TY  - JOUR
AU  - Radosavljević, Milos
AU  - Lević, Steva
AU  - Belović, Miona
AU  - Pejin, Jelena
AU  - Djukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Nedović, Viktor
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5353
AB  - Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using "freezing-thawing" technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability, L. rhamnosus cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g(-1)) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L-1 h(-1), respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L-1 h(-1). This study suggested that the "freezing-thawing" technique can be successfully used for immobilization of L. rhamnosus in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid
EP  - 322
IS  - 2
SP  - 315
VL  - 43
DO  - 10.1007/s00449-019-02228-0
ER  - 

@article{
author = "Radosavljević, Milos and Lević, Steva and Belović, Miona and Pejin, Jelena and Djukić-Vuković, Aleksandra and Mojović, Ljiljana and Nedović, Viktor",
year = "2020",
abstract = "Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using "freezing-thawing" technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability, L. rhamnosus cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g(-1)) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L-1 h(-1), respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L-1 h(-1). This study suggested that the "freezing-thawing" technique can be successfully used for immobilization of L. rhamnosus in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid",
pages = "322-315",
number = "2",
volume = "43",
doi = "10.1007/s00449-019-02228-0"
}

Radosavljević, M., Lević, S., Belović, M., Pejin, J., Djukić-Vuković, A., Mojović, L.,& Nedović, V.. (2020). Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid. in Bioprocess and Biosystems Engineering
Springer, New York., 43(2), 315-322.
https://doi.org/10.1007/s00449-019-02228-0

Radosavljević M, Lević S, Belović M, Pejin J, Djukić-Vuković A, Mojović L, Nedović V. Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid. in Bioprocess and Biosystems Engineering. 2020;43(2):315-322.
doi:10.1007/s00449-019-02228-0 .

Radosavljević, Milos, Lević, Steva, Belović, Miona, Pejin, Jelena, Djukić-Vuković, Aleksandra, Mojović, Ljiljana, Nedović, Viktor, "Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid" in Bioprocess and Biosystems Engineering, 43, no. 2 (2020):315-322,
https://doi.org/10.1007/s00449-019-02228-0 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Nedović, Viktor
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2039
AB  - BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 503
IS  - 4
SP  - 497
VL  - 84
DO  - 10.1002/jctb.2068
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Nedović, Viktor",
year = "2009",
abstract = "BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "503-497",
number = "4",
volume = "84",
doi = "10.1002/jctb.2068"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Nedović, V.. (2009). Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 84(4), 497-503.
https://doi.org/10.1002/jctb.2068

Nikolić S, Mojović L, Rakin M, Pejin D, Nedović V. Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology. 2009;84(4):497-503.
doi:10.1002/jctb.2068 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Nedović, Viktor, "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Journal of Chemical Technology and Biotechnology, 84, no. 4 (2009):497-503,
https://doi.org/10.1002/jctb.2068 . .

TY  - CONF
AU  - Mojović, Ljiljana
AU  - Nedović, Viktor
AU  - Rakin, Marica
AU  - Nikolić, Svetlana
AU  - Lević, Steva
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1619
AB  - Bioethanol can be produced by fermentation of sugars from waste agricultural materials. In this process immobilized yeast offer advantage over free cell suspension with regard to ethanol productivity, fermentation rate and product inhibition. The aim of the present study was to investigate the immobilization of Sacharomyces cerevisiae var. ellipsoideus yeast cells for bioethanol production from corn meal hydrolyzates. For this purpose the biocompatible polymers such as: PVA and Ca-alginate were assessed. The conditions of ethanol fermentation, such as inoculum concentration in different carriers, fermentation rate and the choice of a convenient carrier for the efficient ethanol production were studied, The maximum ethanol concentration of 10.05% (w/w) was obtained in the fermentation of corn meal hydrolyzates by 5% (v/v) inoculum concentration of the yeast immobilized in Ca-alginate using a method of electrostatic droplet generation. The repeated batch fermentation with the yeast immobilized in Ca-alginate at an initial sugar concentration of 176g/l, indicated that alginate gels degraded after the second fermentation cycle and 76 hours. In contrast, PVA carrier exhibited better mechanical properties and stability, however lower ethanol concentrations were achieved during the fermentation.
PB  - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
C3  - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
T1  - Bioethanol Production from Corn Meal Hydrolyzates by Immobilized Saccharomyces cerevisiae var. ellipsoideus
EP  - 349
SP  - 343
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1619
ER  - 

@conference{
author = "Mojović, Ljiljana and Nedović, Viktor and Rakin, Marica and Nikolić, Svetlana and Lević, Steva",
year = "2008",
abstract = "Bioethanol can be produced by fermentation of sugars from waste agricultural materials. In this process immobilized yeast offer advantage over free cell suspension with regard to ethanol productivity, fermentation rate and product inhibition. The aim of the present study was to investigate the immobilization of Sacharomyces cerevisiae var. ellipsoideus yeast cells for bioethanol production from corn meal hydrolyzates. For this purpose the biocompatible polymers such as: PVA and Ca-alginate were assessed. The conditions of ethanol fermentation, such as inoculum concentration in different carriers, fermentation rate and the choice of a convenient carrier for the efficient ethanol production were studied, The maximum ethanol concentration of 10.05% (w/w) was obtained in the fermentation of corn meal hydrolyzates by 5% (v/v) inoculum concentration of the yeast immobilized in Ca-alginate using a method of electrostatic droplet generation. The repeated batch fermentation with the yeast immobilized in Ca-alginate at an initial sugar concentration of 176g/l, indicated that alginate gels degraded after the second fermentation cycle and 76 hours. In contrast, PVA carrier exhibited better mechanical properties and stability, however lower ethanol concentrations were achieved during the fermentation.",
publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists",
journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20",
title = "Bioethanol Production from Corn Meal Hydrolyzates by Immobilized Saccharomyces cerevisiae var. ellipsoideus",
pages = "349-343",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1619"
}

Mojović, L., Nedović, V., Rakin, M., Nikolić, S.,& Lević, S.. (2008). Bioethanol Production from Corn Meal Hydrolyzates by Immobilized Saccharomyces cerevisiae var. ellipsoideus. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 2, 343-349.
https://hdl.handle.net/21.15107/rcub_agrospace_1619

Mojović L, Nedović V, Rakin M, Nikolić S, Lević S. Bioethanol Production from Corn Meal Hydrolyzates by Immobilized Saccharomyces cerevisiae var. ellipsoideus. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;2:343-349.
https://hdl.handle.net/21.15107/rcub_agrospace_1619 .

Mojović, Ljiljana, Nedović, Viktor, Rakin, Marica, Nikolić, Svetlana, Lević, Steva, "Bioethanol Production from Corn Meal Hydrolyzates by Immobilized Saccharomyces cerevisiae var. ellipsoideus" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2 (2008):343-349,
https://hdl.handle.net/21.15107/rcub_agrospace_1619 .