TY  - JOUR
AU  - Stojanović, Branislav
AU  - Janković, Saša
AU  - Đorđević, Vesna Ž.
AU  - Marjanović, Sonja
AU  - Vasilev, Dragan
AU  - Stojanović, Zdenka
AU  - Balaban, Milica
AU  - Antić, Vesna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5854
AB  - This work aimed to examine the influence of the storage period on the content of toxic elements (As, Cd, Hg, and Pb) in five types of canned meat products regularly used in the Serbian Armed Forces. Cans of beef goulash (BG), pork ragout (PR), spam (SP), liver pate (LP), and meatballs in tomato sauce (MB), produced according to military standards and stored under regular conditions, were analyzed. Meat products were packed in tin cans made according to special requirements in terms of tin and varnish application and stored for up to 6 years. The content of toxic elements varied depending on the analyzed product. The highest average content of arsenic was in BG (10.00 μg/kg), cadmium in LP (35.91 μg/kg), and mercury and lead in PR (15.04 and 8.00 μg/kg, respectively). The average concentrations of As, Cd, Hg, and Pb in all types of canned meat products were significantly lower than the maximum permitted levels in food currently in force by local and EU legislation. The storage period did not significantly affect the level of toxic elements, although higher concentrations were found in samples stored for more than 2 years. Examination of raw materials, spices, and additives showed that the highest Cd and Pb concentrations, which can affect the total level of these elements in meat products, were found in red ground pepper (Cd above 150 μg/kg) and dish supplement (Pb of 250 μg/kg). The assessment of the weekly intake of toxic elements through canned meat showed that it is significantly lower than the values that affect adversely to human health, as determined by the FAO/WHO and EFSA. However, as there is a constant possibility of contamination of raw materials and food additives, primarily due to environmental pollution, it is recommended to monitor the content of heavy metals in food permanently and assess their risk to human health.
PB  - Springer Link
T2  - Environmental Science and Pollution Research
T1  - Determination of toxic elements in meat products from Serbia packaged in tinplate cans
DO  - 10.1007/s11356-021-14051-w
ER  - 

@article{
author = "Stojanović, Branislav and Janković, Saša and Đorđević, Vesna Ž. and Marjanović, Sonja and Vasilev, Dragan and Stojanović, Zdenka and Balaban, Milica and Antić, Vesna",
year = "2021",
abstract = "This work aimed to examine the influence of the storage period on the content of toxic elements (As, Cd, Hg, and Pb) in five types of canned meat products regularly used in the Serbian Armed Forces. Cans of beef goulash (BG), pork ragout (PR), spam (SP), liver pate (LP), and meatballs in tomato sauce (MB), produced according to military standards and stored under regular conditions, were analyzed. Meat products were packed in tin cans made according to special requirements in terms of tin and varnish application and stored for up to 6 years. The content of toxic elements varied depending on the analyzed product. The highest average content of arsenic was in BG (10.00 μg/kg), cadmium in LP (35.91 μg/kg), and mercury and lead in PR (15.04 and 8.00 μg/kg, respectively). The average concentrations of As, Cd, Hg, and Pb in all types of canned meat products were significantly lower than the maximum permitted levels in food currently in force by local and EU legislation. The storage period did not significantly affect the level of toxic elements, although higher concentrations were found in samples stored for more than 2 years. Examination of raw materials, spices, and additives showed that the highest Cd and Pb concentrations, which can affect the total level of these elements in meat products, were found in red ground pepper (Cd above 150 μg/kg) and dish supplement (Pb of 250 μg/kg). The assessment of the weekly intake of toxic elements through canned meat showed that it is significantly lower than the values that affect adversely to human health, as determined by the FAO/WHO and EFSA. However, as there is a constant possibility of contamination of raw materials and food additives, primarily due to environmental pollution, it is recommended to monitor the content of heavy metals in food permanently and assess their risk to human health.",
publisher = "Springer Link",
journal = "Environmental Science and Pollution Research",
title = "Determination of toxic elements in meat products from Serbia packaged in tinplate cans",
doi = "10.1007/s11356-021-14051-w"
}

Stojanović, B., Janković, S., Đorđević, V. Ž., Marjanović, S., Vasilev, D., Stojanović, Z., Balaban, M.,& Antić, V.. (2021). Determination of toxic elements in meat products from Serbia packaged in tinplate cans. in Environmental Science and Pollution Research
Springer Link..
https://doi.org/10.1007/s11356-021-14051-w

Stojanović B, Janković S, Đorđević VŽ, Marjanović S, Vasilev D, Stojanović Z, Balaban M, Antić V. Determination of toxic elements in meat products from Serbia packaged in tinplate cans. in Environmental Science and Pollution Research. 2021;.
doi:10.1007/s11356-021-14051-w .

Stojanović, Branislav, Janković, Saša, Đorđević, Vesna Ž., Marjanović, Sonja, Vasilev, Dragan, Stojanović, Zdenka, Balaban, Milica, Antić, Vesna, "Determination of toxic elements in meat products from Serbia packaged in tinplate cans" in Environmental Science and Pollution Research (2021),
https://doi.org/10.1007/s11356-021-14051-w . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Stefanović, Srdjan
AU  - Janković, Saša
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1875
AB  - The aim of this paper was to provide detailed insight into the level of contamination of complete feeding mixtures intended for fattening pigs with mycotoxins-producing fungi and mycotoxins (n = 18). Isolation and enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated, the number of colonies was determined and moulds genera and species identification was carried out using colonies morphological determination and microscopic analysis. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide syntheses gene-PKS). Total fungal counts (CFU/g) ranged from 0,5x105 to 4x106. From a total samples analyzed, seven samples had fungal counts higher than the limit established by Serbian regulations (3x105). During a mycological analysis of complete feeding mixtures intended for fattening pigs, a total of six genera and 14 species of moulds were identified and the most frequent one was genus Penicillium (94,4%). The moulds from genus Fusarium were isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a lesser amount. Polymerase chain reaction (PCR) was performed on 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase gene (PKS) from Penicillium dance and found that the samples possessed PKS sequence. The traditional methods for identification of ochratoxin-producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochratoxin-producing fungi is important for ensuring microbiological quality and safety of feed and food. .
AB  - Radi procene rizika od mikotoksina obavljena su mikološka i mikotoksikološka ispitivanja uzoraka potpunih smeša za ishranu svinja koje potiču iz različitih regiona Srbije (n = 18). Ukupan broj plesni i njihova izolacija u uzorcima potpunih smeša za ishranu svinja, određivan je standardnom mikrobiološkom metodom. Nakon inkubiranja, na osnovu izgleda kolonija i mikroskopske analize obavljena je identifikacija rodova i vrsta plesni. Izolati koji su identifi kovani kao Aspergillus i Penicillium vrste podvrgnuti su molekularnoj karakterizaciji na prisustvo gena odgovornog za sintezu OTA (poliketid sinte taza). Ukupan broj plesni u ispitanim uzorcima bio je od 0,5 × 105 do 4 ×106 (CFU/g). U pogledu ukupnog broja plesni (CFU/g) sedam (38,3 posto) uzoraka bilo je kontaminirano plesnima iznad maksimalno dozvoljene količine (3 × 105). Analizom mikopopulacije uzoraka potpunih smeša za ishranu svinja izolovano je šest rodova i 14 vrsta plesni. Najčešće su izolovane plesni iz roda Penicillium (94,4 posto), zatim Fusarium (55,5 posto) i Paecilomyces (44,4 posto), dok su plesni iz roda Aspergillus (22 posto), Mucor (11,1 posto) i Alternaria (5,5 posto) izolovane u manjem procentu. Lančana reakcija polimeraze (PCR) postavljena je za 18 izolata DNK koje pripadaju rodovima Penicillium i Aspergillus. Sekvence proizvoda PCR reakcije kod tri uzorka poređene su sa nukleotidnim sekvencama gena za poliketid sintetazu (PKS) iz plesni Penicillium i ustanovljeno je da uzorci poseduju PKS sekvencu. Razvoj brzih metoda za determinaciju ohratoksogenih plesni ima za cilj unapređenje sistema kontrole bezbednosti i kvaliteta hrane. U svakoj fazi proizvodnje i prometa hrane može da se spreči, smanji ili eliminiše rizik po krajnjeg korisnika.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment
T1  - Prisustvo plesni i mikotoksina u hrani za ishranu svinja - značaj u proceni rizika
EP  - 270
IS  - 5-6
SP  - 261
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1875
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Stefanović, Srdjan and Janković, Saša",
year = "2009",
abstract = "The aim of this paper was to provide detailed insight into the level of contamination of complete feeding mixtures intended for fattening pigs with mycotoxins-producing fungi and mycotoxins (n = 18). Isolation and enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated, the number of colonies was determined and moulds genera and species identification was carried out using colonies morphological determination and microscopic analysis. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide syntheses gene-PKS). Total fungal counts (CFU/g) ranged from 0,5x105 to 4x106. From a total samples analyzed, seven samples had fungal counts higher than the limit established by Serbian regulations (3x105). During a mycological analysis of complete feeding mixtures intended for fattening pigs, a total of six genera and 14 species of moulds were identified and the most frequent one was genus Penicillium (94,4%). The moulds from genus Fusarium were isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a lesser amount. Polymerase chain reaction (PCR) was performed on 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase gene (PKS) from Penicillium dance and found that the samples possessed PKS sequence. The traditional methods for identification of ochratoxin-producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochratoxin-producing fungi is important for ensuring microbiological quality and safety of feed and food. ., Radi procene rizika od mikotoksina obavljena su mikološka i mikotoksikološka ispitivanja uzoraka potpunih smeša za ishranu svinja koje potiču iz različitih regiona Srbije (n = 18). Ukupan broj plesni i njihova izolacija u uzorcima potpunih smeša za ishranu svinja, određivan je standardnom mikrobiološkom metodom. Nakon inkubiranja, na osnovu izgleda kolonija i mikroskopske analize obavljena je identifikacija rodova i vrsta plesni. Izolati koji su identifi kovani kao Aspergillus i Penicillium vrste podvrgnuti su molekularnoj karakterizaciji na prisustvo gena odgovornog za sintezu OTA (poliketid sinte taza). Ukupan broj plesni u ispitanim uzorcima bio je od 0,5 × 105 do 4 ×106 (CFU/g). U pogledu ukupnog broja plesni (CFU/g) sedam (38,3 posto) uzoraka bilo je kontaminirano plesnima iznad maksimalno dozvoljene količine (3 × 105). Analizom mikopopulacije uzoraka potpunih smeša za ishranu svinja izolovano je šest rodova i 14 vrsta plesni. Najčešće su izolovane plesni iz roda Penicillium (94,4 posto), zatim Fusarium (55,5 posto) i Paecilomyces (44,4 posto), dok su plesni iz roda Aspergillus (22 posto), Mucor (11,1 posto) i Alternaria (5,5 posto) izolovane u manjem procentu. Lančana reakcija polimeraze (PCR) postavljena je za 18 izolata DNK koje pripadaju rodovima Penicillium i Aspergillus. Sekvence proizvoda PCR reakcije kod tri uzorka poređene su sa nukleotidnim sekvencama gena za poliketid sintetazu (PKS) iz plesni Penicillium i ustanovljeno je da uzorci poseduju PKS sekvencu. Razvoj brzih metoda za determinaciju ohratoksogenih plesni ima za cilj unapređenje sistema kontrole bezbednosti i kvaliteta hrane. U svakoj fazi proizvodnje i prometa hrane može da se spreči, smanji ili eliminiše rizik po krajnjeg korisnika.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment, Prisustvo plesni i mikotoksina u hrani za ishranu svinja - značaj u proceni rizika",
pages = "270-261",
number = "5-6",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1875"
}

Milićević, D., Nikšić, M., Baltić, T., Stefanović, S.,& Janković, S.. (2009). Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 50(5-6), 261-270.
https://hdl.handle.net/21.15107/rcub_agrospace_1875

Milićević D, Nikšić M, Baltić T, Stefanović S, Janković S. Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment. in Tehnologija mesa. 2009;50(5-6):261-270.
https://hdl.handle.net/21.15107/rcub_agrospace_1875 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Stefanović, Srdjan, Janković, Saša, "Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment" in Tehnologija mesa, 50, no. 5-6 (2009):261-270,
https://hdl.handle.net/21.15107/rcub_agrospace_1875 .