TY  - JOUR
AU  - Pothier, Joël F
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Rezzonico, Fabio
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6069
AB  - Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.
PB  - NLM (Medline)
T2  - Phytopathology
T1  - Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut
EP  - 960
IS  - 4
SP  - 956
VL  - 112
DO  - 10.1094/PHYTO-08-21-0356-A
ER  - 

@article{
author = "Pothier, Joël F and Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Rezzonico, Fabio",
year = "2022",
abstract = "Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.",
publisher = "NLM (Medline)",
journal = "Phytopathology",
title = "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut",
pages = "960-956",
number = "4",
volume = "112",
doi = "10.1094/PHYTO-08-21-0356-A"
}

Pothier, J. F., Kałużna, M., Prokić, A., Obradović, A.,& Rezzonico, F.. (2022). Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology
NLM (Medline)., 112(4), 956-960.
https://doi.org/10.1094/PHYTO-08-21-0356-A

Pothier JF, Kałużna M, Prokić A, Obradović A, Rezzonico F. Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology. 2022;112(4):956-960.
doi:10.1094/PHYTO-08-21-0356-A .

Pothier, Joël F, Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Rezzonico, Fabio, "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut" in Phytopathology, 112, no. 4 (2022):956-960,
https://doi.org/10.1094/PHYTO-08-21-0356-A . .

TY  - JOUR
AU  - Kurz, Mirjam
AU  - Carnal, Simon
AU  - Dafny-Yelin, Mery
AU  - Mairesse, Orly
AU  - Gottsberger, Richard A.
AU  - Ivanović, Milan
AU  - Grahovac, Mila
AU  - Lagonenko, Alexander L.
AU  - Drenova, Nataliya
AU  - Zharmukhamedova, Galiya
AU  - Doolotkeldieva, Tinatin
AU  - Smits, Theo H. M.
AU  - Rezzonico, Fabio
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5916
AB  - Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.
PB  - BioMed Central Ltd
T2  - Phytopathology Research
T1  - Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer
IS  - 1
SP  - 18
VL  - 3
DO  - 10.1186/s42483-021-00096-9
ER  - 

@article{
author = "Kurz, Mirjam and Carnal, Simon and Dafny-Yelin, Mery and Mairesse, Orly and Gottsberger, Richard A. and Ivanović, Milan and Grahovac, Mila and Lagonenko, Alexander L. and Drenova, Nataliya and Zharmukhamedova, Galiya and Doolotkeldieva, Tinatin and Smits, Theo H. M. and Rezzonico, Fabio",
year = "2021",
abstract = "Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.",
publisher = "BioMed Central Ltd",
journal = "Phytopathology Research",
title = "Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer",
number = "1",
pages = "18",
volume = "3",
doi = "10.1186/s42483-021-00096-9"
}

Kurz, M., Carnal, S., Dafny-Yelin, M., Mairesse, O., Gottsberger, R. A., Ivanović, M., Grahovac, M., Lagonenko, A. L., Drenova, N., Zharmukhamedova, G., Doolotkeldieva, T., Smits, T. H. M.,& Rezzonico, F.. (2021). Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. in Phytopathology Research
BioMed Central Ltd., 3(1), 18.
https://doi.org/10.1186/s42483-021-00096-9

Kurz M, Carnal S, Dafny-Yelin M, Mairesse O, Gottsberger RA, Ivanović M, Grahovac M, Lagonenko AL, Drenova N, Zharmukhamedova G, Doolotkeldieva T, Smits THM, Rezzonico F. Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. in Phytopathology Research. 2021;3(1):18.
doi:10.1186/s42483-021-00096-9 .

Kurz, Mirjam, Carnal, Simon, Dafny-Yelin, Mery, Mairesse, Orly, Gottsberger, Richard A., Ivanović, Milan, Grahovac, Mila, Lagonenko, Alexander L., Drenova, Nataliya, Zharmukhamedova, Galiya, Doolotkeldieva, Tinatin, Smits, Theo H. M., Rezzonico, Fabio, "Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer" in Phytopathology Research, 3, no. 1 (2021):18,
https://doi.org/10.1186/s42483-021-00096-9 . .