TY  - JOUR
AU  - Mirković, Nemanja
AU  - Radulović, Zorica
AU  - Uzelac, Gordana
AU  - Lozo, Jelena
AU  - Obradović, Dragojlo
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3845
AB  - Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain
EP  - 242
IS  - 2
SP  - 237
VL  - 53
DO  - 10.17113/ftb.53.02.15.3846
ER  - 

@article{
author = "Mirković, Nemanja and Radulović, Zorica and Uzelac, Gordana and Lozo, Jelena and Obradović, Dragojlo and Topisirović, Ljubiša and Kojić, Milan",
year = "2015",
abstract = "Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain",
pages = "242-237",
number = "2",
volume = "53",
doi = "10.17113/ftb.53.02.15.3846"
}

Mirković, N., Radulović, Z., Uzelac, G., Lozo, J., Obradović, D., Topisirović, L.,& Kojić, M.. (2015). Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology
University of Zagreb., 53(2), 237-242.
https://doi.org/10.17113/ftb.53.02.15.3846

Mirković N, Radulović Z, Uzelac G, Lozo J, Obradović D, Topisirović L, Kojić M. Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology. 2015;53(2):237-242.
doi:10.17113/ftb.53.02.15.3846 .

Mirković, Nemanja, Radulović, Zorica, Uzelac, Gordana, Lozo, Jelena, Obradović, Dragojlo, Topisirović, Ljubiša, Kojić, Milan, "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain" in Food Technology and Biotechnology, 53, no. 2 (2015):237-242,
https://doi.org/10.17113/ftb.53.02.15.3846 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Tolinacki, M.
AU  - Nikolić, M.
AU  - Veljović, Katarina
AU  - Jovanović, Snežana
AU  - Maćej, Ognjen
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3161
AB  - The aim of this study was the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheeses for the selection of potential starter cultures. Soft white Vlasina cheeses were manufactured at a household on the Stara Planina Mountain without starter cultures using traditional techniques. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheeses, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in cheeses has been characterized by phenotype-based assays and presumptively identified using repetitive element palindromic-polymerase chain reaction (rep-PCR) with the primer (GTG)5. Results have been confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15 d-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis subsp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exopolysaccharides while BGVL2-29 and BGVL2-63 that belonged to the species Ln. pseudomesenteroides utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in a starter culture. This study contributed to the understanding of the role of autochthonous LAB on quality of artisanal cheeses and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Artisanal vlasina raw goat's milk cheeses: Evaluation and selection of autochthonous lactic acid bacteria as starter cultures
IS  - 4
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3161
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Tolinacki, M. and Nikolić, M. and Veljović, Katarina and Jovanović, Snežana and Maćej, Ognjen and Topisirović, Ljubiša",
year = "2013",
abstract = "The aim of this study was the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheeses for the selection of potential starter cultures. Soft white Vlasina cheeses were manufactured at a household on the Stara Planina Mountain without starter cultures using traditional techniques. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheeses, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in cheeses has been characterized by phenotype-based assays and presumptively identified using repetitive element palindromic-polymerase chain reaction (rep-PCR) with the primer (GTG)5. Results have been confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15 d-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis subsp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exopolysaccharides while BGVL2-29 and BGVL2-63 that belonged to the species Ln. pseudomesenteroides utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in a starter culture. This study contributed to the understanding of the role of autochthonous LAB on quality of artisanal cheeses and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Artisanal vlasina raw goat's milk cheeses: Evaluation and selection of autochthonous lactic acid bacteria as starter cultures",
number = "4",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3161"
}

Terzić-Vidojević, A., Tolinacki, M., Nikolić, M., Veljović, K., Jovanović, S., Maćej, O.,& Topisirović, L.. (2013). Artisanal vlasina raw goat's milk cheeses: Evaluation and selection of autochthonous lactic acid bacteria as starter cultures. in Food Technology and Biotechnology
University of Zagreb., 51(4).
https://hdl.handle.net/21.15107/rcub_agrospace_3161

Terzić-Vidojević A, Tolinacki M, Nikolić M, Veljović K, Jovanović S, Maćej O, Topisirović L. Artisanal vlasina raw goat's milk cheeses: Evaluation and selection of autochthonous lactic acid bacteria as starter cultures. in Food Technology and Biotechnology. 2013;51(4).
https://hdl.handle.net/21.15107/rcub_agrospace_3161 .

Terzić-Vidojević, Amarela, Tolinacki, M., Nikolić, M., Veljović, Katarina, Jovanović, Snežana, Maćej, Ognjen, Topisirović, Ljubiša, "Artisanal vlasina raw goat's milk cheeses: Evaluation and selection of autochthonous lactic acid bacteria as starter cultures" in Food Technology and Biotechnology, 51, no. 4 (2013),
https://hdl.handle.net/21.15107/rcub_agrospace_3161 .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Tolinacki, Maja
AU  - Nikolić, Milica
AU  - Veljović, Katarina
AU  - Jovanović, Snežana
AU  - Maćej, Ognjen
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3131
AB  - The aim of this study is the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheese for the selection of potential starter cultures. Soft white Vlasina cheese was manufactured at a household on the Stara Planina Mountain using traditional techniques without starter cultures. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheese, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in the cheese samples was characterized by phenotype-based assays and presumptively identified using repetitive element palindromic polymerase chain reaction (rep-PCR) with the primer (GTG)(5). Results were confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15-day-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis ssp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exo-polysaccharides, while BGVL2-29 and BGVL2-63, which belonged to the species Ln. pseudomesenteroides, utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in the starter culture. This study contributed to the understanding of the role of autochthonous LAB in the quality of artisanal cheese and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures
EP  - 563
IS  - 4
SP  - 554
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3131
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Tolinacki, Maja and Nikolić, Milica and Veljović, Katarina and Jovanović, Snežana and Maćej, Ognjen and Topisirović, Ljubiša",
year = "2013",
abstract = "The aim of this study is the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheese for the selection of potential starter cultures. Soft white Vlasina cheese was manufactured at a household on the Stara Planina Mountain using traditional techniques without starter cultures. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheese, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in the cheese samples was characterized by phenotype-based assays and presumptively identified using repetitive element palindromic polymerase chain reaction (rep-PCR) with the primer (GTG)(5). Results were confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15-day-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis ssp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exo-polysaccharides, while BGVL2-29 and BGVL2-63, which belonged to the species Ln. pseudomesenteroides, utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in the starter culture. This study contributed to the understanding of the role of autochthonous LAB in the quality of artisanal cheese and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures",
pages = "563-554",
number = "4",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3131"
}

Terzić-Vidojević, A., Tolinacki, M., Nikolić, M., Veljović, K., Jovanović, S., Maćej, O.,& Topisirović, L.. (2013). Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 51(4), 554-563.
https://hdl.handle.net/21.15107/rcub_agrospace_3131

Terzić-Vidojević A, Tolinacki M, Nikolić M, Veljović K, Jovanović S, Maćej O, Topisirović L. Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures. in Food Technology and Biotechnology. 2013;51(4):554-563.
https://hdl.handle.net/21.15107/rcub_agrospace_3131 .

Terzić-Vidojević, Amarela, Tolinacki, Maja, Nikolić, Milica, Veljović, Katarina, Jovanović, Snežana, Maćej, Ognjen, Topisirović, Ljubiša, "Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures" in Food Technology and Biotechnology, 51, no. 4 (2013):554-563,
https://hdl.handle.net/21.15107/rcub_agrospace_3131 .

TY  - JOUR
AU  - Ostojić, Mihailo S.
AU  - Topisirović, Ljubiša
AU  - Relić, Renata
AU  - Jež, Goran M.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2264
AB  - Autochthonous cheese manufacturing within the area of Serbia has very long tradition. These cheeses are produced without using any known starter culture. Therefore, studying of all aspects of such cheese manufacturing is very important. This approach will assure continuation of traditional way of manufacturing cheeses at the places of their origin. Moreover, research of this kind will open possibility for introduction of autochthonous cheese production at either semi-industrial or industrial level, or their presentation on different markets as well. Golija cheese belongs to the group of white soft cheeses. It is produced from nonpasteurized raw cow milk immediately after milking. Normally it is kept on the mountain for one to two months for ripening. Cheese is traditionally cut into slices and packed in distribution packaging. This production is mostly carried out by small manufacturers, what results in uneven quality and ungraded value of cheese. However, due to production in reserved region of origin, demands for Protected Denomination of Origin (PDO) were satisfied. Aim of this work was to investigate autochthonous cheese manufacturing, chemical composition and microbiological quality of cheese produced on mountain Golija. Investigation of the fluctuation of lactic acid bacteria (LAB) population in Golija cheese was made by analysis of the presence of LAB in the milk of which the cheese was made, as well as in the cheese during ripening. The composition of cheese microflora was recorded in cheeses after 1, 10, 20, 30, 45 and 60 days of ripening.
AB  - Autohtona proizvodnja sireva na području Srbije ima veoma dugu tradiciju. Ovi sirevi se proizvode bez korišćenja starter kulture. Iz tog razloga, proučavanje svih aspekata proizvodnje takvih sireva je veoma važno. Ovakav pristup će obezbediti nastavak tradicionalnog načina proizvodnje sireva na područjima odakle i potiču. Osim toga, ovakva istraživanja stvoriće mogućnost predstavljanja proizvodnje autohtonih sireva, bilo na poluindustrijskom ili industrijskom nivou, kao i njihovo prezentovanje na različitim tržištima. Golijski sir pripada grupi mekih sireva. Proizvodi se od nepasterizovanog (sirovog) kravljeg mleka, koje se u tu svrhu koristi odmah posle muže. Uobičajeno je da sir ostaje u planinskom domaćinstvu jedan ili dva meseca dok traje period zrenja. Sir se tradicionalno seče u kriške i pakuje u odgovarajuću ambalažu. Ovu proizvodnju uglavnom vrše mali proizvođači, što ima za posledicu neujednačen kvalitet i nestandardizovanu vrednost sira. Međutim, proizvodnjom u zaštićenim regionima, zadovoljeni su zahtevi za oznaku geografskog porekla. Cilj ovog rada bio je istraživanje autohtone proizvodnje, hemijski sastav i mikrobiološki kvalitet sira proizvedenog na planini Golija. Ispitivanje promena u populaciji bakterija mlečne kiseline (BMK) u siru sa Golije izvršeno je analizom na prisustvo BMK u mleku za proizvodnju sira, kao i u siru za vreme zrenja. Sastav mikroflore sira praćen je posle 1, 10, 20, 30, 45 i 60 dana zrenja sira.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd
T2  - Prehrambena industrija - mleko i mlečni proizvodi
T1  - Autochthonous technology of Golija cheese
T1  - Autohtona tehnologija proizvodnje Golijskog sira
EP  - 51
IS  - 1-2
SP  - 46
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2264
ER  - 

@article{
author = "Ostojić, Mihailo S. and Topisirović, Ljubiša and Relić, Renata and Jež, Goran M.",
year = "2010",
abstract = "Autochthonous cheese manufacturing within the area of Serbia has very long tradition. These cheeses are produced without using any known starter culture. Therefore, studying of all aspects of such cheese manufacturing is very important. This approach will assure continuation of traditional way of manufacturing cheeses at the places of their origin. Moreover, research of this kind will open possibility for introduction of autochthonous cheese production at either semi-industrial or industrial level, or their presentation on different markets as well. Golija cheese belongs to the group of white soft cheeses. It is produced from nonpasteurized raw cow milk immediately after milking. Normally it is kept on the mountain for one to two months for ripening. Cheese is traditionally cut into slices and packed in distribution packaging. This production is mostly carried out by small manufacturers, what results in uneven quality and ungraded value of cheese. However, due to production in reserved region of origin, demands for Protected Denomination of Origin (PDO) were satisfied. Aim of this work was to investigate autochthonous cheese manufacturing, chemical composition and microbiological quality of cheese produced on mountain Golija. Investigation of the fluctuation of lactic acid bacteria (LAB) population in Golija cheese was made by analysis of the presence of LAB in the milk of which the cheese was made, as well as in the cheese during ripening. The composition of cheese microflora was recorded in cheeses after 1, 10, 20, 30, 45 and 60 days of ripening., Autohtona proizvodnja sireva na području Srbije ima veoma dugu tradiciju. Ovi sirevi se proizvode bez korišćenja starter kulture. Iz tog razloga, proučavanje svih aspekata proizvodnje takvih sireva je veoma važno. Ovakav pristup će obezbediti nastavak tradicionalnog načina proizvodnje sireva na područjima odakle i potiču. Osim toga, ovakva istraživanja stvoriće mogućnost predstavljanja proizvodnje autohtonih sireva, bilo na poluindustrijskom ili industrijskom nivou, kao i njihovo prezentovanje na različitim tržištima. Golijski sir pripada grupi mekih sireva. Proizvodi se od nepasterizovanog (sirovog) kravljeg mleka, koje se u tu svrhu koristi odmah posle muže. Uobičajeno je da sir ostaje u planinskom domaćinstvu jedan ili dva meseca dok traje period zrenja. Sir se tradicionalno seče u kriške i pakuje u odgovarajuću ambalažu. Ovu proizvodnju uglavnom vrše mali proizvođači, što ima za posledicu neujednačen kvalitet i nestandardizovanu vrednost sira. Međutim, proizvodnjom u zaštićenim regionima, zadovoljeni su zahtevi za oznaku geografskog porekla. Cilj ovog rada bio je istraživanje autohtone proizvodnje, hemijski sastav i mikrobiološki kvalitet sira proizvedenog na planini Golija. Ispitivanje promena u populaciji bakterija mlečne kiseline (BMK) u siru sa Golije izvršeno je analizom na prisustvo BMK u mleku za proizvodnju sira, kao i u siru za vreme zrenja. Sastav mikroflore sira praćen je posle 1, 10, 20, 30, 45 i 60 dana zrenja sira.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd",
journal = "Prehrambena industrija - mleko i mlečni proizvodi",
title = "Autochthonous technology of Golija cheese, Autohtona tehnologija proizvodnje Golijskog sira",
pages = "51-46",
number = "1-2",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2264"
}

Ostojić, M. S., Topisirović, L., Relić, R.,& Jež, G. M.. (2010). Autochthonous technology of Golija cheese. in Prehrambena industrija - mleko i mlečni proizvodi
Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd., 21(1-2), 46-51.
https://hdl.handle.net/21.15107/rcub_agrospace_2264

Ostojić MS, Topisirović L, Relić R, Jež GM. Autochthonous technology of Golija cheese. in Prehrambena industrija - mleko i mlečni proizvodi. 2010;21(1-2):46-51.
https://hdl.handle.net/21.15107/rcub_agrospace_2264 .

Ostojić, Mihailo S., Topisirović, Ljubiša, Relić, Renata, Jež, Goran M., "Autochthonous technology of Golija cheese" in Prehrambena industrija - mleko i mlečni proizvodi, 21, no. 1-2 (2010):46-51,
https://hdl.handle.net/21.15107/rcub_agrospace_2264 .