Gašić, Uroš M.

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Authority KeyName Variants
orcid::0000-0001-5384-8396
  • Gašić, Uroš M. (26)
  • Gašić, Uroš (11)
Projects
Structure-properties relationships of natural and synthetic molecules and their metal complexes Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry)
Utilization of plant sources of protein, dietary fiber and antioxidants in food production Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200051 (Institute of General and Physical Chemistry, Belgrade) Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety
Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market FUNPRO - Functional products based on goat's milk proteins and bioactive compounds extracted from grape pomace and edible mushrooms
Research Council of NorwayResearch Council of Norway [280376] ENFIST Centre of Excellence infrastructure (TLC-MS interface)
Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade
Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan Evolution in Heterogeneous Environments: Adaptation Mechanisms, Biomonitoring and Conservation of Biodiversity
Molecular and physiological biomonitoring of aerobic organisms based on the determination of biochemical biomarkers of oxidative stress Society, spiritual and material culture and communications in the prehistory and early history of the Balkans
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200043 (Institute of Oncology and Radiology of Serbia, Belgrade)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200134 (University of Novi Sad, Faculty of Technology) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200169 (University of Belgrade, Faculty of Forestry)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200378 (Institute of Information Technology) Studying climate change and its influence on environment: impacts, adaptation and mitigation
Biotechnological approaches for overcoming effects of drought on agricultural production in Serbia Investigating the possibility of using contaminated waters for cultivation of pseudocereals

Author's Bibliography

Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties

Belošević, Spasoje; Milinčić, Danijel; Gašić, Uroš; Kostić, Aleksandar; Salević-Jelić, Ana; Marković, Jovana; Đorđević, Verica; Lević, Steva; Pešić, Mirjana; Nedović, Viktor

(2024)

TY  - JOUR
AU  - Belošević, Spasoje
AU  - Milinčić, Danijel
AU  - Gašić, Uroš
AU  - Kostić, Aleksandar
AU  - Salević-Jelić, Ana
AU  - Marković, Jovana
AU  - Đorđević, Verica
AU  - Lević, Steva
AU  - Pešić, Mirjana
AU  - Nedović, Viktor
PY  - 2024
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6895
AB  - The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.
T2  - Foods
T1  - Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties
IS  - 5
SP  - 757
VL  - 13
DO  - 10.3390/foods13050757
ER  - 
@article{
author = "Belošević, Spasoje and Milinčić, Danijel and Gašić, Uroš and Kostić, Aleksandar and Salević-Jelić, Ana and Marković, Jovana and Đorđević, Verica and Lević, Steva and Pešić, Mirjana and Nedović, Viktor",
year = "2024",
abstract = "The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.",
journal = "Foods",
title = "Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties",
number = "5",
pages = "757",
volume = "13",
doi = "10.3390/foods13050757"
}
Belošević, S., Milinčić, D., Gašić, U., Kostić, A., Salević-Jelić, A., Marković, J., Đorđević, V., Lević, S., Pešić, M.,& Nedović, V.. (2024). Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties. in Foods, 13(5), 757.
https://doi.org/10.3390/foods13050757
Belošević S, Milinčić D, Gašić U, Kostić A, Salević-Jelić A, Marković J, Đorđević V, Lević S, Pešić M, Nedović V. Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties. in Foods. 2024;13(5):757.
doi:10.3390/foods13050757 .
Belošević, Spasoje, Milinčić, Danijel, Gašić, Uroš, Kostić, Aleksandar, Salević-Jelić, Ana, Marković, Jovana, Đorđević, Verica, Lević, Steva, Pešić, Mirjana, Nedović, Viktor, "Broccoli, amaranth and red beet microgreens juices: the influence of cold-pressing on the phytochemical composition, antioxidant and sensory properties" in Foods, 13, no. 5 (2024):757,
https://doi.org/10.3390/foods13050757 . .

Biocompounds from mushroom aqueous and polysaccharide extracts

Milinčić, Danijel; Petrović, Jovana; Glamočlija, Jasmina; Gašić, Uroš; Doroški, Ana; Kostić, Aleksandar; Stanojević, Slađana; Pešić, Mirjana B.

(Srpsko hemijsko društvo, Karnegijeva 4, Beograd 11000, 2023)

TY  - CONF
AU  - Milinčić, Danijel
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Gašić, Uroš
AU  - Doroški, Ana
AU  - Kostić, Aleksandar
AU  - Stanojević, Slađana
AU  - Pešić, Mirjana B.
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6522
AB  - The application of mushrooms for medicinal purposes has a long history, primarily due to its therapeutic properties. Today,
mushrooms are often used as functional food or natural sources in the development of various nutraceuticals. Using advanced
instrumental techniques, it was shown that mushrooms are a good source of highly valuable polysaccharides (i.e.,
glucans), sterols (i.e., ergosterol), different antioxidants, proteins and peptides. However, due to the great diversity of fungi,
additional research in this area should be performed. The aim of this study is to analyze biocompounds from polysaccharides
and aqueous extracts of two different mushrooms (A. bisporus and A. aegerita). Mushroom extracts were prepared
according to procedure previously desribed by Popović Minić (2023)[1]. Lyophilised mushroom powder was extracted with
80% methanol containing 0.1% HCl, after which the suspension was filtered through 0.45μm filters and used for further
chromatographic analysis by UHPLC-QToF-MS. Chemical characterization of mushroom biomolecules was performed
using exact mass (m/z) and MS2 fragment ions of each detected compound and their retention times. The identified compounds
represented four structurally distinct groups: 1) organic acids and their derivatives (7 compounds); 2) phenolic
acids and their derivatives (11 compounds); 3) esters (28 compounds); and 4) other organic compounds (Gibberellin A1 ).
Based on the obtained results, the differences between the tested samples can be clearly observed. In A.bisposrus and
A.aegerita polysaccharide extracts only few organic acids and esters were detected, while phenolics and majority of esters
were not recorded. On the other hand, the presence of organic acids, phenolic acids, esters and their derivatives was confirmed
in both aqueous extracts. The highest number of detected compounds (as many as 41 compounds) was detected in
the aqueous extract of A. aegerita. Among organic acids, fumaric, malic and citric acids were detected in all the mushroom
extracts, whereas p-hydroxybenzoic acid, m-hydroxy-hydrocinnamic acid, sinapic acid, 2-(pentanoyloxy)benzoate, and
3-(11-hydroxyundecoxy) benzoate were detected among phenolic acids and their derivatives in aqueous extracts of both
mushrooms. Regarding detected esters, following compounds were identified in the tested samples: 8-carboxyoctanoate,
3-(octyloxy)-3-oxopropanoate, 9,12,13-trihydroxyoctadecenoate, 13-hydroxy-9,11-octadecadienoate. The estimated profiles
of biocompounds present in mushroom extracts can contribute to the further understanding of their antioxidant and
biological properties.
PB  - Srpsko hemijsko društvo, Karnegijeva 4, Beograd 11000
C3  - XXII Congress EuroFoodChem
T1  - Biocompounds from mushroom aqueous and polysaccharide extracts
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6522
ER  - 
@conference{
author = "Milinčić, Danijel and Petrović, Jovana and Glamočlija, Jasmina and Gašić, Uroš and Doroški, Ana and Kostić, Aleksandar and Stanojević, Slađana and Pešić, Mirjana B.",
year = "2023",
abstract = "The application of mushrooms for medicinal purposes has a long history, primarily due to its therapeutic properties. Today,
mushrooms are often used as functional food or natural sources in the development of various nutraceuticals. Using advanced
instrumental techniques, it was shown that mushrooms are a good source of highly valuable polysaccharides (i.e.,
glucans), sterols (i.e., ergosterol), different antioxidants, proteins and peptides. However, due to the great diversity of fungi,
additional research in this area should be performed. The aim of this study is to analyze biocompounds from polysaccharides
and aqueous extracts of two different mushrooms (A. bisporus and A. aegerita). Mushroom extracts were prepared
according to procedure previously desribed by Popović Minić (2023)[1]. Lyophilised mushroom powder was extracted with
80% methanol containing 0.1% HCl, after which the suspension was filtered through 0.45μm filters and used for further
chromatographic analysis by UHPLC-QToF-MS. Chemical characterization of mushroom biomolecules was performed
using exact mass (m/z) and MS2 fragment ions of each detected compound and their retention times. The identified compounds
represented four structurally distinct groups: 1) organic acids and their derivatives (7 compounds); 2) phenolic
acids and their derivatives (11 compounds); 3) esters (28 compounds); and 4) other organic compounds (Gibberellin A1 ).
Based on the obtained results, the differences between the tested samples can be clearly observed. In A.bisposrus and
A.aegerita polysaccharide extracts only few organic acids and esters were detected, while phenolics and majority of esters
were not recorded. On the other hand, the presence of organic acids, phenolic acids, esters and their derivatives was confirmed
in both aqueous extracts. The highest number of detected compounds (as many as 41 compounds) was detected in
the aqueous extract of A. aegerita. Among organic acids, fumaric, malic and citric acids were detected in all the mushroom
extracts, whereas p-hydroxybenzoic acid, m-hydroxy-hydrocinnamic acid, sinapic acid, 2-(pentanoyloxy)benzoate, and
3-(11-hydroxyundecoxy) benzoate were detected among phenolic acids and their derivatives in aqueous extracts of both
mushrooms. Regarding detected esters, following compounds were identified in the tested samples: 8-carboxyoctanoate,
3-(octyloxy)-3-oxopropanoate, 9,12,13-trihydroxyoctadecenoate, 13-hydroxy-9,11-octadecadienoate. The estimated profiles
of biocompounds present in mushroom extracts can contribute to the further understanding of their antioxidant and
biological properties.",
publisher = "Srpsko hemijsko društvo, Karnegijeva 4, Beograd 11000",
journal = "XXII Congress EuroFoodChem",
title = "Biocompounds from mushroom aqueous and polysaccharide extracts",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6522"
}
Milinčić, D., Petrović, J., Glamočlija, J., Gašić, U., Doroški, A., Kostić, A., Stanojević, S.,& Pešić, M. B.. (2023). Biocompounds from mushroom aqueous and polysaccharide extracts. in XXII Congress EuroFoodChem
Srpsko hemijsko društvo, Karnegijeva 4, Beograd 11000..
https://hdl.handle.net/21.15107/rcub_agrospace_6522
Milinčić D, Petrović J, Glamočlija J, Gašić U, Doroški A, Kostić A, Stanojević S, Pešić MB. Biocompounds from mushroom aqueous and polysaccharide extracts. in XXII Congress EuroFoodChem. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6522 .
Milinčić, Danijel, Petrović, Jovana, Glamočlija, Jasmina, Gašić, Uroš, Doroški, Ana, Kostić, Aleksandar, Stanojević, Slađana, Pešić, Mirjana B., "Biocompounds from mushroom aqueous and polysaccharide extracts" in XXII Congress EuroFoodChem (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6522 .

The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora

Fotirić Akšić, Milica; Mačukanović-Jocić, Marina; Radošević, Radenko; Nedić, Nebojša; Gašić, Uroš; Tosti, Tomislav; Tešić, Živoslav; Meland, Mekjell

(2023)

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Mačukanović-Jocić, Marina
AU  - Radošević, Radenko
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6338
AB  - The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance. © 2023 by the authors.
T2  - Horticulturae
T2  - Horticulturae
T1  - The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040424
ER  - 
@article{
author = "Fotirić Akšić, Milica and Mačukanović-Jocić, Marina and Radošević, Radenko and Nedić, Nebojša and Gašić, Uroš and Tosti, Tomislav and Tešić, Živoslav and Meland, Mekjell",
year = "2023",
abstract = "The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance. © 2023 by the authors.",
journal = "Horticulturae, Horticulturae",
title = "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040424"
}
Fotirić Akšić, M., Mačukanović-Jocić, M., Radošević, R., Nedić, N., Gašić, U., Tosti, T., Tešić, Ž.,& Meland, M.. (2023). The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae, 9(4).
https://doi.org/10.3390/horticulturae9040424
Fotirić Akšić M, Mačukanović-Jocić M, Radošević R, Nedić N, Gašić U, Tosti T, Tešić Ž, Meland M. The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae. 2023;9(4).
doi:10.3390/horticulturae9040424 .
Fotirić Akšić, Milica, Mačukanović-Jocić, Marina, Radošević, Radenko, Nedić, Nebojša, Gašić, Uroš, Tosti, Tomislav, Tešić, Živoslav, Meland, Mekjell, "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora" in Horticulturae, 9, no. 4 (2023),
https://doi.org/10.3390/horticulturae9040424 . .
2

Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway

Fotirić Akšić, Milica; Tešić, Živoslav; Kalaba, Milica; Ćirić, Ivanka; Pezo, Lato; Lončar, Biljana; Gašić, Uroš; Dojčinović, Biljana; Tosti, Tomislav; Meland, Mekjell

(2023)

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6340
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds. © 2023 by the authors.
T2  - Horticulturae
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
ER  - 
@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds. © 2023 by the authors.",
journal = "Horticulturae, Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477"
}
Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae, 9(4).
https://doi.org/10.3390/horticulturae9040477
Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4).
doi:10.3390/horticulturae9040477 .
Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023),
https://doi.org/10.3390/horticulturae9040477 . .
2
1

Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region

Jabboury, Zineb El; Ousaaid, Driss; Gašić, Uroš; Janaćković, Peđa; Stevanovic, Zora Dajic; Kolašinac, Stefan; Benjelloun, Meryem; Ghadraoui, Lahsen El

(2023)

TY  - JOUR
AU  - Jabboury, Zineb El
AU  - Ousaaid, Driss
AU  - Gašić, Uroš
AU  - Janaćković, Peđa
AU  - Stevanovic, Zora Dajic
AU  - Kolašinac, Stefan
AU  - Benjelloun, Meryem
AU  - Ghadraoui, Lahsen El
PY  - 2023
UR  - https://doi.org/10.1007/s42250-023-00747-8
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6416
AB  - Ammi visnaga is a very popular medicinal plant well known for its biological properties afforded by its dense chemical composition highly dependent on environmental factors. The present study was designed to examine the antioxidant potencies using four complementary assays (Total phenolic content (TPC), total antioxidant capacity (TAC), dihydroxycinnamic acid derivative content (HCA), and cupric ion reducing antioxidant capacity (CUPRAC)) as well as the phenolic profile of different parts of Moroccan Ammi visnaga. The obtained results showed that the flower extract registered the highest amount of TPC (49,71 ± 0,12 mg GAE/g) and antioxidant activity TAC (62.07 ± 2.98 mg AAE/g). Concerning the phenolic profile, the most abundant individual phenolic compounds found in high amounts are chlorogenic acid (56.03%, 11.23% and 32.29 for stem, leaves and flowers, respectively), isorhamnetin_3-O-rutinoside (17.86%, 12.16% and 19.01% for stem, leaves and flowers, respectively), isorhamnetin_3-O-glucoside (15.96%, 10.69%, 10.37% for stem, leaves and flowers, respectively), quercetin_3-O-glucoside (1.61%, 21.11% and 2.85% for stem, leaves and flowers, respectively), kaempferol_3-O-glucoside (1.04%, 3.17% and 7.40% for stem, leaves and flowers, respectively) and isorhamnetin (0.57% 7.59% and 2.77% for stem, leaves and flowers, respectively). The outcomes of this study showed that different parts of A. visnaga contain several bioactive compounds with unique biological properties, so the data required further validation using experimental models.
T2  - Chemistry Africa
T2  - Chemistry AfricaChemistry Africa
T1  - Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region
DO  - 10.1007/s42250-023-00747-8
ER  - 
@article{
author = "Jabboury, Zineb El and Ousaaid, Driss and Gašić, Uroš and Janaćković, Peđa and Stevanovic, Zora Dajic and Kolašinac, Stefan and Benjelloun, Meryem and Ghadraoui, Lahsen El",
year = "2023",
abstract = "Ammi visnaga is a very popular medicinal plant well known for its biological properties afforded by its dense chemical composition highly dependent on environmental factors. The present study was designed to examine the antioxidant potencies using four complementary assays (Total phenolic content (TPC), total antioxidant capacity (TAC), dihydroxycinnamic acid derivative content (HCA), and cupric ion reducing antioxidant capacity (CUPRAC)) as well as the phenolic profile of different parts of Moroccan Ammi visnaga. The obtained results showed that the flower extract registered the highest amount of TPC (49,71 ± 0,12 mg GAE/g) and antioxidant activity TAC (62.07 ± 2.98 mg AAE/g). Concerning the phenolic profile, the most abundant individual phenolic compounds found in high amounts are chlorogenic acid (56.03%, 11.23% and 32.29 for stem, leaves and flowers, respectively), isorhamnetin_3-O-rutinoside (17.86%, 12.16% and 19.01% for stem, leaves and flowers, respectively), isorhamnetin_3-O-glucoside (15.96%, 10.69%, 10.37% for stem, leaves and flowers, respectively), quercetin_3-O-glucoside (1.61%, 21.11% and 2.85% for stem, leaves and flowers, respectively), kaempferol_3-O-glucoside (1.04%, 3.17% and 7.40% for stem, leaves and flowers, respectively) and isorhamnetin (0.57% 7.59% and 2.77% for stem, leaves and flowers, respectively). The outcomes of this study showed that different parts of A. visnaga contain several bioactive compounds with unique biological properties, so the data required further validation using experimental models.",
journal = "Chemistry Africa, Chemistry AfricaChemistry Africa",
title = "Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region",
doi = "10.1007/s42250-023-00747-8"
}
Jabboury, Z. E., Ousaaid, D., Gašić, U., Janaćković, P., Stevanovic, Z. D., Kolašinac, S., Benjelloun, M.,& Ghadraoui, L. E.. (2023). Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region. in Chemistry Africa.
https://doi.org/10.1007/s42250-023-00747-8
Jabboury ZE, Ousaaid D, Gašić U, Janaćković P, Stevanovic ZD, Kolašinac S, Benjelloun M, Ghadraoui LE. Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region. in Chemistry Africa. 2023;.
doi:10.1007/s42250-023-00747-8 .
Jabboury, Zineb El, Ousaaid, Driss, Gašić, Uroš, Janaćković, Peđa, Stevanovic, Zora Dajic, Kolašinac, Stefan, Benjelloun, Meryem, Ghadraoui, Lahsen El, "Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region" in Chemistry Africa (2023),
https://doi.org/10.1007/s42250-023-00747-8 . .
2

Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia

Katanić Stanković, Jelena S.; Mićanović, Nenad; Grozdanić, Nadja; Kostić, Aleksandar Ž; Gašić, Uroš; Stanojković, Tatjana; Popović-Djordjević, Jelena B.

(2022)

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Mićanović, Nenad
AU  - Grozdanić, Nadja
AU  - Kostić, Aleksandar Ž
AU  - Gašić, Uroš
AU  - Stanojković, Tatjana
AU  - Popović-Djordjević, Jelena B.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/11/1053
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6387
AB  - Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.
T2  - Horticulturae
T2  - Horticulturae
T1  - Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia
IS  - 11
SP  - 1053
VL  - 8
DO  - 10.3390/horticulturae8111053
ER  - 
@article{
author = "Katanić Stanković, Jelena S. and Mićanović, Nenad and Grozdanić, Nadja and Kostić, Aleksandar Ž and Gašić, Uroš and Stanojković, Tatjana and Popović-Djordjević, Jelena B.",
year = "2022",
abstract = "Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.",
journal = "Horticulturae, Horticulturae",
title = "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia",
number = "11",
pages = "1053",
volume = "8",
doi = "10.3390/horticulturae8111053"
}
Katanić Stanković, J. S., Mićanović, N., Grozdanić, N., Kostić, A. Ž., Gašić, U., Stanojković, T.,& Popović-Djordjević, J. B.. (2022). Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae, 8(11), 1053.
https://doi.org/10.3390/horticulturae8111053
Katanić Stanković JS, Mićanović N, Grozdanić N, Kostić AŽ, Gašić U, Stanojković T, Popović-Djordjević JB. Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae. 2022;8(11):1053.
doi:10.3390/horticulturae8111053 .
Katanić Stanković, Jelena S., Mićanović, Nenad, Grozdanić, Nadja, Kostić, Aleksandar Ž, Gašić, Uroš, Stanojković, Tatjana, Popović-Djordjević, Jelena B., "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia" in Horticulturae, 8, no. 11 (2022):1053,
https://doi.org/10.3390/horticulturae8111053 . .
1
12

Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)

Ćirković, Dušica; Matijašević, Saša; Ćirković, Bratislav; Laketić, Darko; Jovanović, Zoran; Kostić, Boban; Bešlić, Zoran; Sredojević, Milica; Tešić, Živoslav; Banjanac, Tijana; Gašić, Uroš

(MDPI, 2022)

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Ćirković, Bratislav
AU  - Laketić, Darko
AU  - Jovanović, Zoran
AU  - Kostić, Boban
AU  - Bešlić, Zoran
AU  - Sredojević, Milica
AU  - Tešić, Živoslav
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6070
AB  - The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation— treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.
PB  - MDPI
T2  - Horticulturae
T1  - Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)
IS  - 4
SP  - 296
VL  - 8
DO  - 10.3390/horticulturae8040296
ER  - 
@article{
author = "Ćirković, Dušica and Matijašević, Saša and Ćirković, Bratislav and Laketić, Darko and Jovanović, Zoran and Kostić, Boban and Bešlić, Zoran and Sredojević, Milica and Tešić, Živoslav and Banjanac, Tijana and Gašić, Uroš",
year = "2022",
abstract = "The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation— treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)",
number = "4",
pages = "296",
volume = "8",
doi = "10.3390/horticulturae8040296"
}
Ćirković, D., Matijašević, S., Ćirković, B., Laketić, D., Jovanović, Z., Kostić, B., Bešlić, Z., Sredojević, M., Tešić, Ž., Banjanac, T.,& Gašić, U.. (2022). Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae
MDPI., 8(4), 296.
https://doi.org/10.3390/horticulturae8040296
Ćirković D, Matijašević S, Ćirković B, Laketić D, Jovanović Z, Kostić B, Bešlić Z, Sredojević M, Tešić Ž, Banjanac T, Gašić U. Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae. 2022;8(4):296.
doi:10.3390/horticulturae8040296 .
Ćirković, Dušica, Matijašević, Saša, Ćirković, Bratislav, Laketić, Darko, Jovanović, Zoran, Kostić, Boban, Bešlić, Zoran, Sredojević, Milica, Tešić, Živoslav, Banjanac, Tijana, Gašić, Uroš, "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)" in Horticulturae, 8, no. 4 (2022):296,
https://doi.org/10.3390/horticulturae8040296 . .
1
3
2

Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems

Fotirić Akšić, Milica; Dabić Zagorac, Dragana; Gašić, Uroš; Tosti, Tomislav; Natić, Maja; Meland, Mekjell

(2022)

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Dabić Zagorac, Dragana
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Natić, Maja
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.mdpi.com/2071-1050/14/9/5300
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6094
AB  - The aim of this study was to compare total phenolic content (TPC), radical-scavenging activity (RSA), total anthocyanin content (TAC), sugar and polyphenolic profiles of two apple cultivars (‘Discovery’ and ‘Red Aroma Orelind’) from organic and integrated production systems in climatic conditions of Western Norway. Sixteen sugars and four sugar alcohols and 19 polyphenols were found in the peel, but less polyphenols were detected in the pulp. The peel of both apples and in both production systems had significantly higher TPC and RSA than the pulp. The peel from integrated apples had higher TPC than the peel from organic apples, while organic apples had higher TAC than the integrated. Sucrose and glucose levels were higher in organic apples; fructose was cultivar dependent while minor sugars were higher in integrated fruits. The most abundant polyphenolic compound in the peel of the tested cultivars was quercetin 3-O-galactoside, while chlorogenic acid was most abundant in the pulp. Regarding polyphenols, phloretin, phloridzin, protocatechuic acid, baicalein and naringenin were higher in organic apple, while quercetin 3-O-galactoside, kaempferol 3-O-glucoside, chlorogenic acid and syringic acid was higher in integrated fruits. In conclusion, organic ‘Discovery’ and integrated ‘Red Aroma Orelind’ had higher bioavailability of health related compounds from the peel and the pulp.
T2  - Sustainability
T2  - Sustainability
T1  - Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems
IS  - 9
SP  - 5300
VL  - 14
DO  - 10.3390/su14095300
ER  - 
@article{
author = "Fotirić Akšić, Milica and Dabić Zagorac, Dragana and Gašić, Uroš and Tosti, Tomislav and Natić, Maja and Meland, Mekjell",
year = "2022",
abstract = "The aim of this study was to compare total phenolic content (TPC), radical-scavenging activity (RSA), total anthocyanin content (TAC), sugar and polyphenolic profiles of two apple cultivars (‘Discovery’ and ‘Red Aroma Orelind’) from organic and integrated production systems in climatic conditions of Western Norway. Sixteen sugars and four sugar alcohols and 19 polyphenols were found in the peel, but less polyphenols were detected in the pulp. The peel of both apples and in both production systems had significantly higher TPC and RSA than the pulp. The peel from integrated apples had higher TPC than the peel from organic apples, while organic apples had higher TAC than the integrated. Sucrose and glucose levels were higher in organic apples; fructose was cultivar dependent while minor sugars were higher in integrated fruits. The most abundant polyphenolic compound in the peel of the tested cultivars was quercetin 3-O-galactoside, while chlorogenic acid was most abundant in the pulp. Regarding polyphenols, phloretin, phloridzin, protocatechuic acid, baicalein and naringenin were higher in organic apple, while quercetin 3-O-galactoside, kaempferol 3-O-glucoside, chlorogenic acid and syringic acid was higher in integrated fruits. In conclusion, organic ‘Discovery’ and integrated ‘Red Aroma Orelind’ had higher bioavailability of health related compounds from the peel and the pulp.",
journal = "Sustainability, Sustainability",
title = "Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems",
number = "9",
pages = "5300",
volume = "14",
doi = "10.3390/su14095300"
}
Fotirić Akšić, M., Dabić Zagorac, D., Gašić, U., Tosti, T., Natić, M.,& Meland, M.. (2022). Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems. in Sustainability, 14(9), 5300.
https://doi.org/10.3390/su14095300
Fotirić Akšić M, Dabić Zagorac D, Gašić U, Tosti T, Natić M, Meland M. Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems. in Sustainability. 2022;14(9):5300.
doi:10.3390/su14095300 .
Fotirić Akšić, Milica, Dabić Zagorac, Dragana, Gašić, Uroš, Tosti, Tomislav, Natić, Maja, Meland, Mekjell, "Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems" in Sustainability, 14, no. 9 (2022):5300,
https://doi.org/10.3390/su14095300 . .
1
18
20

Honey with added value – enriched with rutin and quercetin from Sophora flower

Đorđević, Sofija; Nedić, Nebojša; Pavlović, Aleksandra; Milojković-Opsenica, Dušanka; Tešić, Živoslav; Gašić, Uroš

(2022)

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S2210803322000495
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6114
AB  - IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
SP  - 100580
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
ER  - 
@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
pages = "100580",
volume = "34",
doi = "10.1016/j.hermed.2022.100580"
}
Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580
Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .
Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .
3

Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties

Milinčić, Danijel D.; Kostić, Aleksandar Ž.; Gašić, Uroš M.; Lević, Steva; Stanojević, Sladjana P.; Barać, Miroljub B.; Tešić, Živoslav Lj.; Nedović, Viktor; Pešić, Mirjana B.

(MDPI AG, 2021)

TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš M.
AU  - Lević, Steva
AU  - Stanojević, Sladjana P.
AU  - Barać, Miroljub B.
AU  - Tešić, Živoslav Lj.
AU  - Nedović, Viktor
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5882
AB  - The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.
PB  - MDPI AG
T2  - Biomolecules
T1  - Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties
IS  - 7
SP  - 965
VL  - 11
DO  - 10.3390/biom11070965
ER  - 
@article{
author = "Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Gašić, Uroš M. and Lević, Steva and Stanojević, Sladjana P. and Barać, Miroljub B. and Tešić, Živoslav Lj. and Nedović, Viktor and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties",
number = "7",
pages = "965",
volume = "11",
doi = "10.3390/biom11070965"
}
Milinčić, D. D., Kostić, A. Ž., Gašić, U. M., Lević, S., Stanojević, S. P., Barać, M. B., Tešić, Ž. Lj., Nedović, V.,& Pešić, M. B.. (2021). Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules
MDPI AG., 11(7), 965.
https://doi.org/10.3390/biom11070965
Milinčić DD, Kostić AŽ, Gašić UM, Lević S, Stanojević SP, Barać MB, Tešić ŽL, Nedović V, Pešić MB. Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules. 2021;11(7):965.
doi:10.3390/biom11070965 .
Milinčić, Danijel D., Kostić, Aleksandar Ž., Gašić, Uroš M., Lević, Steva, Stanojević, Sladjana P., Barać, Miroljub B., Tešić, Živoslav Lj., Nedović, Viktor, Pešić, Mirjana B., "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties" in Biomolecules, 11, no. 7 (2021):965,
https://doi.org/10.3390/biom11070965 . .
11
2
10

Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Nedić, Nebojša; Gašić, Uroš M.; Špirović Trifunović, Bojana; Vojt, Denis; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(MDPI AG, 2021)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5886
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI AG
T2  - Antioxidants
T1  - Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen
IS  - 7
SP  - 1091
VL  - 10
DO  - 10.3390/antiox10071091
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen",
number = "7",
pages = "1091",
volume = "10",
doi = "10.3390/antiox10071091"
}
Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants
MDPI AG., 10(7), 1091.
https://doi.org/10.3390/antiox10071091
Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .
22
2
18

Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Kolarević, Tijana; Milinčić, Danijel D.; Vujović, Tatjana; Gašić, Uroš M.; Prokić, Ljiljana; Kostić, Aleksandar Ž.; Cerović, Radosav; Stanojević, Sladjana P.; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(MDPI, 2021)

TY  - JOUR
AU  - Kolarević, Tijana
AU  - Milinčić, Danijel D.
AU  - Vujović, Tatjana
AU  - Gašić, Uroš M.
AU  - Prokić, Ljiljana
AU  - Kostić, Aleksandar Ž.
AU  - Cerović, Radosav
AU  - Stanojević, Sladjana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5958
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
PB  - MDPI
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
SP  - 420
VL  - 7
DO  - 10.3390/horticulturae7110420
ER  - 
@article{
author = "Kolarević, Tijana and Milinčić, Danijel D. and Vujović, Tatjana and Gašić, Uroš M. and Prokić, Ljiljana and Kostić, Aleksandar Ž. and Cerović, Radosav and Stanojević, Sladjana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
pages = "420",
volume = "7",
doi = "10.3390/horticulturae7110420"
}
Kolarević, T., Milinčić, D. D., Vujović, T., Gašić, U. M., Prokić, L., Kostić, A. Ž., Cerović, R., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae
MDPI., 7(11), 420.
https://doi.org/10.3390/horticulturae7110420
Kolarević T, Milinčić DD, Vujović T, Gašić UM, Prokić L, Kostić AŽ, Cerović R, Stanojević SP, Tešić ŽL, Pešić MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11):420.
doi:10.3390/horticulturae7110420 .
Kolarević, Tijana, Milinčić, Danijel D., Vujović, Tatjana, Gašić, Uroš M., Prokić, Ljiljana, Kostić, Aleksandar Ž., Cerović, Radosav, Stanojević, Sladjana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021):420,
https://doi.org/10.3390/horticulturae7110420 . .
1
12
1
10

Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Stanisavljević, Nemanja S.; Gašić, Uroš M.; Lević, Steva; Kojić, Milan O.; Tešić, Živoslav Lj.; Nedović, Viktor; Barać, Miroljub B.; Pešić, Mirjana B.

(Elsevier Ltd, 2021)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Stanisavljević, Nemanja S.
AU  - Gašić, Uroš M.
AU  - Lević, Steva
AU  - Kojić, Milan O.
AU  - Tešić, Živoslav Lj.
AU  - Nedović, Viktor
AU  - Barać, Miroljub B.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5814
AB  - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen
SP  - 129310
VL  - 351
DO  - 10.1016/j.foodchem.2021.129310
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Gašić, Uroš M. and Lević, Steva and Kojić, Milan O. and Tešić, Živoslav Lj. and Nedović, Viktor and Barać, Miroljub B. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen",
pages = "129310",
volume = "351",
doi = "10.1016/j.foodchem.2021.129310"
}
Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U. M., Lević, S., Kojić, M. O., Tešić, Ž. Lj., Nedović, V., Barać, M. B.,& Pešić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry
Elsevier Ltd., 351, 129310.
https://doi.org/10.1016/j.foodchem.2021.129310
Kostić AŽ, Milinčić DD, Stanisavljević NS, Gašić UM, Lević S, Kojić MO, Tešić ŽL, Nedović V, Barać MB, Pešić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry. 2021;351:129310.
doi:10.1016/j.foodchem.2021.129310 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Stanisavljević, Nemanja S., Gašić, Uroš M., Lević, Steva, Kojić, Milan O., Tešić, Živoslav Lj., Nedović, Viktor, Barać, Miroljub B., Pešić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen" in Food Chemistry, 351 (2021):129310,
https://doi.org/10.1016/j.foodchem.2021.129310 . .
38
5
34

Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries

Skočajić, Dragana; Gašić, Uroš; Dabić Zagorac, Dragana; Nešić, Marija; Tešić, Živoslav; Meland, Mekjell; Fotirić-Akšić, Milica

(MDPI, 2021)

TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gašić, Uroš
AU  - Dabić Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
AU  - Fotirić-Akšić, Milica
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6005
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars (‘Amanogawa’, ‘Kanzan’, and ‘Kiku-shidare-zakura’) and commercial rootstocks. Prunus avium, Prunus ‘Colt’, Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis ‘Pyrodwarf’ were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with ‘Amanogawa’ and ‘Kiku-shidare-zakura’ the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
PB  - MDPI
T2  - Plants
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries
IS  - 12
SP  - 2822
VL  - 10
DO  - 10.3390/plants10122822
ER  - 
@article{
author = "Skočajić, Dragana and Gašić, Uroš and Dabić Zagorac, Dragana and Nešić, Marija and Tešić, Živoslav and Meland, Mekjell and Fotirić-Akšić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars (‘Amanogawa’, ‘Kanzan’, and ‘Kiku-shidare-zakura’) and commercial rootstocks. Prunus avium, Prunus ‘Colt’, Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis ‘Pyrodwarf’ were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with ‘Amanogawa’ and ‘Kiku-shidare-zakura’ the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
publisher = "MDPI",
journal = "Plants",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries",
number = "12",
pages = "2822",
volume = "10",
doi = "10.3390/plants10122822"
}
Skočajić, D., Gašić, U., Dabić Zagorac, D., Nešić, M., Tešić, Ž., Meland, M.,& Fotirić-Akšić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants
MDPI., 10(12), 2822.
https://doi.org/10.3390/plants10122822
Skočajić D, Gašić U, Dabić Zagorac D, Nešić M, Tešić Ž, Meland M, Fotirić-Akšić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants. 2021;10(12):2822.
doi:10.3390/plants10122822 .
Skočajić, Dragana, Gašić, Uroš, Dabić Zagorac, Dragana, Nešić, Marija, Tešić, Živoslav, Meland, Mekjell, Fotirić-Akšić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries" in Plants, 10, no. 12 (2021):2822,
https://doi.org/10.3390/plants10122822 . .
1
1

Distribution of polyphenolic and sugar compounds in different buckwheat plant parts

Nešović, Milica; Gašić, Uroš; Tosti, Tomislav; Horvacki, Nikola; Nedić, Nebojša; Sredojević, Milica; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav

(Royal Society of Chemistry, 2021)

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5911
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
EP  - 25829
IS  - 42
SP  - 25816
VL  - 11
DO  - 10.1039/d1ra04250e
ER  - 
@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
pages = "25829-25816",
number = "42",
volume = "11",
doi = "10.1039/d1ra04250e"
}
Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/d1ra04250e
Nešović M, Gašić U, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić Ž. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/d1ra04250e .
Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/d1ra04250e . .
1
26
1
22

Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars

Stikić, Radmila; Milincić, Danijel D.; Kostić, Aleksandar; Jovanović, Zorica; Gašić, Uroš M.; Tešić, Živoslav; Djordjević, Nataša Z.; Savić, Sladjana; Czekus, Borisz G.; Pešić, Mirjana

(Wiley, Hoboken, 2020)

TY  - JOUR
AU  - Stikić, Radmila
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Jovanović, Zorica
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5367
AB  - BACKGROUND:  and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.
PB  - Wiley, Hoboken
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
EP  - 633
IS  - 3
SP  - 626
VL  - 97
DO  - 10.1002/cche.10278
ER  - 
@article{
author = "Stikić, Radmila and Milincić, Danijel D. and Kostić, Aleksandar and Jovanović, Zorica and Gašić, Uroš M. and Tešić, Živoslav and Djordjević, Nataša Z. and Savić, Sladjana and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "BACKGROUND:  and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.",
publisher = "Wiley, Hoboken",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
pages = "633-626",
number = "3",
volume = "97",
doi = "10.1002/cche.10278"
}
Stikić, R., Milincić, D. D., Kostić, A., Jovanović, Z., Gašić, U. M., Tešić, Ž., Djordjević, N. Z., Savić, S., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry
Wiley, Hoboken., 97(3), 626-633.
https://doi.org/10.1002/cche.10278
Stikić R, Milincić DD, Kostić A, Jovanović Z, Gašić UM, Tešić Ž, Djordjević NZ, Savić S, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):626-633.
doi:10.1002/cche.10278 .
Stikić, Radmila, Milincić, Danijel D., Kostić, Aleksandar, Jovanović, Zorica, Gašić, Uroš M., Tešić, Živoslav, Djordjević, Nataša Z., Savić, Sladjana, Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):626-633,
https://doi.org/10.1002/cche.10278 . .
1
24
8
22

Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species

Dabić-Zagorac, Dragana; Fotirić-Akšić, Milica; Glavnik, Vesna; Gašić, Uroš M.; Vovk, Irena; Tešić, Živoslav; Natić, Maja

(Wiley-V C H Verlag Gmbh, Weinheim, 2020)

TY  - JOUR
AU  - Dabić-Zagorac, Dragana
AU  - Fotirić-Akšić, Milica
AU  - Glavnik, Vesna
AU  - Gašić, Uroš M.
AU  - Vovk, Irena
AU  - Tešić, Živoslav
AU  - Natić, Maja
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5408
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimerwere identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
EP  - 1439
IS  - 8
SP  - 1431
VL  - 43
DO  - 10.1002/jssc.201901271
ER  - 
@article{
author = "Dabić-Zagorac, Dragana and Fotirić-Akšić, Milica and Glavnik, Vesna and Gašić, Uroš M. and Vovk, Irena and Tešić, Živoslav and Natić, Maja",
year = "2020",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimerwere identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
pages = "1439-1431",
number = "8",
volume = "43",
doi = "10.1002/jssc.201901271"
}
Dabić-Zagorac, D., Fotirić-Akšić, M., Glavnik, V., Gašić, U. M., Vovk, I., Tešić, Ž.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science
Wiley-V C H Verlag Gmbh, Weinheim., 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271
Dabić-Zagorac D, Fotirić-Akšić M, Glavnik V, Gašić UM, Vovk I, Tešić Ž, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .
Dabić-Zagorac, Dragana, Fotirić-Akšić, Milica, Glavnik, Vesna, Gašić, Uroš M., Vovk, Irena, Tešić, Živoslav, Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .
1
12
3
10

Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro

Suković, Danijela; Knežević, Bojana; Gašić, Uroš M.; Sredojević, Milica; Ćirić, Ivanka; Todić, Slavica; Mutić, Jelena; Tešić, Živoslav

(MDPI, BASEL, 2020)

TY  - JOUR
AU  - Suković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš M.
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5391
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
PB  - MDPI, BASEL
T2  - Foods
T1  - Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro
IS  - 2
VL  - 9
DO  - 10.3390/foods9020138
ER  - 
@article{
author = "Suković, Danijela and Knežević, Bojana and Gašić, Uroš M. and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav",
year = "2020",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
publisher = "MDPI, BASEL",
journal = "Foods",
title = "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro",
number = "2",
volume = "9",
doi = "10.3390/foods9020138"
}
Suković, D., Knežević, B., Gašić, U. M., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž.. (2020). Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro. in Foods
MDPI, BASEL., 9(2).
https://doi.org/10.3390/foods9020138
Suković D, Knežević B, Gašić UM, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić Ž. Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro. in Foods. 2020;9(2).
doi:10.3390/foods9020138 .
Suković, Danijela, Knežević, Bojana, Gašić, Uroš M., Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav, "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro" in Foods, 9, no. 2 (2020),
https://doi.org/10.3390/foods9020138 . .
1
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17
64

Influence of rootstocks on the chemical composition of the fruits of plum cultivars

Radović, Mirjana; Milatović, Dragan; Tešić, Živoslav; Tosti, Tomislav; Gašić, Uroš M.; Dojčinović, Biljana; Dabić-Zagorac, Dragana

(Academic Press Inc Elsevier Science, San Diego, 2020)

TY  - JOUR
AU  - Radović, Mirjana
AU  - Milatović, Dragan
AU  - Tešić, Živoslav
AU  - Tosti, Tomislav
AU  - Gašić, Uroš M.
AU  - Dojčinović, Biljana
AU  - Dabić-Zagorac, Dragana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5247
AB  - This paper presents the chemical composition of the fruits of three European plum cultivars ('Cacanska Rana', 'Cacanska Lepotica', and 'Cacanska Najbolja') grafted on four rootstocks (Myrobalan, 'Pixy', Fereley', and 'St Julien A'). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g(-1) fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg(-1), while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2-147 mg g(-1). The highest glucose and fructose contents were in `Cacanska Lepotica', then in `Cacanska Najbolja', and the lowest were in `Cacanska Rana' cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on 'St Julien A' rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Influence of rootstocks on the chemical composition of the fruits of plum cultivars
VL  - 92
DO  - 10.1016/j.jfca.2020.103480
ER  - 
@article{
author = "Radović, Mirjana and Milatović, Dragan and Tešić, Živoslav and Tosti, Tomislav and Gašić, Uroš M. and Dojčinović, Biljana and Dabić-Zagorac, Dragana",
year = "2020",
abstract = "This paper presents the chemical composition of the fruits of three European plum cultivars ('Cacanska Rana', 'Cacanska Lepotica', and 'Cacanska Najbolja') grafted on four rootstocks (Myrobalan, 'Pixy', Fereley', and 'St Julien A'). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g(-1) fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg(-1), while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2-147 mg g(-1). The highest glucose and fructose contents were in `Cacanska Lepotica', then in `Cacanska Najbolja', and the lowest were in `Cacanska Rana' cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on 'St Julien A' rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Influence of rootstocks on the chemical composition of the fruits of plum cultivars",
volume = "92",
doi = "10.1016/j.jfca.2020.103480"
}
Radović, M., Milatović, D., Tešić, Ž., Tosti, T., Gašić, U. M., Dojčinović, B.,& Dabić-Zagorac, D.. (2020). Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 92.
https://doi.org/10.1016/j.jfca.2020.103480
Radović M, Milatović D, Tešić Ž, Tosti T, Gašić UM, Dojčinović B, Dabić-Zagorac D. Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis. 2020;92.
doi:10.1016/j.jfca.2020.103480 .
Radović, Mirjana, Milatović, Dragan, Tešić, Živoslav, Tosti, Tomislav, Gašić, Uroš M., Dojčinović, Biljana, Dabić-Zagorac, Dragana, "Influence of rootstocks on the chemical composition of the fruits of plum cultivars" in Journal of Food Composition and Analysis, 92 (2020),
https://doi.org/10.1016/j.jfca.2020.103480 . .
28
9
28

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species

Kostić, Aleksandar; Gašić, Uroš M.; Pešić, Mirjana; Stanojević, Sladjana; Barać, Miroljub; Mačukanović-Jocić, Marina; Avramov, Stevan N.; Tešić, Živoslav

(Wiley-V C H Verlag Gmbh, Weinheim, 2019)

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš M.
AU  - Pešić, Mirjana
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Mačukanović-Jocić, Marina
AU  - Avramov, Stevan N.
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5116
AB  - This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above-ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC-Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C-glycosides, luteolin O-glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species
IS  - 3
VL  - 16
DO  - 10.1002/cbdv.201800565
ER  - 
@article{
author = "Kostić, Aleksandar and Gašić, Uroš M. and Pešić, Mirjana and Stanojević, Sladjana and Barać, Miroljub and Mačukanović-Jocić, Marina and Avramov, Stevan N. and Tešić, Živoslav",
year = "2019",
abstract = "This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above-ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC-Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C-glycosides, luteolin O-glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species",
number = "3",
volume = "16",
doi = "10.1002/cbdv.201800565"
}
Kostić, A., Gašić, U. M., Pešić, M., Stanojević, S., Barać, M., Mačukanović-Jocić, M., Avramov, S. N.,& Tešić, Ž.. (2019). Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 16(3).
https://doi.org/10.1002/cbdv.201800565
Kostić A, Gašić UM, Pešić M, Stanojević S, Barać M, Mačukanović-Jocić M, Avramov SN, Tešić Ž. Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity. 2019;16(3).
doi:10.1002/cbdv.201800565 .
Kostić, Aleksandar, Gašić, Uroš M., Pešić, Mirjana, Stanojević, Sladjana, Barać, Miroljub, Mačukanović-Jocić, Marina, Avramov, Stevan N., Tešić, Živoslav, "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species" in Chemistry & Biodiversity, 16, no. 3 (2019),
https://doi.org/10.1002/cbdv.201800565 . .
1
33
15
30

In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?

Pešić, Mirjana; Milincić, Danijel D.; Kostić, Aleksandar; Stanisavljević, Nemanja S.; Vukotić, Goran; Kojić, Milan; Gašić, Uroš M.; Barać, Miroljub; Stanojević, Sladjana; Popović, Dušanka A.; Banjac, Nebojša; Tešić, Živoslav

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Stanisavljević, Nemanja S.
AU  - Vukotić, Goran
AU  - Kojić, Milan
AU  - Gašić, Uroš M.
AU  - Barać, Miroljub
AU  - Stanojević, Sladjana
AU  - Popović, Dušanka A.
AU  - Banjac, Nebojša
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5034
AB  - The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?
EP  - 44
SP  - 28
VL  - 284
DO  - 10.1016/j.foodchem.2019.01.107
ER  - 
@article{
author = "Pešić, Mirjana and Milincić, Danijel D. and Kostić, Aleksandar and Stanisavljević, Nemanja S. and Vukotić, Goran and Kojić, Milan and Gašić, Uroš M. and Barać, Miroljub and Stanojević, Sladjana and Popović, Dušanka A. and Banjac, Nebojša and Tešić, Živoslav",
year = "2019",
abstract = "The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?",
pages = "44-28",
volume = "284",
doi = "10.1016/j.foodchem.2019.01.107"
}
Pešić, M., Milincić, D. D., Kostić, A., Stanisavljević, N. S., Vukotić, G., Kojić, M., Gašić, U. M., Barać, M., Stanojević, S., Popović, D. A., Banjac, N.,& Tešić, Ž.. (2019). In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry
Elsevier Sci Ltd, Oxford., 284, 28-44.
https://doi.org/10.1016/j.foodchem.2019.01.107
Pešić M, Milincić DD, Kostić A, Stanisavljević NS, Vukotić G, Kojić M, Gašić UM, Barać M, Stanojević S, Popović DA, Banjac N, Tešić Ž. In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry. 2019;284:28-44.
doi:10.1016/j.foodchem.2019.01.107 .
Pešić, Mirjana, Milincić, Danijel D., Kostić, Aleksandar, Stanisavljević, Nemanja S., Vukotić, Goran, Kojić, Milan, Gašić, Uroš M., Barać, Miroljub, Stanojević, Sladjana, Popović, Dušanka A., Banjac, Nebojša, Tešić, Živoslav, "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?" in Food Chemistry, 284 (2019):28-44,
https://doi.org/10.1016/j.foodchem.2019.01.107 . .
72
34
73

Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System

Fotirić-Akšić, Milica; Dabić-Zagorac, Dragana; Sredojević, Milica; Milivojević, Jasminka; Gašić, Uroš M.; Meland, Mekjell; Natić, Maja

(MDPI, BASEL, 2019)

TY  - JOUR
AU  - Fotirić-Akšić, Milica
AU  - Dabić-Zagorac, Dragana
AU  - Sredojević, Milica
AU  - Milivojević, Jasminka
AU  - Gašić, Uroš M.
AU  - Meland, Mekjell
AU  - Natić, Maja
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4983
AB  - Chemical characterizations of leaves and fruits that were obtained from organically and integrally produced strawberries ('Favette', 'Alba', and 'Clery') and blueberries ('Bluecrop', 'Duke', and 'Nui') from western Serbia were undertaken in this study. Phenolic analysis was done while using ultra-high performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass analyzer, while total phenolic content (TPC), total anthocyanin content (TAC), and radical-scavenging activity (RSA) by spectrophotometry. In general, leaves and fruits from blueberry showed higher levels of TPC and TAC as compared to strawberry. These chemical traits were larger in organic grown fruits and larger in leaves than fruits. The most abundant phenolics in leaves and fruits of blueberry was 5-O-caffeoylquinic acid, followed by quercetin 3-O-galactoside, while catechin, quercetin, and kaempferol 3-O-glucosid were dominant in the leaves and fruits of strawberry. cis, trans-Abscisic acid was detected in all fruit samples, but not in leaves. Blueberries (both fruits and leaves) were separated from strawberries, but only organic blueberry fruits were distinguished from integrated fruits, according to principal component analysis. Quercetin, kaempferol, 5-O-caffeoylquinic acid, ferulic acid, caffeic acid, catechin, p-coumaric acid, and p-hydroxybenzoic acid were the most influential phenolic compounds for the separation. Much higher contents of TPC, RSA, TAC, quercetin 3-O-galactoside, and quercetin were found in fruits and TPC, RSA, catechin, p-hydroxybenzoicacid, p-coumaricacid, and ferulic acid in leaves in all three blueberry cultivars and the strawberry cultivar 'Clery'. These phenolic compounds are good sources of antioxidant compounds with potentially high beneficial effects on human health.
PB  - MDPI, BASEL
T2  - Molecules
T1  - Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System
IS  - 23
VL  - 24
DO  - 10.3390/molecules24234310
ER  - 
@article{
author = "Fotirić-Akšić, Milica and Dabić-Zagorac, Dragana and Sredojević, Milica and Milivojević, Jasminka and Gašić, Uroš M. and Meland, Mekjell and Natić, Maja",
year = "2019",
abstract = "Chemical characterizations of leaves and fruits that were obtained from organically and integrally produced strawberries ('Favette', 'Alba', and 'Clery') and blueberries ('Bluecrop', 'Duke', and 'Nui') from western Serbia were undertaken in this study. Phenolic analysis was done while using ultra-high performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass analyzer, while total phenolic content (TPC), total anthocyanin content (TAC), and radical-scavenging activity (RSA) by spectrophotometry. In general, leaves and fruits from blueberry showed higher levels of TPC and TAC as compared to strawberry. These chemical traits were larger in organic grown fruits and larger in leaves than fruits. The most abundant phenolics in leaves and fruits of blueberry was 5-O-caffeoylquinic acid, followed by quercetin 3-O-galactoside, while catechin, quercetin, and kaempferol 3-O-glucosid were dominant in the leaves and fruits of strawberry. cis, trans-Abscisic acid was detected in all fruit samples, but not in leaves. Blueberries (both fruits and leaves) were separated from strawberries, but only organic blueberry fruits were distinguished from integrated fruits, according to principal component analysis. Quercetin, kaempferol, 5-O-caffeoylquinic acid, ferulic acid, caffeic acid, catechin, p-coumaric acid, and p-hydroxybenzoic acid were the most influential phenolic compounds for the separation. Much higher contents of TPC, RSA, TAC, quercetin 3-O-galactoside, and quercetin were found in fruits and TPC, RSA, catechin, p-hydroxybenzoicacid, p-coumaricacid, and ferulic acid in leaves in all three blueberry cultivars and the strawberry cultivar 'Clery'. These phenolic compounds are good sources of antioxidant compounds with potentially high beneficial effects on human health.",
publisher = "MDPI, BASEL",
journal = "Molecules",
title = "Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System",
number = "23",
volume = "24",
doi = "10.3390/molecules24234310"
}
Fotirić-Akšić, M., Dabić-Zagorac, D., Sredojević, M., Milivojević, J., Gašić, U. M., Meland, M.,& Natić, M.. (2019). Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System. in Molecules
MDPI, BASEL., 24(23).
https://doi.org/10.3390/molecules24234310
Fotirić-Akšić M, Dabić-Zagorac D, Sredojević M, Milivojević J, Gašić UM, Meland M, Natić M. Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System. in Molecules. 2019;24(23).
doi:10.3390/molecules24234310 .
Fotirić-Akšić, Milica, Dabić-Zagorac, Dragana, Sredojević, Milica, Milivojević, Jasminka, Gašić, Uroš M., Meland, Mekjell, Natić, Maja, "Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System" in Molecules, 24, no. 23 (2019),
https://doi.org/10.3390/molecules24234310 . .
1
29
8

The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)

Cirković, Dušica; Matijašević, Saša; Deletić, Nebojša; Cirković, Bratislav; Gašić, Uroš M.; Sredojević, Milica; Jovanović, Zoran; Djurić, Vladan; Tešić, Živoslav

(MDPI, BASEL, 2019)

TY  - JOUR
AU  - Cirković, Dušica
AU  - Matijašević, Saša
AU  - Deletić, Nebojša
AU  - Cirković, Bratislav
AU  - Gašić, Uroš M.
AU  - Sredojević, Milica
AU  - Jovanović, Zoran
AU  - Djurić, Vladan
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4982
AB  - The influence of leaf area and various variants of manual defoliation on the phenolic profile of the Prokupac variety grape berry were investigated in the agroecological conditions of southern Serbia. The following four trial variants of manual defoliation were assessed: Early defoliation-variant I (flowering stage, 50% open flowers); early defoliation-variant II (grape size 3-5 mm); late defoliation-variant III (onset of grape ripening, veraison); and control (no defoliation). The first six leaves of each primary shoot were removed from all defoliated vines. The greatest assimilation area of primary and lateral shoots during the study was observed in the control, i.e., the trial variant with no defoliation. Defoliation significantly decreased the grape yield of the all three defoliated variants in regard to the control. The phenolic profile of the three variants and control was established by analyzing the grape seeds and skin. Based on the collected results for the Prokupac variety, significant differences between the trial variants were established regarding the content of phenols and total polyphenols, as well as radical scavenging activity. Defoliation variants showed a significant effect on the total phenols content of grape skin. In all defoliation variants, as well as in the control, high amounts of ellagic acid were measured. Resveratrol was identified only in grape skin samples of the control variant. The removal of leaves increased the concentration of phenolic compounds in variants where early defoliation was applied. The highest total anthocyanins content was found in 2015 in variant I, where leaves were removed during the full flowering stage.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)
IS  - 12
VL  - 9
DO  - 10.3390/agronomy9120822
ER  - 
@article{
author = "Cirković, Dušica and Matijašević, Saša and Deletić, Nebojša and Cirković, Bratislav and Gašić, Uroš M. and Sredojević, Milica and Jovanović, Zoran and Djurić, Vladan and Tešić, Živoslav",
year = "2019",
abstract = "The influence of leaf area and various variants of manual defoliation on the phenolic profile of the Prokupac variety grape berry were investigated in the agroecological conditions of southern Serbia. The following four trial variants of manual defoliation were assessed: Early defoliation-variant I (flowering stage, 50% open flowers); early defoliation-variant II (grape size 3-5 mm); late defoliation-variant III (onset of grape ripening, veraison); and control (no defoliation). The first six leaves of each primary shoot were removed from all defoliated vines. The greatest assimilation area of primary and lateral shoots during the study was observed in the control, i.e., the trial variant with no defoliation. Defoliation significantly decreased the grape yield of the all three defoliated variants in regard to the control. The phenolic profile of the three variants and control was established by analyzing the grape seeds and skin. Based on the collected results for the Prokupac variety, significant differences between the trial variants were established regarding the content of phenols and total polyphenols, as well as radical scavenging activity. Defoliation variants showed a significant effect on the total phenols content of grape skin. In all defoliation variants, as well as in the control, high amounts of ellagic acid were measured. Resveratrol was identified only in grape skin samples of the control variant. The removal of leaves increased the concentration of phenolic compounds in variants where early defoliation was applied. The highest total anthocyanins content was found in 2015 in variant I, where leaves were removed during the full flowering stage.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)",
number = "12",
volume = "9",
doi = "10.3390/agronomy9120822"
}
Cirković, D., Matijašević, S., Deletić, N., Cirković, B., Gašić, U. M., Sredojević, M., Jovanović, Z., Djurić, V.,& Tešić, Ž.. (2019). The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.). in Agronomy-Basel
MDPI, BASEL., 9(12).
https://doi.org/10.3390/agronomy9120822
Cirković D, Matijašević S, Deletić N, Cirković B, Gašić UM, Sredojević M, Jovanović Z, Djurić V, Tešić Ž. The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.). in Agronomy-Basel. 2019;9(12).
doi:10.3390/agronomy9120822 .
Cirković, Dušica, Matijašević, Saša, Deletić, Nebojša, Cirković, Bratislav, Gašić, Uroš M., Sredojević, Milica, Jovanović, Zoran, Djurić, Vladan, Tešić, Živoslav, "The Effect of Early and Late Defoliation on Phenolic Composition and Antioxidant Properties of Prokupac Variety Grape Berries (Vitis vinifera L.)" in Agronomy-Basel, 9, no. 12 (2019),
https://doi.org/10.3390/agronomy9120822 . .
1
6
2
5

Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen

Fotirić-Akšić, Milica; Gašić, Uroš M.; Dabić-Zagorac, Dragana; Sredojević, Milica; Tosti, Tomislav; Natić, Maja; Meland, Mekjell

(MDPI, BASEL, 2019)

TY  - JOUR
AU  - Fotirić-Akšić, Milica
AU  - Gašić, Uroš M.
AU  - Dabić-Zagorac, Dragana
AU  - Sredojević, Milica
AU  - Tosti, Tomislav
AU  - Natić, Maja
AU  - Meland, Mekjell
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4921
AB  - The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblainska' sour cherry clones and to assess the chemical fingerprint of this cultivar. Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system. Glucose was the most abundant sugar, followed by fructose and sucrose. Some samples had high level of stress sugars, especially trehalose. Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after. According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples. PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles. Large differences in chemical composition of studied 'Oblainska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population. Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.
PB  - MDPI, BASEL
T2  - Biomolecules
T1  - Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen
IS  - 9
VL  - 9
DO  - 10.3390/biom9090391
ER  - 
@article{
author = "Fotirić-Akšić, Milica and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Sredojević, Milica and Tosti, Tomislav and Natić, Maja and Meland, Mekjell",
year = "2019",
abstract = "The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblainska' sour cherry clones and to assess the chemical fingerprint of this cultivar. Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system. Glucose was the most abundant sugar, followed by fructose and sucrose. Some samples had high level of stress sugars, especially trehalose. Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after. According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples. PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles. Large differences in chemical composition of studied 'Oblainska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population. Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.",
publisher = "MDPI, BASEL",
journal = "Biomolecules",
title = "Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen",
number = "9",
volume = "9",
doi = "10.3390/biom9090391"
}
Fotirić-Akšić, M., Gašić, U. M., Dabić-Zagorac, D., Sredojević, M., Tosti, T., Natić, M.,& Meland, M.. (2019). Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen. in Biomolecules
MDPI, BASEL., 9(9).
https://doi.org/10.3390/biom9090391
Fotirić-Akšić M, Gašić UM, Dabić-Zagorac D, Sredojević M, Tosti T, Natić M, Meland M. Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen. in Biomolecules. 2019;9(9).
doi:10.3390/biom9090391 .
Fotirić-Akšić, Milica, Gašić, Uroš M., Dabić-Zagorac, Dragana, Sredojević, Milica, Tosti, Tomislav, Natić, Maja, Meland, Mekjell, "Chemical Fingerprint of 'Oblainska' Sour Cherry (Prunus cerasus L.) Pollen" in Biomolecules, 9, no. 9 (2019),
https://doi.org/10.3390/biom9090391 . .
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2
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Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones

Fotirić-Akšić, Milica; Guffa, B.; Gašić, Uroš M.; Dabić-Zagorac, Dragana; Natić, Maja; Meland, Mekjell

(International Society for Horticultural Science, 2019)

TY  - CONF
AU  - Fotirić-Akšić, Milica
AU  - Guffa, B.
AU  - Gašić, Uroš M.
AU  - Dabić-Zagorac, Dragana
AU  - Natić, Maja
AU  - Meland, Mekjell
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5135
AB  - The presence of pollinators in orchards is crucial to obtain high fruit set and yields of fruits. Despite the fact that sour cherry cultivars are mainly autogamous, insect visits are still of great importance for their propagation. In order to attract and reward pollinators, flowers have to provide adequate nourishment to them. Besides nectar, bees gather pollen, which are a prerequisite for normal colony growth and development of their broods. 'Oblačinska' sour cherry (Prunus cerasus L.), an autochthonous cultivar, is the most highly planted cultivar in Serbian commercial orchards. Since the cultivar is actually a mixture of different clones, variability in numerous traits and, particularly, its yields has been reported. Since phenolic compounds are considered to be fundamental pollen chemicals, the aim of this study was to determine the phenolic compounds profile in pollen collected from 15 'Oblačinska' sour cherry clones with varying productivity levels. Solid phase extraction (SPE), combined with ultra-high-performance liquid chromatography coupled with a diode array detector and a triple quadruple mass spectrometer (UHPLC DAD-MS/MS), was used to analyse the polyphenolic profile of pollen. Among 23 components quantified, rutin was the most abundant phenolic compound. It ranged from 98.49 (clone V/P) to 358.83 mg kg-1 (clone III/9) and was observed to contribute, on average, 56% of the total phenolic compounds in pollen as quantified in different 'Oblačinska' sour cherry clones. In addition to this compound, clones contained significant amounts of chlorogenic acid (12.92%), astragalin (8.19%), and hyperoside (5.59%) as well. Cluster analysis grouped pollen clones in four different clusters, which showed that clones III/9, IV/8, and V/P had unique phenolic profiles. Despite the significant differences among the studied clones, the contents of chlorogenic acid, rutin, naringin, hyperoside, astralgin, and phlorizin were distinguishable between the clusters.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones
EP  - 394
SP  - 387
VL  - 1235
DO  - 10.17660/ActaHortic.2019.1235.53
ER  - 
@conference{
author = "Fotirić-Akšić, Milica and Guffa, B. and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Natić, Maja and Meland, Mekjell",
year = "2019",
abstract = "The presence of pollinators in orchards is crucial to obtain high fruit set and yields of fruits. Despite the fact that sour cherry cultivars are mainly autogamous, insect visits are still of great importance for their propagation. In order to attract and reward pollinators, flowers have to provide adequate nourishment to them. Besides nectar, bees gather pollen, which are a prerequisite for normal colony growth and development of their broods. 'Oblačinska' sour cherry (Prunus cerasus L.), an autochthonous cultivar, is the most highly planted cultivar in Serbian commercial orchards. Since the cultivar is actually a mixture of different clones, variability in numerous traits and, particularly, its yields has been reported. Since phenolic compounds are considered to be fundamental pollen chemicals, the aim of this study was to determine the phenolic compounds profile in pollen collected from 15 'Oblačinska' sour cherry clones with varying productivity levels. Solid phase extraction (SPE), combined with ultra-high-performance liquid chromatography coupled with a diode array detector and a triple quadruple mass spectrometer (UHPLC DAD-MS/MS), was used to analyse the polyphenolic profile of pollen. Among 23 components quantified, rutin was the most abundant phenolic compound. It ranged from 98.49 (clone V/P) to 358.83 mg kg-1 (clone III/9) and was observed to contribute, on average, 56% of the total phenolic compounds in pollen as quantified in different 'Oblačinska' sour cherry clones. In addition to this compound, clones contained significant amounts of chlorogenic acid (12.92%), astragalin (8.19%), and hyperoside (5.59%) as well. Cluster analysis grouped pollen clones in four different clusters, which showed that clones III/9, IV/8, and V/P had unique phenolic profiles. Despite the significant differences among the studied clones, the contents of chlorogenic acid, rutin, naringin, hyperoside, astralgin, and phlorizin were distinguishable between the clusters.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones",
pages = "394-387",
volume = "1235",
doi = "10.17660/ActaHortic.2019.1235.53"
}
Fotirić-Akšić, M., Guffa, B., Gašić, U. M., Dabić-Zagorac, D., Natić, M.,& Meland, M.. (2019). Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones. in Acta Horticulturae
International Society for Horticultural Science., 1235, 387-394.
https://doi.org/10.17660/ActaHortic.2019.1235.53
Fotirić-Akšić M, Guffa B, Gašić UM, Dabić-Zagorac D, Natić M, Meland M. Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones. in Acta Horticulturae. 2019;1235:387-394.
doi:10.17660/ActaHortic.2019.1235.53 .
Fotirić-Akšić, Milica, Guffa, B., Gašić, Uroš M., Dabić-Zagorac, Dragana, Natić, Maja, Meland, Mekjell, "Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones" in Acta Horticulturae, 1235 (2019):387-394,
https://doi.org/10.17660/ActaHortic.2019.1235.53 . .