TY  - JOUR
AU  - Dashtbin, Rana
AU  - Mahmoudi, Nemat
AU  - Besharati, Hossein
AU  - Lalevic, Blažo
PY  - 2023
UR  - https://doi.org/10.1007/s42770-023-01128-6
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6477
AB  - Hydrogen sulfide is a highly toxic gas that causes many economic losses in aquaculture ponds. The application of sulfur-oxidizing bacteria (SOB) to remove hydrogen sulfide is an eco-friendly approach. This study aimed to isolate and identify the most efficient SOBs from the sediment of warm-water fish farms. Enrichment and isolation were performed in three different culture media (Starkey, Postgate, and H-3) based on both mineral and organic carbon. Overall, 27 isolates (14 autotrophic and 13 heterotrophic isolates) were purified based on colony and cell morphology differences. Initial screening was performed based on pH decrease. For final screening, the isolates were assessed based on their efficacy in thiosulfate oxidation and the sulfate production on Starkey liquid medium. Among isolated strains, 3 strains of Iran 2 (FH-13), Iran 3 (FH-21), and Iran 1 (FH-14) that belonged to Thiobacillus thioparus species (identified by 16s rRNA) showed the highest ability in thiosulfate oxidation (413.21, 1362.50, and 4188.03 mg/L for 14 days) and the highest sulfate production (3350, 2075, and 1600 mg/L). In the final phase, the performance of these strains under aquarium conditions showed that Iran 1 and Iran 2 had the highest ability in sulfur oxidation. In conclusion, Iran 1 and 2 strains can be used as effective SOB to remove hydrogen sulfide in fish farms. It is very important to evaluate strains in an appropriate strategy using a combination of different criteria to ensure optimal performance of SOB in farm conditions.
T2  - Brazilian Journal of Microbiology
T2  - Brazilian Journal of MicrobiologyBraz J Microbiol
T1  - Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions
DO  - 10.1007/s42770-023-01128-6
ER  - 

@article{
author = "Dashtbin, Rana and Mahmoudi, Nemat and Besharati, Hossein and Lalevic, Blažo",
year = "2023",
abstract = "Hydrogen sulfide is a highly toxic gas that causes many economic losses in aquaculture ponds. The application of sulfur-oxidizing bacteria (SOB) to remove hydrogen sulfide is an eco-friendly approach. This study aimed to isolate and identify the most efficient SOBs from the sediment of warm-water fish farms. Enrichment and isolation were performed in three different culture media (Starkey, Postgate, and H-3) based on both mineral and organic carbon. Overall, 27 isolates (14 autotrophic and 13 heterotrophic isolates) were purified based on colony and cell morphology differences. Initial screening was performed based on pH decrease. For final screening, the isolates were assessed based on their efficacy in thiosulfate oxidation and the sulfate production on Starkey liquid medium. Among isolated strains, 3 strains of Iran 2 (FH-13), Iran 3 (FH-21), and Iran 1 (FH-14) that belonged to Thiobacillus thioparus species (identified by 16s rRNA) showed the highest ability in thiosulfate oxidation (413.21, 1362.50, and 4188.03 mg/L for 14 days) and the highest sulfate production (3350, 2075, and 1600 mg/L). In the final phase, the performance of these strains under aquarium conditions showed that Iran 1 and Iran 2 had the highest ability in sulfur oxidation. In conclusion, Iran 1 and 2 strains can be used as effective SOB to remove hydrogen sulfide in fish farms. It is very important to evaluate strains in an appropriate strategy using a combination of different criteria to ensure optimal performance of SOB in farm conditions.",
journal = "Brazilian Journal of Microbiology, Brazilian Journal of MicrobiologyBraz J Microbiol",
title = "Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions",
doi = "10.1007/s42770-023-01128-6"
}

Dashtbin, R., Mahmoudi, N., Besharati, H.,& Lalevic, B.. (2023). Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions. in Brazilian Journal of Microbiology.
https://doi.org/10.1007/s42770-023-01128-6

Dashtbin R, Mahmoudi N, Besharati H, Lalevic B. Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions. in Brazilian Journal of Microbiology. 2023;.
doi:10.1007/s42770-023-01128-6 .

Dashtbin, Rana, Mahmoudi, Nemat, Besharati, Hossein, Lalevic, Blažo, "Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions" in Brazilian Journal of Microbiology (2023),
https://doi.org/10.1007/s42770-023-01128-6 . .