TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Krämer, Ilona
AU  - Bratković, Kamenko
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Branković, Gordana
AU  - Ćalić, Irena
AU  - Kandić, Vesna
AU  - Pržulj, Novo
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3123
AB  - Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes.
AB  - Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting
T1  - Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima
EP  - 180
IS  - 1
SP  - 167
VL  - 45
DO  - 10.2298/GENSR1301167S
ER  - 

@article{
author = "Šurlan-Momirović, Gordana and Krämer, Ilona and Bratković, Kamenko and Zorić, Miroslav and Momirović, Una and Branković, Gordana and Ćalić, Irena and Kandić, Vesna and Pržulj, Novo and Ordon, Frank and Perović, Dragan",
year = "2013",
abstract = "Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes., Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting, Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima",
pages = "180-167",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301167S"
}

Šurlan-Momirović, G., Krämer, I., Bratković, K., Zorić, M., Momirović, U., Branković, G., Ćalić, I., Kandić, V., Pržulj, N., Ordon, F.,& Perović, D.. (2013). Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika
Društvo genetičara Srbije, Beograd., 45(1), 167-180.
https://doi.org/10.2298/GENSR1301167S

Šurlan-Momirović G, Krämer I, Bratković K, Zorić M, Momirović U, Branković G, Ćalić I, Kandić V, Pržulj N, Ordon F, Perović D. Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika. 2013;45(1):167-180.
doi:10.2298/GENSR1301167S .

Šurlan-Momirović, Gordana, Krämer, Ilona, Bratković, Kamenko, Zorić, Miroslav, Momirović, Una, Branković, Gordana, Ćalić, Irena, Kandić, Vesna, Pržulj, Novo, Ordon, Frank, Perović, Dragan, "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting" in Genetika, 45, no. 1 (2013):167-180,
https://doi.org/10.2298/GENSR1301167S . .

TY  - JOUR
AU  - Perović, Dragan
AU  - Zorić, Dragica
AU  - Milovanović, Milivoje
AU  - Prodanović, Slaven
AU  - Yan, Yueming
AU  - Janković, Snežana
AU  - Šurlan-Momirović, Gordana
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2077
AB  - The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analyzed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis.
AB  - Prisustvo dva majčinska seta hromozoma u triploidnom endospermu zrna ječma omogućuje razlikovanje hordeinskih traka u elektroforegramu koje potiču od majke i oca: majčinske komponente su jače ispoljene usled dejstva efekta doze gena. U F1 generaciji vidljive su razlike kod recipročnog ukrštanja, a u F2 generaciji je moguće razlikovati svih 16 teoretski očekivanih klasa za par polimorfnih lokusa. Ova potpuna klasifikacija redak je fenomen u genetičkim studijama te zbog toga omogućuje precizniju procenu rekombinacija. Dve grupe hordeinskih gena, Hor1 i Hor2 koji kodiraju C I B hordeine, su analizirane kod hibrida nastalih ukrštanjem dve ozime sorte ječma Partizan i HWV-247. Separacija hordeina je izvršena primenom kisele poliakrilamidne gel elektriforeze pri pH 3.2 (A-PAGE). Set najinformativnijih hordeinskih trajka za B i C hordeine je odabran na osnovu sledećih kriterijuma: (1) prisustvo ili odsustvo trake kod roditelja i (2) jačina signala koja omogućava ocenu efekta doze gena. Prosečna genetička distance između Hor1 i Hor2 lokusa bila je 11 cM. Genetičke distance kod roditeljskih komponenti nisu se statistički značajno razlikovale što sugeriše sličnu distribuciju rekombinacija u obe mejoze.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.)
T1  - Efekti doze gena kod hordeina u triploidnom endospermu ječma (Hordeum vulgare L.)
EP  - 287
IS  - 3
SP  - 271
VL  - 41
DO  - 10.2298/GENSR0903271P
ER  - 

@article{
author = "Perović, Dragan and Zorić, Dragica and Milovanović, Milivoje and Prodanović, Slaven and Yan, Yueming and Janković, Snežana and Šurlan-Momirović, Gordana",
year = "2009",
abstract = "The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analyzed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis., Prisustvo dva majčinska seta hromozoma u triploidnom endospermu zrna ječma omogućuje razlikovanje hordeinskih traka u elektroforegramu koje potiču od majke i oca: majčinske komponente su jače ispoljene usled dejstva efekta doze gena. U F1 generaciji vidljive su razlike kod recipročnog ukrštanja, a u F2 generaciji je moguće razlikovati svih 16 teoretski očekivanih klasa za par polimorfnih lokusa. Ova potpuna klasifikacija redak je fenomen u genetičkim studijama te zbog toga omogućuje precizniju procenu rekombinacija. Dve grupe hordeinskih gena, Hor1 i Hor2 koji kodiraju C I B hordeine, su analizirane kod hibrida nastalih ukrštanjem dve ozime sorte ječma Partizan i HWV-247. Separacija hordeina je izvršena primenom kisele poliakrilamidne gel elektriforeze pri pH 3.2 (A-PAGE). Set najinformativnijih hordeinskih trajka za B i C hordeine je odabran na osnovu sledećih kriterijuma: (1) prisustvo ili odsustvo trake kod roditelja i (2) jačina signala koja omogućava ocenu efekta doze gena. Prosečna genetička distance između Hor1 i Hor2 lokusa bila je 11 cM. Genetičke distance kod roditeljskih komponenti nisu se statistički značajno razlikovale što sugeriše sličnu distribuciju rekombinacija u obe mejoze.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.), Efekti doze gena kod hordeina u triploidnom endospermu ječma (Hordeum vulgare L.)",
pages = "287-271",
number = "3",
volume = "41",
doi = "10.2298/GENSR0903271P"
}

Perović, D., Zorić, D., Milovanović, M., Prodanović, S., Yan, Y., Janković, S.,& Šurlan-Momirović, G.. (2009). Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.). in Genetika
Društvo genetičara Srbije, Beograd., 41(3), 271-287.
https://doi.org/10.2298/GENSR0903271P

Perović D, Zorić D, Milovanović M, Prodanović S, Yan Y, Janković S, Šurlan-Momirović G. Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.). in Genetika. 2009;41(3):271-287.
doi:10.2298/GENSR0903271P .

Perović, Dragan, Zorić, Dragica, Milovanović, Milivoje, Prodanović, Slaven, Yan, Yueming, Janković, Snežana, Šurlan-Momirović, Gordana, "Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.)" in Genetika, 41, no. 3 (2009):271-287,
https://doi.org/10.2298/GENSR0903271P . .