TY  - JOUR
AU  - Núñez, Yolanda
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - García-Casco, Juan M.
AU  - Čandek-Potokar, Marjeta
AU  - Benítez, Rita
AU  - Radojković, Dragan
AU  - Lukić, Miloš
AU  - Gogić, Marija
AU  - Muñoz, María
AU  - Fontanesi, Luca
AU  - Óvilo, Cristina
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5827
AB  - This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
PB  - MDPI AG
T2  - Animals
T1  - Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
EP  - 24
IS  - 3
SP  - 1
VL  - 11
DO  - 10.3390/ani11030844
ER  - 

@article{
author = "Núñez, Yolanda and Radović, Čedomir and Savić, Radomir and García-Casco, Juan M. and Čandek-Potokar, Marjeta and Benítez, Rita and Radojković, Dragan and Lukić, Miloš and Gogić, Marija and Muñoz, María and Fontanesi, Luca and Óvilo, Cristina",
year = "2021",
abstract = "This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.",
publisher = "MDPI AG",
journal = "Animals",
title = "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs",
pages = "24-1",
number = "3",
volume = "11",
doi = "10.3390/ani11030844"
}

Núñez, Y., Radović, Č., Savić, R., García-Casco, J. M., Čandek-Potokar, M., Benítez, R., Radojković, D., Lukić, M., Gogić, M., Muñoz, M., Fontanesi, L.,& Óvilo, C.. (2021). Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals
MDPI AG., 11(3), 1-24.
https://doi.org/10.3390/ani11030844

Núñez Y, Radović Č, Savić R, García-Casco JM, Čandek-Potokar M, Benítez R, Radojković D, Lukić M, Gogić M, Muñoz M, Fontanesi L, Óvilo C. Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals. 2021;11(3):1-24.
doi:10.3390/ani11030844 .

Núñez, Yolanda, Radović, Čedomir, Savić, Radomir, García-Casco, Juan M., Čandek-Potokar, Marjeta, Benítez, Rita, Radojković, Dragan, Lukić, Miloš, Gogić, Marija, Muñoz, María, Fontanesi, Luca, Óvilo, Cristina, "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs" in Animals, 11, no. 3 (2021):1-24,
https://doi.org/10.3390/ani11030844 . .