TY  - JOUR
AU  - Mićanović, Danica
AU  - Hirsch, Penny
AU  - Curnow, Phil
AU  - Zečević, Veselinka
AU  - Raičević, Vera
AU  - Knežević, Desimir
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1109
AB  - The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic resistance to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heterotroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Wheat colonization by determinate bacteria
EP  - 317
IS  - 3-4
SP  - 312
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1109
ER  - 

@article{
author = "Mićanović, Danica and Hirsch, Penny and Curnow, Phil and Zečević, Veselinka and Raičević, Vera and Knežević, Desimir",
year = "2005",
abstract = "The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic resistance to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heterotroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Wheat colonization by determinate bacteria",
pages = "317-312",
number = "3-4",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1109"
}

Mićanović, D., Hirsch, P., Curnow, P., Zečević, V., Raičević, V.,& Knežević, D.. (2005). Wheat colonization by determinate bacteria. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 54(3-4), 312-317.
https://hdl.handle.net/21.15107/rcub_agrospace_1109

Mićanović D, Hirsch P, Curnow P, Zečević V, Raičević V, Knežević D. Wheat colonization by determinate bacteria. in Savremena poljoprivreda. 2005;54(3-4):312-317.
https://hdl.handle.net/21.15107/rcub_agrospace_1109 .

Mićanović, Danica, Hirsch, Penny, Curnow, Phil, Zečević, Veselinka, Raičević, Vera, Knežević, Desimir, "Wheat colonization by determinate bacteria" in Savremena poljoprivreda, 54, no. 3-4 (2005):312-317,
https://hdl.handle.net/21.15107/rcub_agrospace_1109 .