TY  - JOUR
AU  - Vukosavljević, Predrag
AU  - Novaković, Miroslav
AU  - Bukvić, Branka
AU  - Nikšić, Miomir
AU  - Stanisavljević, Ivana
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1975
AB  - Bitter herbal liquors are products often consumed, not only because of their refreshment properties, but also healthy reasons. The main components of bitters are different herbal extracts which contain a lot of different bioactive compounds, such as phenols, polyphenols and their polymers, phenol carboxylic acids, organic acids flavonoids, tannins etc. At the market different products labeled as bitter herbal liquors can be found. They are made from 28, 32 or even 54 and 56 extracts obtained by alcohol or water extraction from different parts of herbs, fruits, seeds, spices, roots, etc. By definition 'bitters' are natural products made of fruits and herbs extracts permitted for human nutrition. Those products are not only alcohol beverages, but also the drinks with potentially healthful effects. Consumed in moderate ways they may provide intake of useful bioactive compounds. The paper presents extraction procedures of 46 different healthful and aromatic herbs and 8 fruits, used for production of 'Bitter 54'. Anti-oxidative activity of plant extracts was determinate by DPPH test using method of Blois. The free radical-scavenging activity of the plant extracts was evaluated using the stable radical DPPH. Synthetic antioxidants BHT (ditertbytil-hydroxytoluene, EC50= 6.2μgml-1), trolox (vitamine E analog soluble in water, EC50= 6.8μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50%. It was found that the EC50 value of Bitter kept in the green bottle in the dark has been 139.47μl/ml of solvent and it has been stable during 120 days. The results indicate that 'Bitter 54' possess significant antioxidant capacity. .
AB  - U radu su prikazani načini ekstrakcije 46 lekovitih i aromatičnih bilja i osam voćnih vrsta koji ulaze u sastav Bittera 54. Utvrđeno je da gorki biljni liker - Bitter 54 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je testom DPPH, metodom po Bloisu. Vrednost EC50 uzorka Bittera 54 koji je stajao u zatamnjenoj boci u mraku bila je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 6,2 i 6,8 μg/ml, respektivno. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju radikala DPPH. .
PB  - Društvo za ishranu Srbije, Beograd
T2  - Hrana i ishrana
T1  - Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'
T1  - Potencijalni antioksidativni kapacitet kompozicije lekovitih i aromatičnih biljaka i voća u Bitteru 54
EP  - 8
IS  - 1-2
SP  - 3
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1975
ER  - 

@article{
author = "Vukosavljević, Predrag and Novaković, Miroslav and Bukvić, Branka and Nikšić, Miomir and Stanisavljević, Ivana",
year = "2009",
abstract = "Bitter herbal liquors are products often consumed, not only because of their refreshment properties, but also healthy reasons. The main components of bitters are different herbal extracts which contain a lot of different bioactive compounds, such as phenols, polyphenols and their polymers, phenol carboxylic acids, organic acids flavonoids, tannins etc. At the market different products labeled as bitter herbal liquors can be found. They are made from 28, 32 or even 54 and 56 extracts obtained by alcohol or water extraction from different parts of herbs, fruits, seeds, spices, roots, etc. By definition 'bitters' are natural products made of fruits and herbs extracts permitted for human nutrition. Those products are not only alcohol beverages, but also the drinks with potentially healthful effects. Consumed in moderate ways they may provide intake of useful bioactive compounds. The paper presents extraction procedures of 46 different healthful and aromatic herbs and 8 fruits, used for production of 'Bitter 54'. Anti-oxidative activity of plant extracts was determinate by DPPH test using method of Blois. The free radical-scavenging activity of the plant extracts was evaluated using the stable radical DPPH. Synthetic antioxidants BHT (ditertbytil-hydroxytoluene, EC50= 6.2μgml-1), trolox (vitamine E analog soluble in water, EC50= 6.8μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50%. It was found that the EC50 value of Bitter kept in the green bottle in the dark has been 139.47μl/ml of solvent and it has been stable during 120 days. The results indicate that 'Bitter 54' possess significant antioxidant capacity. ., U radu su prikazani načini ekstrakcije 46 lekovitih i aromatičnih bilja i osam voćnih vrsta koji ulaze u sastav Bittera 54. Utvrđeno je da gorki biljni liker - Bitter 54 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je testom DPPH, metodom po Bloisu. Vrednost EC50 uzorka Bittera 54 koji je stajao u zatamnjenoj boci u mraku bila je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 6,2 i 6,8 μg/ml, respektivno. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju radikala DPPH. .",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54', Potencijalni antioksidativni kapacitet kompozicije lekovitih i aromatičnih biljaka i voća u Bitteru 54",
pages = "8-3",
number = "1-2",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1975"
}

Vukosavljević, P., Novaković, M., Bukvić, B., Nikšić, M.,& Stanisavljević, I.. (2009). Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'. in Hrana i ishrana
Društvo za ishranu Srbije, Beograd., 50(1-2), 3-8.
https://hdl.handle.net/21.15107/rcub_agrospace_1975

Vukosavljević P, Novaković M, Bukvić B, Nikšić M, Stanisavljević I. Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'. in Hrana i ishrana. 2009;50(1-2):3-8.
https://hdl.handle.net/21.15107/rcub_agrospace_1975 .

Vukosavljević, Predrag, Novaković, Miroslav, Bukvić, Branka, Nikšić, Miomir, Stanisavljević, Ivana, "Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'" in Hrana i ishrana, 50, no. 1-2 (2009):3-8,
https://hdl.handle.net/21.15107/rcub_agrospace_1975 .

TY  - JOUR
AU  - Vukosavljević, Predrag
AU  - Novaković, Miroslav
AU  - Bukvić, Branka
AU  - Nikšić, Miomir
AU  - Stanisavljević, Ivana
AU  - Klaus, Anita
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1893
AB  - This paper presents kinds of extraction and cross-flow filtration of composition of 46 healthful and aromatic herbs, 8 fruits and fungi Ganoderma lucidum. Those extracts are part of Bitter 55, which have significant antioxidant capacity. Antioxidative activities of plant extracts have been determined by DPPH test using method of Blois. Bitter 55 which was kept at the green bottle in the dark has EC50 = 141.07 μl/ml and it was stable during 150 days. Synthetic anti-oxidants BHT (ditertbutilhydroxytoluen, EC50 = 6.2 μgml-1), trolox (vitamin E analog soluble in water, EC50 = 6.8 μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50 %. Bitter 55 contents 35% vol of alcohol (wheat origin), 88.22 g/l total extract and slice of medicinal mushroom Ganoderma lucidum (1 % w/v) which was extracted 30 days before analyzes. The main problem in practical applications of MF is the reduction of permeate flux with time, caused by the accumulation of feed components in the pores. During microfiltration bitter herbal liquor, the function of filtrate flux is decreased with VCR. Dependence of decreasing flux with VCR can be separated in three periods. For the first, starting period, rapid decrease of filtrate flux is characteristic. Second period is defined with much smaller decrease of the flux than in the first phase. Third period has as characteristic minor decrease of flux and can be defined as steady state. Steady state emerges after τs = 80 min.
AB  - Sastojci lekovitog bilja i voća koji pokazuju potencijalnu antioksidativnu sposobnost su različite bioaktivne komponente, kao što su različiti fenoli, polifenoli, fenolne kiseline, flavonoidi, tanini i dr. Na tržištu se nalaze biljni ekstrakti od 12, 25 pa sve do 56 lekovitih i aromatičnih biljaka i voća. Dodatak medicinske gljive Ganoderma lucidum, biljnom ekstraktu od 54 lekovitih i aromatičnih biljaka, predstavlja dobro rešenje. Ekstrakcijom gljive Ganoderma (Ganoderma lucidum) zajedno sa lekovitim i aromatičnim biljem, zadržavaju se sve aktivne vrednosti biljaka, uz postizanje dodatnih lekovitih svojstava gljive. Ovakav proizvod, na bazi ekstrakata lekovitog i aromatičnog bilja, voća i gljive Ganoderma, je interesantan sa aspekta mogućih lekovitih svojstava. On može biti deklarisan ili kao gorki biljni liker u komercijalnoj prodaji ili čak kao dijetetski suplement za prodaju u apotekama. Takav proizvod u svakom slučaju predstavlja, kako zdravstveno tako i nutritivno, bolje rešenje od drugih alkoholnih pića. U radu su prikazani načini ekstrakcije i mikrofiltracije 46 lekovitih i aromatičnih biljaka, gljive Ganoderma lucidum i 8 voćnih vrsta koji čine kompoziciju Bittera 55. Utvrdjeno je da gorki biljni liker - Bitter 55 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je DPPH testom, metodom po Blois-u.1 Vrednost EC50 uzorka BITTER-a 55 koji je stajao u zatamnjenoj boci u mraku je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 redom 6,2 i 6,8 μg/ml. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju DPPH radikala (praćeno preko inhibicije apsorpcije rastvora DPPH na 517nm).
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process
T1  - Mikrofiltracija i antioksidativni kapacitet kompozicije ekstrakta lekovitih biljaka, voća i medicinske gljive Ganoderma lucidum
EP  - 62
IS  - 1
SP  - 45
VL  - 54
DO  - 10.2298/JAS0901045V
ER  - 

@article{
author = "Vukosavljević, Predrag and Novaković, Miroslav and Bukvić, Branka and Nikšić, Miomir and Stanisavljević, Ivana and Klaus, Anita",
year = "2009",
abstract = "This paper presents kinds of extraction and cross-flow filtration of composition of 46 healthful and aromatic herbs, 8 fruits and fungi Ganoderma lucidum. Those extracts are part of Bitter 55, which have significant antioxidant capacity. Antioxidative activities of plant extracts have been determined by DPPH test using method of Blois. Bitter 55 which was kept at the green bottle in the dark has EC50 = 141.07 μl/ml and it was stable during 150 days. Synthetic anti-oxidants BHT (ditertbutilhydroxytoluen, EC50 = 6.2 μgml-1), trolox (vitamin E analog soluble in water, EC50 = 6.8 μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50 %. Bitter 55 contents 35% vol of alcohol (wheat origin), 88.22 g/l total extract and slice of medicinal mushroom Ganoderma lucidum (1 % w/v) which was extracted 30 days before analyzes. The main problem in practical applications of MF is the reduction of permeate flux with time, caused by the accumulation of feed components in the pores. During microfiltration bitter herbal liquor, the function of filtrate flux is decreased with VCR. Dependence of decreasing flux with VCR can be separated in three periods. For the first, starting period, rapid decrease of filtrate flux is characteristic. Second period is defined with much smaller decrease of the flux than in the first phase. Third period has as characteristic minor decrease of flux and can be defined as steady state. Steady state emerges after τs = 80 min., Sastojci lekovitog bilja i voća koji pokazuju potencijalnu antioksidativnu sposobnost su različite bioaktivne komponente, kao što su različiti fenoli, polifenoli, fenolne kiseline, flavonoidi, tanini i dr. Na tržištu se nalaze biljni ekstrakti od 12, 25 pa sve do 56 lekovitih i aromatičnih biljaka i voća. Dodatak medicinske gljive Ganoderma lucidum, biljnom ekstraktu od 54 lekovitih i aromatičnih biljaka, predstavlja dobro rešenje. Ekstrakcijom gljive Ganoderma (Ganoderma lucidum) zajedno sa lekovitim i aromatičnim biljem, zadržavaju se sve aktivne vrednosti biljaka, uz postizanje dodatnih lekovitih svojstava gljive. Ovakav proizvod, na bazi ekstrakata lekovitog i aromatičnog bilja, voća i gljive Ganoderma, je interesantan sa aspekta mogućih lekovitih svojstava. On može biti deklarisan ili kao gorki biljni liker u komercijalnoj prodaji ili čak kao dijetetski suplement za prodaju u apotekama. Takav proizvod u svakom slučaju predstavlja, kako zdravstveno tako i nutritivno, bolje rešenje od drugih alkoholnih pića. U radu su prikazani načini ekstrakcije i mikrofiltracije 46 lekovitih i aromatičnih biljaka, gljive Ganoderma lucidum i 8 voćnih vrsta koji čine kompoziciju Bittera 55. Utvrdjeno je da gorki biljni liker - Bitter 55 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je DPPH testom, metodom po Blois-u.1 Vrednost EC50 uzorka BITTER-a 55 koji je stajao u zatamnjenoj boci u mraku je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 redom 6,2 i 6,8 μg/ml. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju DPPH radikala (praćeno preko inhibicije apsorpcije rastvora DPPH na 517nm).",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process, Mikrofiltracija i antioksidativni kapacitet kompozicije ekstrakta lekovitih biljaka, voća i medicinske gljive Ganoderma lucidum",
pages = "62-45",
number = "1",
volume = "54",
doi = "10.2298/JAS0901045V"
}

Vukosavljević, P., Novaković, M., Bukvić, B., Nikšić, M., Stanisavljević, I.,& Klaus, A.. (2009). Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 54(1), 45-62.
https://doi.org/10.2298/JAS0901045V

Vukosavljević P, Novaković M, Bukvić B, Nikšić M, Stanisavljević I, Klaus A. Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process. in Journal of Agricultural Sciences (Belgrade). 2009;54(1):45-62.
doi:10.2298/JAS0901045V .

Vukosavljević, Predrag, Novaković, Miroslav, Bukvić, Branka, Nikšić, Miomir, Stanisavljević, Ivana, Klaus, Anita, "Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process" in Journal of Agricultural Sciences (Belgrade), 54, no. 1 (2009):45-62,
https://doi.org/10.2298/JAS0901045V . .