TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5017
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier Sci Ltd, Oxford., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Andjelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4662
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - JOUR
AU  - Zdravković, Jasmina
AU  - Pavlović, Nenad V.
AU  - Bošković-Vragolović, Nevenka M.
AU  - Moravčević, Djordje
AU  - Šević, Milan
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4591
AB  - The aim of this study was to find an optimal way of tomato drying in a tunnel type dryer, in order to achieve the lowest possible losses in nutritive value of dried tomato products. Domestic variety of tomato (SP-109) was used in this research. Drying was performed in three ways, applying five temperature regimes, as follows: cocurrent system (variant 1 at 70-80 oC; variant 2 at 90-75 oC), countercurrent system (variant 1 at 55-65 oC, variant 2 at 65-75 oC) and combined system (85-55 oC and 55-65 oC). In these systems, the kinetic of changes in nutritive value of tomato fruits was monitored by measuring the content of carbohydrates, lycopene and vitamin C. Different influences of temperature regimes on nutritive value of dried tomato were observed at the level of statistical difference (LSD 0.05; 0.01) in the researched systems. Cocurrent system was statistically significant for differences in temperature modes (variant 1 and variant 2). Monitoring of the tomato drying kinetics showed that, in all variants, period of constant drying rate lasted about 3.5 h and that the total drying was the fastest in parallel cocurrent flow of non-saturated hot air and material. The content of total carbohydrates in tomato was dependent on the temperature regime of the tested drying systems. The carbohydrate content obtained in tomato samples dried at lower temperatures was higher compared to the values observed in the samples dried at higher drying temperatures. Significant losses of vitamin C were determined in all drying systems. The lycopene content under all experimental conditions generally showed a tendency to decrease slightly. Comparing its content in dry and fresh tomato fruits, the loss ranged from 4.94% to 19.98% but did not reach the significant level as the occurrence remained below 95% of cases.
AB  - Cilj ove studije bio je pronalaženje optimalnog načina sušenja paradajza u tunelskom tipu sušara kako bi se smanjili gubici vrednosti nutritijenata kod sušenog proizvoda paradajza. U ovom istraživanju korišćena je domaća sorta paradajza (SP-109). Istraživanje je izvedeno u tunelskom tipu sušara na tri načina u pet temperaturnih režima. Sušenje je izvedeno u sistemu paralelnog (varijanta 1 na 70-80 oC, varijanta 2 na 90-75 oC), suprotnosmernom (varijanta 1 na 55-65 oC, varijanta 2 na 65-75 oC) i kombinovanog sistema strujanja vazduha (85-55 oC i 55- 65 oC). U ovim sistemima sušenja praćena je kinetika promena nutritivnih vrednosti ploda paradajza: sadržaj ugljenih hidrata (%), likopena i askorbinske kiseline (vitamin C). Utvrđeni su različiti uticaji temperaturnih režima na nivou statističke razlike (LSD 0.05; 0.01) ispitivanih sistema za nutritivnu vrednost sušenog paradajza. Paralelni sistem je bio statistički značajan za razlike u varijanti temperature 1 i varijanti 2. Prateći kinetiku sušenja paradajza utvrđeno je da u svim varijantama period konstantnog sušenja traje oko 3,5 časa i da je za ukupno sušenje najbrži paralelni protok toplog vazduha i materijala. Sadržaj ugljenih hidrata u uzorcima paradajza koji su sušeni na nižim temperaturama bio je veći u poređenju sa sadržajem ugljenih hidrata koji je utvpđen u uzorcima osušenim na višim temperaturama sušenja. Na nižim temperaturama njihov sadržaj je bio veći nego kod viših temperatura sušenja. Značajni gubici vitamina C određeni su u svim sistemima sušenja. Vrednost likopena u svim eksperimentalnim uslovima sušenja pokazuje tendenciju blagog smanjenja. Poredeći sadržaj u suvim i svežim plodovima paradajza, gubitak je bio od 4,94% do 19,98%, ali ne na značajnom nivou.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Preservation of vitamin C, lycopene and carbohydrate content in tomato dried in a tunnel type dryer
T1  - Očuvanje vitamina C, likopena i ugljenih hidrata u plodovima paradajza sušenim u tunelskom tipu sušara
EP  - 142
IS  - 2
SP  - 133
VL  - 44
DO  - 10.5937/FFR1702133Z
ER  - 

@article{
author = "Zdravković, Jasmina and Pavlović, Nenad V. and Bošković-Vragolović, Nevenka M. and Moravčević, Djordje and Šević, Milan",
year = "2017",
abstract = "The aim of this study was to find an optimal way of tomato drying in a tunnel type dryer, in order to achieve the lowest possible losses in nutritive value of dried tomato products. Domestic variety of tomato (SP-109) was used in this research. Drying was performed in three ways, applying five temperature regimes, as follows: cocurrent system (variant 1 at 70-80 oC; variant 2 at 90-75 oC), countercurrent system (variant 1 at 55-65 oC, variant 2 at 65-75 oC) and combined system (85-55 oC and 55-65 oC). In these systems, the kinetic of changes in nutritive value of tomato fruits was monitored by measuring the content of carbohydrates, lycopene and vitamin C. Different influences of temperature regimes on nutritive value of dried tomato were observed at the level of statistical difference (LSD 0.05; 0.01) in the researched systems. Cocurrent system was statistically significant for differences in temperature modes (variant 1 and variant 2). Monitoring of the tomato drying kinetics showed that, in all variants, period of constant drying rate lasted about 3.5 h and that the total drying was the fastest in parallel cocurrent flow of non-saturated hot air and material. The content of total carbohydrates in tomato was dependent on the temperature regime of the tested drying systems. The carbohydrate content obtained in tomato samples dried at lower temperatures was higher compared to the values observed in the samples dried at higher drying temperatures. Significant losses of vitamin C were determined in all drying systems. The lycopene content under all experimental conditions generally showed a tendency to decrease slightly. Comparing its content in dry and fresh tomato fruits, the loss ranged from 4.94% to 19.98% but did not reach the significant level as the occurrence remained below 95% of cases., Cilj ove studije bio je pronalaženje optimalnog načina sušenja paradajza u tunelskom tipu sušara kako bi se smanjili gubici vrednosti nutritijenata kod sušenog proizvoda paradajza. U ovom istraživanju korišćena je domaća sorta paradajza (SP-109). Istraživanje je izvedeno u tunelskom tipu sušara na tri načina u pet temperaturnih režima. Sušenje je izvedeno u sistemu paralelnog (varijanta 1 na 70-80 oC, varijanta 2 na 90-75 oC), suprotnosmernom (varijanta 1 na 55-65 oC, varijanta 2 na 65-75 oC) i kombinovanog sistema strujanja vazduha (85-55 oC i 55- 65 oC). U ovim sistemima sušenja praćena je kinetika promena nutritivnih vrednosti ploda paradajza: sadržaj ugljenih hidrata (%), likopena i askorbinske kiseline (vitamin C). Utvrđeni su različiti uticaji temperaturnih režima na nivou statističke razlike (LSD 0.05; 0.01) ispitivanih sistema za nutritivnu vrednost sušenog paradajza. Paralelni sistem je bio statistički značajan za razlike u varijanti temperature 1 i varijanti 2. Prateći kinetiku sušenja paradajza utvrđeno je da u svim varijantama period konstantnog sušenja traje oko 3,5 časa i da je za ukupno sušenje najbrži paralelni protok toplog vazduha i materijala. Sadržaj ugljenih hidrata u uzorcima paradajza koji su sušeni na nižim temperaturama bio je veći u poređenju sa sadržajem ugljenih hidrata koji je utvpđen u uzorcima osušenim na višim temperaturama sušenja. Na nižim temperaturama njihov sadržaj je bio veći nego kod viših temperatura sušenja. Značajni gubici vitamina C određeni su u svim sistemima sušenja. Vrednost likopena u svim eksperimentalnim uslovima sušenja pokazuje tendenciju blagog smanjenja. Poredeći sadržaj u suvim i svežim plodovima paradajza, gubitak je bio od 4,94% do 19,98%, ali ne na značajnom nivou.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Preservation of vitamin C, lycopene and carbohydrate content in tomato dried in a tunnel type dryer, Očuvanje vitamina C, likopena i ugljenih hidrata u plodovima paradajza sušenim u tunelskom tipu sušara",
pages = "142-133",
number = "2",
volume = "44",
doi = "10.5937/FFR1702133Z"
}

Zdravković, J., Pavlović, N. V., Bošković-Vragolović, N. M., Moravčević, D.,& Šević, M.. (2017). Preservation of vitamin C, lycopene and carbohydrate content in tomato dried in a tunnel type dryer. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 44(2), 133-142.
https://doi.org/10.5937/FFR1702133Z

Zdravković J, Pavlović NV, Bošković-Vragolović NM, Moravčević D, Šević M. Preservation of vitamin C, lycopene and carbohydrate content in tomato dried in a tunnel type dryer. in Food and Feed Research. 2017;44(2):133-142.
doi:10.5937/FFR1702133Z .

Zdravković, Jasmina, Pavlović, Nenad V., Bošković-Vragolović, Nevenka M., Moravčević, Djordje, Šević, Milan, "Preservation of vitamin C, lycopene and carbohydrate content in tomato dried in a tunnel type dryer" in Food and Feed Research, 44, no. 2 (2017):133-142,
https://doi.org/10.5937/FFR1702133Z . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4543
AB  - Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency.
AB  - Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Control of pepper and tomato bacterial spot
T1  - Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova
EP  - 609
IS  - 6
SP  - 596
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4543
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato caused by Xanthomonas species, is one of the widespread and economically most important diseases of pepper and tomato. Due to the lack of resistant pepper and tomato genotypes, the emergence of new races of bacteria, and strains resistant to copper compounds, questionable seed quality and limited control practices, thesebacteria are limiting pepper production in Serbia. On tomato plants, under favourable weather conditions for disease development, these bacteria can cause major damage reflected in overall yield as well as in fruit quality. The protection of pepper and tomato, based on chemical methods such as use of copper-based compounds in combination with ethylene-bis-dithiocarbamates, do not provide a satisfactory disease control. Use of antibiotics in plant protection is not permitted in the EU as well as in Serbia. However, results of many authors show that satisfactory control of bacterial spot can be achieved by integrating positive effect of chemical treatments (copper compounds, systemic resistance activators) and various biological methods (antagonists, bacteriophages). However, nature of biological agents, as well as the specific mechanism of action of the systemic resistance activators, require careful optimization of the time and number of treatments in order to achieve maximum efficiency., Bakteriozna pegavost lista i krastavost plodova prouzrokovana Xanthomonas vrstama, je jedna od najrasprostranjenijih i ekonomski najznačajnijih bolesti paprike i paradajza u svetu. Usled nedostatka otpornih genotipova paprike i paradajza, pojave novih rasa bakterije, sojeva rezistentnih prema jedinjenjima bakra, sumnjivog kvaliteta semena i ograničenih mera kontrole, ova bakterioza predstavlja ograničavajući faktor proizvodnje paprike u Srbiji. Kada vremenski uslovi pogoduju razvoju bolesti na biljkama paradajza, ova bakterioza može prouzrokovati velike gubitke usled smanjenja ukupnog prinosa i kvaliteta plodova koji nisu pogodni za industrijsku preradu. U zaštiti paprike i paradajza kod nas dominiraju hemijske mere borbe, odnosno korišćenje preparata na bazi jedinjenja bakra u kombinaciji sa etilenbis-ditiokarbamatima koji ne obezbeđuju zadovoljavajući efekat zaštite. Antibiotici se ne mogu primenjivati usled zakonskih ograničenja u Evropskoj uniji. Integracijom pozitivnog efekta hemijskih metoda (bakarni preparati, aktivatori sistemične otpornosti) i različitih bioloških metoda (antagonostički sojevi bakterija, bakteriofagi) može se postići zadovoljavajući efekat zaštite. Priroda bioloških agenasa, kao i specifičan mehanizam dejstva aktivatora sistemične otpornosti, zahtevaju pažljivu optimizaciju vremena i broja tretmana kako bi se postigla maksimalna efikasnost.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Control of pepper and tomato bacterial spot, Zaštita paprike i paradajza od prouzrokovača bakteriozne pegavosti lista i krastavosti plodova",
pages = "609-596",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4543"
}

Šević, M., Gašić, K., Ignjatov, M.,& Obradović, A.. (2017). Control of pepper and tomato bacterial spot. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 596-609.
https://hdl.handle.net/21.15107/rcub_agrospace_4543

Šević M, Gašić K, Ignjatov M, Obradović A. Control of pepper and tomato bacterial spot. in Biljni lekar. 2017;45(6):596-609.
https://hdl.handle.net/21.15107/rcub_agrospace_4543 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Obradović, Aleksa, "Control of pepper and tomato bacterial spot" in Biljni lekar, 45, no. 6 (2017):596-609,
https://hdl.handle.net/21.15107/rcub_agrospace_4543 .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4542
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4542
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4542"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.
https://hdl.handle.net/21.15107/rcub_agrospace_4542

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596.
https://hdl.handle.net/21.15107/rcub_agrospace_4542 .

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596,
https://hdl.handle.net/21.15107/rcub_agrospace_4542 .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4541
AB  - Bacterial diseases are very common in tomato and pepper crops, especially under favourable weather conditions. One of the economically most important pathogens that affect tomato production worldwide is Ralstonia solanacearum, causal agent of bacterial wilt of tomato. The pathogen survives in soil and water. It colonizes xylem, clogs the vascular tissue preventing water movement throughout the stem, causing colapse of the infected plants. In addition, wilting of tomato and pepper can be caused by Candidatus Phytoplasma solani (Stolbur Phytoplasma). Main sources of infection with this pathogen are wild plants from where it is further transmitted by feeding of leafhopper vectors. The aim of this paper is to point to distribution and economic importance of those diseases, as well as characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, which contributes to the timely and successful protection strategy.
AB  - Oboljenja bakteriozne prirode veoma su česta u usevima paradajza i paprike, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski najznačajnijim bakterijama koje ugrožavaju proizvodnju paradajza u svetu, izdvaja se prouzrokovač bakteriozne uvelosti paradajza Ralstonia solanacearum. Patogen se dugo održava u zemljištu i vodi. Naseljava ksilem biljaka onemogućavajući protok vode, usled čega dolazi do pojave uvelosti. Osim ovog patogena, simptome uvelosti paradajza i paprike može takođe prouzrokovati Candidatus Phytoplasma solani (Stolbur fitoplazma). Ovo oboljenje prenosi se cikadama, dok su glavni izvori infekcije korovske biljke. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj navedenih oboljenja paradajza i paprike, kao i osnovne karakteristike njihovih prouzrokovača. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial wilt of tomato and Stolbur of tomato and pepper
T1  - Prouzrokovači bakteriozne uvelosti paradajza i žute uvelosti (Stolbur) paprike i paradajza
EP  - 586
IS  - 6
SP  - 575
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4541
ER  - 

@article{
author = "Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial diseases are very common in tomato and pepper crops, especially under favourable weather conditions. One of the economically most important pathogens that affect tomato production worldwide is Ralstonia solanacearum, causal agent of bacterial wilt of tomato. The pathogen survives in soil and water. It colonizes xylem, clogs the vascular tissue preventing water movement throughout the stem, causing colapse of the infected plants. In addition, wilting of tomato and pepper can be caused by Candidatus Phytoplasma solani (Stolbur Phytoplasma). Main sources of infection with this pathogen are wild plants from where it is further transmitted by feeding of leafhopper vectors. The aim of this paper is to point to distribution and economic importance of those diseases, as well as characteristics of the pathogens. Information about symptoms and epidemiology of the disease are important for an accurate disease diagnosis and pathogen identification, which contributes to the timely and successful protection strategy., Oboljenja bakteriozne prirode veoma su česta u usevima paradajza i paprike, posebno kada vremenski uslovi pogoduju nastanku i širenju infekcije. Među ekonomski najznačajnijim bakterijama koje ugrožavaju proizvodnju paradajza u svetu, izdvaja se prouzrokovač bakteriozne uvelosti paradajza Ralstonia solanacearum. Patogen se dugo održava u zemljištu i vodi. Naseljava ksilem biljaka onemogućavajući protok vode, usled čega dolazi do pojave uvelosti. Osim ovog patogena, simptome uvelosti paradajza i paprike može takođe prouzrokovati Candidatus Phytoplasma solani (Stolbur fitoplazma). Ovo oboljenje prenosi se cikadama, dok su glavni izvori infekcije korovske biljke. Cilj ovog rada je da se prikaže rasprostranjenost i ekonomski značaj navedenih oboljenja paradajza i paprike, kao i osnovne karakteristike njihovih prouzrokovača. Poznavanje simptomatologije i epidemiologije oboljenja je od posebnog značaja za pravilnu dijagnozu oboljenja i identifikaciju patogena, što doprinosi pravovremenoj i uspešnoj zaštiti.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial wilt of tomato and Stolbur of tomato and pepper, Prouzrokovači bakteriozne uvelosti paradajza i žute uvelosti (Stolbur) paprike i paradajza",
pages = "586-575",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4541"
}

Gašić, K., Šević, M.,& Obradović, A.. (2017). Bacterial wilt of tomato and Stolbur of tomato and pepper. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 575-586.
https://hdl.handle.net/21.15107/rcub_agrospace_4541

Gašić K, Šević M, Obradović A. Bacterial wilt of tomato and Stolbur of tomato and pepper. in Biljni lekar. 2017;45(6):575-586.
https://hdl.handle.net/21.15107/rcub_agrospace_4541 .

Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Bacterial wilt of tomato and Stolbur of tomato and pepper" in Biljni lekar, 45, no. 6 (2017):575-586,
https://hdl.handle.net/21.15107/rcub_agrospace_4541 .

TY  - JOUR
AU  - Stević, Milan
AU  - Pavlović, Biljana
AU  - Šević, Milan
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4271
AB  - During the season 2016, the biological efficacy of contact fungicides in apple protection from the apple scab, caused by fungus Venturia inaequalis, was performed. The experiments were conducted in an experimental orchard at the location of OŠD Radmilovac (cv Idared) and commercial orchard on the location of Krćevac (Topola) (cv Mucu). The trials were performed as a randomized block system in four repetitions (EPPO, 2012). Fungicide treatments were performed using knap sprayer type Solo 423, with 1000 l ha-1 of suspension consumption. A total of seven treatment, starting from the growth stages of ''mouse ear'' (BBCH 54), were done.Favorable agro-meteorological conditions have resulted in the significant disease incidence in control (untreated) plots. At the site of the OŠD Radmilovac the intensity of disease determined in control plots was 28.4% on the leaves, and 7.3% on fruits, while on the locality of Krćevac, diseases occurrence on the leaves and fruits was 91.5% and 7.9%, respectively. Application of multi-site fungicides resulted in good efficacy of mancozeb (87.4 to 100%), dithianon (83.1 to 100%), metiram (86.6 to 95.3%) and captan (89.8 to 100%), respectively.
AB  - U toku 2016. godine, vršena su ispitivanja biološke efikasnosti kontaknih fungicida u zaštiti jabuke od prouzrokovača čađave pegavosti lista i krastavosti plodova (Venturia inaequalis). Ogledi su izvedeni u eksperimentalnom zasadu na lokalitetu OŠD Radmilovac (sorta Ajdared) i komercijalnom zasadu na lokalitetu Krćevac (Topola), (sorta Mucu), po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 2012). Fungicidi su primenjivani pomoću leđnog atomizera, tipa Solo 423 uz utrošak vode 1000 l ha. Obavljeno je ukupno sedam tretiranja, počevši od fenofaze ''mišjih ušiju'' (BBCH 54) do fenofaze kada su plodovi bili Ø 8-12 mm (BBCH 70-71). Povoljni agrometeorološki uslovi rezultirali su značajnom pojavom oboljenja u kontrolnim parcelama. Na lokalitetu OŠD Radmilovac utvrđen je intezitet oboljenja u kontroli od 28,4% na listovima, odnosno 7,3% na plodovima, dok je na lokalitetu Krćevac, intezitet oboljenja na listu iznosio 91,5%, odnosno 7,9% na plodovima. Primenom fungicida sa kontaktim delovanjem ostvarena je veoma dobra efikasnost mankozeba (87,4-100%), ditianona (83,1-100%), metirama (86,6-95,3%) i kaptana (89,8-100%).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Effect of contact fungicides on management of Venturia inaequalis (Cooke) winter on apple
T1  - Efekti kontaktnih fungicida u zaštiti jabuke od Venturia inaequalis (Cooke) winter
EP  - 332
IS  - 4
SP  - 325
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4271
ER  - 

@article{
author = "Stević, Milan and Pavlović, Biljana and Šević, Milan",
year = "2016",
abstract = "During the season 2016, the biological efficacy of contact fungicides in apple protection from the apple scab, caused by fungus Venturia inaequalis, was performed. The experiments were conducted in an experimental orchard at the location of OŠD Radmilovac (cv Idared) and commercial orchard on the location of Krćevac (Topola) (cv Mucu). The trials were performed as a randomized block system in four repetitions (EPPO, 2012). Fungicide treatments were performed using knap sprayer type Solo 423, with 1000 l ha-1 of suspension consumption. A total of seven treatment, starting from the growth stages of ''mouse ear'' (BBCH 54), were done.Favorable agro-meteorological conditions have resulted in the significant disease incidence in control (untreated) plots. At the site of the OŠD Radmilovac the intensity of disease determined in control plots was 28.4% on the leaves, and 7.3% on fruits, while on the locality of Krćevac, diseases occurrence on the leaves and fruits was 91.5% and 7.9%, respectively. Application of multi-site fungicides resulted in good efficacy of mancozeb (87.4 to 100%), dithianon (83.1 to 100%), metiram (86.6 to 95.3%) and captan (89.8 to 100%), respectively., U toku 2016. godine, vršena su ispitivanja biološke efikasnosti kontaknih fungicida u zaštiti jabuke od prouzrokovača čađave pegavosti lista i krastavosti plodova (Venturia inaequalis). Ogledi su izvedeni u eksperimentalnom zasadu na lokalitetu OŠD Radmilovac (sorta Ajdared) i komercijalnom zasadu na lokalitetu Krćevac (Topola), (sorta Mucu), po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 2012). Fungicidi su primenjivani pomoću leđnog atomizera, tipa Solo 423 uz utrošak vode 1000 l ha. Obavljeno je ukupno sedam tretiranja, počevši od fenofaze ''mišjih ušiju'' (BBCH 54) do fenofaze kada su plodovi bili Ø 8-12 mm (BBCH 70-71). Povoljni agrometeorološki uslovi rezultirali su značajnom pojavom oboljenja u kontrolnim parcelama. Na lokalitetu OŠD Radmilovac utvrđen je intezitet oboljenja u kontroli od 28,4% na listovima, odnosno 7,3% na plodovima, dok je na lokalitetu Krćevac, intezitet oboljenja na listu iznosio 91,5%, odnosno 7,9% na plodovima. Primenom fungicida sa kontaktim delovanjem ostvarena je veoma dobra efikasnost mankozeba (87,4-100%), ditianona (83,1-100%), metirama (86,6-95,3%) i kaptana (89,8-100%).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Effect of contact fungicides on management of Venturia inaequalis (Cooke) winter on apple, Efekti kontaktnih fungicida u zaštiti jabuke od Venturia inaequalis (Cooke) winter",
pages = "332-325",
number = "4",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4271"
}

Stević, M., Pavlović, B.,& Šević, M.. (2016). Effect of contact fungicides on management of Venturia inaequalis (Cooke) winter on apple. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(4), 325-332.
https://hdl.handle.net/21.15107/rcub_agrospace_4271

Stević M, Pavlović B, Šević M. Effect of contact fungicides on management of Venturia inaequalis (Cooke) winter on apple. in Biljni lekar. 2016;44(4):325-332.
https://hdl.handle.net/21.15107/rcub_agrospace_4271 .

Stević, Milan, Pavlović, Biljana, Šević, Milan, "Effect of contact fungicides on management of Venturia inaequalis (Cooke) winter on apple" in Biljni lekar, 44, no. 4 (2016):325-332,
https://hdl.handle.net/21.15107/rcub_agrospace_4271 .

TY  - JOUR
AU  - Stević, Milan
AU  - Pavlović, Biljana
AU  - Šević, Milan
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4264
AB  - During the 2016 growing season, biological efficacy of fungicides mixture propamocarb hydrochloride + chlorothalonil, metalaxyl-M + mancozeb and metalksil-M + chlorothalonil in the control of downy mildew in pea crop (Peronospora viciae), was investigated. The experiments were conducted in the localities of Sakule and Glogonj. The experiments were carried out as randomized block system in four replications EPPO (EPPO, 1997). Two fugicide applications were conducted , one at the beginning of flowering (BBCH 61) (1st treatment), and second at the stage when 10% of pods reached typical size (BBCH 71) (2nd treatment). Favorable conditions resulted in significant disease intensity on untreated plots, which amounted from 19.9 to 24.8% in the first, and from 32.3 to 44.5% in the second assessment. Fungicide mixture propamocarb-hydrochloride + chlorothalonil exhibited efficacy ranging from 92.7-93.9% at the first and from 85.5 to 91.9% at the second assessment. Fungicide metalaxyl-M + mancozeb, reached the efficacy of 93.1-95.4% at the first, and 86.3-93.2% at the second assessment, while the highest efficacy was achieved by a combination metalaxyl-M + chlorothalonil, which ranged from 86.9 to 100% at the first, and from 86.9 to 95.3% at the second assessment.
AB  - U toku 2016. godine, vršena su ispitivanja biološke efikasnosti fungicida na bazi kombinacije propamokarb-hidrohlorid + hlorotalonil, metalaksil-M + mankozeb i metalksil-M + hlorotalonil u suzbijanju prouzrokovača plamenjače (Peronospora viciae) u usevu konzumnog graška. Ogledi su izvedeni na lokalitetima Sakule i Glogonj, po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 2004, 2012). Fungicidi su primenjeni dva puta, i to kada su biljke bile u fenofazi početka cvetanja (BBCH 61) (prvo tretiranje) i kada je 10% mahuna dostiglo tipičnu veličinu (BBCH 71) (drugo tretiranje). Povoljni agrometeorološki uslovi, rezultirali su značajnom pojavom oboljenja u kontroli koja je iznosila 19,9- 24,8% u vreme prve, odnosno, 32,3-44,5% u vreme druge ocene efekata. Primenom kombinacije fungicida propamokarb-hidrohlorid + hlorotalonil, ostvarena je efikasnost u iznosu 92,7-93,9% u prvoj, odnosno, 85,5-91,9%, u drugoj oceni. Kombinacijom fungicida metalaksil-M + mankozeb, postignuta je efikasnost od 93,1-95,4% u prvoj i 86,3-93,2% u drugoj oceni, dok je najbolja efikasnost postignuta primenom fungicida na bazi kombinacije metalaksil-M + hlorotalonil, koja je iznosila 86,9-100% u prvoj, odnosno, 86,9-95,3% u drugoj oceni.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Efficacy of fungicides in control of downy mildew (Peronospora viciae) in pea crop
T1  - Efikasnost fungicida u suzbijanju prouzrokovača plamenjače (Peronospora viciae) u usevu konzumnog graška
EP  - 230
IS  - 3
SP  - 224
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4264
ER  - 

@article{
author = "Stević, Milan and Pavlović, Biljana and Šević, Milan",
year = "2016",
abstract = "During the 2016 growing season, biological efficacy of fungicides mixture propamocarb hydrochloride + chlorothalonil, metalaxyl-M + mancozeb and metalksil-M + chlorothalonil in the control of downy mildew in pea crop (Peronospora viciae), was investigated. The experiments were conducted in the localities of Sakule and Glogonj. The experiments were carried out as randomized block system in four replications EPPO (EPPO, 1997). Two fugicide applications were conducted , one at the beginning of flowering (BBCH 61) (1st treatment), and second at the stage when 10% of pods reached typical size (BBCH 71) (2nd treatment). Favorable conditions resulted in significant disease intensity on untreated plots, which amounted from 19.9 to 24.8% in the first, and from 32.3 to 44.5% in the second assessment. Fungicide mixture propamocarb-hydrochloride + chlorothalonil exhibited efficacy ranging from 92.7-93.9% at the first and from 85.5 to 91.9% at the second assessment. Fungicide metalaxyl-M + mancozeb, reached the efficacy of 93.1-95.4% at the first, and 86.3-93.2% at the second assessment, while the highest efficacy was achieved by a combination metalaxyl-M + chlorothalonil, which ranged from 86.9 to 100% at the first, and from 86.9 to 95.3% at the second assessment., U toku 2016. godine, vršena su ispitivanja biološke efikasnosti fungicida na bazi kombinacije propamokarb-hidrohlorid + hlorotalonil, metalaksil-M + mankozeb i metalksil-M + hlorotalonil u suzbijanju prouzrokovača plamenjače (Peronospora viciae) u usevu konzumnog graška. Ogledi su izvedeni na lokalitetima Sakule i Glogonj, po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 2004, 2012). Fungicidi su primenjeni dva puta, i to kada su biljke bile u fenofazi početka cvetanja (BBCH 61) (prvo tretiranje) i kada je 10% mahuna dostiglo tipičnu veličinu (BBCH 71) (drugo tretiranje). Povoljni agrometeorološki uslovi, rezultirali su značajnom pojavom oboljenja u kontroli koja je iznosila 19,9- 24,8% u vreme prve, odnosno, 32,3-44,5% u vreme druge ocene efekata. Primenom kombinacije fungicida propamokarb-hidrohlorid + hlorotalonil, ostvarena je efikasnost u iznosu 92,7-93,9% u prvoj, odnosno, 85,5-91,9%, u drugoj oceni. Kombinacijom fungicida metalaksil-M + mankozeb, postignuta je efikasnost od 93,1-95,4% u prvoj i 86,3-93,2% u drugoj oceni, dok je najbolja efikasnost postignuta primenom fungicida na bazi kombinacije metalaksil-M + hlorotalonil, koja je iznosila 86,9-100% u prvoj, odnosno, 86,9-95,3% u drugoj oceni.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Efficacy of fungicides in control of downy mildew (Peronospora viciae) in pea crop, Efikasnost fungicida u suzbijanju prouzrokovača plamenjače (Peronospora viciae) u usevu konzumnog graška",
pages = "230-224",
number = "3",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4264"
}

Stević, M., Pavlović, B.,& Šević, M.. (2016). Efficacy of fungicides in control of downy mildew (Peronospora viciae) in pea crop. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(3), 224-230.
https://hdl.handle.net/21.15107/rcub_agrospace_4264

Stević M, Pavlović B, Šević M. Efficacy of fungicides in control of downy mildew (Peronospora viciae) in pea crop. in Biljni lekar. 2016;44(3):224-230.
https://hdl.handle.net/21.15107/rcub_agrospace_4264 .

Stević, Milan, Pavlović, Biljana, Šević, Milan, "Efficacy of fungicides in control of downy mildew (Peronospora viciae) in pea crop" in Biljni lekar, 44, no. 3 (2016):224-230,
https://hdl.handle.net/21.15107/rcub_agrospace_4264 .

TY  - CONF
AU  - Šević, Milan
AU  - Gašić, K.
AU  - Djordjević, M.
AU  - Ignjatov, Maja
AU  - Mijatović, Milan
AU  - Zecević, Bogoljub
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4051
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - VI Balkan Symposium on Vegetables and Potatoes
T1  - Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot
EP  - 150
SP  - 147
VL  - 1142
DO  - 10.17660/ActaHortic.2016.1142.23
ER  - 

@conference{
author = "Šević, Milan and Gašić, K. and Djordjević, M. and Ignjatov, Maja and Mijatović, Milan and Zecević, Bogoljub and Obradović, Aleksa",
year = "2016",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is considered one of the most important diseases of pepper in Serbia. In order to control this disease, copper based compounds (copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb), antibiotics (streptomycin sulfate and kasugamycin), resistance inducer (acibenzolar-S-methyl), antagonistic bacteria Bacillus subtilis (strains QST 713 and AAac), bacteriophage (strain K Phi 1) and one commercial microbial fertilizer, were tested for efficacy and compared to untreated control. The experiments were carried out in a greenhouse and climatic chamber conditions. Plants were inoculated with X euvesicatoria strain KFB 13 (10(8) CFU mL(-1)) using hand-held sprayer. Results of three experiments showed that all treatments except microbiological fertilizer significantly reduced intensity of bacterial spot symptoms compared to the untreated control. The most efficient treatment was acibenzolar-S-methyl reducing disease severity 93-97%. There was no statistically significant difference in efficacy of this treatment and copper hydroxide, copper oxychloride, copper hydroxide + mancozeb, copper oxychloride + mancozeb, streptomycin sulfate, kasugamycin and antagonistic strain QST 713 treatments. Application of antagonistic strain AAac and bacteriophage K Phi 1 significantly reduced disease intensity but less than the first group of treatments. Bacteriophage K Phi 1 (78-85% rH) was more effective in the climatic chamber compared to the efficacy (38% rH) in the greenhouse experiments.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "VI Balkan Symposium on Vegetables and Potatoes",
title = "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot",
pages = "150-147",
volume = "1142",
doi = "10.17660/ActaHortic.2016.1142.23"
}

Šević, M., Gašić, K., Djordjević, M., Ignjatov, M., Mijatović, M., Zecević, B.,& Obradović, A.. (2016). Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in VI Balkan Symposium on Vegetables and Potatoes
Int Soc Horticultural Science, Leuven 1., 1142, 147-150.
https://doi.org/10.17660/ActaHortic.2016.1142.23

Šević M, Gašić K, Djordjević M, Ignjatov M, Mijatović M, Zecević B, Obradović A. Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. in VI Balkan Symposium on Vegetables and Potatoes. 2016;1142:147-150.
doi:10.17660/ActaHortic.2016.1142.23 .

Šević, Milan, Gašić, K., Djordjević, M., Ignjatov, Maja, Mijatović, Milan, Zecević, Bogoljub, Obradović, Aleksa, "Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot" in VI Balkan Symposium on Vegetables and Potatoes, 1142 (2016):147-150,
https://doi.org/10.17660/ActaHortic.2016.1142.23 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3880
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3880
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3880"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

Zlatković, Nevena, Prokić, Andjelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271,
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

TY  - JOUR
AU  - Stević, Milan
AU  - Šević, Milan
AU  - Vukša, Petar
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3604
AB  - In 2012, the biological effi cacy of the fungicide mixture pyraclostrobin + boscalid (Signum®, BASF ) in the control of two economically most important disease of lettuce, gray mold (B. cinerea ) and downy mildew (B. lactucae ), was tested. The experiments were conducted at the location of Belgrade (Ledine, Iceberg, salat center), and Smederevska Palanka (Experimental fi eld of the Institute of Vegetable Crops), by type of randomized block design with four replications (EPPO, 1997). Fungicide application was performed by a sprayer SOLO 423 with consumption of 400 l of water ha-1. A fungicide Signum®, (a.i. pyraclostrobin + boscalid) was applied in a dosage of 1.5 kg ha-1 and was carried out a total of four treatments of the growth stages starting when the plants had developed three leaves (BBCH 13). As the standard products at both locations the fungicide Sumilex SC 50 (a. i. procymidone), in the case of B. cinerea, and Ridomil MZ (a.i. metalaxyl + mancozeb), in the case of for downy mildew, were used. After manifesting clear distinguish between treated and untreated (control) plots, assessment of disease severity (60 plants per plot) and efficiency of fungicides were calculated. The fungicide mixture pyraclostrobin + boscalid, tested in this study, showed a high efficacy against downy mildew of lettuce, which amounted to 93.6 % (Location: Belgrade - Ledine) and 97.4 % (site: Smederevska Palanka) respectively. The effi ciency of the tested fungicide combination of suppression of gray mold was 92.5 % (location: Belgrade - Ledine) and 93.5 % (site: Smederevska Palanka), respectively.
AB  - U toku 2012. godine, vršena su ispitivanja biološke efi kasnosti kombinacije fungicida piraklostrobin + boskalid (Signum®, BASF) u suzbijanju dve ekonomski najvažnije bolesti salate, sive truleži (Botrytis cinerea) i plamenjače (Bremia lactucae). Ogledi su izvedeni na lokalitetima Beograd (Ledine, Iceberg salat centar) i Smederevska Palanka (Ogledne parcele Instituta za povrtarstvo), po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 1997; EPPO, 2002). Aplikacija fungicida vršena je pomoću leđne prskalice uz utrošak vode od 400 l/ha. Ispitivani preparat Signum®, primenjen je u dozi od 1,5 kg/ha, a obavljeno je ukupno četiri tretiranja, počevši od fenofaze kada su biljke imale razvijena tri lista (BBCH 13). Kao standardni preparati za poređenje, na oba lokaliteta, korišćeni su Sumilex 50 SC (a.s. prosimidon), u slučaju B. cinerea, odnosno Ridomil gold MZ (a.s. metalaksil + mankozeb), za suzbijanje prouzrokovača plamenjače. Nakon ispoljavanja jasnih razlika između tretiranih varijanti i kontrole, ocenjen je intenzitet oboljenja (60 biljaka po jednoj parceli) i izračunata efi kasnost fungicida. Primenom ispitivane kombinacije piraklostrobin + boskalid, ostvarena je visoka efi kasnost u suzbijanju plamenjače salate koja je iznosila 93,6 % (lokalitet: Beograd-Ledine), odnosno, 97,4 % (lokalitet: Smederevska Palanka). Efi kasnost ispitivane kombinacije u suzbijanju sive truleži iznosila je 92,5 % (lokalitet: Beograd-Ledine), odnosno 93,5 % (lokalitet: Smederevska Palanka).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Efficacy of fungicide mixture (pyraclostrobin + boscalid) in gray mold and downy mildew on lettuce
T1  - Efikasnost kombinacije piraklostrobin + boskalid u suzbijanju sive truleži i plamenjače salate
EP  - 313
IS  - 4
SP  - 308
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3604
ER  - 

@article{
author = "Stević, Milan and Šević, Milan and Vukša, Petar",
year = "2014",
abstract = "In 2012, the biological effi cacy of the fungicide mixture pyraclostrobin + boscalid (Signum®, BASF ) in the control of two economically most important disease of lettuce, gray mold (B. cinerea ) and downy mildew (B. lactucae ), was tested. The experiments were conducted at the location of Belgrade (Ledine, Iceberg, salat center), and Smederevska Palanka (Experimental fi eld of the Institute of Vegetable Crops), by type of randomized block design with four replications (EPPO, 1997). Fungicide application was performed by a sprayer SOLO 423 with consumption of 400 l of water ha-1. A fungicide Signum®, (a.i. pyraclostrobin + boscalid) was applied in a dosage of 1.5 kg ha-1 and was carried out a total of four treatments of the growth stages starting when the plants had developed three leaves (BBCH 13). As the standard products at both locations the fungicide Sumilex SC 50 (a. i. procymidone), in the case of B. cinerea, and Ridomil MZ (a.i. metalaxyl + mancozeb), in the case of for downy mildew, were used. After manifesting clear distinguish between treated and untreated (control) plots, assessment of disease severity (60 plants per plot) and efficiency of fungicides were calculated. The fungicide mixture pyraclostrobin + boscalid, tested in this study, showed a high efficacy against downy mildew of lettuce, which amounted to 93.6 % (Location: Belgrade - Ledine) and 97.4 % (site: Smederevska Palanka) respectively. The effi ciency of the tested fungicide combination of suppression of gray mold was 92.5 % (location: Belgrade - Ledine) and 93.5 % (site: Smederevska Palanka), respectively., U toku 2012. godine, vršena su ispitivanja biološke efi kasnosti kombinacije fungicida piraklostrobin + boskalid (Signum®, BASF) u suzbijanju dve ekonomski najvažnije bolesti salate, sive truleži (Botrytis cinerea) i plamenjače (Bremia lactucae). Ogledi su izvedeni na lokalitetima Beograd (Ledine, Iceberg salat centar) i Smederevska Palanka (Ogledne parcele Instituta za povrtarstvo), po tipu slučajnog blok sistema u četiri ponavljanja (EPPO, 1997; EPPO, 2002). Aplikacija fungicida vršena je pomoću leđne prskalice uz utrošak vode od 400 l/ha. Ispitivani preparat Signum®, primenjen je u dozi od 1,5 kg/ha, a obavljeno je ukupno četiri tretiranja, počevši od fenofaze kada su biljke imale razvijena tri lista (BBCH 13). Kao standardni preparati za poređenje, na oba lokaliteta, korišćeni su Sumilex 50 SC (a.s. prosimidon), u slučaju B. cinerea, odnosno Ridomil gold MZ (a.s. metalaksil + mankozeb), za suzbijanje prouzrokovača plamenjače. Nakon ispoljavanja jasnih razlika između tretiranih varijanti i kontrole, ocenjen je intenzitet oboljenja (60 biljaka po jednoj parceli) i izračunata efi kasnost fungicida. Primenom ispitivane kombinacije piraklostrobin + boskalid, ostvarena je visoka efi kasnost u suzbijanju plamenjače salate koja je iznosila 93,6 % (lokalitet: Beograd-Ledine), odnosno, 97,4 % (lokalitet: Smederevska Palanka). Efi kasnost ispitivane kombinacije u suzbijanju sive truleži iznosila je 92,5 % (lokalitet: Beograd-Ledine), odnosno 93,5 % (lokalitet: Smederevska Palanka).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Efficacy of fungicide mixture (pyraclostrobin + boscalid) in gray mold and downy mildew on lettuce, Efikasnost kombinacije piraklostrobin + boskalid u suzbijanju sive truleži i plamenjače salate",
pages = "313-308",
number = "4",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3604"
}

Stević, M., Šević, M.,& Vukša, P.. (2014). Efficacy of fungicide mixture (pyraclostrobin + boscalid) in gray mold and downy mildew on lettuce. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 308-313.
https://hdl.handle.net/21.15107/rcub_agrospace_3604

Stević M, Šević M, Vukša P. Efficacy of fungicide mixture (pyraclostrobin + boscalid) in gray mold and downy mildew on lettuce. in Biljni lekar. 2014;42(4):308-313.
https://hdl.handle.net/21.15107/rcub_agrospace_3604 .

Stević, Milan, Šević, Milan, Vukša, Petar, "Efficacy of fungicide mixture (pyraclostrobin + boscalid) in gray mold and downy mildew on lettuce" in Biljni lekar, 42, no. 4 (2014):308-313,
https://hdl.handle.net/21.15107/rcub_agrospace_3604 .

TY  - JOUR
AU  - Gašić, K.
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3510
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 

@article{
author = "Gašić, K. and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}

Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae
International Society for Horticultural Science., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17

Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .

Gašić, K., Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3603
AB  - Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control.
AB  - Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - New strategies for pepper and tomato bacterial spot control
T1  - Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti
EP  - 307
IS  - 4
SP  - 296
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3603
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Obradović, Aleksa",
year = "2014",
abstract = "Bacterial spot, caused by bacteria that belong to Xanthomonas complex, is one of the widespread and economically important disease of pepper and tomato. Growing resistant genotypes, use of treatments based on copper compounds or streptomycin do not provide efficient control of this disease due to the appearance of new races of bacteria and development of strains resistant to antibiotics and copper compounds. Pepper and tomato producers do not have adequate resources for pathogen control since standard bactericides are not effective enough if weather conditions are favorable for the disease development. Due to lack of resistant cultivars and effective means of protection, researchers have searched for alternative solutions that would provide effective control of this disease. Only integration of different control methods can enable positive effect and achieve an adequate crop protection. Biological methods (use of bacteriophages) and some recent alternative treatments (Systemic Acquired Resistance inducers), have provided the possibility of developing an effective strategy for X. euvesicatoria control., Bakteriozna pegavost paprike i krastavost plodova paradajza koju prouzrokuju bakterije Xanthomonas kompleksa, spada u red rasprostranjenih i ekonomski veoma značajnih bolesti paprike i paradajza. Gajenje otpornih genotipova i primena preparata na bazi streptomicina i jedinjenja bakra ne obezbeđuju zadovoljavajući efekat zaštite, usled pojave novih rasa bakterije i razvoja sojeva rezistentnih prema antibioticima i jedinjenjima bakra. Proizvođači paprike i paradajza nemaju adekvatna sredstva za borbu protiv ovog patogena, jer standardni baktericidi često nisu dovoljno efi kasni kada vremenski uslovi pogoduju razvoju bolesti. Usled nedostatka otpornih sorti, kao i efi kasnih sredstava za zaštitu, istraživači pokušavaju da pronađu alternativna rešenja kojima bi se omogućila efi kasna kontrola ove bolesti. Jedino se integracijom pozitivnog efekta različitih metoda može postići odgovarajući efekat zaštite. Biološke metode (primena bakteriofaga) i neke novije alternativne metode (aktivatori sistemične opornosti), ukazuju na mogućnost razvoja efi kasne strategije za suzbijanje X. euvesicatoria.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "New strategies for pepper and tomato bacterial spot control, Novi pravci zaštite paprike i paradajza od bakteriozne pegavosti",
pages = "307-296",
number = "4",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3603"
}

Šević, M., Gašić, K.,& Obradović, A.. (2014). New strategies for pepper and tomato bacterial spot control. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603

Šević M, Gašić K, Obradović A. New strategies for pepper and tomato bacterial spot control. in Biljni lekar. 2014;42(4):296-307.
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .

Šević, Milan, Gašić, Katarina, Obradović, Aleksa, "New strategies for pepper and tomato bacterial spot control" in Biljni lekar, 42, no. 4 (2014):296-307,
https://hdl.handle.net/21.15107/rcub_agrospace_3603 .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2937
AB  - Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease.
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Sensitivity of some pepper genotypes to bacterial spot causal agent
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease., Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Sensitivity of some pepper genotypes to bacterial spot causal agent, Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Sensitivity of some pepper genotypes to bacterial spot causal agent" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Šević, Milan
AU  - Obradović, Aleksa
AU  - Milošević, Mirjana
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2216
AB  - During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.
AB  - Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia
T1  - Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji
EP  - 149
IS  - 2
SP  - 139
VL  - 25
DO  - 10.2298/PIF1002139I
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Ivanović, Milan and Šević, Milan and Obradović, Aleksa and Milošević, Mirjana",
year = "2010",
abstract = "During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development., Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia, Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji",
pages = "149-139",
number = "2",
volume = "25",
doi = "10.2298/PIF1002139I"
}

Ignjatov, M., Gašić, K., Ivanović, M., Šević, M., Obradović, A.,& Milošević, M.. (2010). Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(2), 139-149.
https://doi.org/10.2298/PIF1002139I

Ignjatov M, Gašić K, Ivanović M, Šević M, Obradović A, Milošević M. Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina. 2010;25(2):139-149.
doi:10.2298/PIF1002139I .

Ignjatov, Maja, Gašić, Katarina, Ivanović, Milan, Šević, Milan, Obradović, Aleksa, Milošević, Mirjana, "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia" in Pesticidi i fitomedicina, 25, no. 2 (2010):139-149,
https://doi.org/10.2298/PIF1002139I . .