TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Dukić, Nataša
AU  - Djekić, Ivana
AU  - Krstić, Branka
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1485
AB  - Identification of Alternaria genus species is a very complicated process which demands broadly designed investigations and studying of great number of properties which together can be considered as satisfying taxonomic criteria. The main objective of these investigations was examining the possibilities of applying the antigenic characteristics of Alternaria spp. phytopathogenic fungi as a taxonomic criterion, as well as introducing the serological methods for their identification. Conducting the examination of Alternaria spp., pathogenic for Apiaceae plants in Serbia, several isolates were obtained and identified as Alternaria radicina, A. petroselini, A. dauci and A. alternata, based on the conventional mycological methods and host range, as well as on molecular detection and partial characterization. The investigation included 12 isolates from plant leaves, seeds and soil which were pathogenic mainly to carrot and parsley and were identified as A. radicina, A. petroselini, A. dauci and A. alternate. Investigated isolates were compared with each other, as well as with standard isolates for the mentioned species (a total of 5 isolates, originating from USA and EU). During the investigation of serological characteristics of Alternaria spp. firstly a polyclonal antiserum was prepared against one isolate from Serbia identified as A. dauci. This antiserum was specific to Alternaria genus while there was no reaction with antigens from other phytopathogenic fungi genera (Fusarium, Rhizoctonia and Agaricus). Antiserum titer, determined by slide agglutination test, was 1/32. Antigenic characteristics of Alternaria genus fungi were examined by Electro-Blot-Immunoassay serological method (EBIA, Western blot), i.e. their protein profiles were compared. Investigated Alternaria spp. isolates showed different protein band profiles in gel and on nitrocellulose paper, and the observed differences were in complete correlation with the results of the previous identification. All investigated isolates, both domestic and the standards, were similar to each other, and they could be correctly identified to the species level using EBIA. Besides grouping to the species level, antigenic characteristics indicated similarities and differences among the isolates within the same and different species, showing their complex relationships which properly reflect their diversity in nature. In all the previous investigations of Alternaria genus fungi up to now, there have been no data about their serological characteristics as possible taxonomic criteria. Introduction of this group of characteristics represents an important contribution both to the taxonomy and implementation of fast and accurate methods of phytopathogenic fungi identification.
AB  - Identifikacija vrsta u okviru roda Alternaria je veoma složen proces koji zahteva široko postavljena ispitivanja i proučavanje većeg broja osobina koje zajedno predstavljaju zadovoljavajuće taksonomske kriterijume. Osnovni cilj ovog rada je bio da se ispita mogućnost primene antigenih osobina fitopatogenih gljiva iz roda Alternaria kao taksonomskog kriterijuma, kao i uvođenje seroloških metoda za njihovu identifikaciju. Proučavajući gljive iz roda Alternaria koje su patogene za gajene biljke iz fam. Apiaceae u našoj zemlji, dobijeno je više izolata koji su, na osnovu proučavanja konvencionalnim metodama i kruga domaćina, kao i molekularne detekcije i delimične karakterizacije, razvrstani u četiri vrste: Alternaria radicina A. petroselini, A. dauci i A. alternata. U ispitivanja je bilo uključeno 12 izolata poreklom sa lista, semena ili iz zemlje, koji su ispoljavali patogenost prvenstveno prema mrkvi i peršunu i identifikovani da pripadaju vrstama A. radicina, A. petroselini, A. dauci i A. alternata. Ispitivani izolati su međusobno upoređivani kao i sa standardima za navedene vrste (ukupno 5 izolata, poreklom iz SAD i EU). Prilikom ispitivanja seroloških osobina Alternaria spp., prvo je pripremljen poliklonalni antiserum na izolat iz Srbije, koji je identifikovan kao A. dauci. Antiserum je ispoljio specifičnost za rod Alternaria, dok nije reagovao sa antigenima iz drugih rodova fitopatogenih gljiva (Fusarium, Rhizoctonia, Agaricus). Titar antiseruma, ispitivan metodom aglutinacije na pločici, bio je 1/32. Antigene osobine gljiva iz roda Alternaria proučavane su primenom elektroforetsko-adsorpciono-imunoenzimske serološke metode (EBIA, Western blot), odnosno upoređivani su njihovi proteinski profili. Ispitivani izolati Alternaria spp. ispoljili su različite profile proteinskih traka u gelu i na nitroceluloznom papiru, a uočene razlike su, u potpunosti, odgovarale rezultatima prethodne identifikacije. Navedeni izolati, domaći kao i standardi, ispoljili su međusobne sličnosti i mogli su biti pravilno identifikovane do nivoa vrste, primenom EBIA. Pored grupisanja do nivoa vrsta, antigene osobine su ukazale na postojanje sličnosti i razlika između izolata unutar istih, odnosno različitih vrsta, ukazujući na njihove veoma složene međuodnose koji verno oslikavaju diverzitet postojanja ovih gljiva u prirodi. U dosadašnjim proučavanjima gljiva iz roda Alternaria, ne postoje podaci o njihovim serološkim osobinama kao mogućim taksonomskim kriterijumima. Uvođenje ove grupe osobina, predstavlja značajan doprinos kako u taksonomiji, tako i u primeni brzih i tačnih metoda identifikacije fitopatogenih gljiva.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley
T1  - Antigene osobine kao taksonomski kriterijum za razlikovanje Alternaria spp. patogenih za mrkvu i peršun
EP  - 154
IS  - 113
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1485
ER  - 

@article{
author = "Bulajić, Aleksandra and Dukić, Nataša and Djekić, Ivana and Krstić, Branka",
year = "2007",
abstract = "Identification of Alternaria genus species is a very complicated process which demands broadly designed investigations and studying of great number of properties which together can be considered as satisfying taxonomic criteria. The main objective of these investigations was examining the possibilities of applying the antigenic characteristics of Alternaria spp. phytopathogenic fungi as a taxonomic criterion, as well as introducing the serological methods for their identification. Conducting the examination of Alternaria spp., pathogenic for Apiaceae plants in Serbia, several isolates were obtained and identified as Alternaria radicina, A. petroselini, A. dauci and A. alternata, based on the conventional mycological methods and host range, as well as on molecular detection and partial characterization. The investigation included 12 isolates from plant leaves, seeds and soil which were pathogenic mainly to carrot and parsley and were identified as A. radicina, A. petroselini, A. dauci and A. alternate. Investigated isolates were compared with each other, as well as with standard isolates for the mentioned species (a total of 5 isolates, originating from USA and EU). During the investigation of serological characteristics of Alternaria spp. firstly a polyclonal antiserum was prepared against one isolate from Serbia identified as A. dauci. This antiserum was specific to Alternaria genus while there was no reaction with antigens from other phytopathogenic fungi genera (Fusarium, Rhizoctonia and Agaricus). Antiserum titer, determined by slide agglutination test, was 1/32. Antigenic characteristics of Alternaria genus fungi were examined by Electro-Blot-Immunoassay serological method (EBIA, Western blot), i.e. their protein profiles were compared. Investigated Alternaria spp. isolates showed different protein band profiles in gel and on nitrocellulose paper, and the observed differences were in complete correlation with the results of the previous identification. All investigated isolates, both domestic and the standards, were similar to each other, and they could be correctly identified to the species level using EBIA. Besides grouping to the species level, antigenic characteristics indicated similarities and differences among the isolates within the same and different species, showing their complex relationships which properly reflect their diversity in nature. In all the previous investigations of Alternaria genus fungi up to now, there have been no data about their serological characteristics as possible taxonomic criteria. Introduction of this group of characteristics represents an important contribution both to the taxonomy and implementation of fast and accurate methods of phytopathogenic fungi identification., Identifikacija vrsta u okviru roda Alternaria je veoma složen proces koji zahteva široko postavljena ispitivanja i proučavanje većeg broja osobina koje zajedno predstavljaju zadovoljavajuće taksonomske kriterijume. Osnovni cilj ovog rada je bio da se ispita mogućnost primene antigenih osobina fitopatogenih gljiva iz roda Alternaria kao taksonomskog kriterijuma, kao i uvođenje seroloških metoda za njihovu identifikaciju. Proučavajući gljive iz roda Alternaria koje su patogene za gajene biljke iz fam. Apiaceae u našoj zemlji, dobijeno je više izolata koji su, na osnovu proučavanja konvencionalnim metodama i kruga domaćina, kao i molekularne detekcije i delimične karakterizacije, razvrstani u četiri vrste: Alternaria radicina A. petroselini, A. dauci i A. alternata. U ispitivanja je bilo uključeno 12 izolata poreklom sa lista, semena ili iz zemlje, koji su ispoljavali patogenost prvenstveno prema mrkvi i peršunu i identifikovani da pripadaju vrstama A. radicina, A. petroselini, A. dauci i A. alternata. Ispitivani izolati su međusobno upoređivani kao i sa standardima za navedene vrste (ukupno 5 izolata, poreklom iz SAD i EU). Prilikom ispitivanja seroloških osobina Alternaria spp., prvo je pripremljen poliklonalni antiserum na izolat iz Srbije, koji je identifikovan kao A. dauci. Antiserum je ispoljio specifičnost za rod Alternaria, dok nije reagovao sa antigenima iz drugih rodova fitopatogenih gljiva (Fusarium, Rhizoctonia, Agaricus). Titar antiseruma, ispitivan metodom aglutinacije na pločici, bio je 1/32. Antigene osobine gljiva iz roda Alternaria proučavane su primenom elektroforetsko-adsorpciono-imunoenzimske serološke metode (EBIA, Western blot), odnosno upoređivani su njihovi proteinski profili. Ispitivani izolati Alternaria spp. ispoljili su različite profile proteinskih traka u gelu i na nitroceluloznom papiru, a uočene razlike su, u potpunosti, odgovarale rezultatima prethodne identifikacije. Navedeni izolati, domaći kao i standardi, ispoljili su međusobne sličnosti i mogli su biti pravilno identifikovane do nivoa vrste, primenom EBIA. Pored grupisanja do nivoa vrsta, antigene osobine su ukazale na postojanje sličnosti i razlika između izolata unutar istih, odnosno različitih vrsta, ukazujući na njihove veoma složene međuodnose koji verno oslikavaju diverzitet postojanja ovih gljiva u prirodi. U dosadašnjim proučavanjima gljiva iz roda Alternaria, ne postoje podaci o njihovim serološkim osobinama kao mogućim taksonomskim kriterijumima. Uvođenje ove grupe osobina, predstavlja značajan doprinos kako u taksonomiji, tako i u primeni brzih i tačnih metoda identifikacije fitopatogenih gljiva.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley, Antigene osobine kao taksonomski kriterijum za razlikovanje Alternaria spp. patogenih za mrkvu i peršun",
pages = "154-143",
number = "113",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1485"
}

Bulajić, A., Dukić, N., Djekić, I.,& Krstić, B.. (2007). Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(113), 143-154.
https://hdl.handle.net/21.15107/rcub_agrospace_1485

Bulajić A, Dukić N, Djekić I, Krstić B. Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley. in Zbornik Matice srpske za prirodne nauke. 2007;(113):143-154.
https://hdl.handle.net/21.15107/rcub_agrospace_1485 .

Bulajić, Aleksandra, Dukić, Nataša, Djekić, Ivana, Krstić, Branka, "Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley" in Zbornik Matice srpske za prirodne nauke, no. 113 (2007):143-154,
https://hdl.handle.net/21.15107/rcub_agrospace_1485 .

TY  - JOUR
AU  - Zindović, Jelena
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Latinović, Jelena
AU  - Djekić, Ivana
AU  - Duduk, Bojan
AU  - Krstić, Branka
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1467
AB  - Seven important tobacco viruses were investigated in Montenegro in 2005: Tobacco Mosaic Virus (TMV), Tomato Spotted Wilt Virus (TSWV), Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY), Alfalfa Mosaic Virus (AMV), Tobacco Ring Spot Virus (TRSV) and Potato Virus X (PVX). This investigation included sample collection from four tobacco growing regions in Montenegro and their serological testing by DAS-ELISA test. Presence of different strains of PVY was investigated as well using DAS ELISA test with specific monoclonal antibodies. Serological results proved the presence of four tobacco viruses (TMV, CMV, PVY and AMV), while TSWV, TRSV and PVX were not found in the tested samples of tobacco crops in Montenegro. The results also showed that TMV and CMV were the most frequent (44.6% and 41.5% of tested samples, respectively) followed by PVY (15.4%) and the least frequent AMV (3.1%). Most samples were infected with one of the examined viruses. In the PVY population found in Montenegro, its necrotic strain (PVYN) was absolutely predominant. The results indicated the significance of TMV and CMV concerning tobacco viral infections in Montenegro, as well as a necessity of their detailed characterization at biological and molecular level.
AB  - Istraživanja prisustva i rasprostranjenosti sedam ekonomski značajnijih virusa duvana: virusa mozaika duvana (Tobacco Mosaic Virus, TMV), virusa bronzavosti paradajza (Tomato Spotted Wilt Virus, TSWV), virusa mozaika krastavca (Cucumber Mosaic Virus, CMV), virusa crtičastog mozaika krompira (Potato Virus Y, PVY), virusa mozaika lucerke (Alfalfa Mosaic Virus, AMV), virusa prstenaste pegavosti duvana (Tobacco Ringspot Virus, TRSV) i virusa mozaika krompira (Potato Virus X, PVX) su obavljena na teritoriji Crne Gore tokom 2005. godine. Uzorci su sakupljeni u četiri različita lokaliteta gajenja duvana i testirani na prisustvo virusa primjenom DAS-ELISA testa. Serološkim analizama utvrđeno je prisustvo četiri ispitivana virusa: TMV, CMV, PVY i AMV, dok prisustvo TSWV, TRSV i PVX nije konstatovano u usjevima duvana u Crnoj Gori. Najveću zastupljenost imali su TMV (44.6%) i CMV (41.5%). Treći po raširenosti bio je PVY (15.4%), a četvrti AMV (3.1%). Takođe, primjenom DAS-ELISA testa i monoklonalnih antiseruma specifičnih za različite sojeve PVY, utvrđena je apsolutna dominantnost nekrotičnog soja (PVYN) u populaciji PVY. Rezultati ispitivanja su ukazali na značaj TMV i CMV u proizvodnji duvana u Crnoj Gori, ali i na neophodnost njihove detaljne karakterizacije na biološkom i molekularnom nivou i sprovođenje odgovarajućih mjera kontrole.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and distribution of tobacco viruses in Montenegro
T1  - Prisustvo i rasprostranjenost virusa duvana u Crnoj Gori
EP  - 44
IS  - 1
SP  - 39
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1467
ER  - 

@article{
author = "Zindović, Jelena and Dukić, Nataša and Bulajić, Aleksandra and Latinović, Jelena and Djekić, Ivana and Duduk, Bojan and Krstić, Branka",
year = "2007",
abstract = "Seven important tobacco viruses were investigated in Montenegro in 2005: Tobacco Mosaic Virus (TMV), Tomato Spotted Wilt Virus (TSWV), Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY), Alfalfa Mosaic Virus (AMV), Tobacco Ring Spot Virus (TRSV) and Potato Virus X (PVX). This investigation included sample collection from four tobacco growing regions in Montenegro and their serological testing by DAS-ELISA test. Presence of different strains of PVY was investigated as well using DAS ELISA test with specific monoclonal antibodies. Serological results proved the presence of four tobacco viruses (TMV, CMV, PVY and AMV), while TSWV, TRSV and PVX were not found in the tested samples of tobacco crops in Montenegro. The results also showed that TMV and CMV were the most frequent (44.6% and 41.5% of tested samples, respectively) followed by PVY (15.4%) and the least frequent AMV (3.1%). Most samples were infected with one of the examined viruses. In the PVY population found in Montenegro, its necrotic strain (PVYN) was absolutely predominant. The results indicated the significance of TMV and CMV concerning tobacco viral infections in Montenegro, as well as a necessity of their detailed characterization at biological and molecular level., Istraživanja prisustva i rasprostranjenosti sedam ekonomski značajnijih virusa duvana: virusa mozaika duvana (Tobacco Mosaic Virus, TMV), virusa bronzavosti paradajza (Tomato Spotted Wilt Virus, TSWV), virusa mozaika krastavca (Cucumber Mosaic Virus, CMV), virusa crtičastog mozaika krompira (Potato Virus Y, PVY), virusa mozaika lucerke (Alfalfa Mosaic Virus, AMV), virusa prstenaste pegavosti duvana (Tobacco Ringspot Virus, TRSV) i virusa mozaika krompira (Potato Virus X, PVX) su obavljena na teritoriji Crne Gore tokom 2005. godine. Uzorci su sakupljeni u četiri različita lokaliteta gajenja duvana i testirani na prisustvo virusa primjenom DAS-ELISA testa. Serološkim analizama utvrđeno je prisustvo četiri ispitivana virusa: TMV, CMV, PVY i AMV, dok prisustvo TSWV, TRSV i PVX nije konstatovano u usjevima duvana u Crnoj Gori. Najveću zastupljenost imali su TMV (44.6%) i CMV (41.5%). Treći po raširenosti bio je PVY (15.4%), a četvrti AMV (3.1%). Takođe, primjenom DAS-ELISA testa i monoklonalnih antiseruma specifičnih za različite sojeve PVY, utvrđena je apsolutna dominantnost nekrotičnog soja (PVYN) u populaciji PVY. Rezultati ispitivanja su ukazali na značaj TMV i CMV u proizvodnji duvana u Crnoj Gori, ali i na neophodnost njihove detaljne karakterizacije na biološkom i molekularnom nivou i sprovođenje odgovarajućih mjera kontrole.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and distribution of tobacco viruses in Montenegro, Prisustvo i rasprostranjenost virusa duvana u Crnoj Gori",
pages = "44-39",
number = "1",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1467"
}

Zindović, J., Dukić, N., Bulajić, A., Latinović, J., Djekić, I., Duduk, B.,& Krstić, B.. (2007). Presence and distribution of tobacco viruses in Montenegro. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 22(1), 39-44.
https://hdl.handle.net/21.15107/rcub_agrospace_1467

Zindović J, Dukić N, Bulajić A, Latinović J, Djekić I, Duduk B, Krstić B. Presence and distribution of tobacco viruses in Montenegro. in Pesticidi i fitomedicina. 2007;22(1):39-44.
https://hdl.handle.net/21.15107/rcub_agrospace_1467 .

Zindović, Jelena, Dukić, Nataša, Bulajić, Aleksandra, Latinović, Jelena, Djekić, Ivana, Duduk, Bojan, Krstić, Branka, "Presence and distribution of tobacco viruses in Montenegro" in Pesticidi i fitomedicina, 22, no. 1 (2007):39-44,
https://hdl.handle.net/21.15107/rcub_agrospace_1467 .

TY  - JOUR
AU  - Vico, Ivana
AU  - Janković, Dragica B.
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Dukić, Nataša
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1315
AB  - Sugar beet root rot has severely occurred in our country recently, especially in localities of Pazova, Pećinci, Ruma, Sremska Mitrovica and Šid. From diseased roots as well as from soil collected from the localities where decay occurred, fungal isolates were obtained by bait plant method. Based on their characteristics, they were identified as multinucleate Rhizoctonia sp. During the year of 2004 in Mitrosrem trial field T-11, where the presence of multinucleate Rhizoctonia sp. was confirmed, an experiment under the coordination of Committee for Acknowledgement and Registration of New Cultivars in our country was conducted in order to determine cultivars' tolerance, i.e. their susceptibility and possibility for growing on infested fields. Six cultivars of sugar beet, Laetitia (as standard) and five new ones were included in the investigation. The trial was conducted in accordance with the established and accepted method (Ministry of Agriculture, Forestry and Water Resources, Republic of Serbia). Susceptibility of investigated cultivars was evaluated according to significant production characteristics root yield, sugar content, corrected sugar content, thick juice Q, molasses sugar, content of K, Na and amino-N, polarized sugar yield and white sugar yield, as it was recommended by the method. Conducted investigations have revealed that tested sugar beet cultivars showed different reactions to natural infection with multinucleate Rhizoctonia sp. Concerning root yield as the most important agricultural characteristic, statistically significantly higher yield was obtained with the cultivar under code mark 5 (61.120 kg/ha) whereas the cultivar marked under code 6 had significantly lower yield comparing to the standard (38.100 kg/ha).
AB  - Poslednjih godina uočeno je masovno propadanje šećerne repe u našoj zemlji i to u lokalitetima Pazove, Pećinaca, Rume, Sremske Mitrovice i Šida. Iz obolelih korenova i iz zemljišta prikupljenog sa terena gde je propadanje uočeno, metodom mamaka izolovana je gljiva koja je po svojim osobinama identifikovana kao višejedarna Rhizoctonia sp. U toku 2004. godine na parceli Mitrosrema T-11 gde je izolacijom dokazano prisustvo višejedarne Rhizoctonia sp., postavljen je ogled u okviru sortne komisije za priznavanje i registraciju novih sorti u našoj zemlji sa ciljem utvrđivanja tolerantnosti, odnosno osetljivosti pojedinih sorti i time njihove pogodnosti za gajenje na infestiranom zemljištu. U ispitivanja je uključeno 6 sorti šećerne repe: Laetitia (kao standard) i još pet novih sorti. Ogled je posejan po utvrđenoj i prihvaćenoj metodi sortne komisije (Ministarstvo poljoprivrede, šumarstva i vodoprivrede Republike Srbije). Osetljivost ispitivanih sorti ocenjivana je na osnovu značajnih proizvodnih osobina: prinos korena, polarizacija, korigovani sadržaj šećera Q gustog soka, sadržaja sećera u melasi, sadržaja K, Na i amino N, prinos polarizacionog šećera i prinos kristalnog šećera, kako to metoda i zahteva. Na osnovu obavljenih istraživanja ustanovljeno je da se ispitivane sorte šećerne repe različito ponašaju u uslovima prirodne zaraze višejedarnom Rhizoctonia sp. U pogledu prinosa, kao najvažnije proizvodne karakteristike, statistički značajno viši prinos od standarda ispoljila je sorta koja se vodi pod šifrom 5 (61.120 kg/ha), dok je sorta koja se vodi pod šifrom 6 imala statistički značajno niži prinos u poređenju sa standardom (38.100 kg/ha).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions
T1  - Višejedarna Rhizoctonia sp. - patogen šećerne repe i osetljivost sorti u polju
EP  - 121
IS  - 110
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1315
ER  - 

@article{
author = "Vico, Ivana and Janković, Dragica B. and Krstić, Branka and Bulajić, Aleksandra and Dukić, Nataša",
year = "2006",
abstract = "Sugar beet root rot has severely occurred in our country recently, especially in localities of Pazova, Pećinci, Ruma, Sremska Mitrovica and Šid. From diseased roots as well as from soil collected from the localities where decay occurred, fungal isolates were obtained by bait plant method. Based on their characteristics, they were identified as multinucleate Rhizoctonia sp. During the year of 2004 in Mitrosrem trial field T-11, where the presence of multinucleate Rhizoctonia sp. was confirmed, an experiment under the coordination of Committee for Acknowledgement and Registration of New Cultivars in our country was conducted in order to determine cultivars' tolerance, i.e. their susceptibility and possibility for growing on infested fields. Six cultivars of sugar beet, Laetitia (as standard) and five new ones were included in the investigation. The trial was conducted in accordance with the established and accepted method (Ministry of Agriculture, Forestry and Water Resources, Republic of Serbia). Susceptibility of investigated cultivars was evaluated according to significant production characteristics root yield, sugar content, corrected sugar content, thick juice Q, molasses sugar, content of K, Na and amino-N, polarized sugar yield and white sugar yield, as it was recommended by the method. Conducted investigations have revealed that tested sugar beet cultivars showed different reactions to natural infection with multinucleate Rhizoctonia sp. Concerning root yield as the most important agricultural characteristic, statistically significantly higher yield was obtained with the cultivar under code mark 5 (61.120 kg/ha) whereas the cultivar marked under code 6 had significantly lower yield comparing to the standard (38.100 kg/ha)., Poslednjih godina uočeno je masovno propadanje šećerne repe u našoj zemlji i to u lokalitetima Pazove, Pećinaca, Rume, Sremske Mitrovice i Šida. Iz obolelih korenova i iz zemljišta prikupljenog sa terena gde je propadanje uočeno, metodom mamaka izolovana je gljiva koja je po svojim osobinama identifikovana kao višejedarna Rhizoctonia sp. U toku 2004. godine na parceli Mitrosrema T-11 gde je izolacijom dokazano prisustvo višejedarne Rhizoctonia sp., postavljen je ogled u okviru sortne komisije za priznavanje i registraciju novih sorti u našoj zemlji sa ciljem utvrđivanja tolerantnosti, odnosno osetljivosti pojedinih sorti i time njihove pogodnosti za gajenje na infestiranom zemljištu. U ispitivanja je uključeno 6 sorti šećerne repe: Laetitia (kao standard) i još pet novih sorti. Ogled je posejan po utvrđenoj i prihvaćenoj metodi sortne komisije (Ministarstvo poljoprivrede, šumarstva i vodoprivrede Republike Srbije). Osetljivost ispitivanih sorti ocenjivana je na osnovu značajnih proizvodnih osobina: prinos korena, polarizacija, korigovani sadržaj šećera Q gustog soka, sadržaja sećera u melasi, sadržaja K, Na i amino N, prinos polarizacionog šećera i prinos kristalnog šećera, kako to metoda i zahteva. Na osnovu obavljenih istraživanja ustanovljeno je da se ispitivane sorte šećerne repe različito ponašaju u uslovima prirodne zaraze višejedarnom Rhizoctonia sp. U pogledu prinosa, kao najvažnije proizvodne karakteristike, statistički značajno viši prinos od standarda ispoljila je sorta koja se vodi pod šifrom 5 (61.120 kg/ha), dok je sorta koja se vodi pod šifrom 6 imala statistički značajno niži prinos u poređenju sa standardom (38.100 kg/ha).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions, Višejedarna Rhizoctonia sp. - patogen šećerne repe i osetljivost sorti u polju",
pages = "121-109",
number = "110",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1315"
}

Vico, I., Janković, D. B., Krstić, B., Bulajić, A.,& Dukić, N.. (2006). Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(110), 109-121.
https://hdl.handle.net/21.15107/rcub_agrospace_1315

Vico I, Janković DB, Krstić B, Bulajić A, Dukić N. Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions. in Zbornik Matice srpske za prirodne nauke. 2006;(110):109-121.
https://hdl.handle.net/21.15107/rcub_agrospace_1315 .

Vico, Ivana, Janković, Dragica B., Krstić, Branka, Bulajić, Aleksandra, Dukić, Nataša, "Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions" in Zbornik Matice srpske za prirodne nauke, no. 110 (2006):109-121,
https://hdl.handle.net/21.15107/rcub_agrospace_1315 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Dukić, Nataša
AU  - Duduk, Bojan
AU  - Berenji, Janoš
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1307
AB  - Many features of Tomato spotted wilt virus make this virus unique among plant infecting viruses. This virus is considered to be the most widespread virus in the world and that fact is attributed to the rapid expansion of its the most efficient vector Frankliniella occidentalis (Western flower thrips). Tomato spotted wilt virus is extremely polyphagous with, probably, the widest host range of all plant viruses. It causes very destructive diseases of many economically important plants, especially in tobacco, tomato, pepper and ornamentals. It is also responsible for numerous epidemics in tobacco crops in different regions of the world. Since the control of the tomato spotted wilt virus is very complicated, it makes its significance even greater. Considerable efforts have been made in developing resistance or tolerance to Tomato spotted wilt virus infections in certain tobacco cultivars. The results obtained so far in breeding for virus resistance have been disappointed because of the lack of suitable natural forms of resistance, which if there is, is in most cases polygenic and based on the complex interaction among the virus, vector and plant. That is why there is a need for new genetically engineered forms of host plant resistance. However, the extreme variability of Tomato spotted wilt virus is the main cause of breaking the natural or engineered resistance of plants. Also, several biological characteristics of the thrips and rapid development of its resistance to insecticides make its control extremely difficult. It has been discovered recently that an early treatment of tobacco plants with the combination of two chemicals (an insecticide - imidacloprid and a plant defense activator - acibenzolar-S-methyl) could reduce or eliminate the symptoms of Tomato spotted wilt virus on tobacco. This, for the first time, gives the possibility of true control. Good knowledge of the biology and epidemiology of both the virus and its vector is essential for its successful control. An adequate control of Tomato spotted wilt virus can be achieved by integrated pest management including a combination of certain physical, cultural and chemical measures.
AB  - Virus bronzavosti paradajza (Tomato spotted wilt virus) je, po mnogim svojim osobinama, jedinstven u odnosu na druge biljne viruse. Smatra se da je najrasprostranjeniji virus u svetu, zahvaljujući brzoj ekspanziji svog najefikasnijeg vektora - kalifornijskog tripsa, Frankliniella occidentalis. Virus bronzavosti paradajza je izrazito polifagan sa, verovatno, najširim krugom domaćina među svim biljnim virusima. Prouzrokuje veoma destruktivna oboljenja mnogih ekonomski važnih gajenih biljaka, a pre svega duvana, paradajza, paprike i ukrasnih biljaka. Na duvanu je izazvao brojne epidemije u različitim delovima sveta. Kontrola oboljenja koje ovaj virus izaziva na duvanu veoma je složena, što čini značaj ovog virusa još većim. Učinjeni su značajni napori da se dobiju rezistentni ili tolerantni kultivari duvana zaraze ovim virusom, ali su rezultati selekcije na otpornost razočaravajući. Razlog leži u nedostatku odgovarajuće forme prirodne otpornosti koja je, ako je uopšte ima, poligenetske prirode i zasniva se na kompleksnoj interakciji virusa, vektora i biljke. Zbog toga se ukazala potreba za iznalaženjem novih vidova otpornosti putem genetičkog inženjerstva. Međutim, teškoće u kontroli pričinjava i velika varijabilnost virusa, što uslovljava brzo slamanje otpornosti biljaka, bilo da je dobijena prirodnim putem ili genetičkim inženjerstvom. Takođe, neke biološke karakteristike tripsa i brzo razvijanje rezistentnosti prema insekticidima, čine kontrolu virusa izrazito teškom. Nedavno je otkriveno da dva jedinjenja, insekticid-imidacloprid i aktivator otpornosti biljaka - acibenzolar-S-methyl, ako se primene zajedno u ranim fazama razvoja duvana, mogu da smanje ili potpuno eliminišu simptome koje ovaj virus izaziva na duvanu. Ovo otkriće pruža mogućnost prave kontrole po prvi put. Uspešna kontrola oboljenja koje ovaj virus izaziva na duvanu podrazumeva dobro razumevanje biologije i epidemiologije i virusa i tripsa kao vektora. Sprovođenjem integralnog programa zaštite kombinacijom određenih fizičkih, agrotehničkih i hemijskih mera može se postići odgovarajuća kontrola virusa bronzavosti paradajza na duvanu.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Integrated pest management of tomato spotted wilt virus in tobacco
T1  - Integralna zaštita duvana od virusa bronzavosti paradajza
EP  - 60
IS  - 79
SP  - 49
VL  - 38
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1307
ER  - 

@article{
author = "Krstić, Branka and Bulajić, Aleksandra and Dukić, Nataša and Duduk, Bojan and Berenji, Janoš",
year = "2006",
abstract = "Many features of Tomato spotted wilt virus make this virus unique among plant infecting viruses. This virus is considered to be the most widespread virus in the world and that fact is attributed to the rapid expansion of its the most efficient vector Frankliniella occidentalis (Western flower thrips). Tomato spotted wilt virus is extremely polyphagous with, probably, the widest host range of all plant viruses. It causes very destructive diseases of many economically important plants, especially in tobacco, tomato, pepper and ornamentals. It is also responsible for numerous epidemics in tobacco crops in different regions of the world. Since the control of the tomato spotted wilt virus is very complicated, it makes its significance even greater. Considerable efforts have been made in developing resistance or tolerance to Tomato spotted wilt virus infections in certain tobacco cultivars. The results obtained so far in breeding for virus resistance have been disappointed because of the lack of suitable natural forms of resistance, which if there is, is in most cases polygenic and based on the complex interaction among the virus, vector and plant. That is why there is a need for new genetically engineered forms of host plant resistance. However, the extreme variability of Tomato spotted wilt virus is the main cause of breaking the natural or engineered resistance of plants. Also, several biological characteristics of the thrips and rapid development of its resistance to insecticides make its control extremely difficult. It has been discovered recently that an early treatment of tobacco plants with the combination of two chemicals (an insecticide - imidacloprid and a plant defense activator - acibenzolar-S-methyl) could reduce or eliminate the symptoms of Tomato spotted wilt virus on tobacco. This, for the first time, gives the possibility of true control. Good knowledge of the biology and epidemiology of both the virus and its vector is essential for its successful control. An adequate control of Tomato spotted wilt virus can be achieved by integrated pest management including a combination of certain physical, cultural and chemical measures., Virus bronzavosti paradajza (Tomato spotted wilt virus) je, po mnogim svojim osobinama, jedinstven u odnosu na druge biljne viruse. Smatra se da je najrasprostranjeniji virus u svetu, zahvaljujući brzoj ekspanziji svog najefikasnijeg vektora - kalifornijskog tripsa, Frankliniella occidentalis. Virus bronzavosti paradajza je izrazito polifagan sa, verovatno, najširim krugom domaćina među svim biljnim virusima. Prouzrokuje veoma destruktivna oboljenja mnogih ekonomski važnih gajenih biljaka, a pre svega duvana, paradajza, paprike i ukrasnih biljaka. Na duvanu je izazvao brojne epidemije u različitim delovima sveta. Kontrola oboljenja koje ovaj virus izaziva na duvanu veoma je složena, što čini značaj ovog virusa još većim. Učinjeni su značajni napori da se dobiju rezistentni ili tolerantni kultivari duvana zaraze ovim virusom, ali su rezultati selekcije na otpornost razočaravajući. Razlog leži u nedostatku odgovarajuće forme prirodne otpornosti koja je, ako je uopšte ima, poligenetske prirode i zasniva se na kompleksnoj interakciji virusa, vektora i biljke. Zbog toga se ukazala potreba za iznalaženjem novih vidova otpornosti putem genetičkog inženjerstva. Međutim, teškoće u kontroli pričinjava i velika varijabilnost virusa, što uslovljava brzo slamanje otpornosti biljaka, bilo da je dobijena prirodnim putem ili genetičkim inženjerstvom. Takođe, neke biološke karakteristike tripsa i brzo razvijanje rezistentnosti prema insekticidima, čine kontrolu virusa izrazito teškom. Nedavno je otkriveno da dva jedinjenja, insekticid-imidacloprid i aktivator otpornosti biljaka - acibenzolar-S-methyl, ako se primene zajedno u ranim fazama razvoja duvana, mogu da smanje ili potpuno eliminišu simptome koje ovaj virus izaziva na duvanu. Ovo otkriće pruža mogućnost prave kontrole po prvi put. Uspešna kontrola oboljenja koje ovaj virus izaziva na duvanu podrazumeva dobro razumevanje biologije i epidemiologije i virusa i tripsa kao vektora. Sprovođenjem integralnog programa zaštite kombinacijom određenih fizičkih, agrotehničkih i hemijskih mera može se postići odgovarajuća kontrola virusa bronzavosti paradajza na duvanu.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Integrated pest management of tomato spotted wilt virus in tobacco, Integralna zaštita duvana od virusa bronzavosti paradajza",
pages = "60-49",
number = "79",
volume = "38",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1307"
}

Krstić, B., Bulajić, A., Dukić, N., Duduk, B.,& Berenji, J.. (2006). Integrated pest management of tomato spotted wilt virus in tobacco. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 38(79), 49-60.
https://hdl.handle.net/21.15107/rcub_agrospace_1307

Krstić B, Bulajić A, Dukić N, Duduk B, Berenji J. Integrated pest management of tomato spotted wilt virus in tobacco. in Bilten za hmelj, sirak i lekovito bilje. 2006;38(79):49-60.
https://hdl.handle.net/21.15107/rcub_agrospace_1307 .

Krstić, Branka, Bulajić, Aleksandra, Dukić, Nataša, Duduk, Bojan, Berenji, Janoš, "Integrated pest management of tomato spotted wilt virus in tobacco" in Bilten za hmelj, sirak i lekovito bilje, 38, no. 79 (2006):49-60,
https://hdl.handle.net/21.15107/rcub_agrospace_1307 .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Dukić, Nataša
AU  - Duduk, Bojan
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1221
AB  - Puccinia horiana, the cause of chrysanthemum white rust, is economically a very important fungus, which is widely distributed in almost all chrysanthemum growing regions. The presence of P. horiana in our country was last reported nearly 20 years ago. This pathogen is a quarantine pest, included on the SCG A2 list as well as the EPPO A2 list. In 2005 an intensive appearance of chrysanthemum white rust was recorded in the locality of Vinca. Identification of this obligate pathogen was performed by studying disease symptoms and the pathogen's morphological features. On the upper leaf surface numerous pale green to yellow sunken spots, 1-2 mm in diameter on the overage were noticeable. On the corresponding lower leaf surface, a buff of whitish pustules, telia, were found. Severely attacked leaves become yellowish, wilt and gradually dry up completely. In the whitish telia, formed on the lower leaf surface, the characteristic smooth, hyaline 2-celled teliospores with average dimensions of 41.25 x 15μm were established. Teiispores are on pedicel and slightly constricted along septae. The upper cell is thicker at the apex. Teliospores easily germinate in situ under moist conditions. About 15 days after artificial inoculation, chrysanthemum cut­tings reacted with characteristic symptoms. In order to prevent appearance or further spreading of P. horiana in certain regions, quarantine and other phytosanitary measures are recommended. These measures include: routine nursery inspections, in case of a disease outbreak, crop within the facility must be destroyed completely or partially, while surrounding susceptible plants are to be protected according to "three treatment protocol". Following strict appropriate measures, complete eradication of P. horiana from a certain region can be accomplished. Chemical treatment schedule should include fungicides with different modes of action and their rotation, in order to avoid development of pathogen resistance. In this investigation, reappearance of P. horiana, the causal agent of chrysanthemum white rust, was confirmed in the locality of Vinca, in open field chrysanthemum production. Control measure recommendations were summarized as well, in order to protect chrysanthemum production in our country. .
AB  - Puccinia horiana, prouzrokovač bele rđe hrizanteme, ekonomski veoma značajna gljiva, rasprostranjena je u većini regiona gajenja hrizanteme u svetu. U našoj zemlji prisustvo ove gljive poslednji put zabeleženo je pre dvadesetak godina. Ova vrsta se nalazi na A2 karantinskoj listi štetnih organizama SCG i EPPO. Tokom 2005. godine, na lokalitetu Vinca ustanovljena je intenzivna pojava bele rđe na hrizantemi gajenoj na otvorenom polju. Identifikacija ovog obligatnog patogena obavljena je na osnovu simptoma oboljenja i morfoloških karakteristika gljive. Na licu lista uočavaju se brojne, ulegnute hlorotične pege prečnika 1-2 mm. Na naličju, formiraju se beli brašnasti ispupčeni sorusi, koji po rasporedu odgovaraju hlorotičnim pegama na licu lista. Jako napadnuti listovi počinju da žute, venu i na kraju se potpuno osuše. U okviru belih sorusa sa naličja listova hrizanteme uočeno je prisustvo karakterističnih glatkih, hijalinskih, dvoćelijskih teleutospora, prosečnih dimenzija 41.25 x 15μm Teleutospore su blago ulegnute duž centralne septe, nalaze se na pedicelu, a na vrhu gornje ćelije nalazi se zadebljanje. U vlažnim uslovima, teleutospore lako klijaju in situ. U uslovima veštačke zaraze, karakteristični simptomi na reznicama hrizanteme razvili su se oko 15 dana po inokulaciji. U cilju sprečavanja pojave i širenja P. horiana na nekom području, preporučuju se karantinske i ostale fitosanitarne mere koje obuhvataju: stalni pregled sadnog materijala, potpuno ili delimično uništavanje useva u kojem dođe do pojave zaraze, kao i tretiranje okolnih osetljivih biljaka po "protokolu tri tretiranja". Doslednom primenom odgovarajućih mera može se postići potpuna eradikacija ovog patogena sa nekog područja. Prilikom hemijske kontrole prouzrokovača bele rđe hrizanteme, treba posvetiti pažnju odabiru i smeni fungicida sa različitim mehanizmima delovanja, zbog razvijanja rezistentnosti ili smanjene osetljivosti populacije gljive na pojedina jedinjenja. U ovom radu potvrđena je ponovna pojava gljive Puccinia horiana, prouzrokovača bele rđe hrizanteme u našoj zemlji, i date su preporuke o neophodnim koracima koje treba preduzeti da se ne bi ugrozila domaća proizvodnja hrizanteme.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Puccinio horiana: The cause of chrysanthemum white rust
T1  - Puccinia horiana - prouzrokovač bele rđe hrizanteme
EP  - 54
IS  - 1
SP  - 49
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1221
ER  - 

@article{
author = "Bulajić, Aleksandra and Krstić, Branka and Dukić, Nataša and Duduk, Bojan",
year = "2006",
abstract = "Puccinia horiana, the cause of chrysanthemum white rust, is economically a very important fungus, which is widely distributed in almost all chrysanthemum growing regions. The presence of P. horiana in our country was last reported nearly 20 years ago. This pathogen is a quarantine pest, included on the SCG A2 list as well as the EPPO A2 list. In 2005 an intensive appearance of chrysanthemum white rust was recorded in the locality of Vinca. Identification of this obligate pathogen was performed by studying disease symptoms and the pathogen's morphological features. On the upper leaf surface numerous pale green to yellow sunken spots, 1-2 mm in diameter on the overage were noticeable. On the corresponding lower leaf surface, a buff of whitish pustules, telia, were found. Severely attacked leaves become yellowish, wilt and gradually dry up completely. In the whitish telia, formed on the lower leaf surface, the characteristic smooth, hyaline 2-celled teliospores with average dimensions of 41.25 x 15μm were established. Teiispores are on pedicel and slightly constricted along septae. The upper cell is thicker at the apex. Teliospores easily germinate in situ under moist conditions. About 15 days after artificial inoculation, chrysanthemum cut­tings reacted with characteristic symptoms. In order to prevent appearance or further spreading of P. horiana in certain regions, quarantine and other phytosanitary measures are recommended. These measures include: routine nursery inspections, in case of a disease outbreak, crop within the facility must be destroyed completely or partially, while surrounding susceptible plants are to be protected according to "three treatment protocol". Following strict appropriate measures, complete eradication of P. horiana from a certain region can be accomplished. Chemical treatment schedule should include fungicides with different modes of action and their rotation, in order to avoid development of pathogen resistance. In this investigation, reappearance of P. horiana, the causal agent of chrysanthemum white rust, was confirmed in the locality of Vinca, in open field chrysanthemum production. Control measure recommendations were summarized as well, in order to protect chrysanthemum production in our country. ., Puccinia horiana, prouzrokovač bele rđe hrizanteme, ekonomski veoma značajna gljiva, rasprostranjena je u većini regiona gajenja hrizanteme u svetu. U našoj zemlji prisustvo ove gljive poslednji put zabeleženo je pre dvadesetak godina. Ova vrsta se nalazi na A2 karantinskoj listi štetnih organizama SCG i EPPO. Tokom 2005. godine, na lokalitetu Vinca ustanovljena je intenzivna pojava bele rđe na hrizantemi gajenoj na otvorenom polju. Identifikacija ovog obligatnog patogena obavljena je na osnovu simptoma oboljenja i morfoloških karakteristika gljive. Na licu lista uočavaju se brojne, ulegnute hlorotične pege prečnika 1-2 mm. Na naličju, formiraju se beli brašnasti ispupčeni sorusi, koji po rasporedu odgovaraju hlorotičnim pegama na licu lista. Jako napadnuti listovi počinju da žute, venu i na kraju se potpuno osuše. U okviru belih sorusa sa naličja listova hrizanteme uočeno je prisustvo karakterističnih glatkih, hijalinskih, dvoćelijskih teleutospora, prosečnih dimenzija 41.25 x 15μm Teleutospore su blago ulegnute duž centralne septe, nalaze se na pedicelu, a na vrhu gornje ćelije nalazi se zadebljanje. U vlažnim uslovima, teleutospore lako klijaju in situ. U uslovima veštačke zaraze, karakteristični simptomi na reznicama hrizanteme razvili su se oko 15 dana po inokulaciji. U cilju sprečavanja pojave i širenja P. horiana na nekom području, preporučuju se karantinske i ostale fitosanitarne mere koje obuhvataju: stalni pregled sadnog materijala, potpuno ili delimično uništavanje useva u kojem dođe do pojave zaraze, kao i tretiranje okolnih osetljivih biljaka po "protokolu tri tretiranja". Doslednom primenom odgovarajućih mera može se postići potpuna eradikacija ovog patogena sa nekog područja. Prilikom hemijske kontrole prouzrokovača bele rđe hrizanteme, treba posvetiti pažnju odabiru i smeni fungicida sa različitim mehanizmima delovanja, zbog razvijanja rezistentnosti ili smanjene osetljivosti populacije gljive na pojedina jedinjenja. U ovom radu potvrđena je ponovna pojava gljive Puccinia horiana, prouzrokovača bele rđe hrizanteme u našoj zemlji, i date su preporuke o neophodnim koracima koje treba preduzeti da se ne bi ugrozila domaća proizvodnja hrizanteme.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Puccinio horiana: The cause of chrysanthemum white rust, Puccinia horiana - prouzrokovač bele rđe hrizanteme",
pages = "54-49",
number = "1",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1221"
}

Bulajić, A., Krstić, B., Dukić, N.,& Duduk, B.. (2006). Puccinio horiana: The cause of chrysanthemum white rust. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(1), 49-54.
https://hdl.handle.net/21.15107/rcub_agrospace_1221

Bulajić A, Krstić B, Dukić N, Duduk B. Puccinio horiana: The cause of chrysanthemum white rust. in Pesticidi i fitomedicina. 2006;21(1):49-54.
https://hdl.handle.net/21.15107/rcub_agrospace_1221 .

Bulajić, Aleksandra, Krstić, Branka, Dukić, Nataša, Duduk, Bojan, "Puccinio horiana: The cause of chrysanthemum white rust" in Pesticidi i fitomedicina, 21, no. 1 (2006):49-54,
https://hdl.handle.net/21.15107/rcub_agrospace_1221 .

TY  - JOUR
AU  - Duduk, Bojan
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Bertaccini, Assunta
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1199
AB  - During a survey on ornamental crops growing in open field, chrysanthemum plants showing proliferation, virescence and stunting were observed. To verify possible phytoplasma association with described symptoms, polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses on phytoplasma 16Sr DNA were carried out. Phytoplasmas belonging to 16SrXII-A ribosomal subgroup (stolbur) were identified in all samples tested from symptomatic plants. This first report of 16SrXII-A stolbur phytoplasmas in chrysanthemum with flower proliferation and stunting symptoms allow to distinguish this from already known chrysanthemum yellows associated with phytoplasmas belonging to aster yellows 16Srl-B ribosomal group, "Candidatus Phytoplasma asteris".
AB  - Tokom pregleda otvorenog polja sa ukrasnim biljkama na lokalitetu Vinča, Beograd, primećene su biljke hrizanteme sa simptomima fitoplazmoza. Primećeni simptomi su obuhvatali proliferaciju cvetova, virescenciju i kržljavost. Radi provere moguće zaraze fitoplazmama, simptomatične biljke hrizanteme analizirane su molekularnim tehnikama, umetnutom lančanom reakcijom polimeraze (nested PCR) praćenom analizom polimorfizma dužine restrikcionih fragmenata (RFLP) na 16Sr DNA. U svim testiranim uzorcima uzetim sa simtomatičnih biljaka hrizanteme identifikovane su fitoplazme iz 16SrXII-A ribozomalne podgrupe. Prvi nalaz 16SrXII-A stolbur fitoplazmi u biljkama hrizanteme sa simptomima proliferacije cvetova, virescencije i kržljavosti razlikuje ovu fitoplazmozu od već poznate "chrysanthemum yellows" kojaje povezana sa fitoplazmama koje pripadaju aster yellows 16Srl-B ribozomalnoj podgrupi, "Candidatus Phytoplasma asteris". .
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Stolbur phytoplasmas infecting chrysanthemum plants in Serbia
T1  - Stolbur fitoplazme na hrizantemi u Srbiji
EP  - 112
IS  - 2
SP  - 107
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1199
ER  - 

@article{
author = "Duduk, Bojan and Dukić, Nataša and Bulajić, Aleksandra and Krstić, Branka and Bertaccini, Assunta",
year = "2006",
abstract = "During a survey on ornamental crops growing in open field, chrysanthemum plants showing proliferation, virescence and stunting were observed. To verify possible phytoplasma association with described symptoms, polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses on phytoplasma 16Sr DNA were carried out. Phytoplasmas belonging to 16SrXII-A ribosomal subgroup (stolbur) were identified in all samples tested from symptomatic plants. This first report of 16SrXII-A stolbur phytoplasmas in chrysanthemum with flower proliferation and stunting symptoms allow to distinguish this from already known chrysanthemum yellows associated with phytoplasmas belonging to aster yellows 16Srl-B ribosomal group, "Candidatus Phytoplasma asteris"., Tokom pregleda otvorenog polja sa ukrasnim biljkama na lokalitetu Vinča, Beograd, primećene su biljke hrizanteme sa simptomima fitoplazmoza. Primećeni simptomi su obuhvatali proliferaciju cvetova, virescenciju i kržljavost. Radi provere moguće zaraze fitoplazmama, simptomatične biljke hrizanteme analizirane su molekularnim tehnikama, umetnutom lančanom reakcijom polimeraze (nested PCR) praćenom analizom polimorfizma dužine restrikcionih fragmenata (RFLP) na 16Sr DNA. U svim testiranim uzorcima uzetim sa simtomatičnih biljaka hrizanteme identifikovane su fitoplazme iz 16SrXII-A ribozomalne podgrupe. Prvi nalaz 16SrXII-A stolbur fitoplazmi u biljkama hrizanteme sa simptomima proliferacije cvetova, virescencije i kržljavosti razlikuje ovu fitoplazmozu od već poznate "chrysanthemum yellows" kojaje povezana sa fitoplazmama koje pripadaju aster yellows 16Srl-B ribozomalnoj podgrupi, "Candidatus Phytoplasma asteris". .",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Stolbur phytoplasmas infecting chrysanthemum plants in Serbia, Stolbur fitoplazme na hrizantemi u Srbiji",
pages = "112-107",
number = "2",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1199"
}

Duduk, B., Dukić, N., Bulajić, A., Krstić, B.,& Bertaccini, A.. (2006). Stolbur phytoplasmas infecting chrysanthemum plants in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(2), 107-112.
https://hdl.handle.net/21.15107/rcub_agrospace_1199

Duduk B, Dukić N, Bulajić A, Krstić B, Bertaccini A. Stolbur phytoplasmas infecting chrysanthemum plants in Serbia. in Pesticidi i fitomedicina. 2006;21(2):107-112.
https://hdl.handle.net/21.15107/rcub_agrospace_1199 .

Duduk, Bojan, Dukić, Nataša, Bulajić, Aleksandra, Krstić, Branka, Bertaccini, Assunta, "Stolbur phytoplasmas infecting chrysanthemum plants in Serbia" in Pesticidi i fitomedicina, 21, no. 2 (2006):107-112,
https://hdl.handle.net/21.15107/rcub_agrospace_1199 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Berenji, Janoš
AU  - Djekić, Ivana
AU  - Duduk, Bojan
AU  - Krstić, Branka
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1184
AB  - Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ring-spot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV). The occurrence and distribution of tobacco viruses were investigated for 4 years (20022005). During this period many different tobacco growing localities in Vojvodina and central Serbia were monitored and samples showing virus symptoms were collected. The collected samples were tested by DAS ELISA using polyclonal antisera, specific for the detection of PVY, TSWV, TMV, CMV, AMV and TRSV. The results obtained for the tobacco virus distribution during these four years of investigation proved the presence of four economically important viruses in our country, whose frequencies varied from year to year. In 2002, 2003 and 2004, the most frequent was TSWV (86.84%; 79% and 49.56%, respectively), while in 2005 PVY was prevalent (56.16%). All viruses detected in the samples tested were present in single or mixed infections. A correlation was established between the field symptoms on tobacco and the virus causal agents. The results obtained showed that TSWV and PVY were the most important tobacco viruses in our country, so that further research of tobacco virus diseases should be directed towards their more detailed characterization.
AB  - Prinos i kvalitet duvana mogu da budu ugroženi usled zaraze velikim brojem fitopatogenih virusa. Duvan je prirodni domaćin preko 20 virusa od kojih su najznačajniji i ekonomski najštetniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), virus graviranosti duvana (Tobacco each virus, TEV) i virus šarenila nerava duvana (Tobacco vein mottling virus, TVMV). Prisustvo i rasprostranjenost virusnih zaraza duvana su ispitivani tokom četiri godine, od 2002. do 2005. Pregled i sakupljanje uzoraka sa simptomima obavljeno je u više različitih lokaliteta gajenja duvana u Vojvodini i centralnoj Srbiji. Sakupljeni uzorci su testirani DASELISA metodom na prisustvo PVY, TSWV, TMV, CMV, AMV i TRSV. Dobijeni rezultati ispitivanja ukazuju na prisustvo četiri ekonomski značajna virusa duvana u našoj zemlji i to: TSWV, PVY, TMV i CMV, čija je učestalost po pojedinim godinama i lokalitetima bila različita. Tokom 2002, 2003. i 2004. godine najčešće detektovan virus bio je TSWV (86,84%; 70%, odnosno 49,56%), dok je 2005. godine prevalentan virus bio PVY (56,16%). Svi virusi čije je prisustvo utvrđeno u ispitivanim uzorcima nalazili su se u pojedinačnim ili mešanim infekcijama. Bez obzira na izraženu varijabilnost u ispoljenim simptomima virusnih zaraza na duvanu, ustanovljena je zavisnost tipa ispoljenih simptoma od virusa prouzrokovača. Sprovedena ispitivanja pokazala su da su TSWV i PVY najznačajniji virusi duvana u našoj zemlji, tako da buduća ispitivanja viroza duvana treba da budu usmerena ka detaljnijoj karakterizaciji ovih ekonomski štetnih virusa.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and distribution of tobacco viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa duvana u Srbiji
EP  - 214
IS  - 3
SP  - 205
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1184
ER  - 

@article{
author = "Dukić, Nataša and Bulajić, Aleksandra and Berenji, Janoš and Djekić, Ivana and Duduk, Bojan and Krstić, Branka",
year = "2006",
abstract = "Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ring-spot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV). The occurrence and distribution of tobacco viruses were investigated for 4 years (20022005). During this period many different tobacco growing localities in Vojvodina and central Serbia were monitored and samples showing virus symptoms were collected. The collected samples were tested by DAS ELISA using polyclonal antisera, specific for the detection of PVY, TSWV, TMV, CMV, AMV and TRSV. The results obtained for the tobacco virus distribution during these four years of investigation proved the presence of four economically important viruses in our country, whose frequencies varied from year to year. In 2002, 2003 and 2004, the most frequent was TSWV (86.84%; 79% and 49.56%, respectively), while in 2005 PVY was prevalent (56.16%). All viruses detected in the samples tested were present in single or mixed infections. A correlation was established between the field symptoms on tobacco and the virus causal agents. The results obtained showed that TSWV and PVY were the most important tobacco viruses in our country, so that further research of tobacco virus diseases should be directed towards their more detailed characterization., Prinos i kvalitet duvana mogu da budu ugroženi usled zaraze velikim brojem fitopatogenih virusa. Duvan je prirodni domaćin preko 20 virusa od kojih su najznačajniji i ekonomski najštetniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), virus graviranosti duvana (Tobacco each virus, TEV) i virus šarenila nerava duvana (Tobacco vein mottling virus, TVMV). Prisustvo i rasprostranjenost virusnih zaraza duvana su ispitivani tokom četiri godine, od 2002. do 2005. Pregled i sakupljanje uzoraka sa simptomima obavljeno je u više različitih lokaliteta gajenja duvana u Vojvodini i centralnoj Srbiji. Sakupljeni uzorci su testirani DASELISA metodom na prisustvo PVY, TSWV, TMV, CMV, AMV i TRSV. Dobijeni rezultati ispitivanja ukazuju na prisustvo četiri ekonomski značajna virusa duvana u našoj zemlji i to: TSWV, PVY, TMV i CMV, čija je učestalost po pojedinim godinama i lokalitetima bila različita. Tokom 2002, 2003. i 2004. godine najčešće detektovan virus bio je TSWV (86,84%; 70%, odnosno 49,56%), dok je 2005. godine prevalentan virus bio PVY (56,16%). Svi virusi čije je prisustvo utvrđeno u ispitivanim uzorcima nalazili su se u pojedinačnim ili mešanim infekcijama. Bez obzira na izraženu varijabilnost u ispoljenim simptomima virusnih zaraza na duvanu, ustanovljena je zavisnost tipa ispoljenih simptoma od virusa prouzrokovača. Sprovedena ispitivanja pokazala su da su TSWV i PVY najznačajniji virusi duvana u našoj zemlji, tako da buduća ispitivanja viroza duvana treba da budu usmerena ka detaljnijoj karakterizaciji ovih ekonomski štetnih virusa.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and distribution of tobacco viruses in Serbia, Prisustvo i rasprostranjenost virusa duvana u Srbiji",
pages = "214-205",
number = "3",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1184"
}

Dukić, N., Bulajić, A., Berenji, J., Djekić, I., Duduk, B.,& Krstić, B.. (2006). Presence and distribution of tobacco viruses in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(3), 205-214.
https://hdl.handle.net/21.15107/rcub_agrospace_1184

Dukić N, Bulajić A, Berenji J, Djekić I, Duduk B, Krstić B. Presence and distribution of tobacco viruses in Serbia. in Pesticidi i fitomedicina. 2006;21(3):205-214.
https://hdl.handle.net/21.15107/rcub_agrospace_1184 .

Dukić, Nataša, Bulajić, Aleksandra, Berenji, Janoš, Djekić, Ivana, Duduk, Bojan, Krstić, Branka, "Presence and distribution of tobacco viruses in Serbia" in Pesticidi i fitomedicina, 21, no. 3 (2006):205-214,
https://hdl.handle.net/21.15107/rcub_agrospace_1184 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Berenji, Janoš
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1140
AB  - Tobacco can be infected with a large number of viruses and some of them are of great economic importance. Since the control of virus diseases is very complicated it makes the significance of tobacco viruses even greater. Although there are no direct chemical measures against viruses and there are no possibilities to cure diseased plants, it is still possible to control the diseases efficiently. In this respect good knowledge of biology and epidemiology of viruses is essential. There is a whole set of measures mostly preventive, in connection with the primary and secondary means of virus spread. That is why it is important to know basic virus characteristics, inoculum sources and the ways of transmission of viruses in nature described in this paper. Even though the choice of control strategy depends on the specific virus, there are some general principles shown in this article. The paper specially focuses on tobacco mosaic virus as the most important virus disease of tobacco in our environment. All sanitary measures which have to be strictly conducted in transplant and crop production have been specified. Significance of the use of resistant cultivars and efficient crop rotation were stressed. The possibilities of biological control measures as well as development of molecular strategies which result in transgenic tobacco plants resistant to tobacco mosaic virus, have also been demonstrated.
AB  - Duvan je domaćin velikog broja virusa. Neki od njih su prouzrokovači značajnih ekonomskih šteta. Zbog veoma složene kontrole virusnih oboljenja značaj virusa duvana je još veći. Iako protiv virusa nema direktnih hemijskih mera borbe i nema mogućnosti izlečenja zaraženih biljaka, ipak se može i mora sprovoditi kontrola oboljenja. Postoji čitav niz mera, pre svega preventivnih, koje su vezane za načine primarnog i sekundarnog širenja virusa. Zbog toga je neophodno poznavanje osnovnih karakteristika virusa izvora inokuluma i načina širenja virusa u prirodi. Mada izbor određene strategije kontrole zavisi od određenog virusa, postoje i opšti principi koji su izneti u ovom radu. U tom kontekstu je, u ovom radu, izneto šta je sve potrebno znati o virusu mozaika duvana kako bi se smanjila njegova štetnost u proizvodnji duvana. Pored toga, navedene su i one sanitarne mere kojih se treba strogo pridržavati i u proizvodnji rasada i pri gajenju useva duvana na otvorenom polju. Osim toga, ukazano je na značaj korišćenja otpornih sorti i primene plodoreda. Dat je pregled mogućnosti kontrole oboljenja koje pružaju biološke mere borbe, kao i razvijanje molekularnih strategija stvaranjem transgenih biljaka otpornih na virus mozaika duvana.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - General principles of tobacco virus diseases management with special reference to tobacco mosaic virus
T1  - Opšti principi kontrole virusnih oboljenja duvana sa posebnim osvrtom na virus mozaika duvana
EP  - 412
IS  - 1
SP  - 401
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1140
ER  - 

@article{
author = "Krstić, Branka and Vico, Ivana and Berenji, Janoš and Dukić, Nataša and Bulajić, Aleksandra",
year = "2006",
abstract = "Tobacco can be infected with a large number of viruses and some of them are of great economic importance. Since the control of virus diseases is very complicated it makes the significance of tobacco viruses even greater. Although there are no direct chemical measures against viruses and there are no possibilities to cure diseased plants, it is still possible to control the diseases efficiently. In this respect good knowledge of biology and epidemiology of viruses is essential. There is a whole set of measures mostly preventive, in connection with the primary and secondary means of virus spread. That is why it is important to know basic virus characteristics, inoculum sources and the ways of transmission of viruses in nature described in this paper. Even though the choice of control strategy depends on the specific virus, there are some general principles shown in this article. The paper specially focuses on tobacco mosaic virus as the most important virus disease of tobacco in our environment. All sanitary measures which have to be strictly conducted in transplant and crop production have been specified. Significance of the use of resistant cultivars and efficient crop rotation were stressed. The possibilities of biological control measures as well as development of molecular strategies which result in transgenic tobacco plants resistant to tobacco mosaic virus, have also been demonstrated., Duvan je domaćin velikog broja virusa. Neki od njih su prouzrokovači značajnih ekonomskih šteta. Zbog veoma složene kontrole virusnih oboljenja značaj virusa duvana je još veći. Iako protiv virusa nema direktnih hemijskih mera borbe i nema mogućnosti izlečenja zaraženih biljaka, ipak se može i mora sprovoditi kontrola oboljenja. Postoji čitav niz mera, pre svega preventivnih, koje su vezane za načine primarnog i sekundarnog širenja virusa. Zbog toga je neophodno poznavanje osnovnih karakteristika virusa izvora inokuluma i načina širenja virusa u prirodi. Mada izbor određene strategije kontrole zavisi od određenog virusa, postoje i opšti principi koji su izneti u ovom radu. U tom kontekstu je, u ovom radu, izneto šta je sve potrebno znati o virusu mozaika duvana kako bi se smanjila njegova štetnost u proizvodnji duvana. Pored toga, navedene su i one sanitarne mere kojih se treba strogo pridržavati i u proizvodnji rasada i pri gajenju useva duvana na otvorenom polju. Osim toga, ukazano je na značaj korišćenja otpornih sorti i primene plodoreda. Dat je pregled mogućnosti kontrole oboljenja koje pružaju biološke mere borbe, kao i razvijanje molekularnih strategija stvaranjem transgenih biljaka otpornih na virus mozaika duvana.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "General principles of tobacco virus diseases management with special reference to tobacco mosaic virus, Opšti principi kontrole virusnih oboljenja duvana sa posebnim osvrtom na virus mozaika duvana",
pages = "412-401",
number = "1",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1140"
}

Krstić, B., Vico, I., Berenji, J., Dukić, N.,& Bulajić, A.. (2006). General principles of tobacco virus diseases management with special reference to tobacco mosaic virus. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 42(1), 401-412.
https://hdl.handle.net/21.15107/rcub_agrospace_1140

Krstić B, Vico I, Berenji J, Dukić N, Bulajić A. General principles of tobacco virus diseases management with special reference to tobacco mosaic virus. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2006;42(1):401-412.
https://hdl.handle.net/21.15107/rcub_agrospace_1140 .

Krstić, Branka, Vico, Ivana, Berenji, Janoš, Dukić, Nataša, Bulajić, Aleksandra, "General principles of tobacco virus diseases management with special reference to tobacco mosaic virus" in Zbornik radova Instituta za ratarstvo i povrtarstvo, 42, no. 1 (2006):401-412,
https://hdl.handle.net/21.15107/rcub_agrospace_1140 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Krstić, Branka
AU  - Berenji, Janoš
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1169
AB  - Cucurbits are very important crops in many countries. The most significant cucurbits diseases are those caused by viruses. Concerning pumpkin virus diseases, three viruses were detected in Serbia after 2000: Cucumber mosaic virus (CMV), Watermelon mosaic virus 2 (WMV-2) and Zucchini yellow mosaic virus (ZYMV). Viral infections symptoms on pumpkins are very severe and variable. Disease appearance and spread depends on the causal virus. There are different control measures for viral diseases, but most of them are based on prevention.
AB  - Vrste familije Cucurbitaceae predstavljaju značajnu grupu gajenih biljaka u mnogim zemljama sveta. U proizvodnji, prvo mesto po značaju zauzimaju bolesti virusne prirode. Sprovedenim ispitivanjima od 2000. godine utvrđeno je prisustvo tri virusa na običnoj tikvi (virus mozaika krastavca - Cucumber mosaic virus, CMV; virus mozaika lubenice 2 - Watermelon mosaic virus 2, WMV-2; virus žutog mozaika cukinija - Zucchini yellow mosaic virus, ZYMV). Simptomi virusnih zaraza mogu biti veoma izraženi i raznovrsni. Pojava i širenje bolesti uslovljena je virusnim prouzrokovačem. Postoje različite mere zaštite od virusnih bolesti, koje su usmerene na preventivu i prekidanje epidemiološkog lanca bolesti.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Cucurbit viral diseases
T1  - Virusna oboljenja tikve
EP  - 235
IS  - 3
SP  - 230
VL  - 34
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1169
ER  - 

@article{
author = "Dukić, Nataša and Krstić, Branka and Berenji, Janoš",
year = "2006",
abstract = "Cucurbits are very important crops in many countries. The most significant cucurbits diseases are those caused by viruses. Concerning pumpkin virus diseases, three viruses were detected in Serbia after 2000: Cucumber mosaic virus (CMV), Watermelon mosaic virus 2 (WMV-2) and Zucchini yellow mosaic virus (ZYMV). Viral infections symptoms on pumpkins are very severe and variable. Disease appearance and spread depends on the causal virus. There are different control measures for viral diseases, but most of them are based on prevention., Vrste familije Cucurbitaceae predstavljaju značajnu grupu gajenih biljaka u mnogim zemljama sveta. U proizvodnji, prvo mesto po značaju zauzimaju bolesti virusne prirode. Sprovedenim ispitivanjima od 2000. godine utvrđeno je prisustvo tri virusa na običnoj tikvi (virus mozaika krastavca - Cucumber mosaic virus, CMV; virus mozaika lubenice 2 - Watermelon mosaic virus 2, WMV-2; virus žutog mozaika cukinija - Zucchini yellow mosaic virus, ZYMV). Simptomi virusnih zaraza mogu biti veoma izraženi i raznovrsni. Pojava i širenje bolesti uslovljena je virusnim prouzrokovačem. Postoje različite mere zaštite od virusnih bolesti, koje su usmerene na preventivu i prekidanje epidemiološkog lanca bolesti.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Cucurbit viral diseases, Virusna oboljenja tikve",
pages = "235-230",
number = "3",
volume = "34",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1169"
}

Dukić, N., Krstić, B.,& Berenji, J.. (2006). Cucurbit viral diseases. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 34(3), 230-235.
https://hdl.handle.net/21.15107/rcub_agrospace_1169

Dukić N, Krstić B, Berenji J. Cucurbit viral diseases. in Biljni lekar. 2006;34(3):230-235.
https://hdl.handle.net/21.15107/rcub_agrospace_1169 .

Dukić, Nataša, Krstić, Branka, Berenji, Janoš, "Cucurbit viral diseases" in Biljni lekar, 34, no. 3 (2006):230-235,
https://hdl.handle.net/21.15107/rcub_agrospace_1169 .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Vico, Ivana
AU  - Krstić, Branka
AU  - Dukić, Nataša
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/938
AB  - Cultivated lilies (Lilium spp., fam Liliaceae) represent one of the economically very important groups of ornamental plants. Some of the hybrids are among the top-selling categories of cut and pot flowers. In almost all lily--growing regions, two fungus species from the genus Botrytis, B. elliptica and B. cinerea, are known to be limiting factors in production, regularly causing considerable losses. Both species are the causal agents of leaf spot and decay, known as fire of lilies. B. elliptica has a narrow host range, restricted to Lilium spp., while the host range of B. cinerea is wide and includes almost all dicotyledonous plants. Although Botrytis sp. have several anamorphs as well as the teliomorph, their sclerotia, formed on plant debris, are the most important for conservation and over wintering. In the spring, fructifying sclerotia give conidia and rarely ascospores, and both provide an initial source of inoculum. Control measures against Botrytis sp. in lily production are very complicated and expensive. Regardless of frequency and quality of treatment, or choice of fungicides, chemical measures alone are insufficient for solving the problem of fire of lilies. It is necessary to employ all available phytosanitary measures in order to reduce pathogen populations or avoid conditions leading to infection.
AB  - Gajeni ljiljani (Lilium spp., fam. Liliaceae) predstavljaju jednu od ekonomski najznačajnijih grupacija ukrasnih biljaka, čiji pojedini hibridi spadaju u najprodavanije cveće u kategorijama, kako saksijskog tako i sečenog cveća. U većini regiona gajenja ljiljana, ograničavajući faktor u proizvodnji predstavljaju dve gljive iz roda Botrytis; B. elliptica i B. cinerea koje redovno izazivaju ekonomski značajne gubitke. Ove gljive su prouzrokovači pegavosti i sušenja lista, bolesti koja je poznata i pod imenom "vatra ljiljana". B. elliptica je usko specijalizovana vrsta i po krugu domaćina ograničena na ljiljan, dok je B. cinerea polifagna vrsta koja napada većinu dikotiledonih biljaka. lako se gljive iz roda Botrytis u prirodi javljaju u više anamorfnih i u teleomorfnom stadijumu, njihove sklerocije koje se formiraju na biljnim ostacima predstavljaju najvažniji vid održavanja. Na prezimelim sklerocijama B. elliptica u proleće se uglavnom formiraju konidije koje predstavljaju primarni, inicijalni izvor inokuluma. Na prezimelim sklerocijama retko se formiraju i askospore. U proizvodnji ljiljana kontrola gljiva iz roda Botrytis vrlo je komplikovana i skupa. Bez obzira na učestalost i kvalitet tretiranja i odabir jedinjenja za primenu, hemijske mere borbe same za sebe ne mogu na zadovoljavajući način da rese problem sušenja lista ljiljana. Neophodno je primeniti i sve druge fitosanitarne mere koje će redukovati populaciju patogena ili onemogućiti uslove za ostvarenje zaraze.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Botrytis species on Lilies and possibilities for their control
T1  - Botrytis vrste na ljiljanu i mogućnost zaštite
EP  - 234
IS  - 4
SP  - 229
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_938
ER  - 

@article{
author = "Bulajić, Aleksandra and Vico, Ivana and Krstić, Branka and Dukić, Nataša",
year = "2005",
abstract = "Cultivated lilies (Lilium spp., fam Liliaceae) represent one of the economically very important groups of ornamental plants. Some of the hybrids are among the top-selling categories of cut and pot flowers. In almost all lily--growing regions, two fungus species from the genus Botrytis, B. elliptica and B. cinerea, are known to be limiting factors in production, regularly causing considerable losses. Both species are the causal agents of leaf spot and decay, known as fire of lilies. B. elliptica has a narrow host range, restricted to Lilium spp., while the host range of B. cinerea is wide and includes almost all dicotyledonous plants. Although Botrytis sp. have several anamorphs as well as the teliomorph, their sclerotia, formed on plant debris, are the most important for conservation and over wintering. In the spring, fructifying sclerotia give conidia and rarely ascospores, and both provide an initial source of inoculum. Control measures against Botrytis sp. in lily production are very complicated and expensive. Regardless of frequency and quality of treatment, or choice of fungicides, chemical measures alone are insufficient for solving the problem of fire of lilies. It is necessary to employ all available phytosanitary measures in order to reduce pathogen populations or avoid conditions leading to infection., Gajeni ljiljani (Lilium spp., fam. Liliaceae) predstavljaju jednu od ekonomski najznačajnijih grupacija ukrasnih biljaka, čiji pojedini hibridi spadaju u najprodavanije cveće u kategorijama, kako saksijskog tako i sečenog cveća. U većini regiona gajenja ljiljana, ograničavajući faktor u proizvodnji predstavljaju dve gljive iz roda Botrytis; B. elliptica i B. cinerea koje redovno izazivaju ekonomski značajne gubitke. Ove gljive su prouzrokovači pegavosti i sušenja lista, bolesti koja je poznata i pod imenom "vatra ljiljana". B. elliptica je usko specijalizovana vrsta i po krugu domaćina ograničena na ljiljan, dok je B. cinerea polifagna vrsta koja napada većinu dikotiledonih biljaka. lako se gljive iz roda Botrytis u prirodi javljaju u više anamorfnih i u teleomorfnom stadijumu, njihove sklerocije koje se formiraju na biljnim ostacima predstavljaju najvažniji vid održavanja. Na prezimelim sklerocijama B. elliptica u proleće se uglavnom formiraju konidije koje predstavljaju primarni, inicijalni izvor inokuluma. Na prezimelim sklerocijama retko se formiraju i askospore. U proizvodnji ljiljana kontrola gljiva iz roda Botrytis vrlo je komplikovana i skupa. Bez obzira na učestalost i kvalitet tretiranja i odabir jedinjenja za primenu, hemijske mere borbe same za sebe ne mogu na zadovoljavajući način da rese problem sušenja lista ljiljana. Neophodno je primeniti i sve druge fitosanitarne mere koje će redukovati populaciju patogena ili onemogućiti uslove za ostvarenje zaraze.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Botrytis species on Lilies and possibilities for their control, Botrytis vrste na ljiljanu i mogućnost zaštite",
pages = "234-229",
number = "4",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_938"
}

Bulajić, A., Vico, I., Krstić, B.,& Dukić, N.. (2005). Botrytis species on Lilies and possibilities for their control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 20(4), 229-234.
https://hdl.handle.net/21.15107/rcub_agrospace_938

Bulajić A, Vico I, Krstić B, Dukić N. Botrytis species on Lilies and possibilities for their control. in Pesticidi i fitomedicina. 2005;20(4):229-234.
https://hdl.handle.net/21.15107/rcub_agrospace_938 .

Bulajić, Aleksandra, Vico, Ivana, Krstić, Branka, Dukić, Nataša, "Botrytis species on Lilies and possibilities for their control" in Pesticidi i fitomedicina, 20, no. 4 (2005):229-234,
https://hdl.handle.net/21.15107/rcub_agrospace_938 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Duduk, Bojan
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/937
AB  - Pepino mosaic virus (PepMV) was initially described in 1974 as the causal agent of a viral disease of pepino (Solarium muricatum) in Peru. For a long time there were no other reports of its occurrence. Interest in the virus revived in late 1990s, after it was found in protected tomato crops in the Netherlands and then in other European countries. PepMV is now included on the EPPO Alert List. The virus belongs to the Potexvirus group characterized as highly infectious and persistent. PepMV is a virus easily spread mechanically by contaminated tools, shoes, clothing, hands, and plant-to-plant con- tact. Although symptoms seem related to environmental! conditions and the cultivars, damage is always high, with unmarketable fruits. PepMV is difficult to manage. It is important to prevent the introduction of the disease by using virus-free seeds and transplants. To minimize introduction, spread, and carryover of PepMV, strict hygiene at all stages during crop production are essential.
AB  - Prvi podaci o Pepino mosaic virusu (PepMV) potiču iz sedamdesetih godina, a veliku pažnju ovaj virus počeo je da privlači tek krajem devedesetih, kada je ustanovljeno da može da ugrozi proizvodnju paradajza u zatvorenom prostoru. Virus se nalazi na EPPO Alert listi, prisutan je u mnogim zemljama Evrope, ali kod nas nema podataka o njegovom prisustvu. PepMV je visokoinfektivan, veoma postojan i lako se širi mehanički, kontaktom biljaka, kontaminiranim oruđem, odećom ili rukama, lako ispoljavanje simptoma zavisi od uslova spoljašnje sredine i reakcije sorte paradajza štete su uvek velike i plodovi nemaju tržišnu vrednost. Kontrola ovog virusa je vrlo teška. Najvažnija mera kontrole je sprečavanje pojave bolesti upotrebom zdravog semena i rasada. Da bi se ograničila pojava, prenošenje i širenje ovog virusa neophodno je primeniti stroge higijenske mere u svim fazama tokom proizvodnog ciklusa paradajza u zatvorenom prostoru.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pepino mosaic virus: A new tomato virus in Europe
T1  - Pepino mosaic virus - novi virus paradajza u Evropi
EP  - 228
IS  - 4
SP  - 221
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_937
ER  - 

@article{
author = "Krstić, Branka and Dukić, Nataša and Bulajić, Aleksandra and Duduk, Bojan",
year = "2005",
abstract = "Pepino mosaic virus (PepMV) was initially described in 1974 as the causal agent of a viral disease of pepino (Solarium muricatum) in Peru. For a long time there were no other reports of its occurrence. Interest in the virus revived in late 1990s, after it was found in protected tomato crops in the Netherlands and then in other European countries. PepMV is now included on the EPPO Alert List. The virus belongs to the Potexvirus group characterized as highly infectious and persistent. PepMV is a virus easily spread mechanically by contaminated tools, shoes, clothing, hands, and plant-to-plant con- tact. Although symptoms seem related to environmental! conditions and the cultivars, damage is always high, with unmarketable fruits. PepMV is difficult to manage. It is important to prevent the introduction of the disease by using virus-free seeds and transplants. To minimize introduction, spread, and carryover of PepMV, strict hygiene at all stages during crop production are essential., Prvi podaci o Pepino mosaic virusu (PepMV) potiču iz sedamdesetih godina, a veliku pažnju ovaj virus počeo je da privlači tek krajem devedesetih, kada je ustanovljeno da može da ugrozi proizvodnju paradajza u zatvorenom prostoru. Virus se nalazi na EPPO Alert listi, prisutan je u mnogim zemljama Evrope, ali kod nas nema podataka o njegovom prisustvu. PepMV je visokoinfektivan, veoma postojan i lako se širi mehanički, kontaktom biljaka, kontaminiranim oruđem, odećom ili rukama, lako ispoljavanje simptoma zavisi od uslova spoljašnje sredine i reakcije sorte paradajza štete su uvek velike i plodovi nemaju tržišnu vrednost. Kontrola ovog virusa je vrlo teška. Najvažnija mera kontrole je sprečavanje pojave bolesti upotrebom zdravog semena i rasada. Da bi se ograničila pojava, prenošenje i širenje ovog virusa neophodno je primeniti stroge higijenske mere u svim fazama tokom proizvodnog ciklusa paradajza u zatvorenom prostoru.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pepino mosaic virus: A new tomato virus in Europe, Pepino mosaic virus - novi virus paradajza u Evropi",
pages = "228-221",
number = "4",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_937"
}

Krstić, B., Dukić, N., Bulajić, A.,& Duduk, B.. (2005). Pepino mosaic virus: A new tomato virus in Europe. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 20(4), 221-228.
https://hdl.handle.net/21.15107/rcub_agrospace_937

Krstić B, Dukić N, Bulajić A, Duduk B. Pepino mosaic virus: A new tomato virus in Europe. in Pesticidi i fitomedicina. 2005;20(4):221-228.
https://hdl.handle.net/21.15107/rcub_agrospace_937 .

Krstić, Branka, Dukić, Nataša, Bulajić, Aleksandra, Duduk, Bojan, "Pepino mosaic virus: A new tomato virus in Europe" in Pesticidi i fitomedicina, 20, no. 4 (2005):221-228,
https://hdl.handle.net/21.15107/rcub_agrospace_937 .

TY  - JOUR
AU  - Vico, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Dukić, Nataša
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/936
AB  - Potted chrysanthemum plants (Chrysanthemum Indicum Groep, sin. Chr. x grandiflorum) originating from Mala Pruga, Zemun Polje, exhibited a variety of symptoms including stem and leaf necrosis, as well as bud and flower rot. Necrosis started on stems, which were previously cut in order to provoke flowering. Further on, necrosis spread and covered larger plant areas, and after a period of three weeks killed the plants. From diseased plants fungal isolates were obtained which proved to be pathogenic to healthy stems of chrysanthemum. According to macroscopic and microscopic as well as some biological properties the causal agent of chrysanthemum stem necrosis was identified as multinucleate Rhizoctonia sp. (Rhizoctonia solani).
AB  - Na saksijskim biljkama hrizanteme (Chrysanthemum Indicum Groep, sin. Chr. x grandiflorum) sa lokaliteta Mala Pruga, Zemun Polje, uočeni su simptomi u vidu sužavanja i nekroze stabljika, sušenja pupoljaka, cvetova i listova. Nekrotični proces započeo je na mestima gde su stabljike sečene u cilju provociranja obimnog cvetanja biljaka. Odatle, nekroza se širila da bi posle tri nedelje od pojave prvih simptoma zahvatila čitavu biljku. Iz obolelih stabljika izolovana je gljiva čija je patogenost potvrđena veštačkim inokulacijama zdravih stabljika hrizanteme. Na osnovu morfoloških (makroskopskih i mikroskopskih) i bioloških odlika, fitopatogena gljiva prouzrokovač nekroze hrizanteme, identifikovana je kao višejedarna Rhizoctonia sp.(Rhizoctonia solani).
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Multinucleate Rhizoctonia sp. a pathogen of Chrysanthemum
T1  - Višejedarna Rhizoctonia sp. - patogen hrizanteme
EP  - 188
IS  - 3
SP  - 183
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_936
ER  - 

@article{
author = "Vico, Ivana and Krstić, Branka and Bulajić, Aleksandra and Dukić, Nataša",
year = "2005",
abstract = "Potted chrysanthemum plants (Chrysanthemum Indicum Groep, sin. Chr. x grandiflorum) originating from Mala Pruga, Zemun Polje, exhibited a variety of symptoms including stem and leaf necrosis, as well as bud and flower rot. Necrosis started on stems, which were previously cut in order to provoke flowering. Further on, necrosis spread and covered larger plant areas, and after a period of three weeks killed the plants. From diseased plants fungal isolates were obtained which proved to be pathogenic to healthy stems of chrysanthemum. According to macroscopic and microscopic as well as some biological properties the causal agent of chrysanthemum stem necrosis was identified as multinucleate Rhizoctonia sp. (Rhizoctonia solani)., Na saksijskim biljkama hrizanteme (Chrysanthemum Indicum Groep, sin. Chr. x grandiflorum) sa lokaliteta Mala Pruga, Zemun Polje, uočeni su simptomi u vidu sužavanja i nekroze stabljika, sušenja pupoljaka, cvetova i listova. Nekrotični proces započeo je na mestima gde su stabljike sečene u cilju provociranja obimnog cvetanja biljaka. Odatle, nekroza se širila da bi posle tri nedelje od pojave prvih simptoma zahvatila čitavu biljku. Iz obolelih stabljika izolovana je gljiva čija je patogenost potvrđena veštačkim inokulacijama zdravih stabljika hrizanteme. Na osnovu morfoloških (makroskopskih i mikroskopskih) i bioloških odlika, fitopatogena gljiva prouzrokovač nekroze hrizanteme, identifikovana je kao višejedarna Rhizoctonia sp.(Rhizoctonia solani).",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Multinucleate Rhizoctonia sp. a pathogen of Chrysanthemum, Višejedarna Rhizoctonia sp. - patogen hrizanteme",
pages = "188-183",
number = "3",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_936"
}

Vico, I., Krstić, B., Bulajić, A.,& Dukić, N.. (2005). Multinucleate Rhizoctonia sp. a pathogen of Chrysanthemum. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 20(3), 183-188.
https://hdl.handle.net/21.15107/rcub_agrospace_936

Vico I, Krstić B, Bulajić A, Dukić N. Multinucleate Rhizoctonia sp. a pathogen of Chrysanthemum. in Pesticidi i fitomedicina. 2005;20(3):183-188.
https://hdl.handle.net/21.15107/rcub_agrospace_936 .

Vico, Ivana, Krstić, Branka, Bulajić, Aleksandra, Dukić, Nataša, "Multinucleate Rhizoctonia sp. a pathogen of Chrysanthemum" in Pesticidi i fitomedicina, 20, no. 3 (2005):183-188,
https://hdl.handle.net/21.15107/rcub_agrospace_936 .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Dukić, Nataša
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/906
AB  - Alternaria petroselini (Neergard) Simmons, causal agent of parsley black leaf spot, is economically significant in leaf and root production of parsley (Petroselinum crispum (Mill) Nym). This is a seed- and soil-borne fungus recently described in our country. During the investigation of the presence of A. petroselini in our country, diseased parsley plants were observed and collected from Manđelos, several samples of parsley commercial seed were examined, and isolation was also done from infested soil. Total of seven isolates was obtained and compared, one from diseased leaves, five from parsley seed, and one from soil. After pathogenicity was proven, isolates were identified based on morphological properties, colony appearance, conidia shape and size, catenulation and host range Based on obtained results investigated isolates of different origin identified as Ai-ternana petroselint showed uniform macroscopic and microscopic characteristics as well as the same host range On KDA, ail isolates show uniform characteristics The colonies are very dark gray to blackish, with slightly waved edges and thick, velvety, shiny aerial mycelium Conidia are formed singly in large number, on short, mainly unbranched conidiophores Conidia are smooth, dark brown, dictiosponc, frequently constricted on septae and ranging from 16 25-47 50 urn (29 22 um) x 12 5-27 5 urn (19 62 pm) Tested isolates were pathogenic to parsley, parsnip, celery and coriander but were not pathogenic to carrot, anise, foenicum, onion cabbage, pepper, tomato and cucumber.
AB  - Alternaria petroselini (Neergard) Simmons, prouzrokovač pegavosti i sušenja lista peršuna (Petroselinum crispum (Mill) Nym) izaziva značajne štete u proizvodnji lišća i korena peršuna. Ova gljiva čije je prisustvo nedavno utvrđeno i u našoj zemlji, održava se i širi zaraženim semenom i biljnim ostacima u zemljištu. Ispitujući prisustvo A. petroselini u našoj zemlji vršeni su pregledi zaraženih biljaka na lokalitetu Manđelos, obavljene izolacije gljive iz zemljišta sa istog lokaliteta, i pregledano vise uzoraka komercijalnog semena peršuna. Dobijeno je ukupno sedam izolata, jedan iz zaraženih listova peršuna jedan iz zemljišta i pet sa semena. Nakon provere patogenosti, izolati su determinisani i upoređeni na osnovu morfoloških osobina, izgleda kolonije čistih kultura, oblika, veličine konidija katenulacije, kao i kruga domaćina. Na osnovu dobijenih rezultata, utvrđeno je da izolati A. petroselini različitog porekla ispoljavaju uniformne makroskopske i mikroskopske morfološke osobine i imaju isti krug domaćina. Na KDA podlozi svi izolati formiraju tamnosive, skoro crne kolonije sa blago talasastim ivicama, gustom somotastom sjajnom vazdušnom micelijom Konidije se formiraju u velikom broju, pojedinačno najčešće na kratkim i nerazgranatim konidioforama, glatke su, tamnosmeđe boje, diktiosporne i često ulegnute na poprečnim septama. Dimenzije konidija su 16.25-47.50 μm (29.22 μm) x 12.5-27.5 μm (19.62 μm) Ispitivani izolati ispoljili su patogenost prema peršunu, paštrnaku, celeru i kolijanderu, a nisu patogeni za mrkvu anis morač, crni luk, papriku, paradajz, kupus i krastavac.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Comparative studies of Alternaria petroselini pathogen of parsley
T1  - Uporedna proučavanja izolata Alternaria petroselini patogena peršuna
EP  - 50
IS  - 1
SP  - 43
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_906
ER  - 

@article{
author = "Bulajić, Aleksandra and Krstić, Branka and Vico, Ivana and Dukić, Nataša",
year = "2005",
abstract = "Alternaria petroselini (Neergard) Simmons, causal agent of parsley black leaf spot, is economically significant in leaf and root production of parsley (Petroselinum crispum (Mill) Nym). This is a seed- and soil-borne fungus recently described in our country. During the investigation of the presence of A. petroselini in our country, diseased parsley plants were observed and collected from Manđelos, several samples of parsley commercial seed were examined, and isolation was also done from infested soil. Total of seven isolates was obtained and compared, one from diseased leaves, five from parsley seed, and one from soil. After pathogenicity was proven, isolates were identified based on morphological properties, colony appearance, conidia shape and size, catenulation and host range Based on obtained results investigated isolates of different origin identified as Ai-ternana petroselint showed uniform macroscopic and microscopic characteristics as well as the same host range On KDA, ail isolates show uniform characteristics The colonies are very dark gray to blackish, with slightly waved edges and thick, velvety, shiny aerial mycelium Conidia are formed singly in large number, on short, mainly unbranched conidiophores Conidia are smooth, dark brown, dictiosponc, frequently constricted on septae and ranging from 16 25-47 50 urn (29 22 um) x 12 5-27 5 urn (19 62 pm) Tested isolates were pathogenic to parsley, parsnip, celery and coriander but were not pathogenic to carrot, anise, foenicum, onion cabbage, pepper, tomato and cucumber., Alternaria petroselini (Neergard) Simmons, prouzrokovač pegavosti i sušenja lista peršuna (Petroselinum crispum (Mill) Nym) izaziva značajne štete u proizvodnji lišća i korena peršuna. Ova gljiva čije je prisustvo nedavno utvrđeno i u našoj zemlji, održava se i širi zaraženim semenom i biljnim ostacima u zemljištu. Ispitujući prisustvo A. petroselini u našoj zemlji vršeni su pregledi zaraženih biljaka na lokalitetu Manđelos, obavljene izolacije gljive iz zemljišta sa istog lokaliteta, i pregledano vise uzoraka komercijalnog semena peršuna. Dobijeno je ukupno sedam izolata, jedan iz zaraženih listova peršuna jedan iz zemljišta i pet sa semena. Nakon provere patogenosti, izolati su determinisani i upoređeni na osnovu morfoloških osobina, izgleda kolonije čistih kultura, oblika, veličine konidija katenulacije, kao i kruga domaćina. Na osnovu dobijenih rezultata, utvrđeno je da izolati A. petroselini različitog porekla ispoljavaju uniformne makroskopske i mikroskopske morfološke osobine i imaju isti krug domaćina. Na KDA podlozi svi izolati formiraju tamnosive, skoro crne kolonije sa blago talasastim ivicama, gustom somotastom sjajnom vazdušnom micelijom Konidije se formiraju u velikom broju, pojedinačno najčešće na kratkim i nerazgranatim konidioforama, glatke su, tamnosmeđe boje, diktiosporne i često ulegnute na poprečnim septama. Dimenzije konidija su 16.25-47.50 μm (29.22 μm) x 12.5-27.5 μm (19.62 μm) Ispitivani izolati ispoljili su patogenost prema peršunu, paštrnaku, celeru i kolijanderu, a nisu patogeni za mrkvu anis morač, crni luk, papriku, paradajz, kupus i krastavac.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Comparative studies of Alternaria petroselini pathogen of parsley, Uporedna proučavanja izolata Alternaria petroselini patogena peršuna",
pages = "50-43",
number = "1",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_906"
}

Bulajić, A., Krstić, B., Vico, I.,& Dukić, N.. (2005). Comparative studies of Alternaria petroselini pathogen of parsley. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 20(1), 43-50.
https://hdl.handle.net/21.15107/rcub_agrospace_906

Bulajić A, Krstić B, Vico I, Dukić N. Comparative studies of Alternaria petroselini pathogen of parsley. in Pesticidi i fitomedicina. 2005;20(1):43-50.
https://hdl.handle.net/21.15107/rcub_agrospace_906 .

Bulajić, Aleksandra, Krstić, Branka, Vico, Ivana, Dukić, Nataša, "Comparative studies of Alternaria petroselini pathogen of parsley" in Pesticidi i fitomedicina, 20, no. 1 (2005):43-50,
https://hdl.handle.net/21.15107/rcub_agrospace_906 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Berenji, Janoš
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/859
AB  - Screenings conducted in 2002 indicated the occurrence of viral symptoms on pumpkins in the locations of Bački Petrovac, Futog, Žabalj, Titel, Torda and Veliko Selo. Using two methods of viral identification, biotest and DAS−ELISA serological method, we have confirmed the presence of three viruses, CMV, WMV−2 and ZYMV, in pumpkin samples. ZYMV was the predominant virus of pumpkins in 2002. WMV−2 differed significantly in the frequency of occurrence between years. While it occurred sporadically in 2001, it was the second most frequent virus in 2002. CMV was in the second place in 2001 and in the third place in 2002. The observed variability in the occurrence and spread of viruses among the years is frequent in the case of no persistent viruses of pumpkins. It is also an indication of possible expansion of WMV−2 in Serbia.
AB  - Ispitivanjima sprovedenim tokom 2002. godine zabeležena je pojava virusnih simptoma na običnoj tikvi u lokalitetima Bački Petrovac, Futog, Žabalj Titel, Torda i Veliko Selo. Primenom dve metode identifikacije virusa biotesta i serološke metode, DAS−ELISA, u uzrocima obične tikve utvrđeno je prisustvo tri virusa CMV, WMV−2 i ZYMV. Praćenjem pojave i rasprostranjenosti virusa na običnoj tikvi tokom 2002. godine, potvrđeno je dominantno prisustvo ZYMV. Značajna razlika u učestalosti pojave, u odnosu na ispitivanja ranijih godina, zabeležena je kod WMV−2, koji se u 2001. javljao sporadično, a u 2002. godini bio drugi po zastupljenosti. CMV je tokom 2001. godine bio drugi po zastupljenosti, a tokom 2002. godine treći. Ova varijabilnost u pojavi i rasprostranjenosti virusa u različitim godinama, česta je pojava kod neperzistentnih virusa na gajenim tikvama, ali govori i o mogućoj ekspanziji WMV−2 u našoj zemlji.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia)
T1  - Prisustvo i rasprostranjenost viroza obične tikve (Cucurbita pepo L) u Vojvodini
EP  - 79
IS  - 76-77
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_859
ER  - 

@article{
author = "Dukić, Nataša and Berenji, Janoš and Krstić, Branka and Vico, Ivana and Bulajić, Aleksandra",
year = "2004",
abstract = "Screenings conducted in 2002 indicated the occurrence of viral symptoms on pumpkins in the locations of Bački Petrovac, Futog, Žabalj, Titel, Torda and Veliko Selo. Using two methods of viral identification, biotest and DAS−ELISA serological method, we have confirmed the presence of three viruses, CMV, WMV−2 and ZYMV, in pumpkin samples. ZYMV was the predominant virus of pumpkins in 2002. WMV−2 differed significantly in the frequency of occurrence between years. While it occurred sporadically in 2001, it was the second most frequent virus in 2002. CMV was in the second place in 2001 and in the third place in 2002. The observed variability in the occurrence and spread of viruses among the years is frequent in the case of no persistent viruses of pumpkins. It is also an indication of possible expansion of WMV−2 in Serbia., Ispitivanjima sprovedenim tokom 2002. godine zabeležena je pojava virusnih simptoma na običnoj tikvi u lokalitetima Bački Petrovac, Futog, Žabalj Titel, Torda i Veliko Selo. Primenom dve metode identifikacije virusa biotesta i serološke metode, DAS−ELISA, u uzrocima obične tikve utvrđeno je prisustvo tri virusa CMV, WMV−2 i ZYMV. Praćenjem pojave i rasprostranjenosti virusa na običnoj tikvi tokom 2002. godine, potvrđeno je dominantno prisustvo ZYMV. Značajna razlika u učestalosti pojave, u odnosu na ispitivanja ranijih godina, zabeležena je kod WMV−2, koji se u 2001. javljao sporadično, a u 2002. godini bio drugi po zastupljenosti. CMV je tokom 2001. godine bio drugi po zastupljenosti, a tokom 2002. godine treći. Ova varijabilnost u pojavi i rasprostranjenosti virusa u različitim godinama, česta je pojava kod neperzistentnih virusa na gajenim tikvama, ali govori i o mogućoj ekspanziji WMV−2 u našoj zemlji.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia), Prisustvo i rasprostranjenost viroza obične tikve (Cucurbita pepo L) u Vojvodini",
pages = "79-71",
number = "76-77",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_859"
}

Dukić, N., Berenji, J., Krstić, B., Vico, I.,& Bulajić, A.. (2004). Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia). in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad.(76-77), 71-79.
https://hdl.handle.net/21.15107/rcub_agrospace_859

Dukić N, Berenji J, Krstić B, Vico I, Bulajić A. Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia). in Bilten za hmelj, sirak i lekovito bilje. 2004;(76-77):71-79.
https://hdl.handle.net/21.15107/rcub_agrospace_859 .

Dukić, Nataša, Berenji, Janoš, Krstić, Branka, Vico, Ivana, Bulajić, Aleksandra, "Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia)" in Bilten za hmelj, sirak i lekovito bilje, no. 76-77 (2004):71-79,
https://hdl.handle.net/21.15107/rcub_agrospace_859 .

TY  - CONF
AU  - Uskoković-Marković, Snežana M.
AU  - Todorović, Marija R.
AU  - Mioč, Ubavka B.
AU  - Krstić, Branka
AU  - Dukić, Nataša
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/377
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - New application of 12-tungstophosphoric acid and its magnesium salt against plant viruses
T1  - Nova primena 12-volframfosforne kiseline i njene magnezijumove soli protiv biljnih virusa
EP  - 815
IS  - 4
SP  - 814
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_377
ER  - 

@conference{
author = "Uskoković-Marković, Snežana M. and Todorović, Marija R. and Mioč, Ubavka B. and Krstić, Branka and Dukić, Nataša",
year = "2002",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "New application of 12-tungstophosphoric acid and its magnesium salt against plant viruses, Nova primena 12-volframfosforne kiseline i njene magnezijumove soli protiv biljnih virusa",
pages = "815-814",
number = "4",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_377"
}

Uskoković-Marković, S. M., Todorović, M. R., Mioč, U. B., Krstić, B.,& Dukić, N.. (2002). New application of 12-tungstophosphoric acid and its magnesium salt against plant viruses. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 52(4), 814-815.
https://hdl.handle.net/21.15107/rcub_agrospace_377

Uskoković-Marković SM, Todorović MR, Mioč UB, Krstić B, Dukić N. New application of 12-tungstophosphoric acid and its magnesium salt against plant viruses. in Arhiv za farmaciju. 2002;52(4):814-815.
https://hdl.handle.net/21.15107/rcub_agrospace_377 .

Uskoković-Marković, Snežana M., Todorović, Marija R., Mioč, Ubavka B., Krstić, Branka, Dukić, Nataša, "New application of 12-tungstophosphoric acid and its magnesium salt against plant viruses" in Arhiv za farmaciju, 52, no. 4 (2002):814-815,
https://hdl.handle.net/21.15107/rcub_agrospace_377 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Katis, Nikolaos I.
AU  - Papavassiliou, Chryssa
AU  - Berenji, Janoš
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/413
AB  - A survey on summer squash open field crops was carried out during 2000 and 2001 in order to identify the major viruses infecting these crops in different localities. Plants showed different types of symptoms: mild mosaic, chlorotic spotting, distinctive mosaic, blistering of leaf lamina leaf yellowing, deformation of leaf lamina, knobbed fruits and stunting of plants. The symptoms were very variable but showed the viral nature of the investigated summer squash diseases. The collected samples were tested by bioassay and by two serological methods ELISA and EBIA using cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2), zucchini yellow flack potyvirus (ZYFV) watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) and cucurbit aphid-borne yellows polerovirus (CABYV) polyclonal antisera. In all tested samples single or mixed infection with ZYMV, CMV and WMV-2 was detected. The most prevalent virus infecting summer squash was ZYMV. This is the first report of ZYMV, the most destructive virus infecting cucurbits, in Yugoslavia. It was also proven that the identified viruses are transmissible by Aphis gossypii in a non-persistent manner, but possible role of seed in virus transmission was not confirmed.
AB  - U toku 2000. i 2001. godine izvršen je pregled useva tikvica za jelo da bi se identifikovali osnovni virusi infektivni za tikvice u različitim lokalitetima. Biljke su pokazivale različite simptome: blagi mozaik hlorotičnu pegavost, izraženi mozaik, klobučavost liske, žućenje lista deformacije liske, bradavičaste izraštaje na plodu i kržljavost biljaka. Simptomi su veoma varijabilni i na osnovu njih se ne može obaviti determinacija virusa prouzrokovača oboljenja. Sakupljeni uzorci su testirani biotestom, kao i sa dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antiseruma na cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2) zucchini yellow flack potyvirus (ZYFV), watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) i cucurbit aphid-borne yellows polerovirus (CABYV). U svim ispitivanim uzorcima dokazana je pojedinačna ili mešana infekcija sa ZYMV, WMV-2 i CMV. Najčešće infekcije su bile sa ZYMV. Ovaj virus, jedan od najdestruktivnijih virusa na vrežastim kulturama, prvi put je konstatovan u našoj zemlji. Takođe je utvrđeno da se identifikovani virusi prenose na neperzistentan način vašima Aphis gossypii, a moguća uloga semena u pojavi oboljenja nije potvrđena.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Biological and serological characterization of viruses of summer squash crops in Yugoslavia
T1  - Biološka i serološka karakterizacija virusa tikvica u Jugoslaviji
EP  - 160
IS  - 2
SP  - 149
VL  - 47
DO  - 10.2298/JAS0202149D
ER  - 

@article{
author = "Dukić, Nataša and Krstić, Branka and Vico, Ivana and Katis, Nikolaos I. and Papavassiliou, Chryssa and Berenji, Janoš",
year = "2002",
abstract = "A survey on summer squash open field crops was carried out during 2000 and 2001 in order to identify the major viruses infecting these crops in different localities. Plants showed different types of symptoms: mild mosaic, chlorotic spotting, distinctive mosaic, blistering of leaf lamina leaf yellowing, deformation of leaf lamina, knobbed fruits and stunting of plants. The symptoms were very variable but showed the viral nature of the investigated summer squash diseases. The collected samples were tested by bioassay and by two serological methods ELISA and EBIA using cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2), zucchini yellow flack potyvirus (ZYFV) watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) and cucurbit aphid-borne yellows polerovirus (CABYV) polyclonal antisera. In all tested samples single or mixed infection with ZYMV, CMV and WMV-2 was detected. The most prevalent virus infecting summer squash was ZYMV. This is the first report of ZYMV, the most destructive virus infecting cucurbits, in Yugoslavia. It was also proven that the identified viruses are transmissible by Aphis gossypii in a non-persistent manner, but possible role of seed in virus transmission was not confirmed., U toku 2000. i 2001. godine izvršen je pregled useva tikvica za jelo da bi se identifikovali osnovni virusi infektivni za tikvice u različitim lokalitetima. Biljke su pokazivale različite simptome: blagi mozaik hlorotičnu pegavost, izraženi mozaik, klobučavost liske, žućenje lista deformacije liske, bradavičaste izraštaje na plodu i kržljavost biljaka. Simptomi su veoma varijabilni i na osnovu njih se ne može obaviti determinacija virusa prouzrokovača oboljenja. Sakupljeni uzorci su testirani biotestom, kao i sa dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antiseruma na cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2) zucchini yellow flack potyvirus (ZYFV), watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) i cucurbit aphid-borne yellows polerovirus (CABYV). U svim ispitivanim uzorcima dokazana je pojedinačna ili mešana infekcija sa ZYMV, WMV-2 i CMV. Najčešće infekcije su bile sa ZYMV. Ovaj virus, jedan od najdestruktivnijih virusa na vrežastim kulturama, prvi put je konstatovan u našoj zemlji. Takođe je utvrđeno da se identifikovani virusi prenose na neperzistentan način vašima Aphis gossypii, a moguća uloga semena u pojavi oboljenja nije potvrđena.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Biological and serological characterization of viruses of summer squash crops in Yugoslavia, Biološka i serološka karakterizacija virusa tikvica u Jugoslaviji",
pages = "160-149",
number = "2",
volume = "47",
doi = "10.2298/JAS0202149D"
}

Dukić, N., Krstić, B., Vico, I., Katis, N. I., Papavassiliou, C.,& Berenji, J.. (2002). Biological and serological characterization of viruses of summer squash crops in Yugoslavia. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 47(2), 149-160.
https://doi.org/10.2298/JAS0202149D

Dukić N, Krstić B, Vico I, Katis NI, Papavassiliou C, Berenji J. Biological and serological characterization of viruses of summer squash crops in Yugoslavia. in Journal of Agricultural Sciences (Belgrade). 2002;47(2):149-160.
doi:10.2298/JAS0202149D .

Dukić, Nataša, Krstić, Branka, Vico, Ivana, Katis, Nikolaos I., Papavassiliou, Chryssa, Berenji, Janoš, "Biological and serological characterization of viruses of summer squash crops in Yugoslavia" in Journal of Agricultural Sciences (Belgrade), 47, no. 2 (2002):149-160,
https://doi.org/10.2298/JAS0202149D . .

TY  - JOUR
AU  - Krstić, Branka
AU  - Berenji, Janoš
AU  - Dukić, Nataša
AU  - Vico, Ivana
AU  - Katis, Nikolaos I.
AU  - Papavassiliou, Chryssa
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/468
AB  - This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses infecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare.
AB  - Cilj ovih istraživanja bio je da se identifikuju najvažniji virusi tikava (Cucurbita pepo L) gajenih u Srbiji. Uzorci biljnog materijala uljane tikve-golice, uljane tikve sa ljuskom, tikvice za jelo, patisona i cukinija koji su bili zaraženi virusima, sakupljeni su u tri lokaliteta sredinom jula 2001. godine (tab. 1). Biljke zaražene virusima pokazivale su različite simptome (tab. 2 i sl. 1-4). Tačna determinacija virusa samo na osnovu simptoma nije moguća zbog varijabilnosti samih simptoma. Zaraženi uzorci su testirani biotestom kao i primenom dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antitela na Cucumber mosaic cucomovirus (CMV) Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus 2 (WMV-2), Watermelon mosaic potyvirus 1 (WMV-1) i Squash mosaic comovirus (SqMV). U 50 ispitanih uzoraka detektovan je jedan ili dva virusa (tab. 3 i 4). Preovlađujući virusi tikava bili su ZYMV (62%) i CMV (58%). WMV-2 je detektovan u veoma malom broju uzoraka.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia
T1  - Identifikacija virusa infektivnih za običnu tikvu (Cucurbita pepo L.) u Jugoslaviji
EP  - 79
IS  - 103
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_468
ER  - 

@article{
author = "Krstić, Branka and Berenji, Janoš and Dukić, Nataša and Vico, Ivana and Katis, Nikolaos I. and Papavassiliou, Chryssa",
year = "2002",
abstract = "This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses infecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare., Cilj ovih istraživanja bio je da se identifikuju najvažniji virusi tikava (Cucurbita pepo L) gajenih u Srbiji. Uzorci biljnog materijala uljane tikve-golice, uljane tikve sa ljuskom, tikvice za jelo, patisona i cukinija koji su bili zaraženi virusima, sakupljeni su u tri lokaliteta sredinom jula 2001. godine (tab. 1). Biljke zaražene virusima pokazivale su različite simptome (tab. 2 i sl. 1-4). Tačna determinacija virusa samo na osnovu simptoma nije moguća zbog varijabilnosti samih simptoma. Zaraženi uzorci su testirani biotestom kao i primenom dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antitela na Cucumber mosaic cucomovirus (CMV) Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus 2 (WMV-2), Watermelon mosaic potyvirus 1 (WMV-1) i Squash mosaic comovirus (SqMV). U 50 ispitanih uzoraka detektovan je jedan ili dva virusa (tab. 3 i 4). Preovlađujući virusi tikava bili su ZYMV (62%) i CMV (58%). WMV-2 je detektovan u veoma malom broju uzoraka.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia, Identifikacija virusa infektivnih za običnu tikvu (Cucurbita pepo L.) u Jugoslaviji",
pages = "79-67",
number = "103",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_468"
}

Krstić, B., Berenji, J., Dukić, N., Vico, I., Katis, N. I.,& Papavassiliou, C.. (2002). Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(103), 67-79.
https://hdl.handle.net/21.15107/rcub_agrospace_468

Krstić B, Berenji J, Dukić N, Vico I, Katis NI, Papavassiliou C. Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia. in Zbornik Matice srpske za prirodne nauke. 2002;(103):67-79.
https://hdl.handle.net/21.15107/rcub_agrospace_468 .

Krstić, Branka, Berenji, Janoš, Dukić, Nataša, Vico, Ivana, Katis, Nikolaos I., Papavassiliou, Chryssa, "Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia" in Zbornik Matice srpske za prirodne nauke, no. 103 (2002):67-79,
https://hdl.handle.net/21.15107/rcub_agrospace_468 .

TY  - JOUR
AU  - Vico, Ivana
AU  - Krstić, Branka
AU  - Dukić, Nataša
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/412
AB  - Antigenic properties and serological relationship was investigated in binucleate and multinucleate Rhizoctonia spp. isolates from strawberries soybean, alfalfa and potato plants from Serbia, from Spain, anastomosis group testers and in strawberry roots inoculated with binucleate Rhizoctonia AG A and AG I. Two polyclonal antisera, unabsorbed and cross absorbed, were used in dot-immunobinding assay for these investigations. Antisera were produced against mycelial antigens of two isolates, which belong to different anastomosis groups (AG) of binucleate Rhizoctonia - AG A and AG I. Both unabsorbed antisera reacted positively with all tested Rhizoctonia spp. isolates, and the reaction was absent with control isolates (Pythium sp. Agaricus sp. and Fusarium sp). The results prove a close serological relationship among Rhizoctonia spp. isolates, and diversity between Rhizoctonia spp. and isolates from different taxonomic groups. Also, both unabsorbed antisera reacted with higher intensity with closely related antigens (belonging to the same AG) than with ones from another AG of binucleate Rhizoctonia or R. solani (multinucleate Rhizoctonia). After cross absorption specificity of the antisera was enhanced, especially with the antiserum raised against mycelial proteins of binucleate Rhizoctonia AG I. This antiserum reacted positively only with antigens from the same AG, after cross absorption with antigens from AG A of binucleate Rhizoctonia and from R. solani AG 2-2. It proved to be specific to AG I of binucleate Rhizoctonia, and able to differentiate isolates of this AG from others. In this way the serological homology among isolates of one AG was proven, and also the diversity among isolates which belong to different AGs of binucleate Rhizoctonia as well as isolates of R. solani.
AB  - Antigene osobine i serološki medjuodnosi ispitivani su kod dvojedarnih i višejedarnih Rhizoctonia spp., izolovanih iz jagode, soje, lucerke i krompira prikupljenih na teritoriji Srbije, izolata iz Španije, standard izolata anastomoznih grupa i izolata prisutnih u zaraženom korenu jagode. Ispitivanja su obavljena dot-blot metodom primenom dva poliklonalna antiseruma, pre i posle unakrsne apsorpcije. Antiserumi su proizvedeni na miceliju dva izolata, pripadnika različitih anastomoznih grupa (AG) dvojedarnih Rhizoctonia spp. - AG A i AG I. Neapsorbovani antiserumi pozitivno su reagovali sa svim ispitivanim izolatima Rhizoctonia spp.,dok su reakcije izostale sa kontrolnim izolatima (Pythium sp., Agaricus sp. i Fusarium sp), što dokazuje serološku srodnost izmedju izolata Rhizoctonia spp., kao i serološku različitost Rhizoctonia spp. od izolata drugih taksonomskih grupa. Osim toga, reakcija oba neapsorbovana antiseruma bila je intenzivnija sa srodnim izolatima (pripadnicima iste AG), nego sa izolatima druge AG dvojedarnih Rhizoctonia ili R. solani (višejedarna Rhizoctonia) što govori o većoj serološkoj srodnosti u okviru jedne AG. Unakrsnom apsorpcijom antiseruma srodnost izolata iz iste grupe je i potvrdjena. Specifičnost antiseruma na ovaj način se povećava, što je naročito izraženo kod antiseruma proizvedenog na proteine micelije dvojedarne Rhizoctonia AG I. Ovaj antiserum je, nakon apsorpcije antigenom iz AG A i R. solani AG-2-2 pozitivno reagovao samo sa homologim antigenima (izolati iz AG I), odnosno ovaj antiserum može razlikovati izolate AG I dvojedarnih Rhizoctonia od ostalih Rhizoctonia spp. Na ovaj način potvrdjena je serološka srodnost izmedju izolata iste AG, i serološke razlike u odnosu na pripadnike drugih AG kako dvojedarnih Rhizoctonia spp. tako i R. solani.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Differentiation of Rhizoctonia spp. Based on their antigenic properties
T1  - Razlikovanje Rhizoctonia spp. Na osnovu antigenih osobina
EP  - 147
IS  - 2
SP  - 137
VL  - 47
DO  - 10.2298/JAS0202137V
ER  - 

@article{
author = "Vico, Ivana and Krstić, Branka and Dukić, Nataša",
year = "2002",
abstract = "Antigenic properties and serological relationship was investigated in binucleate and multinucleate Rhizoctonia spp. isolates from strawberries soybean, alfalfa and potato plants from Serbia, from Spain, anastomosis group testers and in strawberry roots inoculated with binucleate Rhizoctonia AG A and AG I. Two polyclonal antisera, unabsorbed and cross absorbed, were used in dot-immunobinding assay for these investigations. Antisera were produced against mycelial antigens of two isolates, which belong to different anastomosis groups (AG) of binucleate Rhizoctonia - AG A and AG I. Both unabsorbed antisera reacted positively with all tested Rhizoctonia spp. isolates, and the reaction was absent with control isolates (Pythium sp. Agaricus sp. and Fusarium sp). The results prove a close serological relationship among Rhizoctonia spp. isolates, and diversity between Rhizoctonia spp. and isolates from different taxonomic groups. Also, both unabsorbed antisera reacted with higher intensity with closely related antigens (belonging to the same AG) than with ones from another AG of binucleate Rhizoctonia or R. solani (multinucleate Rhizoctonia). After cross absorption specificity of the antisera was enhanced, especially with the antiserum raised against mycelial proteins of binucleate Rhizoctonia AG I. This antiserum reacted positively only with antigens from the same AG, after cross absorption with antigens from AG A of binucleate Rhizoctonia and from R. solani AG 2-2. It proved to be specific to AG I of binucleate Rhizoctonia, and able to differentiate isolates of this AG from others. In this way the serological homology among isolates of one AG was proven, and also the diversity among isolates which belong to different AGs of binucleate Rhizoctonia as well as isolates of R. solani., Antigene osobine i serološki medjuodnosi ispitivani su kod dvojedarnih i višejedarnih Rhizoctonia spp., izolovanih iz jagode, soje, lucerke i krompira prikupljenih na teritoriji Srbije, izolata iz Španije, standard izolata anastomoznih grupa i izolata prisutnih u zaraženom korenu jagode. Ispitivanja su obavljena dot-blot metodom primenom dva poliklonalna antiseruma, pre i posle unakrsne apsorpcije. Antiserumi su proizvedeni na miceliju dva izolata, pripadnika različitih anastomoznih grupa (AG) dvojedarnih Rhizoctonia spp. - AG A i AG I. Neapsorbovani antiserumi pozitivno su reagovali sa svim ispitivanim izolatima Rhizoctonia spp.,dok su reakcije izostale sa kontrolnim izolatima (Pythium sp., Agaricus sp. i Fusarium sp), što dokazuje serološku srodnost izmedju izolata Rhizoctonia spp., kao i serološku različitost Rhizoctonia spp. od izolata drugih taksonomskih grupa. Osim toga, reakcija oba neapsorbovana antiseruma bila je intenzivnija sa srodnim izolatima (pripadnicima iste AG), nego sa izolatima druge AG dvojedarnih Rhizoctonia ili R. solani (višejedarna Rhizoctonia) što govori o većoj serološkoj srodnosti u okviru jedne AG. Unakrsnom apsorpcijom antiseruma srodnost izolata iz iste grupe je i potvrdjena. Specifičnost antiseruma na ovaj način se povećava, što je naročito izraženo kod antiseruma proizvedenog na proteine micelije dvojedarne Rhizoctonia AG I. Ovaj antiserum je, nakon apsorpcije antigenom iz AG A i R. solani AG-2-2 pozitivno reagovao samo sa homologim antigenima (izolati iz AG I), odnosno ovaj antiserum može razlikovati izolate AG I dvojedarnih Rhizoctonia od ostalih Rhizoctonia spp. Na ovaj način potvrdjena je serološka srodnost izmedju izolata iste AG, i serološke razlike u odnosu na pripadnike drugih AG kako dvojedarnih Rhizoctonia spp. tako i R. solani.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Differentiation of Rhizoctonia spp. Based on their antigenic properties, Razlikovanje Rhizoctonia spp. Na osnovu antigenih osobina",
pages = "147-137",
number = "2",
volume = "47",
doi = "10.2298/JAS0202137V"
}

Vico, I., Krstić, B.,& Dukić, N.. (2002). Differentiation of Rhizoctonia spp. Based on their antigenic properties. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 47(2), 137-147.
https://doi.org/10.2298/JAS0202137V

Vico I, Krstić B, Dukić N. Differentiation of Rhizoctonia spp. Based on their antigenic properties. in Journal of Agricultural Sciences (Belgrade). 2002;47(2):137-147.
doi:10.2298/JAS0202137V .

Vico, Ivana, Krstić, Branka, Dukić, Nataša, "Differentiation of Rhizoctonia spp. Based on their antigenic properties" in Journal of Agricultural Sciences (Belgrade), 47, no. 2 (2002):137-147,
https://doi.org/10.2298/JAS0202137V . .

TY  - JOUR
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Torbica, Miroslav
AU  - Dukić, Nataša
AU  - Antonijević, Dragutin
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/387
AB  - Seed infection plays an important role in TMV epidemiology in pepper crops Pepper seed infection with that virus can be external and internal. The internal infection is the reason why some studies of the possible disinfection of pepper seed, infected with TMV, showed different degree of efficiency. The investigations were done with seed of two pepper cultivars Kurtovska kapija (infection 49%, germination 94%), and Somborka (infection 66%, germination 95%). In order to obtain more reliable methods of disinfection, infected pepper seed was soaked into 5,10,15 and 20% concentration of trisodium phosphate solution during 1 to 14 days at 25°C and 10, 20 and 30% concentration of sodium hypohlorite solution for two hours at 25, 35 and 45°C. Sodium hypohlorite treatments were not effective in virus elimination. Besides, they reduced germination of treated seed. Trisodium phosphate has already significantly decreased seed infection at a low concentration of 5% after a few days of treatment, while the virus has been completely eliminated at higher concentrations (20%) after ten days with seed of Kurtovska kapija and 12 days with seed of Somborka. Applied treatments of trisodium phosphate did not affect the germination of treated seed, so for disinfection of pepper seed. Therefore, trisodium phosphate in concentration of 15-20% is recommended for pepper seed disinfection. That disinfection should be done at the temperature of 25°C.
AB  - Za dezinfekciju semena paprike zaraženog virusom mozaika duvana u visokom procentu, primenjeni su trinatrijum-fosfat i natrijum-hipohlorit. Primenom natrijum-hipohlorita (30% rastvor, dva časa na 45" C) značajno je smanjena kako zaraženost, tako i klijavost tretiranog semena. Rastvor trinatrijum-fosfata koncentracije 20%, posle 10 dana u potpunosti dezinfikuje zaraženo seme sorte kurtovska kapija, dok je za dezinfekciju semena sorte somborka potrebno potapanje u 15% rastvor u trajanju od 12 dana.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Disinfection of pepper seed infected with tobacco mosaic virus
T1  - Dezinfekcija semena paprike zaraženog virusom mozaika duvana
EP  - 37
IS  - 1
SP  - 27
VL  - 53
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_387
ER  - 

@article{
author = "Krstić, Branka and Vico, Ivana and Torbica, Miroslav and Dukić, Nataša and Antonijević, Dragutin",
year = "2002",
abstract = "Seed infection plays an important role in TMV epidemiology in pepper crops Pepper seed infection with that virus can be external and internal. The internal infection is the reason why some studies of the possible disinfection of pepper seed, infected with TMV, showed different degree of efficiency. The investigations were done with seed of two pepper cultivars Kurtovska kapija (infection 49%, germination 94%), and Somborka (infection 66%, germination 95%). In order to obtain more reliable methods of disinfection, infected pepper seed was soaked into 5,10,15 and 20% concentration of trisodium phosphate solution during 1 to 14 days at 25°C and 10, 20 and 30% concentration of sodium hypohlorite solution for two hours at 25, 35 and 45°C. Sodium hypohlorite treatments were not effective in virus elimination. Besides, they reduced germination of treated seed. Trisodium phosphate has already significantly decreased seed infection at a low concentration of 5% after a few days of treatment, while the virus has been completely eliminated at higher concentrations (20%) after ten days with seed of Kurtovska kapija and 12 days with seed of Somborka. Applied treatments of trisodium phosphate did not affect the germination of treated seed, so for disinfection of pepper seed. Therefore, trisodium phosphate in concentration of 15-20% is recommended for pepper seed disinfection. That disinfection should be done at the temperature of 25°C., Za dezinfekciju semena paprike zaraženog virusom mozaika duvana u visokom procentu, primenjeni su trinatrijum-fosfat i natrijum-hipohlorit. Primenom natrijum-hipohlorita (30% rastvor, dva časa na 45" C) značajno je smanjena kako zaraženost, tako i klijavost tretiranog semena. Rastvor trinatrijum-fosfata koncentracije 20%, posle 10 dana u potpunosti dezinfikuje zaraženo seme sorte kurtovska kapija, dok je za dezinfekciju semena sorte somborka potrebno potapanje u 15% rastvor u trajanju od 12 dana.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Disinfection of pepper seed infected with tobacco mosaic virus, Dezinfekcija semena paprike zaraženog virusom mozaika duvana",
pages = "37-27",
number = "1",
volume = "53",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_387"
}

Krstić, B., Vico, I., Torbica, M., Dukić, N.,& Antonijević, D.. (2002). Disinfection of pepper seed infected with tobacco mosaic virus. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 53(1), 27-37.
https://hdl.handle.net/21.15107/rcub_agrospace_387

Krstić B, Vico I, Torbica M, Dukić N, Antonijević D. Disinfection of pepper seed infected with tobacco mosaic virus. in Zaštita bilja. 2002;53(1):27-37.
https://hdl.handle.net/21.15107/rcub_agrospace_387 .

Krstić, Branka, Vico, Ivana, Torbica, Miroslav, Dukić, Nataša, Antonijević, Dragutin, "Disinfection of pepper seed infected with tobacco mosaic virus" in Zaštita bilja, 53, no. 1 (2002):27-37,
https://hdl.handle.net/21.15107/rcub_agrospace_387 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Dukić, Nataša
AU  - Vučelić-Radović, Biljana
AU  - Barać, Miroljub
PY  - 2001
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/312
AB  - Infection of maize plants with two different viruses: maize dwarf mosaic potyvirus (MDMV) or sugarcane mosaic potyvirus-strain Yu (SCMV-YU), which produced the same type of symptom, resulted in identical changes in peroxidase activity and changes in the profiles of peroxidase isoenzymes. The peroxidase activity kept changing during infection. An increase in the activity of peroxidase was evident at the early stages of the infection. Very low activity of this enzyme was recorded on the 9th day after inoculation when clear symptoms of virus infection were expressed. The level of peroxidase activity was also dependent on the age of the infected leaves. The change in peroxidase activity was negligible in mature leaves and very pronounced in young fast growing leaves.The isoenzyme profiles of peroxidase investigated in this study, suggested that infection with MDMV or SCMV-YU involved in the senescence reaction was produced by the virus infection.
AB  - Infekcija biljaka kukuruza virusima mozaične kržljavosti kukuruza ili mozaika šećerne trske-soj Yu, koji prouzrokuju isti tip simptoma, izaziva identične promené u aktivnosti i profilima izoenzima peroksidaze. Aktivnost peroksidaze se menja u toku infekcije sa povećanjem na početku infekcije. Veoma niska aktivnost ovog enzima zabeležena je devetog dana po inokulaciji u vreme kada su ispoljeni jasni simptomi virusne infekcije. Nivo aktivnosti peroksidaze zavisi i od starosti lišća. Promene aktivnosti peroksidaze u listovima završenog rasta su beznačajne, a veoma izražene u listovima intenzivnog rasta. Utvrđeno je postojanje 19 izoenzima različite molekulske mase, čija jačina ispoljavanja u različitim danima po inokulaciji upućuje na to da infekcije ispitivanim virusima izazivaju reakcije karakteristične za starenje biljke.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Peroxidase activity and isoenzymes profiles in maize dwarf mosaic potyvirus leaves
T1  - Aktivnost i profili izoenzima peroksidaze u virozno mozaičnom lišću kukuruza
EP  - 64
IS  - 1
SP  - 51
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_312
ER  - 

@article{
author = "Krstić, Branka and Dukić, Nataša and Vučelić-Radović, Biljana and Barać, Miroljub",
year = "2001",
abstract = "Infection of maize plants with two different viruses: maize dwarf mosaic potyvirus (MDMV) or sugarcane mosaic potyvirus-strain Yu (SCMV-YU), which produced the same type of symptom, resulted in identical changes in peroxidase activity and changes in the profiles of peroxidase isoenzymes. The peroxidase activity kept changing during infection. An increase in the activity of peroxidase was evident at the early stages of the infection. Very low activity of this enzyme was recorded on the 9th day after inoculation when clear symptoms of virus infection were expressed. The level of peroxidase activity was also dependent on the age of the infected leaves. The change in peroxidase activity was negligible in mature leaves and very pronounced in young fast growing leaves.The isoenzyme profiles of peroxidase investigated in this study, suggested that infection with MDMV or SCMV-YU involved in the senescence reaction was produced by the virus infection., Infekcija biljaka kukuruza virusima mozaične kržljavosti kukuruza ili mozaika šećerne trske-soj Yu, koji prouzrokuju isti tip simptoma, izaziva identične promené u aktivnosti i profilima izoenzima peroksidaze. Aktivnost peroksidaze se menja u toku infekcije sa povećanjem na početku infekcije. Veoma niska aktivnost ovog enzima zabeležena je devetog dana po inokulaciji u vreme kada su ispoljeni jasni simptomi virusne infekcije. Nivo aktivnosti peroksidaze zavisi i od starosti lišća. Promene aktivnosti peroksidaze u listovima završenog rasta su beznačajne, a veoma izražene u listovima intenzivnog rasta. Utvrđeno je postojanje 19 izoenzima različite molekulske mase, čija jačina ispoljavanja u različitim danima po inokulaciji upućuje na to da infekcije ispitivanim virusima izazivaju reakcije karakteristične za starenje biljke.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Peroxidase activity and isoenzymes profiles in maize dwarf mosaic potyvirus leaves, Aktivnost i profili izoenzima peroksidaze u virozno mozaičnom lišću kukuruza",
pages = "64-51",
number = "1",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_312"
}

Krstić, B., Dukić, N., Vučelić-Radović, B.,& Barać, M.. (2001). Peroxidase activity and isoenzymes profiles in maize dwarf mosaic potyvirus leaves. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 52(1), 51-64.
https://hdl.handle.net/21.15107/rcub_agrospace_312

Krstić B, Dukić N, Vučelić-Radović B, Barać M. Peroxidase activity and isoenzymes profiles in maize dwarf mosaic potyvirus leaves. in Zaštita bilja. 2001;52(1):51-64.
https://hdl.handle.net/21.15107/rcub_agrospace_312 .

Krstić, Branka, Dukić, Nataša, Vučelić-Radović, Biljana, Barać, Miroljub, "Peroxidase activity and isoenzymes profiles in maize dwarf mosaic potyvirus leaves" in Zaštita bilja, 52, no. 1 (2001):51-64,
https://hdl.handle.net/21.15107/rcub_agrospace_312 .