu g/mL). Significant antimicrobial activity was shown for all the tested oils. Essential oil of Compact cultivar was the most active against the bacterium Micrococcus flavus, with an MIC value of 0.009 mu g/mL while Osmin cultivar exhibited the strongest antifungal activity with MIC values ranging from 0.08-1.07 mu g/mL. All the examined essential oils showed a 10-to 100-fold greater ability to inhibit fungal growth compared to commercial antifungal agents.",
publisher = "ACG Publications, Gebze-Kocaeli",
journal = "Records of Natural Products",
title = "Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia",
pages = "75-62",
number = "1",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1672"
}

Beatović, D., Krstić-Milošević, D., Trifunović, S., Siljegović, J., Glamoclija, J., Ristić, M.,& Jelačić, S.. (2015). Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia. in Records of Natural Products
ACG Publications, Gebze-Kocaeli., 9(1), 62-75.
https://hdl.handle.net/21.15107/rcub_cherry_1672

Beatović D, Krstić-Milošević D, Trifunović S, Siljegović J, Glamoclija J, Ristić M, Jelačić S. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia. in Records of Natural Products. 2015;9(1):62-75.
https://hdl.handle.net/21.15107/rcub_cherry_1672 .

Beatović, Damir, Krstić-Milošević, Dijana, Trifunović, Snežana, Siljegović, Jovana, Glamoclija, Jasmina, Ristić, Mihailo, Jelačić, Slavica, "Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia" in Records of Natural Products, 9, no. 1 (2015):62-75,
https://hdl.handle.net/21.15107/rcub_cherry_1672 .

TY  - JOUR
AU  - Pljevljakusić, Dejan
AU  - Rančić, Dragana
AU  - Ristić, Mihailo
AU  - Vujisić, Ljubodrag
AU  - Radanović, Dragoja
AU  - Dajić-Stevanović, Zora
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3039
AB  - Arnica montana (L) is a medicinal plant whose flower heads are widely used for the topical treatment of bruises and sprains in phytopharmaceutical preparations. Despite being cultivated for flower heads production for a long time, the quantity and quality of its underground parts have not received much attention. In this study underground parts of a three-year cultivation trial have been estimated for yield potential and chemical composition of essential oil in two consecutive years. Yields of rhizome dry mass were about 208 kg ha(-1) and 647 kg ha(-1), and root dry mass were about 330 kg ha(-1) and 525 kg ha(-1) in the second and third year of growing period, respectively. The chemical composition of the essential oil from root and rhizome, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. At the end of second year essential oils from rhizome and root, yielded on average about 4.05% and 1.89%, and at the end of third year, they yielded 2.43% and 1.85%, respectively. The composition of essential oils was independent regarding cultivation trial factors with small differences regarding years. Aromatic hydrocarbons were predominant group of essential oil constituents (about 84% in average) and were identified as thymol derivates. Main individual components were 2,5-dimethoxy-p-cymene, thymol methyl ether and 2,6-diisopropylanisole. Furthermore, histochemical localization of essential oils in rhizome and root was carried out. Secretory structures responsible for essential oil synthesis and accumulation were studied using light, scanning and transmission microscopy. Secretory tissue is localized in the cortical region of the root and rhizome in form of idioblastic secretory cells and secretory cavities, respectively. Histochemical analyses confirmed the lipid nature of secreted material.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil
EP  - 189
SP  - 177
VL  - 39
DO  - 10.1016/j.indcrop.2012.02.030
ER  - 

@article{
author = "Pljevljakusić, Dejan and Rančić, Dragana and Ristić, Mihailo and Vujisić, Ljubodrag and Radanović, Dragoja and Dajić-Stevanović, Zora",
year = "2012",
abstract = "Arnica montana (L) is a medicinal plant whose flower heads are widely used for the topical treatment of bruises and sprains in phytopharmaceutical preparations. Despite being cultivated for flower heads production for a long time, the quantity and quality of its underground parts have not received much attention. In this study underground parts of a three-year cultivation trial have been estimated for yield potential and chemical composition of essential oil in two consecutive years. Yields of rhizome dry mass were about 208 kg ha(-1) and 647 kg ha(-1), and root dry mass were about 330 kg ha(-1) and 525 kg ha(-1) in the second and third year of growing period, respectively. The chemical composition of the essential oil from root and rhizome, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. At the end of second year essential oils from rhizome and root, yielded on average about 4.05% and 1.89%, and at the end of third year, they yielded 2.43% and 1.85%, respectively. The composition of essential oils was independent regarding cultivation trial factors with small differences regarding years. Aromatic hydrocarbons were predominant group of essential oil constituents (about 84% in average) and were identified as thymol derivates. Main individual components were 2,5-dimethoxy-p-cymene, thymol methyl ether and 2,6-diisopropylanisole. Furthermore, histochemical localization of essential oils in rhizome and root was carried out. Secretory structures responsible for essential oil synthesis and accumulation were studied using light, scanning and transmission microscopy. Secretory tissue is localized in the cortical region of the root and rhizome in form of idioblastic secretory cells and secretory cavities, respectively. Histochemical analyses confirmed the lipid nature of secreted material.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil",
pages = "189-177",
volume = "39",
doi = "10.1016/j.indcrop.2012.02.030"
}

Pljevljakusić, D., Rančić, D., Ristić, M., Vujisić, L., Radanović, D.,& Dajić-Stevanović, Z.. (2012). Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil. in Industrial Crops and Products
Elsevier, Amsterdam., 39, 177-189.
https://doi.org/10.1016/j.indcrop.2012.02.030

Pljevljakusić D, Rančić D, Ristić M, Vujisić L, Radanović D, Dajić-Stevanović Z. Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil. in Industrial Crops and Products. 2012;39:177-189.
doi:10.1016/j.indcrop.2012.02.030 .

Pljevljakusić, Dejan, Rančić, Dragana, Ristić, Mihailo, Vujisić, Ljubodrag, Radanović, Dragoja, Dajić-Stevanović, Zora, "Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil" in Industrial Crops and Products, 39 (2012):177-189,
https://doi.org/10.1016/j.indcrop.2012.02.030 . .

TY  - JOUR
AU  - Jug-Dujaković, Marija
AU  - Ristić, Mihailo
AU  - Pljevljakusić, Dejan
AU  - Dajić-Stevanović, Zora
AU  - Liber, Zlatko
AU  - Hancević, Katarina
AU  - Radić, Tomislav
AU  - Satović, Zlatko
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2976
AB  - Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition
EP  - 2323
IS  - 10
SP  - 2309
VL  - 9
DO  - 10.1002/cbdv.201200131
ER  - 

@article{
author = "Jug-Dujaković, Marija and Ristić, Mihailo and Pljevljakusić, Dejan and Dajić-Stevanović, Zora and Liber, Zlatko and Hancević, Katarina and Radić, Tomislav and Satović, Zlatko",
year = "2012",
abstract = "Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition",
pages = "2323-2309",
number = "10",
volume = "9",
doi = "10.1002/cbdv.201200131"
}

Jug-Dujaković, M., Ristić, M., Pljevljakusić, D., Dajić-Stevanović, Z., Liber, Z., Hancević, K., Radić, T.,& Satović, Z.. (2012). High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 9(10), 2309-2323.
https://doi.org/10.1002/cbdv.201200131

Jug-Dujaković M, Ristić M, Pljevljakusić D, Dajić-Stevanović Z, Liber Z, Hancević K, Radić T, Satović Z. High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity. 2012;9(10):2309-2323.
doi:10.1002/cbdv.201200131 .

Jug-Dujaković, Marija, Ristić, Mihailo, Pljevljakusić, Dejan, Dajić-Stevanović, Zora, Liber, Zlatko, Hancević, Katarina, Radić, Tomislav, Satović, Zlatko, "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition" in Chemistry & Biodiversity, 9, no. 10 (2012):2309-2323,
https://doi.org/10.1002/cbdv.201200131 . .

TY  - JOUR
AU  - Ibraliu, Alban
AU  - Mi, X.
AU  - Ristić, Mihailo
AU  - Dajić-Stevanović, Zora
AU  - Shehu, J.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2410
AB  - Seventeen populations of Orignanum vulgare (four populations), Thymus capitatus (four populations) and Satureja montana (Origanum) were collected from different agro-climatically diverse sites in Albania. Their essential oils were subjected to detailed GC/FID and GC/MS analyses to determine similarities and differences among them for their chemical composition, especially the major compounds, such as Carvacrol and Thymol, which carry economic importance in medicinal plant industry and food industry. The concentrations of Carvacrol varied from 21.07 to 77.79%; Thymol from 0.72 to 39.9%; y-Terpinene from 4 to 13.8% and p-Cymene from 0.74 to 17.4%. From the cluster analysis (CA) accomplished by using the statistical software R, we came to two conclusions. (1) The collections of O. vulgare subsp. hirtum (rich in Carvacrol) and Th. capitatus (balanced concentration of compound) made from identical locations belonged to two different sub-groups, whereas the populations of S. montana were divided into these two groups based on their oil compositions. (2) There were no appreciable environmental effects on oil concentration.
T2  - Journal of Medicinal Plants Research
T1  - Analysis of essential oils of three wild medicinal plants in Albania
EP  - 62
IS  - 1
SP  - 58
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2410
ER  - 

@article{
author = "Ibraliu, Alban and Mi, X. and Ristić, Mihailo and Dajić-Stevanović, Zora and Shehu, J.",
year = "2011",
abstract = "Seventeen populations of Orignanum vulgare (four populations), Thymus capitatus (four populations) and Satureja montana (Origanum) were collected from different agro-climatically diverse sites in Albania. Their essential oils were subjected to detailed GC/FID and GC/MS analyses to determine similarities and differences among them for their chemical composition, especially the major compounds, such as Carvacrol and Thymol, which carry economic importance in medicinal plant industry and food industry. The concentrations of Carvacrol varied from 21.07 to 77.79%; Thymol from 0.72 to 39.9%; y-Terpinene from 4 to 13.8% and p-Cymene from 0.74 to 17.4%. From the cluster analysis (CA) accomplished by using the statistical software R, we came to two conclusions. (1) The collections of O. vulgare subsp. hirtum (rich in Carvacrol) and Th. capitatus (balanced concentration of compound) made from identical locations belonged to two different sub-groups, whereas the populations of S. montana were divided into these two groups based on their oil compositions. (2) There were no appreciable environmental effects on oil concentration.",
journal = "Journal of Medicinal Plants Research",
title = "Analysis of essential oils of three wild medicinal plants in Albania",
pages = "62-58",
number = "1",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2410"
}

Ibraliu, A., Mi, X., Ristić, M., Dajić-Stevanović, Z.,& Shehu, J.. (2011). Analysis of essential oils of three wild medicinal plants in Albania. in Journal of Medicinal Plants Research, 5(1), 58-62.
https://hdl.handle.net/21.15107/rcub_agrospace_2410

Ibraliu A, Mi X, Ristić M, Dajić-Stevanović Z, Shehu J. Analysis of essential oils of three wild medicinal plants in Albania. in Journal of Medicinal Plants Research. 2011;5(1):58-62.
https://hdl.handle.net/21.15107/rcub_agrospace_2410 .

Ibraliu, Alban, Mi, X., Ristić, Mihailo, Dajić-Stevanović, Zora, Shehu, J., "Analysis of essential oils of three wild medicinal plants in Albania" in Journal of Medicinal Plants Research, 5, no. 1 (2011):58-62,
https://hdl.handle.net/21.15107/rcub_agrospace_2410 .

TY  - JOUR
AU  - Maksimović, Zoran
AU  - Stojanović, Danilo
AU  - Šoštarić, Ivan
AU  - Dajić, Zora
AU  - Ristić, Mihailo
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1785
AB  - BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil
EP  - 2041
IS  - 11
SP  - 2036
VL  - 88
DO  - 10.1002/jsfa.3311
ER  - 

@article{
author = "Maksimović, Zoran and Stojanović, Danilo and Šoštarić, Ivan and Dajić, Zora and Ristić, Mihailo",
year = "2008",
abstract = "BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil",
pages = "2041-2036",
number = "11",
volume = "88",
doi = "10.1002/jsfa.3311"
}

Maksimović, Z., Stojanović, D., Šoštarić, I., Dajić, Z.,& Ristić, M.. (2008). Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 88(11), 2036-2041.
https://doi.org/10.1002/jsfa.3311

Maksimović Z, Stojanović D, Šoštarić I, Dajić Z, Ristić M. Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture. 2008;88(11):2036-2041.
doi:10.1002/jsfa.3311 .

Maksimović, Zoran, Stojanović, Danilo, Šoštarić, Ivan, Dajić, Zora, Ristić, Mihailo, "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil" in Journal of the Science of Food and Agriculture, 88, no. 11 (2008):2036-2041,
https://doi.org/10.1002/jsfa.3311 . .