TY  - JOUR
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Weldon, William A.
AU  - Stockwell, Virginia O.
AU  - Pothier, Joël F.
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6461
AB  - Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.
T2  - Frontiers in Plant Science
T2  - Frontiers in Plant Science
T1  - Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6461
ER  - 

@article{
author = "Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Weldon, William A. and Stockwell, Virginia O. and Pothier, Joël F.",
year = "2023",
abstract = "Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.",
journal = "Frontiers in Plant Science, Frontiers in Plant Science",
title = "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6461"
}

Kałużna, M., Prokić, A., Obradović, A., Weldon, W. A., Stockwell, V. O.,& Pothier, J. F.. (2023). Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science, 14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461

Kałużna M, Prokić A, Obradović A, Weldon WA, Stockwell VO, Pothier JF. Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science. 2023;14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Weldon, William A., Stockwell, Virginia O., Pothier, Joël F., "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics" in Frontiers in Plant Science, 14 (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

TY  - JOUR
AU  - Pothier, Joël F
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Rezzonico, Fabio
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6069
AB  - Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.
PB  - NLM (Medline)
T2  - Phytopathology
T1  - Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut
EP  - 960
IS  - 4
SP  - 956
VL  - 112
DO  - 10.1094/PHYTO-08-21-0356-A
ER  - 

@article{
author = "Pothier, Joël F and Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Rezzonico, Fabio",
year = "2022",
abstract = "Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.",
publisher = "NLM (Medline)",
journal = "Phytopathology",
title = "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut",
pages = "960-956",
number = "4",
volume = "112",
doi = "10.1094/PHYTO-08-21-0356-A"
}

Pothier, J. F., Kałużna, M., Prokić, A., Obradović, A.,& Rezzonico, F.. (2022). Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology
NLM (Medline)., 112(4), 956-960.
https://doi.org/10.1094/PHYTO-08-21-0356-A

Pothier JF, Kałużna M, Prokić A, Obradović A, Rezzonico F. Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology. 2022;112(4):956-960.
doi:10.1094/PHYTO-08-21-0356-A .

Pothier, Joël F, Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Rezzonico, Fabio, "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut" in Phytopathology, 112, no. 4 (2022):956-960,
https://doi.org/10.1094/PHYTO-08-21-0356-A . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pavlović, Z.
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5379
AB  - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
EP  - 691
IS  - 3
SP  - 685
VL  - 157
DO  - 10.1007/s10658-020-02019-4
ER  - 

@article{
author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa",
year = "2020",
abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia",
pages = "691-685",
number = "3",
volume = "157",
doi = "10.1007/s10658-020-02019-4"
}

Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 157(3), 685-691.
https://doi.org/10.1007/s10658-020-02019-4

Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691.
doi:10.1007/s10658-020-02019-4 .

Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691,
https://doi.org/10.1007/s10658-020-02019-4 . .

TY  - JOUR
AU  - Popović, T.
AU  - Menković, Jelena
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5325
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro
EP  - 561
IS  - 2
SP  - 560
VL  - 104
DO  - 10.1094/PDIS-07-19-1422-PDN
ER  - 

@article{
author = "Popović, T. and Menković, Jelena and Prokić, Andjelka and Obradović, Aleksa",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro",
pages = "561-560",
number = "2",
volume = "104",
doi = "10.1094/PDIS-07-19-1422-PDN"
}

Popović, T., Menković, J., Prokić, A.,& Obradović, A.. (2020). First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(2), 560-561.
https://doi.org/10.1094/PDIS-07-19-1422-PDN

Popović T, Menković J, Prokić A, Obradović A. First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease. 2020;104(2):560-561.
doi:10.1094/PDIS-07-19-1422-PDN .

Popović, T., Menković, Jelena, Prokić, Andjelka, Obradović, Aleksa, "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro" in Plant Disease, 104, no. 2 (2020):560-561,
https://doi.org/10.1094/PDIS-07-19-1422-PDN . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4912
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia
EP  - 2667
IS  - 10
SP  - 2667
VL  - 103
DO  - 10.1094/PDIS-12-18-2213-PDN
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Gašić, K. and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2019",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia",
pages = "2667-2667",
number = "10",
volume = "103",
doi = "10.1094/PDIS-12-18-2213-PDN"
}

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(10), 2667-2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667-2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Andjelka, Gašić, K., Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667-2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5009
AB  - Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material
EP  - 1052
IS  - 3
SP  - 1039
VL  - 51
DO  - 10.2298/GENSR1903039I
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Gašić, Katarina and Prokić, Andjelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2019",
abstract = "Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material",
pages = "1052-1039",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903039I"
}

Ivanović, M., Kuzmanović, N., Gašić, K., Prokić, A., Zlatković, N.,& Obradović, A.. (2019). Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika
Društvo genetičara Srbije, Beograd., 51(3), 1039-1052.
https://doi.org/10.2298/GENSR1903039I

Ivanović M, Kuzmanović N, Gašić K, Prokić A, Zlatković N, Obradović A. Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika. 2019;51(3):1039-1052.
doi:10.2298/GENSR1903039I .

Ivanović, Milan, Kuzmanović, Nemanja, Gašić, Katarina, Prokić, Andjelka, Zlatković, Nevena, Obradović, Aleksa, "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material" in Genetika, 51, no. 3 (2019):1039-1052,
https://doi.org/10.2298/GENSR1903039I . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5221
AB  - Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species.
AB  - Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of cucurbits
T1  - Bakterioze tikava
EP  - 445
IS  - 6
SP  - 431
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5221
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species., Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of cucurbits, Bakterioze tikava",
pages = "445-431",
number = "6",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5221"
}

Zlatković, N., Gašić, K., Prokić, A.,& Obradović, A.. (2019). Bacterial diseases of cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(6), 431-445.
https://hdl.handle.net/21.15107/rcub_agrospace_5221

Zlatković N, Gašić K, Prokić A, Obradović A. Bacterial diseases of cucurbits. in Biljni lekar. 2019;47(6):431-445.
https://hdl.handle.net/21.15107/rcub_agrospace_5221 .

Zlatković, Nevena, Gašić, Katarina, Prokić, Andjelka, Obradović, Aleksa, "Bacterial diseases of cucurbits" in Biljni lekar, 47, no. 6 (2019):431-445,
https://hdl.handle.net/21.15107/rcub_agrospace_5221 .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5017
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier Sci Ltd, Oxford., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Andjelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - CONF
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4646
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4646
ER  - 

@conference{
author = "Gašić, K. and Kuzmanović, Nemanja and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4646"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

Gašić, K., Kuzmanović, Nemanja, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133,
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

TY  - CONF
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4645
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4645
ER  - 

@conference{
author = "Prokić, Andjelka and Ivanović, Milan and Gašić, K. and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4645"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

Prokić, Andjelka, Ivanović, Milan, Gašić, K., Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24,
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4662
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Dreo, Tanja
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4838
AB  - Bacterial blight of grapevine, caused by phytopathogenic bacterium Xylophilus ampelinus, is economically important disease that can significantly affect grapevine production, leading to the overall yield reduction and reduced vitality of infected grapevines. In regions where disease was recorded, losses in production, especially in susceptible varieties, can reach up to 80% of total yield. The bacterium infects only grapevine. In infected plants, the pathogen is located in the vascular tissues (xylem) from where it spreads further, causing a systemic infection of the host plant. During vegetation it is transmitted from plant to plant to short distance and the main source of inoculum for long distance dissemination are infected cuttings used either as rootstocks or grafting material. There are no completely resistant varieties or effective methods for controlling the disease. In Serbia X. ampelinus has a quarantine status. Therefore, it is of utmost importance to prevent introduction of the pathogen by inspecting the health of reproductive plant material, especially of those originating from countries where the pathogen is already present.
AB  - Bakteriozna plamenjača vinove loze, koju prouzrokuje fitopatogena bakterija Xylophilus ampelinus, je ekonomski značajno oboljenje koje može ugroziti proizvodnju vinove loze umanjujući ukupan prinos i smanjujući dugovečnost zaraženih biljaka. U regionima gde je zabeleženo prisustvo bolesti gubici u proizvodnji, naročito kod osetljivih sorti, mogu dostići i do 80% ukupnog prinosa. Krug domaćina ove bakterije ograničen je samo na vinovu lozu. U zaraženim biljkama patogen se nalazi u sudovnom sistemu (ksilemu) odakle se dalje širi prouzrokujući sistemičnu infekciju biljke domaćina. Tokom vegetacije prenosi se u neposrednoj okolini izvora zaraze sa biljke na biljku, a na veću udaljenost zaraženim sadnim materijalom koji predstavlja primarni izvor inokuluma. Ne postoje potpuno otporne sorte ni dovoljno efikasne metode zaštite od ove bolesti. U Srbiji X. ampelinus ima karantinski status. Stoga je od najvećeg značaja sprečavanje unošenja patogena kontrolom zdravstvene ispravnosti biljnog materijala za reprodukciju, posebno iz zemalja gde je utvrđeno prisustvo patogena.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial blight of grapevine: Xylophilus ampelinus
T1  - Bakteriozna plamenjača vinove loze - Xylophilus ampelinus
EP  - 680
IS  - 6
SP  - 674
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4838
ER  - 

@article{
author = "Prokić, Andjelka and Dreo, Tanja and Obradović, Aleksa",
year = "2018",
abstract = "Bacterial blight of grapevine, caused by phytopathogenic bacterium Xylophilus ampelinus, is economically important disease that can significantly affect grapevine production, leading to the overall yield reduction and reduced vitality of infected grapevines. In regions where disease was recorded, losses in production, especially in susceptible varieties, can reach up to 80% of total yield. The bacterium infects only grapevine. In infected plants, the pathogen is located in the vascular tissues (xylem) from where it spreads further, causing a systemic infection of the host plant. During vegetation it is transmitted from plant to plant to short distance and the main source of inoculum for long distance dissemination are infected cuttings used either as rootstocks or grafting material. There are no completely resistant varieties or effective methods for controlling the disease. In Serbia X. ampelinus has a quarantine status. Therefore, it is of utmost importance to prevent introduction of the pathogen by inspecting the health of reproductive plant material, especially of those originating from countries where the pathogen is already present., Bakteriozna plamenjača vinove loze, koju prouzrokuje fitopatogena bakterija Xylophilus ampelinus, je ekonomski značajno oboljenje koje može ugroziti proizvodnju vinove loze umanjujući ukupan prinos i smanjujući dugovečnost zaraženih biljaka. U regionima gde je zabeleženo prisustvo bolesti gubici u proizvodnji, naročito kod osetljivih sorti, mogu dostići i do 80% ukupnog prinosa. Krug domaćina ove bakterije ograničen je samo na vinovu lozu. U zaraženim biljkama patogen se nalazi u sudovnom sistemu (ksilemu) odakle se dalje širi prouzrokujući sistemičnu infekciju biljke domaćina. Tokom vegetacije prenosi se u neposrednoj okolini izvora zaraze sa biljke na biljku, a na veću udaljenost zaraženim sadnim materijalom koji predstavlja primarni izvor inokuluma. Ne postoje potpuno otporne sorte ni dovoljno efikasne metode zaštite od ove bolesti. U Srbiji X. ampelinus ima karantinski status. Stoga je od najvećeg značaja sprečavanje unošenja patogena kontrolom zdravstvene ispravnosti biljnog materijala za reprodukciju, posebno iz zemalja gde je utvrđeno prisustvo patogena.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial blight of grapevine: Xylophilus ampelinus, Bakteriozna plamenjača vinove loze - Xylophilus ampelinus",
pages = "680-674",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4838"
}

Prokić, A., Dreo, T.,& Obradović, A.. (2018). Bacterial blight of grapevine: Xylophilus ampelinus. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 674-680.
https://hdl.handle.net/21.15107/rcub_agrospace_4838

Prokić A, Dreo T, Obradović A. Bacterial blight of grapevine: Xylophilus ampelinus. in Biljni lekar. 2018;46(6):674-680.
https://hdl.handle.net/21.15107/rcub_agrospace_4838 .

Prokić, Andjelka, Dreo, Tanja, Obradović, Aleksa, "Bacterial blight of grapevine: Xylophilus ampelinus" in Biljni lekar, 46, no. 6 (2018):674-680,
https://hdl.handle.net/21.15107/rcub_agrospace_4838 .

TY  - JOUR
AU  - Jerinić-Prodanović, Dušanka
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Pavlović, Žaklina
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4551
AB  - Carrot psyllid Bactericera trigonica belongs to the family Triozidae, superfamily Psylloidea, order Hemiptera. Together with onion psyllid (B. tremblayi) and potato psyllid (B. nigricornis), it is a member of the complex Bactericera nigricornis Foerster with morphologically and bioecologically very similar species. It was originally described by Hodkinson (1981) as Trioza trigonica based on the analysis of adults collected from the carrots in Portugal, Italy, Cyprus, Turkey, Egypt and Iran. Later it has been transferred to the genus Bactericera together with the two other species from this complex. Adults and larvae B. trigonica, feed on carrot leaf and other Apiaceae. If they appears in large number, they can lead to chlorosis and leaf yellowing, which causes a yield reduction. Besides direct damage, its vector role of bacteria “Candidatus Liberibacter solanacearum” has been determined, which leads to root proliferation and weakening of plants from Apiaceae family due to chlorosis. Ca. Liberibacter solanacearum has been known in North Africa and New Zealand for a long time on plants from Solanacae family, potato in the first place, while in Europe it has been determined on carrot and cellery for the first time, with B. trigonica as its vector. The presence, biology and harmfulness of B. trigonica in the region of Serbia were studyed in the period from 2014 to 2017. It was found in 2014 for the first time and since then, its presence has been determined in all researched localities, during the whole vegetative period of carrot. Besides carrot, B. trigonica has been registered on celery, parsley and wild carrot as well. It has a several generations per year, overwintering as adults.
AB  - Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi) i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericera nigricornis Foerster, u morfološkom i bioekološkom smislu veoma sličnih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebačena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u povećanoj brojnosti mogu dovesti do hloroze i žućenja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvrđena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hlorotičnog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvrđena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine proučavano je prisustvo, biologija i štetnost B. trigonica na području Srbije. Prvi put je registrovana 2014. godine i od tada je utvrđeno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima veći broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia
T1  - Rasprostranjenost, biologija i štetnost mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) u Srbiji
EP  - 384
IS  - 4
SP  - 375
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4551
ER  - 

@article{
author = "Jerinić-Prodanović, Dušanka and Obradović, Aleksa and Ivanović, Milan and Prokić, Andjelka and Zlatković, Nevena and Pavlović, Žaklina",
year = "2017",
abstract = "Carrot psyllid Bactericera trigonica belongs to the family Triozidae, superfamily Psylloidea, order Hemiptera. Together with onion psyllid (B. tremblayi) and potato psyllid (B. nigricornis), it is a member of the complex Bactericera nigricornis Foerster with morphologically and bioecologically very similar species. It was originally described by Hodkinson (1981) as Trioza trigonica based on the analysis of adults collected from the carrots in Portugal, Italy, Cyprus, Turkey, Egypt and Iran. Later it has been transferred to the genus Bactericera together with the two other species from this complex. Adults and larvae B. trigonica, feed on carrot leaf and other Apiaceae. If they appears in large number, they can lead to chlorosis and leaf yellowing, which causes a yield reduction. Besides direct damage, its vector role of bacteria “Candidatus Liberibacter solanacearum” has been determined, which leads to root proliferation and weakening of plants from Apiaceae family due to chlorosis. Ca. Liberibacter solanacearum has been known in North Africa and New Zealand for a long time on plants from Solanacae family, potato in the first place, while in Europe it has been determined on carrot and cellery for the first time, with B. trigonica as its vector. The presence, biology and harmfulness of B. trigonica in the region of Serbia were studyed in the period from 2014 to 2017. It was found in 2014 for the first time and since then, its presence has been determined in all researched localities, during the whole vegetative period of carrot. Besides carrot, B. trigonica has been registered on celery, parsley and wild carrot as well. It has a several generations per year, overwintering as adults., Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi) i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericera nigricornis Foerster, u morfološkom i bioekološkom smislu veoma sličnih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebačena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u povećanoj brojnosti mogu dovesti do hloroze i žućenja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvrđena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hlorotičnog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvrđena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine proučavano je prisustvo, biologija i štetnost B. trigonica na području Srbije. Prvi put je registrovana 2014. godine i od tada je utvrđeno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima veći broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia, Rasprostranjenost, biologija i štetnost mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) u Srbiji",
pages = "384-375",
number = "4",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4551"
}

Jerinić-Prodanović, D., Obradović, A., Ivanović, M., Prokić, A., Zlatković, N.,& Pavlović, Ž.. (2017). Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(4), 375-384.
https://hdl.handle.net/21.15107/rcub_agrospace_4551

Jerinić-Prodanović D, Obradović A, Ivanović M, Prokić A, Zlatković N, Pavlović Ž. Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar. 2017;45(4):375-384.
https://hdl.handle.net/21.15107/rcub_agrospace_4551 .

Jerinić-Prodanović, Dušanka, Obradović, Aleksa, Ivanović, Milan, Prokić, Andjelka, Zlatković, Nevena, Pavlović, Žaklina, "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia" in Biljni lekar, 45, no. 4 (2017):375-384,
https://hdl.handle.net/21.15107/rcub_agrospace_4551 .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4556
AB  - Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide.
AB  - Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of Cucurbits
T1  - Bakteriozna oboljenja biljaka familije Cucurbitaceae
EP  - 400
IS  - 4
SP  - 390
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4556
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Kuzmanović, Nemanja and Gašić, Katarina and Ivanović, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide., Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of Cucurbits, Bakteriozna oboljenja biljaka familije Cucurbitaceae",
pages = "400-390",
number = "4",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4556"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Ivanović, M.,& Obradović, A.. (2017). Bacterial diseases of Cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(4), 390-400.
https://hdl.handle.net/21.15107/rcub_agrospace_4556

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Ivanović M, Obradović A. Bacterial diseases of Cucurbits. in Biljni lekar. 2017;45(4):390-400.
https://hdl.handle.net/21.15107/rcub_agrospace_4556 .

Zlatković, Nevena, Prokić, Andjelka, Kuzmanović, Nemanja, Gašić, Katarina, Ivanović, Milan, Obradović, Aleksa, "Bacterial diseases of Cucurbits" in Biljni lekar, 45, no. 4 (2017):390-400,
https://hdl.handle.net/21.15107/rcub_agrospace_4556 .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jerinić-Prodanović, Dušanka
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Pavlović, Žaklina
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4301
AB  - Candidatus Liberibacter solanacearumis a gram-negative bacterium restricted to plant phloem, associated with economically important diseases such as potato zebra chip and yellow decline and with vegetative disorders in carrots and celery. Over the past decade, the zebra chip disease resulted in major economic loses in the potato production of U.S.A., Mexico, Central America and New Zealand. Outbreaks of yellow decline and vegetative disorders in carrot and celery crops have been reported recently in geographically distant areas and the countries of Europe (Finland, Sweden, Norway, Spain, France and Germany). The infected plants showed symptoms including yellow, bronze or red leaf discoloration, reduced size of main root and lateral root proliferation. Given the importance of carrot production in Serbia, the goal of this review paper is to raise awareness about the disease occurrence and distribution, discuss the main characteristics of this relatively unknown pathogen, its detection and identification, epidemiology, symptomatology and disease management, before this emerging plant pathogenic organism reaches our country.
AB  - Fastidiozna bakterija 'Candidatus Liberibacter solanacearum' poznata je kao prouzrokovač destruktivnog oboljenja 'zebrasti čips', a od nedavno i kao prouzrokovač bolesti hlorotičnog slabljenja biljaka iz familije Apiaceae. Kao patogen krompira 'Ca. L. solanacearum' poznat je već deceniju unazad u proizvodnim regionima Amerike i Novog Zelanda. Međutim, na evropskom kontinentu, prisustvo ove bakterije prvi put je potvrđeno na zaraženim biljkama mrkve i celera, što je ukazalo na širi krug domaćina ovog patogena. Areal rasprostranjenja u Evropi obuhvata Finsku, Švedsku, Norvešku, Španiju, Francusku i Nemačku. Kao posledica infekcije dolazi do promena u boji listova, kržljavosti izdanaka, dok najznačajnije štete nastaju usled proliferacije korena. S obzirom na značaj proizvodnje mrkve u našoj zemlji, cilj ovog preglednog rada je da se ukaže na pojavu i rasprostranjenost navedenog destruktivnog oboljenja, karakteristike patogena, simptomatologiju, načine prenošenja, metode detekcije i identifikacije neophodne za pouzdanu i pravovremenu dijagnozu oboljenja, kao i mere zaštite pre nego što ovaj patogen dospe u našu zemlju.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family
T1  - 'Candidatus Liberibacter solanacearum' - novi patogen biljaka iz familije Apiaceae
EP  - 191
IS  - 2
SP  - 180
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4301
ER  - 

@article{
author = "Obradović, Aleksa and Jerinić-Prodanović, Dušanka and Ivanović, Milan and Prokić, Andjelka and Kuzmanović, Nemanja and Zlatković, Nevena and Pavlović, Žaklina",
year = "2016",
abstract = "Candidatus Liberibacter solanacearumis a gram-negative bacterium restricted to plant phloem, associated with economically important diseases such as potato zebra chip and yellow decline and with vegetative disorders in carrots and celery. Over the past decade, the zebra chip disease resulted in major economic loses in the potato production of U.S.A., Mexico, Central America and New Zealand. Outbreaks of yellow decline and vegetative disorders in carrot and celery crops have been reported recently in geographically distant areas and the countries of Europe (Finland, Sweden, Norway, Spain, France and Germany). The infected plants showed symptoms including yellow, bronze or red leaf discoloration, reduced size of main root and lateral root proliferation. Given the importance of carrot production in Serbia, the goal of this review paper is to raise awareness about the disease occurrence and distribution, discuss the main characteristics of this relatively unknown pathogen, its detection and identification, epidemiology, symptomatology and disease management, before this emerging plant pathogenic organism reaches our country., Fastidiozna bakterija 'Candidatus Liberibacter solanacearum' poznata je kao prouzrokovač destruktivnog oboljenja 'zebrasti čips', a od nedavno i kao prouzrokovač bolesti hlorotičnog slabljenja biljaka iz familije Apiaceae. Kao patogen krompira 'Ca. L. solanacearum' poznat je već deceniju unazad u proizvodnim regionima Amerike i Novog Zelanda. Međutim, na evropskom kontinentu, prisustvo ove bakterije prvi put je potvrđeno na zaraženim biljkama mrkve i celera, što je ukazalo na širi krug domaćina ovog patogena. Areal rasprostranjenja u Evropi obuhvata Finsku, Švedsku, Norvešku, Španiju, Francusku i Nemačku. Kao posledica infekcije dolazi do promena u boji listova, kržljavosti izdanaka, dok najznačajnije štete nastaju usled proliferacije korena. S obzirom na značaj proizvodnje mrkve u našoj zemlji, cilj ovog preglednog rada je da se ukaže na pojavu i rasprostranjenost navedenog destruktivnog oboljenja, karakteristike patogena, simptomatologiju, načine prenošenja, metode detekcije i identifikacije neophodne za pouzdanu i pravovremenu dijagnozu oboljenja, kao i mere zaštite pre nego što ovaj patogen dospe u našu zemlju.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family, 'Candidatus Liberibacter solanacearum' - novi patogen biljaka iz familije Apiaceae",
pages = "191-180",
number = "2",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4301"
}

Obradović, A., Jerinić-Prodanović, D., Ivanović, M., Prokić, A., Kuzmanović, N., Zlatković, N.,& Pavlović, Ž.. (2016). 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(2), 180-191.
https://hdl.handle.net/21.15107/rcub_agrospace_4301

Obradović A, Jerinić-Prodanović D, Ivanović M, Prokić A, Kuzmanović N, Zlatković N, Pavlović Ž. 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family. in Biljni lekar. 2016;44(2):180-191.
https://hdl.handle.net/21.15107/rcub_agrospace_4301 .

Obradović, Aleksa, Jerinić-Prodanović, Dušanka, Ivanović, Milan, Prokić, Andjelka, Kuzmanović, Nemanja, Zlatković, Nevena, Pavlović, Žaklina, "'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family" in Biljni lekar, 44, no. 2 (2016):180-191,
https://hdl.handle.net/21.15107/rcub_agrospace_4301 .

TY  - CONF
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4098
AB  - Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia
EP  - 719
SP  - 715
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.122
ER  - 

@conference{
author = "Ivanović, Milan and Gašić, K. and Prokić, Andjelka and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2016",
abstract = "Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia",
pages = "719-715",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.122"
}

Ivanović, M., Gašić, K., Prokić, A., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2016). Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 715-719.
https://doi.org/10.17660/ActaHortic.2016.1139.122

Ivanović M, Gašić K, Prokić A, Kuzmanović N, Zlatković N, Obradović A. Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing. 2016;1139:715-719.
doi:10.17660/ActaHortic.2016.1139.122 .

Ivanović, Milan, Gašić, K., Prokić, Andjelka, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia" in III Balkan Symposium on Fruit Growing, 1139 (2016):715-719,
https://doi.org/10.17660/ActaHortic.2016.1139.122 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4044
AB  - Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall
EP  - 319
IS  - 2
SP  - 311
VL  - 98
DO  - 10.4454/JPP.V98I2.028
ER  - 

@article{
author = "Kuzmanović, Nemanja and Biondi, E. and Ivanović, Milan and Prokić, Andjelka and Zlatković, Nevena and Bertaccini, Assunta and Obradović, Aleksa",
year = "2016",
abstract = "Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall",
pages = "319-311",
number = "2",
volume = "98",
doi = "10.4454/JPP.V98I2.028"
}

Kuzmanović, N., Biondi, E., Ivanović, M., Prokić, A., Zlatković, N., Bertaccini, A.,& Obradović, A.. (2016). Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology
Springer, New York., 98(2), 311-319.
https://doi.org/10.4454/JPP.V98I2.028

Kuzmanović N, Biondi E, Ivanović M, Prokić A, Zlatković N, Bertaccini A, Obradović A. Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology. 2016;98(2):311-319.
doi:10.4454/JPP.V98I2.028 .

Kuzmanović, Nemanja, Biondi, E., Ivanović, Milan, Prokić, Andjelka, Zlatković, Nevena, Bertaccini, Assunta, Obradović, Aleksa, "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall" in Journal of Plant Pathology, 98, no. 2 (2016):311-319,
https://doi.org/10.4454/JPP.V98I2.028 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Jerinić-Prodanović, Dušanka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3889
AB  - Candidatus Liberibacter solanacearum (CaLsol) is a phloem-limited pathogen and it was found associated with the 'zebra chip' potato disease. This pathogen causes signifi cant economic losses by reducing the yield and quality of potato crops. It is widespread in commercial potato fi elds in USA, Mexico, Central America ,New Zealand. CaLsol can also affect other solanaceous crops, including tomato, pepper, eggplant, tamarillo. Recently, it was detected in several Northern European countries in carrot and celery crops. Chips made from zebra chip-infected tubers show dark stripes that become more visible upon frying, and are therefore commercially unacceptable. Bearing in mind the importance of potato production in our country, it is necessary to raise the awareness about the disease occurrence and distribution.
AB  - Među najznačajnijim štetnim organizmima koji ugrožavaju proizvodnju krompira od nedavno se ubraja fastidiozna bakterija 'Candidatus Liberibacter solanacearum' (CaLsol). Ovaj patogen odgovoran je za pojavu novog oboljenja krompira pod nazivom 'zebrasti čips' (ZČ), rasprostranjenog u proizvodnim područjima Amerike i Novog Zelanda. Iako je glavni domaćin patogena krompir, ozbiljne štete zabeležene su i na biljkama paprike, paradajza i drugim gajenim vrstama iz familije Solanaceae. U Evropi isti patogen nedavno je otkriven na obolelim biljkama mrkve i celera. Na krompiru, kao posledica infekcije dolazi do smanjenja prinosa zaraženih biljaka, a najznačajnije štete nastaju usled pojave simptoma na krtolama i gubitka njihove tržišne vrednosti. S obzirom na ekonomski značaj proizvodnje krompira u našoj zemlji, kao i na mogućnost unošenja bakterije putem vegetativnog sadnog materijala, postoji opravdana zabrinutost od širenja ovog patogena na teritoriji naše zemlje.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - 'Candidatus Liberibacter solanacearum': causal agent of potato 'zebra chip' disease
T1  - 'Candidatus Liberibacter solanacearum' - prouzrokovač 'zebrastog čipsa' krompira
EP  - 550
IS  - 6
SP  - 541
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3889
ER  - 

@article{
author = "Obradović, Aleksa and Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Jerinić-Prodanović, Dušanka",
year = "2015",
abstract = "Candidatus Liberibacter solanacearum (CaLsol) is a phloem-limited pathogen and it was found associated with the 'zebra chip' potato disease. This pathogen causes signifi cant economic losses by reducing the yield and quality of potato crops. It is widespread in commercial potato fi elds in USA, Mexico, Central America ,New Zealand. CaLsol can also affect other solanaceous crops, including tomato, pepper, eggplant, tamarillo. Recently, it was detected in several Northern European countries in carrot and celery crops. Chips made from zebra chip-infected tubers show dark stripes that become more visible upon frying, and are therefore commercially unacceptable. Bearing in mind the importance of potato production in our country, it is necessary to raise the awareness about the disease occurrence and distribution., Među najznačajnijim štetnim organizmima koji ugrožavaju proizvodnju krompira od nedavno se ubraja fastidiozna bakterija 'Candidatus Liberibacter solanacearum' (CaLsol). Ovaj patogen odgovoran je za pojavu novog oboljenja krompira pod nazivom 'zebrasti čips' (ZČ), rasprostranjenog u proizvodnim područjima Amerike i Novog Zelanda. Iako je glavni domaćin patogena krompir, ozbiljne štete zabeležene su i na biljkama paprike, paradajza i drugim gajenim vrstama iz familije Solanaceae. U Evropi isti patogen nedavno je otkriven na obolelim biljkama mrkve i celera. Na krompiru, kao posledica infekcije dolazi do smanjenja prinosa zaraženih biljaka, a najznačajnije štete nastaju usled pojave simptoma na krtolama i gubitka njihove tržišne vrednosti. S obzirom na ekonomski značaj proizvodnje krompira u našoj zemlji, kao i na mogućnost unošenja bakterije putem vegetativnog sadnog materijala, postoji opravdana zabrinutost od širenja ovog patogena na teritoriji naše zemlje.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "'Candidatus Liberibacter solanacearum': causal agent of potato 'zebra chip' disease, 'Candidatus Liberibacter solanacearum' - prouzrokovač 'zebrastog čipsa' krompira",
pages = "550-541",
number = "6",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3889"
}

Obradović, A., Prokić, A., Zlatković, N., Kuzmanović, N.,& Jerinić-Prodanović, D.. (2015). 'Candidatus Liberibacter solanacearum': causal agent of potato 'zebra chip' disease. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(6), 541-550.
https://hdl.handle.net/21.15107/rcub_agrospace_3889

Obradović A, Prokić A, Zlatković N, Kuzmanović N, Jerinić-Prodanović D. 'Candidatus Liberibacter solanacearum': causal agent of potato 'zebra chip' disease. in Biljni lekar. 2015;43(6):541-550.
https://hdl.handle.net/21.15107/rcub_agrospace_3889 .

Obradović, Aleksa, Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Jerinić-Prodanović, Dušanka, "'Candidatus Liberibacter solanacearum': causal agent of potato 'zebra chip' disease" in Biljni lekar, 43, no. 6 (2015):541-550,
https://hdl.handle.net/21.15107/rcub_agrospace_3889 .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3880
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3880
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3880"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

Zlatković, Nevena, Prokić, Andjelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271,
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3819
AB  - During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia
EP  - 713
IS  - 4
SP  - 701
VL  - 142
DO  - 10.1007/s10658-015-0645-4
ER  - 

@article{
author = "Kuzmanović, Nemanja and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia",
pages = "713-701",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0645-4"
}

Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 701-713.
https://doi.org/10.1007/s10658-015-0645-4

Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology. 2015;142(4):701-713.
doi:10.1007/s10658-015-0645-4 .

Kuzmanović, Nemanja, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia" in European Journal of Plant Pathology, 142, no. 4 (2015):701-713,
https://doi.org/10.1007/s10658-015-0645-4 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3730
AB  - Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology Resource Announcements
T1  - Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330
IS  - 2
VL  - 3
DO  - 10.1128/genomeA.00331-15
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology Resource Announcements",
title = "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330",
number = "2",
volume = "3",
doi = "10.1128/genomeA.00331-15"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements
Amer Soc Microbiology, Washington., 3(2).
https://doi.org/10.1128/genomeA.00331-15

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements. 2015;3(2).
doi:10.1128/genomeA.00331-15 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330" in Microbiology Resource Announcements, 3, no. 2 (2015),
https://doi.org/10.1128/genomeA.00331-15 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Jones, Jeffrey B.
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
AB  - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
PB  - Elsevier Gmbh, Munich
T2  - Systematic and Applied Microbiology
T1  - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP  - 378
IS  - 6
SP  - 373
VL  - 38
DO  - 10.1016/j.syapm.2015.06.001
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa",
year = "2015",
abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).",
publisher = "Elsevier Gmbh, Munich",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier Gmbh, Munich., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .

TY  - THES
AU  - Prokić, Andjelka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/29
AB  - Bacterial blight of hazelnut, caused by Xanthomonas arboricola pv. corylina (Xac), is the most important bacterial disease of this nut crop worldwide. In our country, after the disease was initially described in the 1950s, there were no further data about the research of this pathogen. During the growing season of 2008-2011, symptoms of leaf spots, bud and twig necrosis and dieback, suggesting bacterial infection, were observed on hazelnut plants in several localities in Serbia. In order to study etiology of disease, symptomatic samples of diseased hazelnut plants were collected from different hazelnut-growing regions in the country. Isolations from the diseased tissue resulted in Gram and oxidase negative, catalase-positive, aerobic yellow bacterial colonies. Total of 47 representative strains were selected for further study. Strains isolated from hazelnut in Poland and various reference strains of Xac originating from international collections were also included in research. Based on their morphological, pathogenic, biochemical, physiological, serological and genetic characteristics tested strains were identified as Xanthomonas arboricola pv. corylina. Bacterial colonies formed on NA medium were small and glistening and on glucose amended GYCA medium large, mucous and domed. All strains hydrolyzed starch, gelatin and esculin, used glucose, maltose, sucrose as a carbon source, but not sorbitol, metabolized quinates, developed at 35 °C in the presence of 2% NaCl. The strains induced hypersensitive reaction of tobacco, tomato leaves and bean pods. Pathogenic nature of the strains was confirmed in pathogenicity test on host plants by reproduction of symptoms similar to natural infection. Based on carbon source utilization, metabolic diversity of investigated strains was confirmed. Strain's metabolic profiles were specific for Xanthomonas genus. Serological methods (ELISA i IF test) indicated similarity in serological characteristics among the studied strains, as well as the control Xac strain...
AB  - Bakteriozna plamenjača leske, čiji je prouzrokovač fitopatogena bakterija Xanthomonas arboricola pv. corylina (Xac), najznačajnije je bakteriozno oboljenje ove jezgraste voćke u svetu. U našoj zemlji bolest je prvi put uočena 50-ih godina prošlog veka, nakon čega nije bilo daljih proučavanja ovog patogena. U periodu 2008 - 2011. godine, u nekoliko rejona gajenja leske u Srbiji uočena je pojava simptoma pegavosti lišća, nekroze i uvelosti pupoljaka i letorasta, koji su ukazivali na moguću bakterioznu prirodu oboljenja. Simptomatični uzorci obolelih biljaka prikupljeni su sa različitih lokaliteta u našoj zemlji sa ciljem utvrđivanja etiologije oboljenja. Iz zaraženog biljnog tkiva izolovani su Gram i oksidaza negativni, katalazapozitivni aerobni sojevi bakterija žute boje kolnija na HA podlozi. Za dalji rad odabrano je ukupno 47 reprezentativnih sojeva, koji su proučeni primenom standardnih i savremenih metoda. U istraživanja su uključeni i sojevi poreklom iz Poljske, kao i referentni sojevi Xac poreklom iz međunarodnih kolekcija. Detaljno su proučene morfološke, patogene, biohemijsko-fiziološke, serološke i genetičke odlike sojeva, na osnovu kojih su svi proučavani sojevi identifikovani kao Xanthomonas arboricola pv. corylina. Na podlozi od hranljivog agara (HA) formirane su sitne i sjajne kolonije, dok su one na podlozi obogaćenoj glukozom (GYCA) bile krupne, sluzaste i ispupčene. Svi proučavani sojevi hidrolizuju skrob, želatin i eskulin, koriste glukozu, maltozu, saharozu kao izvor ugljenika, ali ne i sorbitol, metabolišu kvinate, razvijaju se pri 35°C i u prisustvu 2% NaCl. Sojevi su prouzrokovali hipersenzitivnu reakciju na listovima duvana i paradajza i mahunama boranije. Patogenost sojeva potvrđena je testom patogenosti na domaćinu i reprodukcijom simptoma prirodne infekcije. Na osnovu Biolog testa utvrđena je raznovrsnost proučavanih sojeva čiji su metabolički profili bili specifični za rod Xanthomonas...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Xanthomonas arboricola pv. corylina - identification of the pathogen and population biodiversity
T1  - Xanthomonas arboricola pv. corylina - identifikacija patogena i biodiverzitet populacije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5703
ER  - 

@phdthesis{
author = "Prokić, Andjelka",
year = "2014",
abstract = "Bacterial blight of hazelnut, caused by Xanthomonas arboricola pv. corylina (Xac), is the most important bacterial disease of this nut crop worldwide. In our country, after the disease was initially described in the 1950s, there were no further data about the research of this pathogen. During the growing season of 2008-2011, symptoms of leaf spots, bud and twig necrosis and dieback, suggesting bacterial infection, were observed on hazelnut plants in several localities in Serbia. In order to study etiology of disease, symptomatic samples of diseased hazelnut plants were collected from different hazelnut-growing regions in the country. Isolations from the diseased tissue resulted in Gram and oxidase negative, catalase-positive, aerobic yellow bacterial colonies. Total of 47 representative strains were selected for further study. Strains isolated from hazelnut in Poland and various reference strains of Xac originating from international collections were also included in research. Based on their morphological, pathogenic, biochemical, physiological, serological and genetic characteristics tested strains were identified as Xanthomonas arboricola pv. corylina. Bacterial colonies formed on NA medium were small and glistening and on glucose amended GYCA medium large, mucous and domed. All strains hydrolyzed starch, gelatin and esculin, used glucose, maltose, sucrose as a carbon source, but not sorbitol, metabolized quinates, developed at 35 °C in the presence of 2% NaCl. The strains induced hypersensitive reaction of tobacco, tomato leaves and bean pods. Pathogenic nature of the strains was confirmed in pathogenicity test on host plants by reproduction of symptoms similar to natural infection. Based on carbon source utilization, metabolic diversity of investigated strains was confirmed. Strain's metabolic profiles were specific for Xanthomonas genus. Serological methods (ELISA i IF test) indicated similarity in serological characteristics among the studied strains, as well as the control Xac strain..., Bakteriozna plamenjača leske, čiji je prouzrokovač fitopatogena bakterija Xanthomonas arboricola pv. corylina (Xac), najznačajnije je bakteriozno oboljenje ove jezgraste voćke u svetu. U našoj zemlji bolest je prvi put uočena 50-ih godina prošlog veka, nakon čega nije bilo daljih proučavanja ovog patogena. U periodu 2008 - 2011. godine, u nekoliko rejona gajenja leske u Srbiji uočena je pojava simptoma pegavosti lišća, nekroze i uvelosti pupoljaka i letorasta, koji su ukazivali na moguću bakterioznu prirodu oboljenja. Simptomatični uzorci obolelih biljaka prikupljeni su sa različitih lokaliteta u našoj zemlji sa ciljem utvrđivanja etiologije oboljenja. Iz zaraženog biljnog tkiva izolovani su Gram i oksidaza negativni, katalazapozitivni aerobni sojevi bakterija žute boje kolnija na HA podlozi. Za dalji rad odabrano je ukupno 47 reprezentativnih sojeva, koji su proučeni primenom standardnih i savremenih metoda. U istraživanja su uključeni i sojevi poreklom iz Poljske, kao i referentni sojevi Xac poreklom iz međunarodnih kolekcija. Detaljno su proučene morfološke, patogene, biohemijsko-fiziološke, serološke i genetičke odlike sojeva, na osnovu kojih su svi proučavani sojevi identifikovani kao Xanthomonas arboricola pv. corylina. Na podlozi od hranljivog agara (HA) formirane su sitne i sjajne kolonije, dok su one na podlozi obogaćenoj glukozom (GYCA) bile krupne, sluzaste i ispupčene. Svi proučavani sojevi hidrolizuju skrob, želatin i eskulin, koriste glukozu, maltozu, saharozu kao izvor ugljenika, ali ne i sorbitol, metabolišu kvinate, razvijaju se pri 35°C i u prisustvu 2% NaCl. Sojevi su prouzrokovali hipersenzitivnu reakciju na listovima duvana i paradajza i mahunama boranije. Patogenost sojeva potvrđena je testom patogenosti na domaćinu i reprodukcijom simptoma prirodne infekcije. Na osnovu Biolog testa utvrđena je raznovrsnost proučavanih sojeva čiji su metabolički profili bili specifični za rod Xanthomonas...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Xanthomonas arboricola pv. corylina - identification of the pathogen and population biodiversity, Xanthomonas arboricola pv. corylina - identifikacija patogena i biodiverzitet populacije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5703"
}

Prokić, A.. (2014). Xanthomonas arboricola pv. corylina - identification of the pathogen and population biodiversity. 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_5703

Prokić A. Xanthomonas arboricola pv. corylina - identification of the pathogen and population biodiversity. 2014;.
https://hdl.handle.net/21.15107/rcub_nardus_5703 .

Prokić, Andjelka, "Xanthomonas arboricola pv. corylina - identification of the pathogen and population biodiversity" (2014),
https://hdl.handle.net/21.15107/rcub_nardus_5703 .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Blagojević, N.
AU  - Obradović, Aleksa
AU  - Gašić, K.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3522
AB  - In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR
EP  - 84
SP  - 81
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.10
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Blagojević, N. and Obradović, Aleksa and Gašić, K.",
year = "2014",
abstract = "In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR",
pages = "84-81",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.10"
}

Ivanović, M., Kuzmanović, N., Prokić, A., Blagojević, N., Obradović, A.,& Gašić, K.. (2014). Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae
International Society for Horticultural Science., 1056, 81-84.
https://doi.org/10.17660/ActaHortic.2014.1056.10

Ivanović M, Kuzmanović N, Prokić A, Blagojević N, Obradović A, Gašić K. Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae. 2014;1056:81-84.
doi:10.17660/ActaHortic.2014.1056.10 .

Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Blagojević, N., Obradović, Aleksa, Gašić, K., "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR" in Acta Horticulturae, 1056 (2014):81-84,
https://doi.org/10.17660/ActaHortic.2014.1056.10 . .

TY  - JOUR
AU  - Gašić, K.
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3510
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 

@article{
author = "Gašić, K. and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}

Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae
International Society for Horticultural Science., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17

Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .

Gašić, K., Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .