TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Branković, Gordana
AU  - Ahsyee, Ramadan Salem
AU  - Momirović, Una
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4331
AB  - Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Molecular characterization of red clover genotypes utilizing microsatellite markers
EP  - 47
IS  - 1
SP  - 41
VL  - 77
DO  - 10.4067/S0718-58392017000100005
ER  - 

@article{
author = "Radinović, Irena and Vasiljević, Sanja and Branković, Gordana and Ahsyee, Ramadan Salem and Momirović, Una and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2017",
abstract = "Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Molecular characterization of red clover genotypes utilizing microsatellite markers",
pages = "47-41",
number = "1",
volume = "77",
doi = "10.4067/S0718-58392017000100005"
}

Radinović, I., Vasiljević, S., Branković, G., Ahsyee, R. S., Momirović, U., Perović, D.,& Šurlan-Momirović, G.. (2017). Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 77(1), 41-47.
https://doi.org/10.4067/S0718-58392017000100005

Radinović I, Vasiljević S, Branković G, Ahsyee RS, Momirović U, Perović D, Šurlan-Momirović G. Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research. 2017;77(1):41-47.
doi:10.4067/S0718-58392017000100005 .

Radinović, Irena, Vasiljević, Sanja, Branković, Gordana, Ahsyee, Ramadan Salem, Momirović, Una, Perović, Dragan, Šurlan-Momirović, Gordana, "Molecular characterization of red clover genotypes utilizing microsatellite markers" in Chilean Journal of Agricultural Research, 77, no. 1 (2017):41-47,
https://doi.org/10.4067/S0718-58392017000100005 . .

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Flath, Kerstin
AU  - Silvar, Cristina
AU  - Branković, Gordana
AU  - Kopahnke, Doris
AU  - Knežević, Desimir
AU  - Schliephake, Edgar
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4151
AB  - Unlocking resistance genes in genbank collections are of prime importance for securing sustainable crop production. In this regard, the Serbian GenBank barley collection, comprising 93 local landraces and 36 commercial cultivars and elite barley breeding lines, was screened for novel resistances to powdery mildew (Blumeria graminis f. sp. hordei) using a set of 28 isolates with a wide spectrum of virulences/avirulences. No line was resistant to all the isolates, but one and three accessions showed resistance to 27 and 26 isolates, respectively. Twenty landraces (21.51 %) and ten cultivars (27.78 %) exhibited resistance to 50 % of the isolates. Infection type 2 was most frequent among resistant accessions. Nine B. graminis isolates were sufficient for gene postulation in 73 barley lines. In total, thirty-five different resistance spectra were recorded and the following known resistance genes were postulated namely, Mlra, Mlh, Mla12, Mla7(Mlu), Mlg, MlLa, Mla6, Mla7, Mlt, Mla22, Mlat, Mla1, Mlk. The majority of resistance profiles was constituted by only one line. Unidentified genes alone or in combination were proposed for twenty landraces and six cultivars. This report demonstrated that the barley collection held at the Serbian GenBank could be exploited as a new source for powdery mildew resistance.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance
EP  - 287
IS  - 2
SP  - 275
VL  - 63
DO  - 10.1007/s10722-015-0246-2
ER  - 

@article{
author = "Šurlan-Momirović, Gordana and Flath, Kerstin and Silvar, Cristina and Branković, Gordana and Kopahnke, Doris and Knežević, Desimir and Schliephake, Edgar and Ordon, Frank and Perović, Dragan",
year = "2016",
abstract = "Unlocking resistance genes in genbank collections are of prime importance for securing sustainable crop production. In this regard, the Serbian GenBank barley collection, comprising 93 local landraces and 36 commercial cultivars and elite barley breeding lines, was screened for novel resistances to powdery mildew (Blumeria graminis f. sp. hordei) using a set of 28 isolates with a wide spectrum of virulences/avirulences. No line was resistant to all the isolates, but one and three accessions showed resistance to 27 and 26 isolates, respectively. Twenty landraces (21.51 %) and ten cultivars (27.78 %) exhibited resistance to 50 % of the isolates. Infection type 2 was most frequent among resistant accessions. Nine B. graminis isolates were sufficient for gene postulation in 73 barley lines. In total, thirty-five different resistance spectra were recorded and the following known resistance genes were postulated namely, Mlra, Mlh, Mla12, Mla7(Mlu), Mlg, MlLa, Mla6, Mla7, Mlt, Mla22, Mlat, Mla1, Mlk. The majority of resistance profiles was constituted by only one line. Unidentified genes alone or in combination were proposed for twenty landraces and six cultivars. This report demonstrated that the barley collection held at the Serbian GenBank could be exploited as a new source for powdery mildew resistance.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance",
pages = "287-275",
number = "2",
volume = "63",
doi = "10.1007/s10722-015-0246-2"
}

Šurlan-Momirović, G., Flath, K., Silvar, C., Branković, G., Kopahnke, D., Knežević, D., Schliephake, E., Ordon, F.,& Perović, D.. (2016). Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance. in Genetic Resources and Crop Evolution
Springer, Dordrecht., 63(2), 275-287.
https://doi.org/10.1007/s10722-015-0246-2

Šurlan-Momirović G, Flath K, Silvar C, Branković G, Kopahnke D, Knežević D, Schliephake E, Ordon F, Perović D. Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance. in Genetic Resources and Crop Evolution. 2016;63(2):275-287.
doi:10.1007/s10722-015-0246-2 .

Šurlan-Momirović, Gordana, Flath, Kerstin, Silvar, Cristina, Branković, Gordana, Kopahnke, Doris, Knežević, Desimir, Schliephake, Edgar, Ordon, Frank, Perović, Dragan, "Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance" in Genetic Resources and Crop Evolution, 63, no. 2 (2016):275-287,
https://doi.org/10.1007/s10722-015-0246-2 . .

TY  - JOUR
AU  - Wang, S.L.
AU  - Chen, D.
AU  - Guo, G.F.
AU  - Zhang, T.
AU  - Jiang, S.S.
AU  - Shen, X.X.
AU  - Perović, D.
AU  - Prodanović, Slaven
AU  - Yan, Y.M.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3038
AB  - In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species
EP  - 551
IS  - 4
SP  - 542
VL  - 40
DO  - 10.1556/CRC.40.2012.0006
ER  - 

@article{
author = "Wang, S.L. and Chen, D. and Guo, G.F. and Zhang, T. and Jiang, S.S. and Shen, X.X. and Perović, D. and Prodanović, Slaven and Yan, Y.M.",
year = "2012",
abstract = "In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species",
pages = "551-542",
number = "4",
volume = "40",
doi = "10.1556/CRC.40.2012.0006"
}

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, S.,& Yan, Y.M.. (2012). Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 40(4), 542-551.
https://doi.org/10.1556/CRC.40.2012.0006

Wang S, Chen D, Guo G, Zhang T, Jiang S, Shen X, Perović D, Prodanović S, Yan Y. Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications. 2012;40(4):542-551.
doi:10.1556/CRC.40.2012.0006 .

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, Slaven, Yan, Y.M., "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species" in Cereal Research Communications, 40, no. 4 (2012):542-551,
https://doi.org/10.1556/CRC.40.2012.0006 . .