TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Visnjić-Jeftić, Zeljka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Djikanović, Vesna
AU  - Jarić, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3690
AB  - The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.
PB  - Wiley, Hoboken
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution
EP  - 1161
IS  - 10
SP  - 1153
VL  - 30
DO  - 10.1002/tox.21988
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Visnjić-Jeftić, Zeljka and Skorić, Stefan and Gačić, Zoran and Djikanović, Vesna and Jarić, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.",
publisher = "Wiley, Hoboken",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution",
pages = "1161-1153",
number = "10",
volume = "30",
doi = "10.1002/tox.21988"
}

Rašković, B., Poleksić, V., Visnjić-Jeftić, Z., Skorić, S., Gačić, Z., Djikanović, V., Jarić, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology
Wiley, Hoboken., 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988

Rašković B, Poleksić V, Visnjić-Jeftić Z, Skorić S, Gačić Z, Djikanović V, Jarić I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .

Rašković, Božidar, Poleksić, Vesna, Visnjić-Jeftić, Zeljka, Skorić, Stefan, Gačić, Zoran, Djikanović, Vesna, Jarić, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Rašković, Božidar
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
AU  - Gačić, Zoran
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3781
AB  - Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.
PB  - University of Ljubljana - Veterinary Faculty
T2  - Slovenian Veterinary Research
T1  - Integrated use of different fish related parameters to assess the status of water bodies
EP  - 13
IS  - 1
SP  - 5
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3781
ER  - 

@article{
author = "Lenhardt, Mirjana and Poleksić, Vesna and Vuković-Gačić, Branka and Rašković, Božidar and Sunjog, Karolina and Kolarević, Stoimir and Jarić, Ivan and Gačić, Zoran",
year = "2015",
abstract = "Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.",
publisher = "University of Ljubljana - Veterinary Faculty",
journal = "Slovenian Veterinary Research",
title = "Integrated use of different fish related parameters to assess the status of water bodies",
pages = "13-5",
number = "1",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3781"
}

Lenhardt, M., Poleksić, V., Vuković-Gačić, B., Rašković, B., Sunjog, K., Kolarević, S., Jarić, I.,& Gačić, Z.. (2015). Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research
University of Ljubljana - Veterinary Faculty., 52(1), 5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781

Lenhardt M, Poleksić V, Vuković-Gačić B, Rašković B, Sunjog K, Kolarević S, Jarić I, Gačić Z. Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research. 2015;52(1):5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .

Lenhardt, Mirjana, Poleksić, Vesna, Vuković-Gačić, Branka, Rašković, Božidar, Sunjog, Karolina, Kolarević, Stoimir, Jarić, Ivan, Gačić, Zoran, "Integrated use of different fish related parameters to assess the status of water bodies" in Slovenian Veterinary Research, 52, no. 1 (2015):5-13,
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Jarić, Ivan
AU  - Koko, Vesna
AU  - Spasić, Milan
AU  - Dulić, Zorka
AU  - Marković, Zoran
AU  - Poleksić, Vesna
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3315
AB  - In order to evaluate the relationship between water quality in ponds and indices of histopathological changes occurring in the vital organs of the common carp (Cyprinus carpio L., 1758), two six-month field experiments were carried out using two different water supplies: from the nearby stream and a tube well. The fish were fed supplemental feed: raw cereals, pelleted and extruded compound feed. Histopathological analysis, alteration frequencies, and semi-quantitative scoring of the changes were used to assess the health status of the fish. Ponds supplied by stream water were characterized by higher water hardness, dissolved oxygen and pH values, while those supplied by the tube well had higher electroconductivity, total ammonium and orthophosphates content. Fish survival rate and habitat suitability index were lower in ponds supplied by stream water, while the weight gain did not differ between the two water supplies. The use of stream water resulted in a higher level of histopathological changes in gills and liver. Among the water quality parameters, pH level had the strongest influence on fish. Differences in water supply produced greater influence on the level of histopathological changes than the type of feed applied. Gills were the most sensitive organ, while the kidney was the least responsive.
PB  - Sciendo, Warsaw
T2  - Central European Journal of Biology
T1  - Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture
EP  - 985
IS  - 10
SP  - 975
VL  - 8
DO  - 10.2478/s11535-013-0220-y
ER  - 

@article{
author = "Rašković, Božidar and Jarić, Ivan and Koko, Vesna and Spasić, Milan and Dulić, Zorka and Marković, Zoran and Poleksić, Vesna",
year = "2013",
abstract = "In order to evaluate the relationship between water quality in ponds and indices of histopathological changes occurring in the vital organs of the common carp (Cyprinus carpio L., 1758), two six-month field experiments were carried out using two different water supplies: from the nearby stream and a tube well. The fish were fed supplemental feed: raw cereals, pelleted and extruded compound feed. Histopathological analysis, alteration frequencies, and semi-quantitative scoring of the changes were used to assess the health status of the fish. Ponds supplied by stream water were characterized by higher water hardness, dissolved oxygen and pH values, while those supplied by the tube well had higher electroconductivity, total ammonium and orthophosphates content. Fish survival rate and habitat suitability index were lower in ponds supplied by stream water, while the weight gain did not differ between the two water supplies. The use of stream water resulted in a higher level of histopathological changes in gills and liver. Among the water quality parameters, pH level had the strongest influence on fish. Differences in water supply produced greater influence on the level of histopathological changes than the type of feed applied. Gills were the most sensitive organ, while the kidney was the least responsive.",
publisher = "Sciendo, Warsaw",
journal = "Central European Journal of Biology",
title = "Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture",
pages = "985-975",
number = "10",
volume = "8",
doi = "10.2478/s11535-013-0220-y"
}

Rašković, B., Jarić, I., Koko, V., Spasić, M., Dulić, Z., Marković, Z.,& Poleksić, V.. (2013). Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture. in Central European Journal of Biology
Sciendo, Warsaw., 8(10), 975-985.
https://doi.org/10.2478/s11535-013-0220-y

Rašković B, Jarić I, Koko V, Spasić M, Dulić Z, Marković Z, Poleksić V. Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture. in Central European Journal of Biology. 2013;8(10):975-985.
doi:10.2478/s11535-013-0220-y .

Rašković, Božidar, Jarić, Ivan, Koko, Vesna, Spasić, Milan, Dulić, Zorka, Marković, Zoran, Poleksić, Vesna, "Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture" in Central European Journal of Biology, 8, no. 10 (2013):975-985,
https://doi.org/10.2478/s11535-013-0220-y . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Poleksić, Vesna
AU  - Višnjić-Jeftić, Z.
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2414
AB  - Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.
PB  - Vasile Goldis Western University, Arad
T2  - Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
T1  - Ecological status of Serbian rivers based on an ichthyological assessment
EP  - 860
IS  - 4
SP  - 855
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2414
ER  - 

@article{
author = "Lenhardt, Mirjana and Gačić, Zoran and Vuković-Gačić, Branka and Poleksić, Vesna and Višnjić-Jeftić, Z. and Kolarević, Stoimir and Jarić, Ivan",
year = "2011",
abstract = "Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.",
publisher = "Vasile Goldis Western University, Arad",
journal = "Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii",
title = "Ecological status of Serbian rivers based on an ichthyological assessment",
pages = "860-855",
number = "4",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2414"
}

Lenhardt, M., Gačić, Z., Vuković-Gačić, B., Poleksić, V., Višnjić-Jeftić, Z., Kolarević, S.,& Jarić, I.. (2011). Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Vasile Goldis Western University, Arad., 21(4), 855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414

Lenhardt M, Gačić Z, Vuković-Gačić B, Poleksić V, Višnjić-Jeftić Z, Kolarević S, Jarić I. Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii. 2011;21(4):855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .

Lenhardt, Mirjana, Gačić, Zoran, Vuković-Gačić, Branka, Poleksić, Vesna, Višnjić-Jeftić, Z., Kolarević, Stoimir, Jarić, Ivan, "Ecological status of Serbian rivers based on an ichthyological assessment" in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii, 21, no. 4 (2011):855-860,
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .

TY  - CONF
AU  - Poleksić, Vesna
AU  - Lenhardt, Mirjana
AU  - Jarić, Ivan
AU  - Djordjević, Dragana
AU  - Gačić, Zoran
AU  - Cvijanović, Gorcin
AU  - Rašković, Božidar
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2377
AB  - The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations. Environ. Toxicol. Chem. 2010; 29: 515-521.
PB  - Setac Press, Pensacola
C3  - Environmental Toxicology and Chemistry
T1  - Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)
EP  - 521
IS  - 3
SP  - 515
VL  - 29
DO  - 10.1002/etc.82
ER  - 

@conference{
author = "Poleksić, Vesna and Lenhardt, Mirjana and Jarić, Ivan and Djordjević, Dragana and Gačić, Zoran and Cvijanović, Gorcin and Rašković, Božidar",
year = "2010",
abstract = "The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations. Environ. Toxicol. Chem. 2010; 29: 515-521.",
publisher = "Setac Press, Pensacola",
journal = "Environmental Toxicology and Chemistry",
title = "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)",
pages = "521-515",
number = "3",
volume = "29",
doi = "10.1002/etc.82"
}

Poleksić, V., Lenhardt, M., Jarić, I., Djordjević, D., Gačić, Z., Cvijanović, G.,& Rašković, B.. (2010). Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry
Setac Press, Pensacola., 29(3), 515-521.
https://doi.org/10.1002/etc.82

Poleksić V, Lenhardt M, Jarić I, Djordjević D, Gačić Z, Cvijanović G, Rašković B. Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry. 2010;29(3):515-521.
doi:10.1002/etc.82 .

Poleksić, Vesna, Lenhardt, Mirjana, Jarić, Ivan, Djordjević, Dragana, Gačić, Zoran, Cvijanović, Gorcin, Rašković, Božidar, "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)" in Environmental Toxicology and Chemistry, 29, no. 3 (2010):515-521,
https://doi.org/10.1002/etc.82 . .