TY  - JOUR
AU  - Zhu, Gengrui
AU  - Wang, Shunli
AU  - Zhen, Shoumin
AU  - Shen, Xixi
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3839
AB  - Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
EP  - 203
IS  - 1
SP  - 185
VL  - 47
DO  - 10.2298/GENSR1501185Z
ER  - 

@article{
author = "Zhu, Gengrui and Wang, Shunli and Zhen, Shoumin and Shen, Xixi and Prodanović, Slaven and Yan, Yueming",
year = "2015",
abstract = "Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima",
pages = "203-185",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501185Z"
}

Zhu, G., Wang, S., Zhen, S., Shen, X., Prodanović, S.,& Yan, Y.. (2015). Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 185-203.
https://doi.org/10.2298/GENSR1501185Z

Zhu G, Wang S, Zhen S, Shen X, Prodanović S, Yan Y. Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika. 2015;47(1):185-203.
doi:10.2298/GENSR1501185Z .

Zhu, Gengrui, Wang, Shunli, Zhen, Shoumin, Shen, Xixi, Prodanović, Slaven, Yan, Yueming, "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima" in Genetika, 47, no. 1 (2015):185-203,
https://doi.org/10.2298/GENSR1501185Z . .

TY  - JOUR
AU  - Wang, S.L.
AU  - Chen, D.
AU  - Guo, G.F.
AU  - Zhang, T.
AU  - Jiang, S.S.
AU  - Shen, X.X.
AU  - Perović, D.
AU  - Prodanović, Slaven
AU  - Yan, Y.M.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3038
AB  - In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species
EP  - 551
IS  - 4
SP  - 542
VL  - 40
DO  - 10.1556/CRC.40.2012.0006
ER  - 

@article{
author = "Wang, S.L. and Chen, D. and Guo, G.F. and Zhang, T. and Jiang, S.S. and Shen, X.X. and Perović, D. and Prodanović, Slaven and Yan, Y.M.",
year = "2012",
abstract = "In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species",
pages = "551-542",
number = "4",
volume = "40",
doi = "10.1556/CRC.40.2012.0006"
}

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, S.,& Yan, Y.M.. (2012). Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 40(4), 542-551.
https://doi.org/10.1556/CRC.40.2012.0006

Wang S, Chen D, Guo G, Zhang T, Jiang S, Shen X, Perović D, Prodanović S, Yan Y. Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications. 2012;40(4):542-551.
doi:10.1556/CRC.40.2012.0006 .

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, Slaven, Yan, Y.M., "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species" in Cereal Research Communications, 40, no. 4 (2012):542-551,
https://doi.org/10.1556/CRC.40.2012.0006 . .