TY  - JOUR
AU  - Kovincic, Anika
AU  - Markovic, Ksenija
AU  - Ristic, Danijela
AU  - Babic, Vojka
AU  - Petrovic, Tanja
AU  - Zivanovic, Tomislav
AU  - Kravic, Natalija
PY  - 2023
UR  - http://www.ncbi.nlm.nih.gov/pubmed/37372375
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6395
AB  - A high level of genetic purity in crop varieties must be achieved and maintained for agronomic performance, encouraging investment and innovation in plant breeding and ensuring that the improvements in productivity and quality imparted by breeders are delivered to the consumer. Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines, in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model system to examine the discriminative power of morphological, biochemical and SSR markers for seed purity assay. The highest number of off-type plants was estimated by morphological markers. According to the comparison of prolamins and albumins banding patterns of parental and derived F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer pair ability to detect non-specific bands (i.e., off-types), in both the maternal component and F1exp, which is the first report on this issue yet, strongly supports the recommendation of this SSR marker use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing.
T2  - Genes
T2  - GenesGenes (Basel)
T1  - Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation
IS  - 6
SP  - 1195
VL  - 14
DO  - 10.3390/genes14061195
ER  - 

@article{
author = "Kovincic, Anika and Markovic, Ksenija and Ristic, Danijela and Babic, Vojka and Petrovic, Tanja and Zivanovic, Tomislav and Kravic, Natalija",
year = "2023",
abstract = "A high level of genetic purity in crop varieties must be achieved and maintained for agronomic performance, encouraging investment and innovation in plant breeding and ensuring that the improvements in productivity and quality imparted by breeders are delivered to the consumer. Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines, in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model system to examine the discriminative power of morphological, biochemical and SSR markers for seed purity assay. The highest number of off-type plants was estimated by morphological markers. According to the comparison of prolamins and albumins banding patterns of parental and derived F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer pair ability to detect non-specific bands (i.e., off-types), in both the maternal component and F1exp, which is the first report on this issue yet, strongly supports the recommendation of this SSR marker use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing.",
journal = "Genes, GenesGenes (Basel)",
title = "Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation",
number = "6",
pages = "1195",
volume = "14",
doi = "10.3390/genes14061195"
}

Kovincic, A., Markovic, K., Ristic, D., Babic, V., Petrovic, T., Zivanovic, T.,& Kravic, N.. (2023). Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation. in Genes, 14(6), 1195.
https://doi.org/10.3390/genes14061195

Kovincic A, Markovic K, Ristic D, Babic V, Petrovic T, Zivanovic T, Kravic N. Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation. in Genes. 2023;14(6):1195.
doi:10.3390/genes14061195 .

Kovincic, Anika, Markovic, Ksenija, Ristic, Danijela, Babic, Vojka, Petrovic, Tanja, Zivanovic, Tomislav, Kravic, Natalija, "Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation" in Genes, 14, no. 6 (2023):1195,
https://doi.org/10.3390/genes14061195 . .