TY  - JOUR
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Weldon, William A.
AU  - Stockwell, Virginia O.
AU  - Pothier, Joël F.
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6461
AB  - Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.
T2  - Frontiers in Plant Science
T2  - Frontiers in Plant Science
T1  - Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6461
ER  - 

@article{
author = "Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Weldon, William A. and Stockwell, Virginia O. and Pothier, Joël F.",
year = "2023",
abstract = "Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.",
journal = "Frontiers in Plant Science, Frontiers in Plant Science",
title = "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6461"
}

Kałużna, M., Prokić, A., Obradović, A., Weldon, W. A., Stockwell, V. O.,& Pothier, J. F.. (2023). Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science, 14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461

Kałużna M, Prokić A, Obradović A, Weldon WA, Stockwell VO, Pothier JF. Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science. 2023;14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Weldon, William A., Stockwell, Virginia O., Pothier, Joël F., "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics" in Frontiers in Plant Science, 14 (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

TY  - JOUR
AU  - Ismail, Emadeldeen
AU  - Blom, Jochen
AU  - Bultreys, Alain
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - van Doorn, Joop
AU  - Bergsma-Vlami, Maria
AU  - Maes, Martine
AU  - Willems, Anne
AU  - Duffy, Brion
AU  - Stockwell, Virginia O.
AU  - Smits, Theo H.M.
AU  - Pulawska, Joanna
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3470
AB  - Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.
PB  - Springer, New York
T2  - Archives of Microbiology
T1  - A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids
EP  - 899
IS  - 12
SP  - 891
VL  - 196
DO  - 10.1007/s00203-014-1028-5
ER  - 

@article{
author = "Ismail, Emadeldeen and Blom, Jochen and Bultreys, Alain and Ivanović, Milan and Obradović, Aleksa and van Doorn, Joop and Bergsma-Vlami, Maria and Maes, Martine and Willems, Anne and Duffy, Brion and Stockwell, Virginia O. and Smits, Theo H.M. and Pulawska, Joanna",
year = "2014",
abstract = "Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.",
publisher = "Springer, New York",
journal = "Archives of Microbiology",
title = "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids",
pages = "899-891",
number = "12",
volume = "196",
doi = "10.1007/s00203-014-1028-5"
}

Ismail, E., Blom, J., Bultreys, A., Ivanović, M., Obradović, A., van Doorn, J., Bergsma-Vlami, M., Maes, M., Willems, A., Duffy, B., Stockwell, V. O., Smits, T. H.M.,& Pulawska, J.. (2014). A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology
Springer, New York., 196(12), 891-899.
https://doi.org/10.1007/s00203-014-1028-5

Ismail E, Blom J, Bultreys A, Ivanović M, Obradović A, van Doorn J, Bergsma-Vlami M, Maes M, Willems A, Duffy B, Stockwell VO, Smits TH, Pulawska J. A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology. 2014;196(12):891-899.
doi:10.1007/s00203-014-1028-5 .

Ismail, Emadeldeen, Blom, Jochen, Bultreys, Alain, Ivanović, Milan, Obradović, Aleksa, van Doorn, Joop, Bergsma-Vlami, Maria, Maes, Martine, Willems, Anne, Duffy, Brion, Stockwell, Virginia O., Smits, Theo H.M., Pulawska, Joanna, "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids" in Archives of Microbiology, 196, no. 12 (2014):891-899,
https://doi.org/10.1007/s00203-014-1028-5 . .